Objective Through To analyzing analyze the pathogen distribution and resistance analysis of elderly patients with pulmonary infection,in order to provide basis for clinical rational administration.Methods The clinical data of 285 elderly patients diagnosed as pulmonary infection were selected,and their sputum specimens cultivation were collected for statistical analysis,and the pathogenic bacteria distribution and drug resistance were analyzed.Results 292 strains of pathogenic bacteria were detected from 285 patients,of which 204 strains of gramnegative bacteria,accounting for 69.8％,mainly including pseudomonas aeruginosa,acinetobacter baumannii and klebsiella pneumoniae,while 84 strains of gram-positive bacteria,accounting for 28.8％,mainly including staphylococcus aureus and 4 strains of fungus,accounting for 1.4％.Different pathogens showed different degrees of antimicrobial resistance:pseudomonas aeruginosa,acinetobacter baumannii,klebsiella pneumoniae and e.coli had highly sensitivity for imipenem and meropenem,which were secondly sensitive to amikacin and highly resistant to ampicillin;Staphylococcus aureus and epidermis staphylococcus were all sensitive to linezolid and vancomycin,which were highly resistant to benzyl penicillin.Conclusion Gram-negative bacilli is the main bacteria for patients with pulmonary infection,which has higher antimicrobial drug resistant rate,and antimicrobial agents should be rationally used according to drug susceptibility results.

Objective:To investigate the effects of microgroove surface morphology on the adhesion and proliferation of the human gin-gival fibroblasts(HGFs).Methods:Microgroove surfaces of titanium were fabricated by photolithography with parallel grooves of 15,30 or 60 μm in width and 5 μm or 10 μm in depth.The groups of the samples were denoted as T15 /5,T15 /10,T30 /5,T30 /10,T60 /5 and T60 /10.Smooth titanium surface(T0)was used as the control.The surface topography was observed by enviroment SEM(ES-EM).HGFs were cultured on the topographically modified surfaces.Morphology was observed by SEM.Cell proliferation was examined by CCK-8 kit.Results:HGFs on the microgroove surfaces had “contact guidance”parallel to the microgrooves,whereas the cells on T0 were oriented randomly.Cell proliferation was promoted and kept for longer period on T60 /10 surface.Conclusion:Surfaces of mi-crogrooves with increasing groove width and depth may achieve “contact guidance”for HGFs and promote cell proliferation.

Objective To discuss effect of serum immunoglobulin M (IgM) and superoxide dismutase (SOD) by percutaneous epidural steroid injections in patients with lumbar disc herniation.Methods 137 patients with lumbar disc herniation were selected in Central of Hospital of Wuhan,all patients were randomly assigned joint group and O3 group, all patients were given ozone (O3) injection treatment, before treatment the joint group was given percutaneous epidural steroid injections, the joint group on the basis of O3 injection method was divided into multi-way group and routine group, followed up for 6 months, analyzed all patients therapeutic effect and recurrence rate,used enzyme-linked immunosorbent method to detect patients with serum IgM, SOD level, Pearson analysis method was used to analyze the relationship between the two groups.Results The joint group treatment effective rate was 91.89%, O3 group treatment effective rate was 79.73%, the former was higher than the latter, the difference was statistically significant ( P<0.05), The joint group serum IgM, SOD level was better than O3 group(P<0.05), In six months, multi-way group recurrence rate was 10.81%, routine group recurrence rate was 18.92%,O3 group recurrence rate was 31.08%, multi-way grou P

Objective　To evaluate the value of gradient-echo chemical shift imaging in detection of lipid-containing abdominal lesions. Methods　35 patients with abdominal lesions were divided into 2 groups according to whether or not they contained lipid. Breath-hold in-phase(IP) and opposed-phase(OP) acquisitions were performed. The demonstration of these lesions on IP and OP was compared. The signal intensity of these lesions on both images was measured. The percentage of signal intensity variation on IP and OP was calculated and statistically analyzed with non-parametric method between the 2 groups. Results　For lesions containing lipid, the signal intensity dropped significantly from IP to OP, with median value of signal intensity variation attaining 37.1%. On the other hand, for lesions not containing lipid, the signal intensity did not change obviously between IP and OP, whose median value of signal intensity variation was 3.3%. The difference between the 2 groups was statistically significant (u=4.56，P<0.01). Conclusion　Gradient-echo chemical shift imaging is an easy and practical way, helpful to detection of lipid component within abdominal lesions.

The effects of hepatocyte growth factor(HGF) on the secretion of vascular endothelial growth factor(VEGF) in cultured bovine coronary artery endothelial cells(BCAEC), bovine coronary artery smooth muscle cells(BCASMC) and mouse cardiomyocytes were observed in vitro . The concentrations of VEGF in BCAEC control and HGF group at 12h were 10.51?2.90pg/ml and 9.31?2.78pg/ml, respectively. The concentrations of VEGF at 12h in BCASMC control and HGF group were 3.35 and 3.93 times of that at 3h respectively, the concentration of VEGF at 3h and 12h in BCASMC HGF group was 2.06 and 2.42 times respectively, of that in control group. The concentrations of VEGF at 12h in cardiomyocyte control and HGF group were 5.43 and 4.09 times, respectively, of that at 3h, and the concentrations of VEGF at 3h and 12h in cardiomyocyte HGF group was 2.74 and 2.06 times, respectively, of that in control group. These results indicated that BCAEC, BCASMC and mouse cardiomyocytes could secrete VEGF, and HGF could promote VEGF secretion in BCASMC and cardiomyocytes, but not in BCAEC.

The effects of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) on proliferation of bovine coronary artery endothelial cells (BCAEC) and smooth muscle cells (BCASMC) were studied in vitro. BCAEC and BCASMC were isolated and cultured and divided into control group, VEGF (50ng/ml) group and HGF (50ng/ml) group. Cells proliferation was measured using MTT method. The results showed that the OD values of control, VEGF, and HGF group in BCAEC cultures were 0.23?0.02, 0.58?0.10, and 0.42?0.12, respectively, and those in BCASMC were 0.31?0.08, 0.45?0.09, and 0.40?0.11, respectively. The proliferation ratios of BCAEC and BCASMC induced by HGF were 152.2%?33.8% and 45.2%?25.3%, respectively, and that by VEGF were 82.6%?18.7% and 29.0%?20.4%, respectively. The results suggested that HGF could promote proliferation and migration of BCAEC and BCASMC, while VEGF could promote proliferation of BCAEC but not BCASMC. The effect of HGF on BCAEC was stronger than that on BCASMC, and the induction strength of HGF was higher than VEGF.

Objective To evaluate the usefulness of three dimensional dynamic contrast enhanced magnetic resonance angiography(3D DCE MRA) in the diagnosis of vena caval abnormalities. Methods 3D DCE MRA was performed on fifty two patients with suspected vena caval anomaly, thrombosis, stenosis, occlusion, or compression. Image quality and the presence of venous abnormalities and collaterals were evaluated. Comparison with conventional venacavography( n =15) and surgical findings( n =18) was made. Results 3D DCE MRA images were of diagnostic quality without artifact for all 52 patients. 3D DCE MRA clearly showed vascular anatomy, anomaly and various disorders. The site, extent, cause of venous obstruction, and collateral pathways were well depicted. According to venography or surgery, the sensitivity and specificity of 3D DCE MRA in the diagnosis of abnormalities of vena cava were both 100%. In addition, it had advantages regarding demonstration of extravascular lesions and multiplanar capabilities. Conclusion 3D DCE MRA is simple to perform and accurate in detecting various vena caval abnormalities.

Objective To explore the diagnosis, surgical treatment and prognosis of thymoma complicated with myasthenia gravis(MG). Methods A retrospective analysis was performed in 33 thymoma patients with MG, who underwent surgical treatment. Results The total effective rate of operation was 75.8%, and the 5 and 10 years survival rates of the patients after operation were 85% and 78.7%, respectively. According to the Masaoka staging system, the 5 and 10 years survival rates of stages I and II were obviously higher than those of stages III and IV (P

Over the past ten years studies in animals and human have supported the links between inflammation and tumors. The inflammatory cells and agents found in tumors play an important role in the pathogenesis of malignant disease facilitating tumor growth, invasion and metastasis. This article reviewed the molecular mechanism of inflammatory action in promoting tumors and suggested that inflammatory cytokine network is more likely to contribute to immunosuppression than to mount an effective host anti-tumor response. [

Objective:To investigate the effect of microgroove surfaces of titanium on the adhesion and cell cycle progression of human gingival fibroblasts(HGFs).Methods:Microgroove titanium surfaces were fabricated on silicon plate by photolithography with parallel grooves:1 5,30 and 60 μm in width and 5 μm and 1 0 μm in depth,the groups were denoted as T1 5 /5,T1 5 /1 0,T30 /5,T30 /1 0, T60 /5 and T60 /1 0,respectively.Smooth titanium surfaces (T0)were used as the controls.Surface topography were observed by ES-EM.HGFs were cultured on the microgroove surfaces.Morphology of “contact guidance”was observed by immunofluorescence tech-nique.Cell cycle distribution was analyzed by Flow cytometry.Results:HGFs on the microgroove surfaces had “contact guidance”par-allel to the microgrooves,whereas the cells on T0 were oriented randomly.T60 /1 0 group had the highest percentage of S phase cells, followed by T30 group and T1 5 group,but still higher than that in the control group.In groups with higher groove width (T60 group and T30 group),the increase of groove depth benefited the increase of S phase percentage,while in T1 5 group,the increase of groove width decreased the S phase percentage.Conclusion:Surfaces of microgrooves with different dimensions achieved “contact guidance”for the cultured HGFs.The surfaces with increasing groove width and depth benefit the cell cycle progression.