1.Advances in research of volatile oil and its different chemotypes in leaves of Perilla frutescens.
China Journal of Chinese Materia Medica 2015;40(15):2937-2944
The volatile oil is the main component in the leaves of Perilla frutescens. According to the main types of monoterpenoids or aromatic compounds, it can be divided into different chemotypes and the main chemotypes of Chinese producing Perilla are PA type (mainly containing Perilla aldehyde and limonene), PK type (mainly containing perillaketone) and PP type (subdivided as PP-a type, with apiole as its main component; PP-m type, with myristicin as its main component; PP-e type, with elemicin as main component; PP-as type, with asarone as main component). Based on the biosynthetic pathways analysis, we also found that the formation of the particular chemotype is usually controlled by a single gene or a few genes, and different types have different pharmacological effects. In this paper, the classification under the species P. frutescens, main chemotypes of the volatile oil, and their biogenesis and regulation, pharmacological effect and influence factors are summarized and reviewed.
Animals
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Humans
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Oils, Volatile
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analysis
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pharmacology
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toxicity
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Perilla frutescens
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chemistry
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classification
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metabolism
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Plant Leaves
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chemistry
2.Effect of 17β-estradiol on idiopathic ventricular arrhythmia and IP3/Ryn 2 expression of receptor-regulated calcium pool during peri-menopausal period
Ling TONG ; Xianjiang WEI ; Chunxiang LIN
Chinese Journal of Biochemical Pharmaceutics 2017;37(1):145-146,150
Objective To observe the curative effect of 17β-estradiol on idiopathic ventricular arrhythmia (IVA) during peri-menopausal period and explore the association with IP3/Ryn expression of receptor-regulated calcium pool. Methods 32 cases IVA during peri-menopausal period (IVA group) and 32 cases healthy women during peri-menopausal period of the same levels of age (normal control group) were selected. The IVA group received oral 17β-estradiol for 2 months. The count of arrhythmia in IVA group was recorded, the IP3/Ryn 2 expression pre- and post-treatment in IVA group and normal control group were detected. Results Compared with pre-treatment, 17β-estradiol significantly reduced the occurrence of IVA (P<0.05). Compared with pre-treatment, 17β-estradiol significantly reduced IP3 expression(P<0.05), while there was no significant difference compared with normal control group. 17β-estradiol had no significant effect on Ryn 2 expression. Conclusion The anti-IVA effect of 17β-estradiol may be associated with the IP3 receptor gene expression.
3.A novel citrinin derivative from the marine-source fungus Penicillium citrinum.
Min NI ; Wei-Ling LIN ; Peng YANG ; Shui-Chun MAO
Acta Pharmaceutica Sinica 2015;50(2):203-206
A novel citrinin derivative, penicitrinol L (1), along with two known analogues, penidicitrinin B (2) and pennicitrinone A (3) were isolated from the marine-source fungus Penicillium citrinum. The structure of the new compound was elucidated by spectroscopic methods including one and two-dimensional NMR as well as high-resolution mass spectrometric analysis. Furthermore, compound 1 showed modest cytotoxic activity against HL-60 cell line and compound 3 showed weak cytotoxic activity against A375 cell line.
Antineoplastic Agents
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chemistry
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isolation & purification
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Citrinin
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analogs & derivatives
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chemistry
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isolation & purification
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HL-60 Cells
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Humans
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Magnetic Resonance Spectroscopy
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Penicillium
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chemistry
4.The effects of safety organizing on quality of nurse work life in PICU
Minli LING ; Xiaomin LIN ; Lingli ZHANG ; Wei XU ; Chengcheng SU
Chinese Journal of Primary Medicine and Pharmacy 2017;24(4):499-502
Objective To understand the present situation of safety organizing and quality of nurse work life in pediatric intensive care unit(PICU),and to discuss the relationship between safety organizing and quality of nurse work life.Methods A total of 279 PICU nurses in eight hospitals in Zhejiang were selected by the safety organizing scale (SOS)and quality of nursing work life(QNWL).Results The score of SOS was (45.23 ±10.88)points,and the score of QNWL was (159.46 ±33.60)points.There was a positive correlation between safety organizing and quality of nurse work life(r =0.256,P <0.05).Conclusion The nurse managers should improve the level of quality of nurse work life by safety organizing.
5.Effects of all-trans retinoic acid on proliferation,iodide uptake and thyroid specific gene expression in different cell lines of human thyroid carcinoma
Dai CUI ; Ling LAN ; Chao LIU ; Lin JIANG ; Wei TANG ;
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
Different human thyroid carcinoma cell lines were treated with all-trans retinoic acid(RA). RA could inhibit cell growth,improve iodide uptake and increase some thyroid specific genes and retinoid acid receptor(RAR)mRNA expressions in FTC-133 cells.However,RA had no effect in C643,HTH74 and XTC. UC1 cells.These findings indicate that different thyroid carcinoma cells display diverse responses to RA.
6.Association of CA repeats polymorphism in the promoter region of the insulin-like growth factor- Ⅰ gene with type 2 diabetes
Min YANG ; Wei LING ; Jing LIN ; Changlin NI
Chinese Journal of Endocrinology and Metabolism 2010;26(12):1042-1044
Objective To investigate the association of CA repeats polymorphism in the promoter region of human IGF-I gene with type 2 diabetes in narthen Han nationality. Methods Gender-age matched subjects with type 2 diabetes and normal glucose tolerance (NGT) were enrolled for this study, 147 subjects in type 2 diabetes group and 159 subjects in NGT group. Genomic DNA was extracted by standard methods. PCR, Genescan,Genotyper, and direct sequencing were conducted to screen CA repeats polymorphism in the promoter region of the human IGF-I gene. Results No significant association was observed between any ( CA), repeat genotype and type 2 diabetes. A lowered serum uric acid was seen in genotypes that included alleles with larger than (CA) 20 repeats [(4.18 ± 1.25 vs 4.63 ± 1.36) mg/dl, P = 0.03]. Conclusion Alleles with larger than (CA) 20 repeats may be a protective factor for type 2 diabetes.
7.Effect of madacassoside on D-galactose-induced learning and memory impairment in mice
Shimei TAN ; Ling WEI ; Quanfang HUANG ; Xing LIN
Chinese Pharmacological Bulletin 2015;(9):1239-1243,1244
Aim To investigate the protective effect and mechanism of madecassoside from hydrocotyle sibthorpioides (MHS)on learning and memory impair-ment induced by D-galactose (D-gal)in mice.Meth-ods Totally 75 SPF Kunming male mice were divided into normal control group,model group,low-,middle-and high-doses of MHS treated groups.The dementia models were induced with D-gal.The learning and memory functions were tested by Morris water maze, the level of Aβ1 -42 and its related proteins in the hip-pocampus was determined by Western blot,and the ex-pression of Aβ-related genes were determined by RT-PCR.Results Compared with model group,MHS markedly decreased the content of Aβ1 -42 ,inhibited the expression of APP,BACE1 and CatB,but promo-ted the expression of NEP and IDE.In addition,AHS significantly increased the expression of plasticity-relat-ed proteins including PSD-95,p-NMDAR1,p-CaMKII, p-PKACβ,PKCγ,p-CREB and BDNF.Conclusions MHS could remarkably ameliorate the learning and memory impairment induced by D-gal in mice,which may be due to its ability to inhibit the Aβgeneration and deposition and promote synaptic plasticity related protein expression.
8.Magical tools of detection and processing of medical information in Traditional Chinese Medicine
Gang LI ; Wei FANG ; Jing ZHAO ; Ling LIN
International Journal of Biomedical Engineering 2016;39(1):-
The progress of objective and digital diagnosis of Traditional Chinese Medicine (TCM) is not optimistic.There is no denying that there are a lot of research ideas and methods,on the other hand,the understanding of TCM is very one-sided,fuzzy and even wrong.This paper attempts to absorb the quintessence of TCM in clinical medical information detection and treatment ideas from the measurement science and modem information detection and processing point of view.Ideas and means of clinical medical information detection and treatment in TCM not only coincide with,but also improve that of modem information detection and processing way and means,which have high reference value.The way of thinking and means,not only for medical information detection and processing,but also provide a valuable reference for other areas of information detection and processing.
9.Exploration on the construction of experimental teaching demonstration centre for preventive medicine
Hua CHEN ; Wenchang ZHNAG ; Ling ZHENG ; Jiali WANG ; Wei LIN
Chinese Journal of Medical Education Research 2005;0(06):-
The reform and practice of Preventive Medicine Experiment Center of Fujian Medical University are introduced in this paper. How to construct a preventive medicine experiment center to adapt to modern medicine mode was explored in the aspects of experimental teaching concept,teaching system,teaching team and management system.
10.Study on biological basis of phlegm and blood stasis syndromes in TCM
Ling ZHAO ; Haifeng WEI ; Li ZHANG ; Yali LI ; Lin LI
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(08):-
Objective:To investigate the biological basis of phlegm syndrome (Tan Zheng) and blood stasis syndrome (Xue-Yu Zheng) in traditional Chinese medicine(TCM) through the dynamic observation of the changes of blood lipid hemorheology and vessel endothelium impairment. Methods: SD rats were divided into fi ve groups randomly: the control group fed by normal diet, four model groups fed by 2-, 4-, 8- and 16-week high cholesterol diet, respectively. The indices of blood lipid hemorheology the level of plasma endothelin (ET) and serum nitric oxide (NO) were detected. Results: Compared with normal control group, serum total cholesterol (TC) and LDL-C levels raised signif icantly after being given hypercholesterol diet for 2 weeks, and this changes lasted till 16 weeks(P