Objective To study the expression of malignant melanoma(MM) related genes by cDNA microarray technique. Methods mRNA, extracted from tissues of patients and normal controls, was reversely transcripted into cDNA and marked with 33P. The cDNA probes were hybridized to cDNA microarrays, which contained 2000 human genes each array. The down-regulation of two co-differentiated expressed genes was confirmed by quantitative real-time RT-PCR. Results Different expression between MM and normal controls was found in 4.7%-6.15% of genes by more than 2 times,0.75%-1.4% by more than 5 times, and 0.45-0.5% by more than 10 times. These genes were pro-oncogenes, tumor suppressor genes, genes related to apoptosis, cell cycle related genes, and so on. Three genes were down-regulated in all of the patients. Two of those genes, histidine triad nucleotide-binding protein (HINT) and RBP1-like protein (BCAA), were down-regulated, as identification by quantitative real-time RT-PCR. Conclusions cDNA microarray can be used effectively to reveal melanoma gene expression profiling for the propose of carcinogenesis study. HINT and BCAA are the first reported genes down-regulated in MM. However, further studies are needed for their expressive specificity and mechanism in MM.

AIM: To explore the relationship between hypermethylation of p15INK4B gene and the pathogenesis of hematopoietic malignances. METHODS: The expression and methylation of p15INK4B gene and the expression of DNA methyltransferase genes (DNMTs) in bone marrow cells from 54 cases with hematopoietic malignances were detected by RT-PCR, Western blot, and methylation-specific PCR. RESULTS: The p15INK4B gene was methylated more often in high-risk myelodysplastic syndrome (MDS) patients, patients at blast phase of chronic myeloid leukemia (CML-BP) and acute leukemia patients than that in low-risk MDS patients (P

Hemodynamics ; Humans ; Hypertension, Portal ; etiology ; physiopathology ; surgery ; Liver Cirrhosis ; complications ; physiopathology ; surgery ; Liver Transplantation ; Postoperative Period

Conjunctival Impression Cytology (CIC) method was modified to assess vitamin A level more exactly and simply in China. The infants (

Objective:To compare the efficacy and safety of broad-band intense pulsed light (OPT-IPL) versus narrow-band intense pulsed light (DPL) in the treatment of rosacea-associated erythema and telangiectasia.Methods:Fifty-four rosacea patients who received treatment with intense pulsed light were collected from Laser Department, Hospital of Dermatology, Chinese Academy of Medical Sciences from October 2016 to December 2019, and clinical data were retrospectively analyzed. Their age ranged from 19 to 56 years, and disease duration ranged from 0.2 to 10 years. Of the 54 patients, 22 were treated with OPT-IPL, and 32 were treated with DPL. All patients completed at least one session of treatment and follow-up. Therapeutic efficacy was evaluated by using clinician erythema assessment (CEA) and physician global assessment (PGA) scales, and adverse reactions were assessed. A generalized linear mixed model was used to analyze differences in CEA and PGA scores among different groups and treatment sessions.Results:In the OPT-IPL group, 22, 17 and 10 cases completed 1, 2 and 3 sessions of treatment respectively, with the energy fluence being 16.57±1.21 J/cm 2. In the DPL group, 32, 25 and 16 cases completed 1, 2 and 3 sessions of the treatment respectively, with the energy fluence of 9.76±0.61 J/cm 2. Before the start of treatment and after 1, 2 and 3 sessions of treatment, the CEA scores were 2.38±0.84, 2.29±0.75, 1.94±0.66 and 1.90±0.66 respectively in the OPT-IPL group, and 2.25±0.77, 2.16±0.77, 1.84±0.81 and 1.47±0.81 respectively in the DPL group. As far as the CEA score was concerned, there was no interaction between the groups and treatment sessions ( F=0.57, P=0.638) , and no significant difference between the OPT-IPL group and DPL group ( F=0.84, P=0.360) , but a significant difference was observed among different sessions of treatment ( F=17.90, P< 0.001) , and the CEA score gradually decreased along with the increase of treatment sessions compared with that before treatment (all P< 0.05) . After 1, 2 and 3 sessions of treatment, the PGA scores were 0.39±0.71, 0.82±0.92 and 0.55±0.80 respectively in the OPT-IPL group, and 0.61±0.77, 1.34±1.09 and 1.53±1.38 respectively in the DPL group. As far as the PGA score was concerned, there was no interaction between the groups and treatment sessions ( F=1.62, P=0.202) , and no significant difference between the OPT-IPL group and DPL group ( F=3.93, P=0.050) , but there was a significant difference among different sessions of treatment ( F=19.33, P< 0.001) . Compared with the PGA score after 1 session of treatment, the PGA score gradually increased along with the increase of treatment sessions (all P< 0.001) . After treatment, no adverse reactions, such as blisters and crusts, occurred in either of the 2 groups, and there was no significant difference in the incidence of pigmentation, erythema aggravation, papules or increase in papule count between the 2 groups (all P> 0.05) . Conclusion:The efficacy and safety of DPL are comparable to those of OPT-IPL in the treatment of rosacea-related erythema and telangiectasia, but lower energy fluence is required.

Objective To investigate the relationship between the change of HDL level and the severity in patients with sepsis.Methods The levels of HDL were measured in 68 patients with sepsis who were divided into light,middle and heavy group by APACHEⅡscore after final diagnosis of 0,1,2,3,7,14.28 days.Results The levels of HDL in patients with sepsis were lower than those of the normal:the levels of HDL began to decrease at 0 day,got to lowest point after 3 days,and up-regulated gradually after 7 to 28 days.The levels of recovery were the highest in light group,and the lowest in heavy group.Conclusion The levels of HDL in patients with sepsis are low,which is related with the severity of sepsis.

Haplotypes ; Humans ; Neoplasms ; genetics ; Polymorphism, Single Nucleotide ; genetics

Objective: To investigate the effect and mechanism of emodin (EMO) on human pancreatic cancer cell line BxPC-3 in vivo. Methods: After the pancreatic cancer model in nude mice was established, the mice were divided into four groups: control group (NS 0.2mL/d by i.p. injection), EMO group (EMO 40mg?kg-1?d-1 by i.p. injection), and Gemcitabine (GEM) group (GEM 80mg/kg, twice/week by i.p. injection) with 8 mice each group. After 2 weeks of administration, the mice were sacrificed, detected the body-weight change of nude nice before and after the experiment, and recorded the growth inhibition rate of tumor (TGI). Immunohistochemical (IHC) staining for ki-67 and terminal deoxynucleotidyl transferase-mediated nicked labeling assay (TUNEL) were undertaken to detect the cell proliferation and cell apoptosis in tumor tissue in xenograft nude mice. Results: The inter-group comparisons in body-weight of nude mice showed no significant difference in comparing group NS(27.0?1.64)g with group EMO(25.1?1.58)g and GEM(25.6? 1.47)g.The EMO group was 38.46%, the GEM group was 44.23%. The inter-group comparisons in immunohistochemical analysis of ki-67 showed significant difference in comparing group NS IOD(219.5?17.98) with group EMO IOD(146.6? 11.57)and GEM IOD(139.5?12.55), (P

Objective To evaluate the effect of Wnt5A gene overexpression on cytoskeletal proteins of melanocytes after the plasmid containing the Wnt5A gene is transfected into primary melanocytes.Methods In vitro cultured primary human melanocytes were divided into three groups:blank control group receiving no treatment,negative control group transfected with endotoxin-free pcDNA3.1 (+)empty vector by Lipo3000 in Opti-MEM medium,Wnt5A plasnid group transfected with endotoxin-free pcDNA3.1 (+) vector containing the Wnt5A gene by Lipo3000 in Opti-MEM medium.After the transfection,quantitative PCR (qPCR) was performed to measure the mRNA expression of Wnt5A,ras-related C3 botulinum toxin substrate 1 (Rac1),filamentous actin (F-actin) and β-tubulin,Western blot analysis to determine the protein expression of Wnt5A,receptor tyrosine kinase like orphan receptor 2 (ROR2),Rac1,F-actin and β-tubulin,and an immunofluorescence assay (IFA) to observe the expression of cytoskeletal proteins.Results qPCR showed significant differences in the mRNA expression of the Wnt5A gene and its downstream genes Rac1 and F-actin among the Wnt5A plasmid group,negative control group and blank control group (F =1374.179,112.576,66.458,respectively,all P ＜ 0.01),but there was no significant difference in the mRNA expression of β-tubulin among the three groups (P ＞ 0.05).Additionally,the Wnt5A plasmid group showed significantly higher mRNA expression of Wnt5A,Rac1 and F-actin compared with the blank control group and negative control group (all P ＜ 0.05).As Western blot analysis revealed,compared with the blank control group and negative control group,the Wnt5A plasmid group showed significantly higher Wnt5A protein expression (both P ＜ 0.05),but significantly lower protein expression of Rac 1,ROR2 and F-actin (all P ＜ 0.05).However,no significant difference in β-tubulin protein expression was observed among the three groups (P ＞ 0.05).IFA showed no obvious difference in the fluorescence intensity of β-tubulin or F-actin between the Wnt5A group and the two control groups,but melanocytes showed larger size and increased number of dendrites,and the cytoskeleton changed dramatically with varying fluorescence intensity of F-actin,fuzzy texture,fractured or locally clustered tonofilaments in the Wnt5A group.Conclusion The overexpression of the Wnt5A gene in melanocytes can regulate the mRNA and protein expression of cytoskeletal proteins,nake melanocytes larger and more dendritic,and cause changes in the cytoskeleton,which may facilitate the transportation of melanosomes,and participate in the occurrence of hyperpigmented diseases.