AIM: To establish the quality standard for Duhuo Jisheng Mixture (Radix Angelicae Pubescentis, Herba Taxilli, Cortex Fraxini, etc.) METHODS: Herbs in Duhuo Jisheng Mixture were identified by TLC, and osthol content was determined by TLCS. RESULTS: Rhizoma Chuanxiong; Radix Angelicae Sinensis; Radix Saposhnikoviae; Radix Gentianae Macrophyllae; Cortex Eucommiae; Herba Asari; Radix Paeoniae Alba could be determined. Osthol showed a good linear relationship at a range of 0.466 - 3.600 ?g,r= 0.999 2 . The average recovery was 95.4% . CONCLUSION:The methods are available with a reproducibility and can control the quality of this mixture.

AIM:To study the detection limits of aristolochic acid in Herba Asari preparation by HPLC. METHODS: Whether Asarum hetertropoides Fr.Schmidt ver.Mandshuricum(Maxim.) Kitag contained aristolochic acid A or not by HPLC and MS,and the difference in the reference substance and the sample solution was compared. RESULTS: Aristolochic acid A could not be found in A.hetertropoides Fr.Schmidt ver.Mandshuricum(Maxim.)Kitag by HPLC,but be found by MS. CONCLUSION:A.hetertropoides Fr.Schmidt ver.Mandshuricum (Maxim.)Kitag contained trace aristolochic acid,but it is difficult to measure aristolochic acid in Herba Asari by HPLC.The baseline noise of the sample solution can interfere with the detection limit of the trace compound compound

China ; epidemiology ; Disease Notification ; standards ; Epidemiologic Methods ; Humans ; Models, Statistical ; Occupational Diseases ; epidemiology ; prevention & control ; Occupational Health Services ; statistics & numerical data ; Registries ; Wounds and Injuries ; epidemiology ; etiology ; prevention & control

BACKGROUND: Lanceolata and astragalus can improve myocardial ischemia, which are widely used in clinics.OBJECTIVE: To compare the effects of extract of lanceolata and astragalus on electrocardiogram (ECG) of rats with experimental myocardial ischernia.DESIGN: Completely randomized and controlled study.SETTING: Pharmacological College of Harbin Commerce University.MATERIALS: The experiment was carried out at the Laboratory of Pharmacological College of Harbin Commerce University from September to December 2002. Totally 30 healthy adult Wistar rats were divided randomly into 3 groups: control group, lanceolata group and astragalus group with 10 in each group.METHODS: ① After anesthesia, lead ECG Ⅱ of rats was recorded.30 g/kg saline, 30 g/kg lanceolata extract and 30 g/kg astragalus extract [extract of lanceolata and astragalus (raw drug: 1 g/mL)] were injected into rats in the control group, lanceolata group and astragalus group respectively. After 1-3 minutes, 0.5 U/kg pituitrin was injected intravenously (for model of myocardial ischemia), and then saline was used to dilute from 0.1to 0.2 mL for 10-second injection. Changes of ECG after injection of pituitrin were recorded with LMA-2B Ⅱ physiological recorder (migration of T wave and ST section and change of heart rate). ② Differences of data were compared with t test.MAIN OUTCOME MEASURES: Height of T wave and Migrations of ST section and changes of heart rate of ECG of rats before and after medication.RESULTS: Data of totally 30 rats was entered the final analysis. ①Height of T wave: The height was higher after medication than that before medication in three groups (P ＜ 0.01), but that in astragalus group was obviously lower than that in lanceolata group [(1.63±3.81), (2.18±2.93) mm,P ＜ 0.01]. ② Heart rate: The rate was lower after medication than that of before medication in three groups (P ＜ 0.01), and that in astragalus group was obviously lower than that in lanceolata group [(212.21 ±5.01),(221.18±5.66) time/minute, P ＜ 0.01]. ③ Height of ST section: There were not significantly different in three groups (P ＞ 0.05), and the difference was not obvious before and after medication (P ＞ 0.05).CONCLUSION: ① Both astragalus and lanceolata can protect experimental myocardial ischemia induced by pituitrin. ② Effect of lanceolata on reduction of T-wave height is stronger than that of astragalus, but the effect of lanceolata on reduction of heart rate is weaker than that of astragalus.

Objective To assess the value of the multi-slice CT based OMENS classification in diagnosing pediatric hemifacial microsomia. Methods Eleven patients including 9 boys and 2 girls with suspicion of hemifacial microsomia underwent MSCT. Multi-planar reformation (MPR), maximum intensity projection (MIP) and volume-rendered image (VR) reconstruction were performed at the workstation. The malformations of the facial structures were analyzed respectively with the OMENS classification system.Results On CT reconstruction images, hemifacial microsomia in all 11 patients was demonstrated clearly.Nine patients were found to have unilateral lesion, including 6 on the left side and 3 on the right side. Two patients presented bilateral involvement with various severity degree. The OMENS classification system can be perfectly used to analyze the deformities. Respectively, the orbit and zygomatic arch deformity involved 8 sides in 8 cases, the mandible deformity involved 10 sides in 9 cases, the auricular deformity involved 13 sides in 11 cases, and the soft tissue deformity involved 12 sides in 11 cases. Conclusion MSCT based OMENS classification system can make accurate and comprehensive evaluation of the morphological changes in hemifacial microsomia, which provide strong imaging evidence to clinical evaluation.

Objective To explore the effect of patients with acute cerebral infarction and complication of acute gastric mucosal lesions (AGML) on their short-term prognoses.Methods Two hundred and sixteen patients with acute cerebral infarction admitted to the Department of Neurology in Tianjin Nankai Hospital from January to December 2014 were enrolled, and they were divided into the control group without AGML (167 cases) and observation group with AGML (49 cases) according to whether AGML occurred or not. The digestive tract was monitored in the two groups, and the relationships between the incidence of AGML and the location of infarction, stroke classification, as well as the anti-thrombosis treatments like thrombolysis, anti-coagulation and anti-platelet, etc. were analyzed; the changes in scores of the National Institutes of Health Stroke Scale (NIHSS) on admission, 7 days and 14 days after onset and 14-day mortality of two groups were compared.Results AGML occurred in 49 of 216 patients (22.69%); the 14-day mortality of the observation group was obviously higher than that of the control group [6.12% (3/48) vs. 1.80% (3/167),P < 0.05], the incidence of infarction located in cerebellum, brainstem, multiple cerebral lobes, etc. (low density shadow > 1/3 hemispheres) in the observation group was higher than that in the control group [cerebellum: 18.37% (9/49) vs. 4.19% (7/167); brainstem: 24.49% (12/49) vs. 8.98% (15/167), multiple lobes: 16.33% (8/49) vs. 2.99% (5/167), all P < 0.05]; the incidence of cardiac cerebral embolism (CE) was significantly higher than that in the control group [55.10% (27/49) vs. 12.57% (21/167),P < 0.05]. With the extension of disease course, the NIHSS score of the observation group was increased, while the score of the control group was gradually reduced, and the NIHSS scores of the observation group were obviously higher than those of control group on the 7th and 14th day after onset (7 days: 18.12±4.20 vs. 10.93±6.73, 14 days: 19.33±3.11 vs. 9.66±9.15, bothP < 0.05). The thrombolysis, argatroban anti-coagulation and anti-platelet incidence between the two groups after treatments was of no statistically significant difference (the incidence in control group was 4.79%, 47.31%, 47.90%, and it was 4.08%, 44.90%, and 48.98% in observation group, allP < 0.05). Conclusion The occurrence of AGML complication in patients with acute cerebral infarction is closely related to their short-term prognoses, and when the cerebral embolus is cardiac in origin or the infarction is located at multiple cerebral lobes, brain stem or cerebellum, the probability of the occurrence of AGML is relatively high, suggesting a poor outcome.

Humans ; Oncogenes ; Stomach Neoplasms ; genetics

As a new concept, the definition of translational medicine remains obscure. The translational medicine connects the bench to bedside, and its importance would be more remarkable. The development of gastrointestinal surgery reflects the idea of translational medicine. To carry out the translational study, the gastrointestinal surgeon must learn how to find subjects from clinical problems, how to collect complete information and tissues, how to collect complete information and tissues, how to collaborate with others from different fields and how to utilize all kinds of resources. By translational studies, gastrointestinal surgeons may further improve the survival of patients with gastrointestinal tumor.
Gastrointestinal Neoplasms ; Humans ; Translational Medical Research

Gastrointestinal Diseases ; physiopathology ; Gastrointestinal Motility ; Humans ; Kinetics ; Postoperative Complications

Objective: To study the floating and sustained release performance of Xiaojin Cinnabar Floated Sustained Release Capsules. Methods: HPMC was used to form the framwork of floated sustained release capsules containing Xiaojin Cinnabar. The content, floating time in vitro and releasing rate were studied.Results: The floating time in vitro was over 3 hours. The total released amount in 3 hours came to 60%. The conventional Xiaojin Cinnabar didn't have the floating performance. Conclusion: Xiaojin Cinnabar Floated Sustained Relegse Capsules is superior to the conventional Xiaojin cinnabar in the sustained release performance.