Adult ; Chromosome Aberrations ; Female ; Humans ; Infant, Newborn ; Karyotyping ; Male ; Pedigree

To investigate the pathogenic mechanism of interleukin 6(IL-6) in the tubulointerstitial lesions of patients with active lupus nephritis(LN). Methods Uninary IL-6 from 42 active LN cases and the renal tissues sections from 15 of them were examined by performing ELISA and in situ hybridization with IL-6 cDNA probe labelled by digoxingenin respectively. Results In 36/42 (85.7%) of active LN patients, uninary IL-6 content was higher than or equal to 5 pg/mg ,cr, while no one case did in normal controls. Uninary IL-6 content was highly correlated with the uninary ft-m and NAG excretion respectively. In normal tubules and interstitium, IL-6 mRNA was undetectable. For all sections from 15 active cases, IL-6 mRNA was found in tubular and interstitial cells, and the distribution correlated with the extent of tubulointerstitial lesions. Conclusion IL-6 is probably involved in tubulointerstitial lesions of patients with active lupus nephritis.

The relationship of the nose tip position and the skull figures which were measured from the lateral craniographs in 49 ladies of 17～19 age living in Shenyang was studied. The figures measured on craniographs were analyzed by multiple stepwise regression analysis on IBM-PC/XT computer, and the regressoin equations for estimating the nose tip position of female in Shenyang were obtained. Cases in which the errors were within 2 mm were 71.43 to 77. 55 percent.

Objective To investigate the influence of mental intervention on the self-image of breast cancer patients.Methods Fifty patients with breast cancer who were hospitalized in our hospital from January to September,2015 were enrolled as the control group in the study,where routine nursing intervention,health education on disease related knowledge and psychological nursing were carried out.During October 2015 to June 2016,50 patients with breast cancer were enrolled as the observation group,where psychological awareness intervention was carried out apart from the routine nursing intervention.The self-image level of breast cancer patients from the two groups was evaluated by self-image questionnaire for breast cancer patients (BIBCQ).Result After intervention,the scores on self-image in the observation group were significantly higher than those in the control group (P＜0.05).Conclusion Mental intervention can improve the self-image of breast cancer patients and help improve their quality of life.

In addition to kill tumor cells through direct cytotoxicity, the immunomodulatory activity is another facet of chemotherapy. The major molecular mechanisms of immunogenicity of cytotoxic drugs are elimination of CD_4~+ CD_(25)~+ regulatory T cells or Myeloid-Derived Suppressor Cells, and sensitization of tumor cells to immune effectors through modulation of death receptor expression. These studies provide scientific evidence for combinatorial treatments including vaccines and Traditional Chinese Medicine for cancer therapy.

Objective To explore the changes of the peripheral blood soluble Fas (sFas) and soluble FasL (sFasL) in patients of diabetes complicated with tuberculosis,in order to provide the basis for condition judgment and intervention.Methods The patients of diabetes complicated with tuberculosis (diabetes comphcated with tuberculosis group,25 cases),simple diabetes (diabetes group,25 cases),simple tuberculosis (tuberculosis group,25 cases) and healthy person (control group,25 cases) were selected.The peripheral blood sFas,sFasL and T-lymphocyte subsets were examined by enzyme-linked immunosorbent test.Results The peripheral blood sFas,sFasL in diabetes complicated with tuberculosis group,diabetes group,tuberculosis group was higher than that in control group[(7.91 ± 1.93),(8.74 ± 2.12),(7.86 ± 1.61)mg/L vs.(2.10 ±0.88) mg/L and (562.37 ± 196.38),(1512.32 ±303.48),(607.48 ± 102.53) ng/L vs.(263.18 ±46.32) ng/L](P＜ 0.05).The peripheral blood sFas,sFasL in diabetes group was higher than that in diabetes complicated with tuberculosis group and tuberculosis group (P ＜ 0.05).With sFasL 1000 ng/L as boundary value,the diagnostic coincidence rate of diabetes complicated with tuberculosis group and diabetes group was 90.00％ (45/50).CD3 +,CD4+ T-lymphocyte subsets in diabetes complicated with tuberculosis group,diabetes group,tuberculosis group was lower than that in control group (0.3376 ± 0.0712,0.2368 ± 0.0803,0.4801 ± 0.0896 vs.0.5849 ± 0.0487 and 0.1798 ± 0.0401,0.2100 ± 0.0679,0.2312 ± 0.0487 vs.0.2811 ± 0.0348) (P ＜ 0.05).CD3 + T-lymphocyte subsets in diabetes complicated with tuberculosis group was higher than that in diabetes group and lower than that in tuberculosis group (P ＜ 0.05).CD4+ T-lymphocyte subsets in diabetes complicated with tuberculosis group was lower than that in diabetes group,tuberculosis group(P ＜ 0.05).CD8+ T-lymphocyte subsets in diabetes complicated with tuberculosis group,diabetes group was higher than that in control group (0.3209 ± 0.0707,0.2831 ± 0.0794 vs.0.2086 ± 0.0589)(P ＜ 0.05).CD8+ T-lymphocyte subsets in diabetes complicated with tuberculosis group was higher than that in diabetes group and tuberculosis group (0.2287 ± 0.0690)(P ＜ 0.05).C D3 +,CD4+,CD8+ T-lymphocyte apoptotic rate in diabetes complicated with tuberculosis group,diabetes group,tuberculosis group was higher than that in control group [(4.34 ± 2.08)％,(3.22 ± 2.12)％,(2.59 ± 1.41)％ vs.(1.01 ± 0.38)％,(5.12 ± 1.58)％,(4.82 ± 1.98)％,(3.21 ± 1.19)％ vs.(1.78 ±0.53)％ and (1.45 ±0.52)％,(2.31 ±2.01)％,(1.62 ± 1.33)％ vs.(1.07 ± 0.38)％] (P ＜ 0.05).CD3 +,CD4+ T-lymphocyte apoptotic rate in diabetes complicated with tuberculosis group,diabetes group was higher than that in tuberculosis group (P ＜0.05).CD8+ Tlymphocyte apoptotic rate in diabetes group was higher than that in tuberculosis group (P ＜ 0.05).Conclusions The peripheral blood sFas and sFasL exists abnormal increase in patients of diabetes complicated with tuberculosis.CD3+,CD4+ T-lymphocyte decreasing shows that the patients exist immune function disorder.sFas and sFasL may be involved development process of disease,and the peripheral blood sFasL content can also be used as auxiliary indicators for identifying diabetes patients with tuberculosis.

Objective To evaluate the diagnostic value of thinprep cytologic test (TCT) combined with high-risk human papillomavirus (HR-HPV) DNA for cervical cancer (CC). Methods 141 cases of patients with abnormal cervical lesions were abnormal examined by TCT and were graded by the results of TCT and cervical biopsy, the HR-HPV-DNA was detected by surface plasmon resonance (SPR). Results The cervical biopsy positive rate 65.2% (92/141) was significantly higher than the positive rate of TCT 39.0% (55/141) (χ2=19.45, P < 0.05). The positive rate of HR-HPV-DNA was 66.0% (93/141) was significantly higher than the positive rate of TCT 39.0% (55/141), (χ2=20.53, P < 0.05). Conclusion TCT, HR-HPV-DNA and cervical biopsy are important clinical diagnostic methods for cervical lesions, combine detection of TCT and HR-HPV-DNA can improve the detection rate of cervical lesions.

Objective To study the effect of external application of Wuwei Shuangbai Powder on relieving primary liver cancer pain and to observe its effect on hemorheology indexes.Methods A double-blind,randomized and controlled trial was carried out in 60 primary hepatocellular carcinoma patients with cancer pain.The patients were divided into control group and treatment group,30 cases in each group.The treatment group was given external application of Wuwei Shuangbai Powder and oral use of placebo capsules,and the control group was given external application of placebo powder and oral use of celecoxib capsules.The treatment for the two groups lasted for 7 days.Before and after treatment,pain was scored by Numeric Rating Scale (NRS),and the peripheral blood hemorheology indexes of both groups were observed.Results (1) After treatment,the NRS pain scores of both groups were decreased compared with those before treatment (P ＜ 0.05 or P ＜ 0.01),and the scores of the treatment group were significantly lower than those of the control group (P ＜ 0.05),indicating that the treatment group had better effect on relieving cancer pain.(2) After treatment,the indexes of hemorheology of treatment group were significantly higher than those of the control group,and the differences of the indexes except the hematocrit were significant between the two groups(P ＜ 0.05 or P ＜ 0.01).Conclusion Wuwei Shuangbai Powder is effective for the treatment of primary liver cancer pain.The mechanism may be related to the improvement of hemorheology of liver cancer patients.

Objective: To determinate the content of isoimperatorin in Exocarpium citri grandis from 10 various species and origins. Methods: The isoimperatorin was determinated at 305nm by TLC Scanning. Results: The content of isoimperatorin in Exocarpium citri grandis was very low, and the law of distribution could not be found. Conclusion: Although the content of isoimperatorin in Exocarpium citri grandis was very low, the method is very sensitive and simple and the result is accurate.

Object To establish the HPLC fingerprint of flavonoids in Exocarpium Citri Grandis (ECG) Methods HPLC fingerprint of ten samples were established. The naringin was used as standard. The relative migration time and the percentage of peak area were used as parameters Results More than 30 peaks occurred on the HPLC fingerprint of flavonoids of ECG. The fingerprints of C. grandis ‘Tomentosa’ and C. grands had obivous differences Conclusion The method is simple and accurate with a good reproducibility. It could be used for quality control and identification of ECG