OBJECTIVE:To analyze clinical influencing factors and monitoring results of serum concentration of digoxin (SDC) to provide reference for rational use of drug. METHODS: 137 medical records were collected and analyzed statistically with multiple regression analysis in order to study the relationship of SDC with influencing factors. RESULTS: SDC was significantly affected by dosage, height, body weight, free fat body weight, body surface area, creatinining clearance rate, serum creatinine (P

Objective To investigate the CT features of desmoplastic small round cell tumor (DSRCT)occurred in abdomen and pelvis.Methods The CT images of 4 patients with pathologically proven DSRCT occurred in abdomen and pelvis were retrospectively analyzed.Results The CT features common to the 4 DSRCT in abdomen and pelvis were as follows:the lobulated soft-tissue masses were very large;the masses often involved omental and serosal surfaces of abdomen and pelvis organ and had close relationship to the surrounding organs;the originating site of the tumors were not apparent;intratumoral hemorrhage or necrosis were often present;and contrast-enhanced CT scan showed mild to medium heterogeneous enhancement.Conclusion The CT features of DSRCT in abdomen and pelvis are rather specific and can provide helpful information to the clinical diagnosis.

The molecular targeted therapy method using microRNAs(miRNAs)is gradually stepping into people′s vision. miRNAs affect colorectal cancer progress via abnormal expression in tumor cells or micro-environment. The high or low expressions of miRNAs in specific tissues probably have an impact on the expressions of oncogenes,tumor suppressor genes or other aspects including the surrounding environments,the metastases and the drug tolerance of tumors,thus contributing to curing the colorectal cancer. Nowadays, miRNA has entered the stage of animal experiments.

Objective To investigate the dose-response and its mechanisms of preventive effect of 103 Pd radioactive stent on restenosis in rabbits after balloon injury. Methods The abdominal arteries of the rabbits randomized into common or radioactive stent groups (including 5 Gy, 15 Gy, 25 Gy, 35 Gy groups) were balloon injured, and then stented. Intravascular ultrasound and angiography on abdominal arteries, immunohistochemical study of PCNA, and TUNEL assay were performed 8 weeks after stenting. Results Both the minimal lumen diameters and in-stent lumen areas in 103 Pd radioactive stent groups [(1.94?0.21) mm and (5.99?0.43) mm2, (2.17?0.12) mm and (7.01?0.93) mm2, (2.43?0.22) mm and (8.09?0.89) mm2, (2.56?0.27) mm and (8.49?0.54) mm2 in turn in 5 Gy, 15 Gy, 25 Gy, 35 Gy groups, respectively]were significantly improved with increases of the doses at the end of 8 weeks,revealing a dose-response effect. The positive ratio of PCNA in each 103 Pd stent group was reduced as compared with that in commen stent group (P

Age-related macular degeneration( AMD) is one of the major reasons of blindness among the elderly in the developed countries. As AMD patients are increasing year by year, AMD has become one of the important topics of ophthalmic research to prevent blindness. Its pathogenesis is not fully understood, but many studies have shown that vascular endothelial growth factor ( VEGF ) plays an important role in the pathogenesis. With the development and application of anti - VEGF drugs, there are a variety of drugs applied to the disease. This article introduces conbercept for the treatment of AMD.

Objective To explore the diagnostic value of serum miRNA-182 in colorectal cancer(CRC).Methods A total of 53 CRC patients were enrolled in this study,serum miRNA-182 was detected by quantitative real time polymerase chain reaction(qRT-PCR).The results were compared with those of patients with benign intestinal diseases and healthy controls.Results The content of serum miRNA-182 in CRC patients was significantly higher than those in patients with benign intestinal diseases and healthy group(P<0.05).The ROC curve showed that the area under the curve of serum miRNA-182 was 0.846,the confidence interval was 0.768-0.925,the diagnostic sensitivity was 77.1%,and the specificity was 81.1%.Serum miRNA-182 levels were correlated with TNM stage(P=0.011) and distant metastasis(P=0.002) in patients with CRC,but no significant correlation with other clinical parameters(P>0.05).Serum miRNA-182 level in CRC patients after surgery was significantly lower than that before operation(P<0.01),and which was significantly higher in patients with recurrence/metastasis after surgery(P=0.019).Conclusion Serum miRNA-182 level increased significantly in CRC patients,and which was closely related to the disease and malignant progression,serum miRNA-182 could be used as a potential marker for clinical diagnosis and disease evaluation of CRC.

Objective To evaluate non operative and simple operative managements of the calcaneal fractures. Methods 97 patients with calcaneal fractures were treated by non operative management or percutaneous reduction with wires or screw fixation from April 1997 to December 2001 in our department. They were followed up for more than 2 years. 64 patients were treated with non operation, 27 were fixed with wires and 6 with screws. Results 23 patients had excellent results, 67 good, 5 fair and 2 poor. The total good and excellent rate was 92.7%. 2 patients experienced secondary arthrodesis 1 year after injury. Conclusion Although the calcaneal fractures can be treated satisfactorily with non operative management or percutaneous reduction with wires or screws fixation, further prospective and comparative studies should be done in clinic to determine whether the calcaneal fractures should be treated with open reduction and fixation or with simple operative management.

Objective To investigate the incidence and the drug-sensitivity results of mycoplasma infection in genital tract from the gynecological outpatients,in order to provide a laboratory evidence for clinical rational use of drug.Methods The drug sensitiv-ity kit was used for isolation and culture of mycoplasma,reproductive tract secretions samples of 1 067 cases of gynecological pa-tients were collected to do mycoplasma identification and drug sensitivity test.Results In 1 067 samples,the positive rate of myco-plasma were 659 samples,including ureaplasma urealyticum(Uu)positive rate was 72.99%(481/659),mycoplasma hominis (Mh) positive rate was 3.79%(25/659),Uu and Mh positive rate accounted for 23.22%(1 53/659).The drug sensitivity results of 12 kinds of antimicrobial agents showed that single infection Uu was more sensitive to josamycin,doxycycline,minocycline,tetracy-cline,clarithromycin,azithromycin and roxithromycin.Single infection Mh was more sensitive to doxycycline,josamycin,minocycline and tetracycline.Mixed infection of Uu and Mh was more sensitive to josamycin,minocycline and doxycycline.Conclusion The main pathogen isolated from patients infected with plasma in our hospital is Uu,whether single Uu or Uu,Mh mixed infection,there are differences in the sensitivity of antimicrobial,clinicians should use drug rationally according to the conditions of patients and drug sensitivity results to reduce drug resistance rate.

Objective To investigate the effect of hyperoxia on the expressions of cysteinyl aspartate specific proteinase -3(Caspase -3)and proliferating cell nuclear antigen (PCNA)in bone marrow mesenchymal stem cells (BMSCs).Methods Primary BMSCs from SD rats were cultured in vitro,BMSCs grew to 70% -80% degrees of fu-sion and then were randomly divided into room air group and hyperoxia exposure group.Each group was divided into 5 sub -groups,and cultured for 0,3,6,1 2,and 24 h respectively.Morphology investigation with inverted phase contrast microscope was adopted.The expressions of Caspase -3 and PCNA protein levels were detected by Western blot. Results (1 )As time wenty by,compared with room air group,the gap between cells increased,some of the cells be-came circular,and cell detachment and floating appeared in the hyperoxia -exposure group (>1 2 h)compared with room air group.(2)As time went on,compared with the room air group,the expression levels of Caspase -3 protein in the hyperoxia -exposure group were increased,and the difference was significant after 6 hours of culture (0.27 ± 0.04 vs 0.1 4 ±0.02,t =5.03,P =0.007).(3)Compared with room air group,the PCNA levels of the hyperoxiaexpo-sure group(6 h)decreased,and the difference in PCNA protein expression levels was significant after 6 hours of culture (0.27 ±0.04 vs 0.38 ±0.04,t =3.37,P =0.028).Conclusions Hyperoxia exposure increases Caspase -3 expres-sion levels and decreases PCNA expression,which may affect the proliferation and apoptosis of BMSCs.

Objective To know the pollution situation by microcystin MC in Tai Lake in Wuxi. Methods The MC content in 12 water samples and 28 fish samples in the Tai Lake were determined by ELISA. Results The positive rates of MC in water samples and fish samples were 100%. The mean content of MC in water samples was higher in July 9 874.8 pg/ml than in November 142.8 pg/ml. The mean content of MC in the livers was 27.84 ng/g, in the muscle it was 3.97 ng/g. Conclusion The Tai Lake has been contaminated by microcystin and it can be detected in the fish living in the lake.