Child ; Female ; Humans ; Job Syndrome

Objective: To establish a Beagle dog model for the study of the mechanism of implant-bone interface remodeling following the restoration of submerged and nonsubmerged implant denture. Methods: 8 adult purebred beagle dogs were used to build the animal model. Fixed metal full crown was used to carry out submerged and nonsubmerged implant denture in the dogs to mimic the normal chewing status in animal models. Results: The submerged and nonsubmerged implant dentures were made successfully. After the implant denture restoration, all experimental dogs were fed with granular horniness forage to guarantee enough biting stimulation. The chewing fashion of experimental dogs was not changed obviously during the experiment process. Although the horniness forage may result in fairly bigger bite force, no visble biting hurt was found 12 weeks after loading and the fixed implant dentures were all preserved. Conclusion: The beagle dog model can preferably mimic the normal chewing fashion and the models are both availability and credibility.

Objective To investigate the respiratory function of platelet mitochondria in patients with Parkinson's disease(PD) and explore the assosiation with the pathogenesis of PD.Methods The platelet mitochondria of 35 patients with PD were extracted and the mitochondria respiratory rate and respiratory control rate were measured.Furthermore,the changes of activities of mitochondria complex Ⅰ,Ⅱ,Ⅲ and Ⅳ were observed.Results The platelet counts of patients with PD showed no changes, but the respiratory rates of respiratory state 3 were significantly decreased using the 5 mmol/L glutamate,2.5 mmol/L malate +5 mmol/L pyruvate and 5 mM succinate as substrates. Moreover, the activities of mitochondria complex Ⅰ were obviously reduced and the activites of other mitochondria complex were no changes.Conclusion Patients with PD show exactly abnormal mitochondria respiratory functions, and it might be an important mechanism in the pathogenesis of PD.

Objective To observe the function changes of right ventricular in chronic obstructive emphysema after lung volume reduction surgery. Methods Twenty-six patients with chronic obstructive emphysema undergoing lung volume reduction surgery were examined by color Doppler echocardiography to determine systolic function and diastolic function of right ventricle in preoperative days and postoperative days of 5～7; 12～14; 1 month and 3 months. Results Contraction of right ventricle: comparing with preoperative parameters, there were significant decrease (P0.05) of those above mentioned in postoperative days 12～14. It recovered at 3 month after operation. Relaxation of right ventricle: the swift filling peak flow-rate in earlier relax period (E) and E flow-rate integra (ETVI) decreased meanwhile swif filling peak flow-rate in late relax period (A) and A flow-rate integra (ATVI) increased in the postoperative days 5～7 (P

Objective: To study the implant-bone interface remodelin g after loading of submerged and nonsubmerged implant denture.Methods: 8 adult beagle dogs were used to build the animal model. Submerged and nons ubmerged implants were implanted into the bilateral mandible. Fixed metal full c rown was used to carry out the submerged and nonsubmerged implant denture. The b eagle dogs were sacrificed by steps after loading of the dentures. HE staining t echnique was used to observe the dynamic remodeling process of implant-bone int erface.Results:2 weeks after loading of implant denture, a major ity of the implant surface attached to bone tissue directly, however, at the int erface, especially at the top of the screw thread, bone tissues were absorbed an d substituted by fibrous tissues. 4 weeks after loading, fine attachment was fou nd at the implant-bone interface and the previouly observed fibrous tissue at t he interface was gradually remodeled to form new bone. 8 weeks after loading, i mplant directly attached to bone tissues by osteo-interface, and the cellular c omponents and capillaries were decreased at the interface.12 weeks after loading , all implants attached to bone tissues by osteo-interface with high combinatio n level, typical Havers system was observed at the interface.No obvious differen ce in the interface remodeling was observed between the submerged and nonsubmerg ed implant denture. Conclusion:There is no distinct difference i n the implant-bone interface remodeling after the loading of submerged and nons ubmerged implant denture.

Objective The analysis healthy crowd skull magnetic resonance image formation(MRI) inspection result,understood healthy crowd skull common disease the type,favors the healthy physical examination project the reasonable establishment.Methods Medical Center sent medical examination of 380 cases of brain MRI data and retrospective analysis.Results 380 cases of brain MRI results show healthy physical examination:there are 9 kinds of disease,MRI exception occurred there were 109 cases,abnormal rate of 28.7％ ; by age is divided into three groups:30-40 sui,41-50 sui; 51-60 sui; 51-60 sui exception rate was highest in the age group,to 56.9％ ; the discovery of the disease,the highest incidence of disease with multiple lacunar cerebral infarction,73.4％ per cent of total incidence.Conclusion MRI no radiation,brain MRI examination should as a general project of middle and old aged healthy,early detection of brain tumor lesions and cerebral vascular lesion,disease prevention and healthy living for guidance.

OBJECTIVE To investigate the correlations on hepatitis B virus(HBV)preS1-antigen(preS1-Ag)and HBV-DNA,HBV markers in patients with hepatitis B.METHODS The markers,preS1-Ag and HBV-DNA were determined by ELISA and PCR in 406 patients with hepatitis B and 80 healthy persons.RESULTS The positive rates of preS1-Ag in 160 patients with HBsAg(+)and HBeAg(+)were 84.4%.The positive rates of preS1-Ag in 246 patients with HBsAg(+)and HBeAg(-)were 42.3%;the difference between them was significant(?2=70.9,P

Objective To establish an animal model to study endometriosis. Methods Six female Bama miniature pigs that were sexually mature were chosen and their endometrial tissues were acquired surgically. The endometrial tissues were implanted to abdominal cavity and subcutaneous tissues. Results The ectopic endometria in the implant location formed mass or cyst that was verified pathologically to be similar to normal endometrial tissues. Conclusion The establishment of ectopic endometriosis in Bama miniature pigs is successful, which provides an ideal model for further therapeutic study of endometriosis.

Objective To investigate the effects of nordihydroguaiaretic acid(NDGA)on the endometriosis established in Bama miniature pigs.Methods Six Bama pigs that had been successfully established into animal model of endometriosis subcutaneously received 3% NDGA at dose of 20 mg/kg(n=4)or PBS solution(n=2)for 20 d.The grafts in abdominal cavity and subcutaneous tissues were observed and the serum concentrations of hormone,were measured by chemiluminoimmunoassay before and after the period of NDGA or PBS injection.The expressions of FⅧAg and VEGF in the endometriotic tissues were observed.Results The endometriotic masses were reduced to some degree after the time period of NDGA injection,even partially disappeared,while those treated with PBS showed no obvious changes.Histoimmunochemical analysis showed NDGA decreased the number of endometrial glands,the proliferation rate of the glandular epithelial cells,the endometrial stroma cells and the vessels,and inhibited the microvascular density and VEGF in the endometriotic tissues(P

Objective To determine whether normal-appearing cervical spinal cord in patients with amyotrophic lateral sclerosis (ALS) has abnormal changes based on the quantitative measurement in healthy volunteers. Methods Conventional MRI and axial DTI were obtained in 16 patients with ALS (ALS group) and 16 age-matched control subjects (normal group) . ADC, fractional anisotropy (FA) and relative anisotropy(RA)imagcs were obtained on workstation (AW4. 2). ROIs (5 mm × 5 mm) were placed in anterior funicalus, posterior funiculus, and bilateral lateral corticospinal tracts (LCTs), respectively, at the same slice (C3). Independent-sample t test was used for comparison of parameters between the two groups. Correlations between DTI parameters of ALS and ALS course, Norris score, and ALSFRS were carried out separately by Pearson correlation analysis. Results FA and RA values of bilateral LCTs were decreased significantly. FA/RA values of left LCT were 0.762±0.089 and 0.762±0.107 in ALS group, while they were 0.863±0.098 and 0.890±0.105 in control group, respectively. FA/RA values of right LCT were 0.751±0.065 and 0.772±0.082 in ALS group, and they were 0.843±0.118 and 0.863±0.134 in control group, respectively, they were decreased significantly (t = 2.575、4.195、2.246、2.218, P < 0.05). There were no significant differences (t = - 1.319, - 1.087, P > 0.05) between ADC values of left and right CSTs in ALS group [(0.744±0.162) × 10~(-3), (0.767±0.141) × 10-~(3) mm~2/s] and control group [(0.640±0.149) ×10~(-3), (0.643±0.168) ×10~(-3) mm~2/s)]. FA, RA and ADC values of ALS patients in anterior funiculus were 0.637±0.113, 0.622±0.138, (0.950±0.354)×10~(-3) mm~2/s, in control group they were 0.670±0.117, 0.656±0.136, (0.865±0.238) × 10~(-3) mm~2/s, there were no significant differences (t = 0.854, - 0.704, - 1.155,P > 0.05). FA, RA and ADC values of ALS patients in posterior funieulus were 0.886±0.073, 0.920±0.100, (0.613±0.137)×10~(-3) mm~2/s, in control group they were 0.906±0.078, 0.914±0.135, (0.636±0.224) × 10~(-3) mm~2/s, there were no significant differences (t = 1.655, - 0.148, - 1.360; P > 0.05). No significant correlation existed between FA and RA values and disease course, Norris and ALSFRS score (P > 0.05), in left and right LCTs. Conclusion DTI with SE-EPI technique is simple and sensitive to detect the pathological changes of the cervical spinal cord in ALS patients. DTI can reveal the abnormalities which are "normal appearing" on conventional T_2WI.