Objective:To explore influence of large dose atorvastatin therapy on ventricular remodeling in rats after myocardial infarction.Methods:A total of 48 male SD rats were randomly and equally divided into myocardial in-farction (MI)control group (received deionized water gavage),routine dose group (received atorvastatin 20mg/kg gavage four weeks before and after MI model was established)and large dose group (received atorvastatin 80mg/kg, in the same way as routine dose group).On two days after MI,hematoxylin eosin (HE)staining was used to detect pathological changes of myocardial tissue;four weeks later,Masson staining was used to evaluate myocardial fibrosis while echocardiography was used to assess cardiac chamber size and heart function,the results were compared among three groups.Results:Pathologic change:On two days after MI,color of stained myocardial tissue was uneven, muscular fiber arranged in turbulence,there were partial dissolved muscle focus and blockbuster collagenous fiber in MI control group;color of stained myocardial tissue was lack of even,arrangement of muscular fiber was irregular, there were less dissolved muscle focus and collagenous fiber in routine dose group;color of stained myocardial tissue was slightly uneven,muscular fiber still arranged in rules and there was very few dissolved muscle focus and still more survived myocardium in infarct area,and small amount of collagen fibrosis was found in myocardial cell clear-ance after four weeks in large dose group.Change of left ventricular structure and function:Compared with MI con-trol group and routine dose group,the left ventricular end-diastolic dimension [LVEDd,(7.68±0.54)ml vs.(7.06 ±0.68)ml vs.(6.37 ± 0.40)ml] significantly reduced in large dose group, left ventricular ejection fraction [LVEF,(33.0±7.9)% vs.(39.3±2.8)% vs. (48.7%±9.1)%]significantly rose (P <0.05,or <0.01);but those indexes no significant difference between MI control group and routine dose group (P =0.059,0.146 respec-tively).Conclusion:Large dose atorvastatin may significantly relieve ventricular remodeling after infarction.

Objective: To study the chemical constituents of the roots of Ilex cornuta. Methods: The compounds were isolated and purified by silica gel, Sephadex LH-20, medium pressure column chromatography, and semi-preparative liquid chromatography, and their structures were elucidated by chemical properties and spectroscop analyses. Results: Eight compounds were isolated and their structures were identified to be β-sitosterol (1), lupeol (2), betulonic acid (3), hede-ragenin (4), 3β-acetoxy-28-hydroxyurs-12-ene (5), ursolic acid (6), 19α-hydroxy ursolic acid (7), 3β-acetoxy-ursolic acid (8), 23-hydroxyl-methyl ursolate (9), heptanoic acid (10), β-daucosterol (11). Conclusion: Compounds 4,5,8, and 9 are obtained from this genus for the first time, and compound 3 is obtained from this plant for the first time.

Objective To investigate changes of coagulation function before and after percutaneous coronary intervention(PCI) in patients with coronary heart disease ,so as to provide evidence and guidance for clinical treatment .Methods A total of 78 patients with coronary heart disease who received PCI from September 2013 to August 2014 were regarded as subjects .The thrombin time (TT) ,thrombin original time (PT) ,activated partial thromboplastin time (APTT) and levels of fibrinogen (Fg) and antithrombin (AT) were detected before surgery and 4 ,14 h after surgery ,and conducted analysis and comparison .Results In the 78 cases of pa‐tients with coronary heart disease ,56 cases with single vessel disease ,19 cases with double vessel disease ,3 cases with three le‐sions ,and the total lesion count was 115 ;diseased vessels were implanted 101 ,35 and 8 stents respectively ,and a total of 144 stents were implanted .The TT and APTT before PCI was (14 .1 ± 0 .4) and (38 .4 ± 1 .5) s respectively ,and was (64 .1 ± 5 .8) and (73 .5 ± 15 .7) s respectively 4 h after surgery ,the differences were statistically significant (P<0 .05);while there were no statis‐tically significant differences of TT ,APTT ,PT ,and levels of Fg 24 h after surgery compared with those before PCI(P>0 .05) ,and these indicators were back to normal .Conclusion Routine monitoring TT and APTT 4 ,24 h after PCI could reflect coagulation function ,while routine monitoring may not be necessary for patients whose levels of AT are back to normal 4 h after surgery .

Objective To investigate the association between serum uric acid (SUA)levels and outcome in acute ischemic stroke.Methods A total of 388 acute ischemic stroke patients were included in the prospective case study.Data on demographic characteristics, risk factors for life style, history of disease, the admission SUA and outcome at discharge were collected for all participants.Results Mean serum uric acid levels were (284.5±83.6) μmol/L in the normal uric acid? group, and(455.3±78.1)μmol/L in the high uric acid group(P＜0.01).There were significant differences in National Institutes of Health Stroke Scale (NIHSS) score at two weeks between the high UA group and the normal UA group [(11.0±6.0) vs.(11.0±5.3),P=0.048], and also in early neurological improvement (P=0.048).A significant difference was found in serum uric acid level between patients with good versus poor neurological function outcome (P =0.045).Conclusions There is the association between admission serum uric acid levels and outcome in acute ischemic stroke.Elevated serum uric acid level predicts a lower chance of a poor outcome in acute ischemic stroke.

Satellite RNA ofBaMV (satBaMV), a single-stranded positive-sense RNA of 836 nucleotide [excluding 3′ poly(A) tail]containing a single open reading frame which encodes a nonstructural protein of 20 ku (P20), depends on BaMV for its replication and encapsidation. P20 is a nucleic-acid-binding protein, which facilitates the long distance movement of satBaMV in plants.Protein-protein interactions were analyzed by a bacterial two-hybrid system and pull-down assays to investigate whether P20 could self-associate and/or interact with the helper virus proteins. Self-interaction of P20 was the strongest among the viral protein-protein interactions detected in vivo and in vitro. Significant interactions of P20 with methyltransferase (MET) and capsid protein (CP) of BaMV were evidenced. Interactions among triple gene block protein 1, 2 and 3 (TGBp1, TGBp2 and TGBp3) of BaMV were also significant. BaMV CP also exhibited a strong self-interaction and strong affinity to TGBp1, TGBp2 and TGBp3. By deletion analysis,the minimal region delineated for the self-interaction of P20 was the N-terminal 15 amino acids which include the RNA binding motif of P20. N-terminal deletion resulted in the loss of self-interaction of P20. Deletion analysis also confirmed the importance of β-sheet structure of P20 in the P20-P20 interaction. P20 showed significant interactions with two host (Nicotiana benthamiana) proteins,cytochrome-C reductase and β-tubulins. The homotypic and heterotypic interactions of BaMV proteins and P20 in vivo thus suggest that the protein-protein interactions may directly exert an effect on the BaMV and satBaMV RNA movement as a protein complex in the infected host plants.

Bacterial Infections ; epidemiology ; etiology ; Cerebral Hemorrhage ; diagnosis ; etiology ; therapy ; Craniocerebral Trauma ; diagnosis ; etiology ; therapy ; Female ; Fetal Membranes, Premature Rupture ; Gestational Age ; Humans ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; diagnosis ; etiology ; therapy ; Pregnancy ; Risk Factors ; Tomography, X-Ray Computed

Objective To translate the English version of Diabetes Continuity of Care Scale (DCCS) into Chinese and to test the reliability and validity of the Chinese version of DCCS in diabetes mellitus patients.Methods Totally 245 patients with diabetes mellitus were recruited and were investigated by the Chinese version of DCCS.Results The internal consistency coefficient of the Chinese version of DCCS ranged from 0.728 to 0.942,which was ＞0.70;split half coefficient r=0.784,and the test-retest was 0.864 while which of each dimension ranged from 0.785 to 0.886.The content validity index was 0.934.Factor analysis got five factors,which explained 76.574％ of the total variance;the factor loading of each item was ＞0.4.Conclusions The Chinese version of DCCS has been proved to be reliable and valid.It can be used as a valid tool for the measurement of continuity of care in patients with diabetes mellitus.

Objective To investigate the clinical application value of real‐time PCR in the detection of bloodstream infection pathogens .Methods A total of 92 blood samples from 80 patients in our hospital were collected for conducting real time PCR de‐tection and conventional blood culture .The sensitivity and specificity were compared between the two methods .Results Among 92 samples ,66 samples (71 .7% ) were negative in both assays .Ten different pathogens were detected by either blood culture system or real‐time PCR or by both methods .Seven positive samples were detected by both assays .The consistence of the two methods was 79 .3% .The negative predictive value of real‐time PCR was 0 .94 ,the sensitivity was 0 .64 and the specificity was 0 .82 .Among them ,15 samples were positive in real‐time PCR ,while negative in blood culture system ,4 samples were positive in the blood cul‐ture ,whereas were negative in the real‐time PCR .The pathogens cultured in 2 samples were not in the detection range of real time PCR ,moreover real time pCR could not detect Candida glabrata .Conclusion Real time PCR is a valuable method for rapidly detec‐tion the sample of bloodstream infection ,but cannot completely replace the blood culture test .

Objective To observe the clinical effects and intubation condition of different doses of domestic cisatricurium(CA) in children. Methods Forty-five peadiatric patients aged 2 to 12 years old scheduled for selective surgery were randomized to three groups of 2 ED_(95) CA(0. 08 mg/kg), 3ED_(95) CA(0. 12 mg/kg) and 4ED_(95) CA(0. 16 mg/kg) (C_2, C_3, C_4 groups). Neuromuscular blockade was monitored with TOF-Guard. The conditions for trachea[intuDation were scored and the onset time, clinical duration, recovery index were recored. ResultsTracheal intubation was performed successfully in all patients. The conditions for tracheal intubation were scored greater 5 points in groups of C_3 and C_4 ,which was only 33. 3% of patients in group C_2. The onset time was shortened and the duration of action was prolonged as the dose of CA increased. But the recovery index of three groups were smilar. Conclusion An increase of CA dosage can shorten the onset time and prolong the duration of action and 3ED_(95) CA is suitable for tracheal intubation in children.

Objective To investigate the effects and the mechanism of telmisartan and pyridoxamine on oxidative stress in spontaneously hypertensive rats(SHR).Methods SHRs(male,20 weeks of age) were randomly divided into four groups (n＝ 12 for each):hypertension control (HC) group (2 ml of distilled water),telmisartan group[T,6 mg/(kg · d)],pyridoxamine group[P,200 mg/(kg · d)]and combined group(TP,6 mg/kg telmisartan and 200 mg/kg pyridoxamine per day).Treatments were continued for 16 weeks.The normal control group included 13 WKY rats and received gastric lavage with distilled water.SBP of tail artery was measured during the intervention ervey 2 weeks.The levels of AGEs,SOD and MDA were measured by ELISA,xanthine oxidase and thiobarbituric acid methods after the intervention.Expressiones of NF-κBp65,ERK1 and ERK2 in renal tissue were detected by immunohistochemistry.Expression of RAGE in the renal cortex was investigated by Western blot.Results SOD activity was decreased in the HC group.The levels of AGEs,MDA,RAGE and the activations of NF-κBp65 and ERK1/2 were increased in the HC group (t＝4.53,5.52,2.93,al1 P＜0.05).After the 16 weeks' intervention,SOD activity was elevated in T,P and TP groups compared to that in HC group (P＜0.05).The positive expressiones of NF-κBp65,ERK1 and ERK2 were significantly reduced in T,P and TP groups compared to those in HC group (F＝20.13、148.82、18.70,all P＜0.05).All the positive expressiones of NF-κBp65,ERK 1and ERK2 were lowest in the TP group versus T and P groups (t ＝ 3.58、2.84,P ＜ 0.05).Conclusions Telmisartan and pyridoxamine can alleviate the oxidative stress in spontaneously hypertensive rats,which may result from the blocking effect of Ang Ⅱ,the reduction of AGEs-RAGE and inhibiting the signal pathways of ROS,NF-κBp65 and ROS-ERK1/2.