Animals ; Autonomic Nervous System ; physiology ; Humans ; Parasympathetic Nervous System ; physiology ; Sepsis ; etiology ; physiopathology ; Sympathetic Nervous System ; physiology

AIM:To evaluate the efficacy and safety of Sub-tenon's anesthesia for compound trabeculectomy with high intraocular pressure.
METHODS: Forty - six eyes ( 46 cases ) of primary glaucoma received compound trabeculectomy under Sub-tenon's anesthesia, whose preoperative intraocular pressure were higher than normal after 24 to 48h of combined medication. Both efficacy and complication of the anesthesia were studied.
RESULTS: One minute after anesthetic injection, all cases were able to achieve the effect of analgesia and eye brake. During the operation, 0 level of anesthesia effect included 35 eyes ( 76%) , 1 level of anesthesia effect included 10 eyes ( 22%) , 2 level of anesthesia effect included 1 eye(2%). Only 1 case of these patients needed to add the surface anesthetic once, and other cases were successfully operatedunder Sub-tenon's anesthesia. The total effective rate was 98%. No anesthesia complications occurred in all cases.
CONCLUSION: Sub - Tenon's anesthesia is safe, effective, simple and quick for compound trabeculectomy with high intraocular pressure.

Objective Diabetic macular edema( DME) is a common complication of diabetic patients.Laser photocoagula-tion therapy is effective in the treatment of DME despite of some patients with unsatisfactory curative effect or recurrence.This study was to explore efficacy and safety of aescin combined with laser photocoagulation in DME treatment. Methods A total of 102 pa-tients with diabetic macular edema in our hospital were randomly divided into control group(51 cases of 67 eyes) and combined treat-ment group (51 cases of 69 eyes).Aescin combined with laser photocoagulation were applied in observation group, and laser photoco-agulation was applied in control group.Observation was made on the changes of intraocular pressure, best corrected visual acuity( BV-CA), central macular thickness(CMT) and the leakage in macular area between the two groups before and after he treatment, along with detection on adverse reactions during the treatment. Results Com-pared with pre-treatment, there were no significant differences be-
tween the two groups in intraocular pressure at 1, 2, 3, 6 months after therapy(P>0.05), and compared with control group in the same time point, the intraocular pressure showed no change significantly in combined treatment group(P>0.05).Compared with con-trol group after therapy, the average value of BVCA in observation group showed significantly decreased at 2, 3, 6 months(Z=2.130, 1.932, 2.283;P=0.037, 0.028, 0.005) while the average value of CMT showed significant increase( Z=1.979, 2.315, 3.08;P=0.045, 0.031, 0.000).Compared with control group at the same time point, the number of patients who suffered from the leakage in macular area significantly decreased in combined treatment group at 2, 3, 6 months(χ2 =4.213, 5.074, 6.625; P=0.040, 0.024, 0.010) .7 patients(13.7%) in combined treatment group suffered from stomach and nausea during the treatment, without any observation of serious adverse reaction. Conclusion Conclusion Aescin combined with laser photocoagulation is effective for the rehabilitation of patients and chconsolidate effect with high safety, which is of great clinical application.

Visualized concept map can develop the ability of self-learning ability, knowledge integration ability and teaming and collaboration ability in medical students. Concept map is also a useful tool for improving student learning and clinical reasoning. Application of concept map in inte-grated problem based learning curriculum of basic medical sciences may promote meaningful learning and enhance the efficacy of PBL.

0.05).The gene frequencies of ACG-T235M and ACE-DD plus AGT-TT were significantly higher in the patients with CAD than in the controls respectively(all P

Objective To evaluate application of fist clenching method with shaking hands in venipuncture to elderly angular patients. Methods 360 elderly angular patients were randomly divided into group A and group B with 180 cases in each group. Group A used fist clenching method with shaking hands, group B adopted traditional fist clenching method in venipuncture. Pain degree and one-time success rate of two groups were compared. Results One-time success rate of group A in venipuncture was obviously higher than group B, while pain degree of group A was less than group B. Conclusions Fist clenching method with shaking hands to elderly angular patients can raise success rate in venipuncture, and reduce pain degree of patients.

AIM: To investigate the potential causes and management of the clusters of diffuse lamellar keratitis (DLK) after laser corneal refractive surgery.METHODS: The study enrolled 98 eyes (53 patients) complicated with DLK after receiving laser in situ keratomileusis (LASIK), FS-LASIK or small-incision lenticule extraction (SMILE) in our center from February 10th,2016 to February 22th,2016.They were given clinical classification treatments according to corneal layer inflammatory extent and then followed up after 1, 3, 5, 7, 10d and 1mo.RESULTS: The clusters of DLK occurred 5 times in the study period.The incidence and degree of DLK significantly decreased after changed the sterilization, surgical equipments, temperature and humidity of the operating room.There were 80 eyes (82%) had stage 1 DLK, 11 eyes (11%) had stage 2, 4 eyes (4%) had stage 3 and 3 eyes (3%) had stage 4.The incidence of DLK after FS-LASIK was 40% (79 eyes in 42 patients), that after LASIK assistant by Hastome keratome was 45% (10 eyes in 5 patients), that after SMILE was 20% (9 eyes in 6 patients).After intensive treatment, as glucocorticoid treatment and flap lifting flushing, all cases recovered within 1mo.CONCLUSION: The outbreak of DLK may be associated with the disposable item, flushing liquor, temperature and humidity of the operating room.Early diagnosis, prevention and treatment are the key of decreasing the incidence of DLK.

The requirements for library-provided knowledge service in training of doctorial students were studied according to the analysis of problems in trainingof doctorial students .Theinformation support platform for innova-tive training of doctorial students was constructed by incorporating knowledge service intothe training of doctorial students from the aspects of topic selection, project establishment, scientific research,project completion and choosing excellent doctorial students in order to improve their information awareness and innovative ability.

BACKGROUND:According to the previous studies, it is known that bone marrow mesenchymal stem cells live in the physiological environment of lower oxygen concentration compared with the common culture concentration (20%-21%). Hypoxia can promote bone marrow mesenchymal stem cells proliferation and maintain the characteristics of differentiation potential.
OBJECTIVE:To understand the biological characteristics of bone marrow mesenchymal stem cells after hypoxic preconditioning, and search for in vitro basis for bone marrow mesenchymal stem cells therapy in vivo with hypoxic preconditioning strategy.
METHODS:After passage and inoculation, bone marrow mesenchymal stem cells were cultured in the hypoxic incubator (1%O2, 5%CO2). Bone marrow mesenchymal stem cells cultured in the normoxic incubator (20%O2, 5%CO2) served as controls.
RESULTS AND CONCLUSION:Compared with bone marrow mesenchymal stem cells cultured under normoxia (20%O2), bone marrow mesenchymal stem cells cultured under hypoxia (1%O2) began to display exponential growth phase after a longer incubation period. Hypoxia (1%O2) was not the lethal condition for bone marrow mesenchymal stem cells. cellsurvival rates of both groups reduced over time, while those of bone marrow mesenchymal stem cells cultured under normoxia reduced faster (P<0.05). Surface markers expression of bone marrow mesenchymal stem cells, including CD29(+), CD90(+), CD31(-), CD45(-), after hypoxic preconditioning did not change greatly when compared with the results before hypoxic preconditioning. The hypoxia preconditioned bone marrow mesenchymal stem cells did not differentiate into vascular endothelial cells. Hypoxia-inducible factor-1αprotein expression was promoted after 24 hours to 48 hours hypoxic preconditioning, and there was significant difference between the two time points (P<0.05). Vascular endothelial growth factor expression increased at both transcriptional and protein levels after 48 hours hypoxic preconditioning (P<0.05). Culture under hypoxia (1%O2) does not produce a lethal effect on bone marrow mesenchymal stem cells and can be used for hypoxic preconditioning. Surface markers of bone marrow mesenchymal stem cells after 48 hours hypoxic preconditioning exhibit no great changes and the hypoxia preconditioned bone marrow mesenchymal stem cells maintain the characteristics of stemness. Hypoxic preconditioning can promote the ability of bone marrow mesenchymal stem cells to survive under hypoxia/ischemic condition. The activation of the hypoxic response signal transduction pathway through hypoxia-inducible factor-1αmay explain this.

Animals ; Antibodies, Anti-Idiotypic ; therapeutic use ; Disease Models, Animal ; Doxorubicin ; toxicity ; Immunotherapy ; Interleukin-18 ; immunology ; pharmacology ; Male ; Mice ; Nephrosis, Lipoid ; chemically induced ; pathology ; therapy