Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

OBJECTIVE: To validate the method for microbial limit test of Niuhuang jiedu tablet.METHODS: The recovery rates of 5 kinds of test organisms including E.coli treated by Niuhuang jiedu tablets were determined by routine method,culture medium dilution method,centrifugation and membrane-filter method,respectively.RESULTS: The centrifugation and membrane-filter method was suitable for determination of the bacterial number of Niuhuang jiedu tablets,while routine method was applicable for determination of bacterial numbers of mold and yeast and test of control bacteria in the microbial limit test.CONCLUSION: The contaminated bacteria in Niuhuang jiedu tablet can be detected accurately by the method established in this study.

Leukocytes from peripheral blood of 70 normal Chinese,40 males and 30 females, were cultured in vitro according to a modification of Moorhead's method.Chromosome studies were carried out.Table I lists the results obtained including age group,metaphase plates counted and analysed and the chromosome number of each cell.92.4 per cent of 8031 counted cells had the diploid number(2n=46).Chromosome measurements were made from camera lucida drawings.Measurements were estimated to the nearest 0.1 mm on a fine metric scale.The relative length,arm ratio and centromere index of each chromosome were calculated from measurements of 18 haploid chromosome sets of 11 metaphase plates in 6 males and 4 females.The averages,range of variation and 99% confidence limits are listed in Table Ⅲ.Systematic studies of individual chromosomes under the light microscope revealed the following morphological characteristics.The short arms of all 10 acrocentric chromosomes from groups 13—15 and 21—22 could be shown to possess satellites,but in general only 2—8 of these chromosomes at a time carried satellites.In many cells the satellite-carrying chromosomes tended to associate with each other through their satellites.The number of chromosomes involved in each association varied between 2 to 5,occasionally 6.It was not rare to find secondary constrictions to occur regularly in certain regions of chromosomes 1,4,9,13,and 16.Attachments of satellites to such regions of chromosomes 1,4,9,and 13 were also observed.Karyotypic analysis of 357 metaphase plates led us to conclude that in most cases the unequivocal identification of chromosome pairs 4—5,6—12+X,13—15,19—20,and 21—22 was im- possible.Accurate characterisation of these chromosome pairs will have to await further technical advances. The length of the Y chromosomes was systematically measured in order to deter- mine its variability and heritability.Among our sample of 40 male subjects 29 had a Y chromosome slightly larger than chromosome 21,8 slightly smaller or larger than chromo- some 18(long Y chromosome),and 3 smaller than chromosome 21(short Y chromo- some).The heritability of the size of the Y chromosome was established(Fig.Ⅳ). “Spontaneous”aberrations were discovered in 34 of 70 cases.Aberration rates varied from 0.88 to 13 per cent.The relation between aberrations and radiation injuries was briefly discussed.

Objective: To analyze the clinical characteristics of non-diabetic fetal macrosomia and their mothers.Methods: A retrospective case control study design was used to analyze 149 cases of non-diabetic fetal macrosomia and their mothers with the normal oral glucose tolerance test(OGTT) by comparison with 166 randomly included newborns of normal birth weight.Results: More cases of non-diabetic fetal macrosomia were found in the male neonates.The body weight,height,FL and BPD averaged significantly higher in the macrosomia group than in the control,and so did the body weight,height,uterus height,abdominal circumference and gestational age of the mothers.The rates of cesarean section and other obstetric complications were also higher in the macrosomia group.Conclusion: Different from the diabetic macrosomic fetus,the non-diabetic macrosomic fetus is a normal one,the incidence of which is associated with mothers'excessiver pregnancy nutrition as well as with genetic factors.

82 spastic cerebral palsy and normal children were selected, including 27 younger and 27 older spastic cerebral palsy( observation group) ,and 14 younger and older normal children( control group) . The tumor necrosis factor α( TNF-α) levels were measured in the serum with ELISA. The TNF-α levels in the serum of the younger and older spastic CP groups were higher than those of the control groups ( P<0.01 ) . In observation groups, the TNF-α levels of younger spastic cerebral palsy were higher than those of the older (P<0.01). On the contrary, the normal children in the control group of different ages had no difference of TNF-α. TNF-αexpression was signifi-cantly higher in the serum of the spastic CP group than that in the control group ( P<0.01 ) . TNF-α expressed continuously in vivo of children with spastic cerebral palsy involve subsequent brain injury.

Objective To explore the the effect of psychological intervention on emotion and postoperative bladder spasm in elderly patients with benign prostatic hyperplasia. Methods 40 elderly cases with benign prostatic hyperplasia were classified as the control group and were given the routine intervention, the other 40 cases were received psychological intervention at the basis of routine intervention and classified as the observation group. The two groups were treated in Anji county traditional Chinese medicine hospital from September 2015 to January 2017. Results The anxiety and depression in the 2 groups had no significant difference before intervention, and the prognosis in the observation group was better than that in the observation group after intervention. The bladder spasm in the two groups was compared. The third days after the operation, the incidence of bladder spasm in the observation group was 10.0%, which was significantly lower than 45.0% in the control group, and the difference between the 2 groups was statistically significant (P<0.05). Conclusion Psychological intervention has better effect on mood and bladder spasm in elderly patients with prostate hyperplasia. It can help patients to improve their emotional state, reduce anxiety and depression, and ultimately reduce the incidence of bladder spasm, which is worthy of clinical reference.

Objective To study the clinical effect of small incision cataract extraction in the treatment of acute angle-closure glaucoma with cataract.Methods Fifty-eight patients with acute angle-closure glaucoma combined with cataract were selected,and according to the digital table they were randomly divided into two groups:control group and observation group.The patients in the control group were treated by conventional method.The patients in the observation group were treated with small incision cataract.The changes of intraocular pressure,anterior chamber depth and visual acuity before and after treatment were observed.The incidence of complications and the satisfaction of treatment were compared between the two groups.Results There were no significant differences in IOP,visual acuity and anterior chamber depth between the two groups before treatment(all P>0.05).After treatment,the intraocular pressure,anterior chamber depth and visual acuity of the observation group were (10.2±4.0)mmHg,(3.3±0.5)mm,(0.85±0.08),respectively,which of the control group were (17.5±5.0)mmHg,(3.2±0.5)mm and (0.50±0.06),respectively.The improvement of intraocular pressure and anterior chamber depth of the observation group was more significant than the control group(t=0.120,0.520,1.012,all P<0.05).The satisfaction rate of the observation group(96.6%) was higher than that of the control group(75.8%)(χ2=9.823,P<0.05).There was no significant difference in the incidence of complications between the two groups(P>0.05).Conclusion Small incision cataract extraction is an effective treatment for acute angle-closure glaucoma complicated with cataract,and the postoperative complications are less.The patients are satisfied with the treatment and deserve to be popularized.

Objective To study the curative effect of traditional chinese medicine combined with noninvasive ventilation on respiratory failure in patients with acute exacerbation of chronic obstructive pulmonary disease.MethodsThe patients were divided into experimental group (n=37) and control group (n=37).The experimental group was treated with traditional chinese medicine combined with noninvasive ventilation.The control group was treated with noninvasive ventilation method.The heart rate, blood gas analysis index, ventilation index and complication were compared between the two groups before and after treatment.ResultsAfter treatment, the levels of PaO2 in the two groups were not significantly different.The heart rate and PaCO2 of the experimental group were significantly lower than those of the control group, while the pH value and SaO2 were significantly higher than those in the control group (P< 0.05), and the total incidence of complications was 21.62%, which was significantly lower than that of the control group (64.86%, P<0.05).The time of infection control window, the highest PEEP and the time of mechanical ventilation were significantly shorter than those of the control group (P< 0.05).ConclusionThe treatment of patients with acute exacerbation of chronic obstructive pulmonary disease(COPD) combined with noninvasive ventilation therapy can significantly improve the heart rate and blood gas analysis indexes, and optimize the noninvasive ventilation efficiency, control lung infection as soon as possible, shorten the time of noninvasive ventilation and reduce the side effects that noninvasive ventilation brought about.

15℃ . Conclusion The changes of density of different strains of Escherichia coli in the undernourished water samples closely associated with the strain ' characteristics, environment temperatures and preserving time, showed significantly differences.

AIM: To evaluate the value of urethra perfusion using 0.5% povidone iodine liquid (PVP-I) in treatment of patients with chronic urethritis and prostatitis. METHODS: Outpatients with chronic urethritis and prostatitis were assigned to two groups randomly. 38 cases in treatment group were received urethra perfusion with 0.5 % PVP-I liquid plus minomycin, po, for two weeks. 42 cases in control group were received minomycin only. RESULTS: The rates of cure and total efficacy were better in treatment group than in control group (P