Sudden cardiac arrest is one of the critical clinical syndromes in emergency situations. A cardiopulmonary resuscitation (CPR) is a necessary curing means for those patients with sudden cardiac arrest. In order to simulate effectively the hemodynamic effects of human under AEI-CPR, which is active compression-decompression CPR coupled with enhanced external counter-pulsation and inspiratory impedance threshold valve, and research physiological parameters of each part of lower limbs in more detail, a CPR simulation model established by Babbs was refined. The part of lower limbs was divided into iliac, thigh and calf, which had 15 physiological parameters. Then, these 15 physiological parameters based on genetic algorithm were optimized, and ideal simulation results were obtained finally.
Algorithms ; Cardiopulmonary Resuscitation ; Death, Sudden, Cardiac ; Electric Impedance ; Hemodynamics ; Humans ; Leg ; Models, Cardiovascular

· Mesenchymal stem cells ( MSCs ) are a population of multipotent stem cells with various neurotrophins from bone marrow which are widely used in tissue, cell repair and alternative research.Recently, some researches have shown that MSCs could enhance the viability of neurons under a pathological circumstance by secreting some neurotrophins. So the neuroprotection of MSCs can provide a new method of the treatment on retinopathy that it possible to promote cells survial and functional recovery.Here we make a brief review on the secretory function of neurotrophin and neuroprotective effects of MSCs on retinal cells and its application in the treatment of retinal disease.

Humans ; Immunohistochemistry ; Lymphoma, Large B-Cell, Diffuse ; classification ; pathology

Objective: To explore the gender difference of defensive style in Chinese college students. Method; Meta-analytic method was used to calculate 15 group data in 13 studies from 1990 to 2009, to test whether there were differences between male and female college students in three defensive styles and image distorting factor. Results: The d-values of immature defense style, mature defense style, and intermediate/neurotic defense style were -0.08 (95% CI : -0.12～ -0.04), -0.01 (95% CI: -0.05～ -0.03), and -0.05(95% CI: -0.09～-0.01) respectively. And the d - value of image distorting factor was 0.07 (95% CI: -0.02 ～0.15) . Conclusion: There is no gender difference in the defensive styles in Chinese college students.

With the development of the thoracic surgery, clinicians are paying more and more attention to acute lung injury (ALI) following thoracic surgical procedures. Many factors may cause ALI during the operation. This review updates recent researches on the pathogenesis and treatment strategies of the ALI in thoracic surgical procedures.

Objective To investigate the 6 short tandem repeats(D2S1338,D8S1179,D16S539,D18S51,D19S433,D21S11) polymorphism in Maonan and Mulao minority and their genetic relationships with other 5 minorities in Guangxi Province and to enrich the genetic database of Maonan and Mulao populations. Methods The allelic frequencies and the genotype of six STR loci were generated from 383 unrelated Maonan and Mulao individuals by PCR_STR and ABI3100. Results Sixty_one and 65 STR alleles were found in the six STR loci of Maonan and Mulao populations,with their frequencies ranging from 0.002 5-0.320 0 and 0.002 7-0.284 2 respectively.Two hundred and sixteen and 218 genotypes were found in Maonan and Mulao populations,with their frequencies ranging from 0.005 0-0.150 0 and 0.005 5-0.158 5 respectively.Their average heterozygosities were above 0.8;polymorphism information content were above 0.8 their accumulative discrimination power were over 0.999 999 98,and the probability of paternity exclusion were over 0.998 3.The results of genetic distance and phylogenetic tree showed that there were closest genetic relationship between Maonan and Mulao minorities,and farthest genetic relationship was existed between Shui and Yi minorities.Seven minorities were clustered to 2 groups on phylogenetic tree.Yi minority was clustered to one group,whereas the other 6 minorities(Maonan,Mulao,Hui,Miao,Shu and Jing nationalities) were clustered to the other group.Conclusion Six STR loci of Maonan and Mulao minorities possesse the characteristic of high genetic diversities which has great practical value.Therefore,the obtained data can be used in human population genetics investigation,individual indentification and paternity test.The genetic variation of STR among 7 populations is basically consistent with their historical culture and geographic distribution.

Objective To investigate the clinical efficacy of CRRT in the treatment of severe pancreatitis.Methods 120 patients with severe pancreatitis were divided into two groups according to the treatment.All the patients were given basic treatment in all groups.The Pa arterial oxygen pressure (PaO2),oxygenation index (PaO2/FiO2),and C-reactive protein (CRP) in the two groups after 72 hours of treatment were observed.The levels of blood lactate (Lac),APACHEII,tumor necrosis factor α(TNF-α),interleukin-6 (IL-6) and interleukin-1β (IL-1β) were measured before and after treatment 72 hours.A comparison of mortality and hospitalization time was observed in 28 days of treatment.Results After treatment,Lac [the control group (3.32±0.85)mmol/L,the observation group (2.55±0.65)mmol/L],APACHEII score [the control group (13.30±2.80)points,the observation group (12.01±2.60)points],CRP [the control group (24.30±2.80)mg/L,the observation group (12.33±1.60)mg/L] were significantly lower than before treatment [Lac:the control group (4.85±1.05)mmol/L,the observation group (4.90±1.02)mmol/L;APACHEII score:the control group (16.62±2.95)points,the observation group (16.90±3.01)points;CRP:the control group (40.32±3.10)mg/L,the observation group (40.40±3.51)mg/L;the control group:tLac=1.67,P=0.004,tAPACHEII=6.32,P=0.000,tCRP=29.71,P=0.000;the observation group:tLac=15.05,P=0.005,tAPACHEII=9.52,P=0.000,tCRP=56.36,P=0.000].PaO2 [the control group (75.30±4.80)mmHg,the observation group (84.31±4.60)mmHg], PaO2/FiO2 [the control group (225.30±14.83)mmHg,the observation group (256.31±14.65)mmHg] were significantly higher than before treatment [PaO2:the control group (60.32±4.15)mmHg,the observation group (60.40±4.01)mmHg;PaO2/FiO2:the control group (130.39±11.15)mmHg,the observation group (130.90±11.01)mmHg;the control group:tPaO2=18.29,P=0.000,tPaO2/FiO2=39.62,P=0.000;the observation group:tPaO2=30.35,P=0.000,tPaO2/FiO2=53.01,P=0.000].Those in the observation group were significantly improved than the control group (tLac=5.574,P=0.00,tAPACHEII score=2.615,P=0.005,tPaO2=3.646,P=0.0002,tPaO2/FiO2=11.523,P=0.00,tCRP=28.751,P=0.000).After treatment,the TNF-α,IL-6 and IL-1β levels in the two groups [the control group:IL-1β (70.32±6.85)ng/mL,IL-6 (103.30±8.80)ng/mL,TNF-α (89.30±8.80) ng/mL;the observation group:IL-1β(48.55±6.62)ng/mL,IL-6(92.01±8.60)ng/mL,TNF-α(57.31±7.60)ng/mL] were significantly improved than before treatment [the control group:IL-1β(82.85±7.05)ng/mL,IL-6(173.62±9.95)ng/mL,TNF-α (105.32±9.15)ng/mL;the observation group:IL-1β(83.90±7.32)ng/mL,IL-6 (175.90±10.01)ng/mL,TNF-α (106.40±9.01)ng/mL;the control group:tIL-1β=9.66,P=0.000,tIL-6=41.01,P=0.000,tTNF-α=9.77,P=0.000;the observation group:tIL-1β=27.74,P=0.000,tIL-6=49.23,P=0.000,tTNF-α=32.26,P=0.000].And those of the observation group improved more significantly than the control group (tIL-1β=17.702,P=0.00,tIL-6=7.107,P=0.00,tTNF-α=21.311,P=0.000).The mortality rate and hospitalization time of the observation group were 18.33% and (10.97±2.92)days,which were significantly lower than those of the control group [36.67%,(13.63±3.26)days;χ2=5.058,P=0.025;t=4.708,P=0.000).Conclusion The use of CRRT in the treatment of severe pancreatitis can improve the vital signs,reduce the inflammation index,improve the serum levels of inflammatory factors and lactic acid,reduce the mortality and hospital stay.

Objective Three kinds of different internal promoters were inserted into the lentiviral vector to drive the expression of CD19-specifc chimeric antigen receptor (CAR)-redirected T lymphocytes with the green fluorescent protein(GFP) and the GFP expression efficiency of transduced cells driven by lentivirus were compared.Methods Three GFP reporter lentivirus vectors carrying different promoters and anti-CD19-CAR,including CMV,EF1α,and CMV-EF1α were selected.Three different vectors' names abbreviate to pHAGE-CMV-EFlα-EGFP-CD19,pHAGE-CMV-EGFP-CD19,pHAGE-EF1α-EGFP-CD19.Human embroic kideny 293T cells were cotransfected with the three plasmids by calcium phosphate DNA precipation.And the expression of GFP was observed under fluorescent microscope after transfecting 293T cells,and virus supernatant was collected after 72 h and centrifuged.Nucleic acid copy number in RNA and the expression of EGFP and CD3 zeta were identified through fluorescence quantitative PCR,flow cytometry and western blotting,respectively.The titers of the lentiviral vectors were determinde by scoring GFP expression following swerial dilutions of the viral supernatant on 293T cells.T lymphocytes was infected with lentivims produced from these reporter vectors.Then,fluorescence microscope,wesyern blotting were used to detect the GFP expression strength.Results Results of fluorescence microscope maintained that different internal promotors driving the expressing effect is different.293T as targeted cell,the transfected 293T cells were found containing strong expression of GFP.The amount of the 293T cells expressing GFP driven by CMV-EF1α promoter was the largest,while the EF1α promoter was the smallest.Flow cytometry results show that the rate of transduction is 72.4％,20.6％ and 14.5％,respectively.T lymphocytes as targeted cell,Western blot showed that CMV-EF1α was the largest among all promoters,while the CMV was the smallest.Conclusion In the study of gene expression mediated by lenuvirus,proper cell lines and promoters should be selected to obtain high efficiency.

Objective To study the expression changes and profiles of nuclear structure and biogenesis-related genes in rat liver regeneration(LR). Methods Genes involved in nuclear structure and biogenesis were obtained by data collection and literature review.The gene expression changes during LR were checked by Rat Genome 230 2.0 array,and LR-related genes were identified by comparing gene expression difference between partial hepatectomy(PH) and sham operation(SO) groups.Results 406 genes were found to be LR-related.The numbers of initially and totally expressed genes occurring in the forepart(0.5-4 hours after PH) of LR,the prophase(6-12 hours after PH),the metaphase(12-66 hours after PH),and the anaphase(72-168 hours after PH) were 200,29,179,5 and 374,290,1?876,603,respectively.The numbers of their up-regulation and down-regulation were 1?224 and 496 times.The elevated transcription levels occur red to the nuclear organization and biogenesis-involved genes in the prophase and the metaphase of LR and to the nuclear membrane,nuclear matrix and nucleoplasm-involved genes in the metaphase;nuclear chromosome,nucleolus and nuclear functional protein complex-involved genes were up-regulated in the metaphase and the anaphase.Conclusion The expression of nuclear structure and biogenesis-related genes was active during LR,and it was tightly related with LR.