Objective To make clear the relationship between adenoma,malignant transformation of ade- noma and carcinoma in gallbladder.Methodes The nuclear morphometry was performed with a TJTY-300 Auto- matic Image Analyser and PCNA was measured with immunohistochemisty in 25 cases of carcinoma,20 cases of adenoma and 3 cases of normal gallbladder.Results (1) Cell DNA content,atypia and PCNA index were in- creased gradually from the normal cell to dysplasia to malignant transformation of adenoma to carcinoma in gallblad- der;(2) Aneuploidy in gallbladder adenoma and PCNA index had significant relationship to the size of adenoma and gallstones (P

Objective To find out the distribution and changing trend of pathogens in positive blood cultures. Methods To analyze 183 isolates from 2010 blood specimens then the results were compared with the relative clinical data. Results A total of 183 strains were isolated from all blood specimens, of which 139 strains (76.0%) were identified as pathogens, where 69 strains (49.6%)being Gram-positive cocci, 57 strains (41.0%) gram-negative bacilli, and 13 strains (9.4%) fungi, respectively. The other 44 strains (24.0%) were contaminated. The major pathogens in 139 strains were Coagulase-negative staphylococci (40 strains, 28.8%), Pseudomonas aeruginosa (17 strains, 12.2%), Escherichia coli (15 strains, 10.8%), fungi 13 strains (9.4%) and Staphylococcus aureus (9 strains, 6.5%). Conclusion The dominant pathogens in positive blood cultures were gram-positive cocci, while gram-negative bacilli cultures were becoming less.

Objective To estimative the relationship between ras gene mutation,PCNA overexpression and the recurrence and prognosis of gallbladder cancer.Methods PCNA was studied by ABC immunohistology, ras gene mutation by PCR-RELP analysis.Results Histology,Nevin staging and operative methods were relevant to the prognosis of gallbladder cancer.The patient with stag IV,V gallbladder cancer had a poor prognosis;radical operations could prolong patients survival time.The mortal risk of patients with ras gene mutation was 1.62 times higher than that without ras gene mutation,with high overexpression of PCNA was 2.2 times higher than that with low expression of PCNA. Conclusions Differentiation degree, Nevin′s staging, mutation of ras gene and overexpresion of PCNA could be the prognostic factors in gallbladder cancer,and could help to select the operative procedure and to eluvate the surrival.

Objective:To evaluate the cytotoxicity of sodium hypochlorite(NaOCl) and chloramine-T at a same available chlorine concentration. Methods: L929 fibroblasts were used to test the cytotoxicity of NaOCl and chloramine-T at a same available chlorine concentration (0.500 0%, 0.250 0%,0.200 0%, 0.150 0%, 0.125 0%, 0.062 5%). Cell viability after 2 h, 30 min or 10 min exposure were measured by MTT assay. Results: Except the 0.500 0%-2 h group and 0.062 5%-30 min group, cell viability of NaOCl groups were higher than chloramine-T group(P<0.05). Conclusion: NaOCl solution has less cytotoxicity than chloramine-T at a same available chlorine concentration lower than 0.250 0%.

Objective To investigate the effect of early rehabilitation treatment in the patients with small cerebral hemorrhage and its possible mechanisms .Methods 133 patients with cerebral hemorrhage were randomly divided into the routine treatment , non-early rehabilitation and early rehabilitation groups and given the routine drug treatment .The early rehabilitation group and the non-early rehabilitation group were additionally given the rehabilitative training after 2 d and 14 d respectively .The scores of Fugl-Meyer reassessment and the Barthel index were assessed before training and after 4-week training ,and the serum levels of circulat-ing endotheial progenitor cells(EPCs) and vascular endotheial cell growth factors(VEGF) were detected .Results Compared with the routine treatment group and the non-early rehabilitation group ,the scores of Fugl-Meyer reassessment and the Barthel index af-ter treatment in the early rehabilitation group were significantly increased (P< 0 .01) .The serum levels of circulating EPCs and VEGF in the early rehabilitation group were also significantly increased compared with the routine treatment group control (P<0 .01) .Conclusion Early rehabilitation treatment can obviously improve the limb movement function and the daily living ability in the patients with small cerebral hemorrhage ,its mechanisms may be involved with the increase of circulating EPCs and VEGF .

Objectives To analyse the clinical feature,drug and surgical treatment and prognosis of rheumatic diseases associat- ed corneal melting.Design Retrospective case series.Participants 14 patients (22 eyes) with rheumatic diseases associated corneal melting.Methods The clinical data of patients were analysed,including history,visual acuity,slit-lamp microscopy examination,drug and surgical treatment and follow-up results.Main Outcome Measures The clinical feature,therapeutic effects and recurrence. Results The follow-up was 6～24 months.Rheumatic diseases associated corneal melting always firstly occurred from the upper corneal limbus.The marginal lesions of cornea included melting of the stroma,resulting in corneal thinning or even perforation,with neovascu- larization.The corneal lesions in 4 eyes were within 1 quadrant,10 eyes within 2 quadrants,6 eyes within 3 quadrants,and 2 eyes in 4 quadrants.Eight eyes of 5 cases,whose corneal lesions were thinner than 2/3 of normal corneal thickness,received 1% cyclosporine A and 0.5% heparin eyedrops.Among those,the lesions in 3 eyes of 2 cases progressed worse.Fourteen eyes of 9 cases,whose corneal lesions were thicker than 2/3 of normal corneal thickness,received marginal lamellar keratoplasty.Among those,recurrent signs were noted in 10 eyes of 6 cases.Conclusions Rheumatic diseases associated corneal melting has typical clinical features.Local cy- closporine A and heparin treatment are effective for patients in early stage.A high recurrent rate is found for patients in late stage re- ceived marginal lamellar keratoplasty.

Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Ventricular Premature Complexes ; therapy

Objective To discuss the clinical characteristics of thalamic hemorrhage,the correlative risk factor and the factors influencing prognosis.Method The clinical datas of 74 cases of thalamic hemorrhage and CT examination results were analyzed to find out the relationship between the clinical characteristics and the prognosis.Results The old age and hypertension were the main causes of thalamic hemorrhage.The mortality of thalamic hemorrhage that rupturing into ventricle was higher than that not rupturing into ventricle.Conclusions The old age and hypertension are the main causes of thalamic hemorrhage.The clinical manifestation of thalamic hemorrhage is variety.The prognosis is related weith age,blood pressure and the present of rupturing into ventricle.

Objective To analyze the clinical value of ovarian surgery in the treatment of primary ovarian mucinous carcinoma patients with fertility requirements.Methods 51 cases with fertility requirements with primary ovarian mucinous carcinoma who received surgical treatment were analyzed.The postoperative serum follicle stimulating hormone (FSH) and luteinizing hormone (LH),estradiol (E-2),anti Mullerian hormone (AMH) were deteced.The patients were followed up for 3 years,the survival rate and fertility were observed.Results Of 51 patients underwent surgery,postoperative 7d,14d,the FSH,LH levels in serum were higher than before operation (F=24.198,21.554,all P<0.05),E-2,AMH,PSV values were lower than before operation (F=24.407,35.028,11.064,all P<0.05).During the follow-up period to June 2015,there were 4 patients died of tumor recurrence,2 patients were lost to follow-up,45 patients survived,the survival rate was 88.23%.Of 45 patients survived,41 patients were normal menstrual cycle,menstruation,menstrual cycle 26-31 d,average (28.2±2.2)d;4 patients with irregular menstruation.Married women in the follow-up of 44 cases,including 28 patients with successful pregnancy,the pregnancy rate was 63.64%,there were 4 cases of spontaneous abortion,pregnancy in 3 patients because of abortion,pregnancy examination found 17 cases of abnormal pregnancy,4 cases of premature delivery.Conclusion In patients with primary ovarian mucinous carcinoma,it is feasible to use fertility preserving function,and the pregnancy rate is high,but the follow-up and monitoring should be strengthened to avoid the bad prognosis.

Objective To assess the vision-related quality of life(VRQL) in patients with age-related maculopathy (AMD) and the related factors in Shanghai. Methods The VRQL of 105 patiens with AMD and 105 without (the control group) in Shanghai was investigated via Chinese-version low vision quality of life questionnaire. Resutls The Cronbach ? coefficient of the questionnaire is 0.97 in the patients with AMD, whose scores of the various quality of life were significantly lower than those in the control group. The more the exudative defect happened, or the longer the disease course developed, the lower the sccores were. All the scores of the scales and the total questionnaire were significantly related to the better or worse monocular visual acuity. The better monocular visual acuity was the first independent risk factor affecting all aspects of VRQL in pateints with AMD. Conclusions Decreased visual acutiy may gradually impair the VRQL in patients with AMD. Chinese-version questionnaire of low vision quality of life can provide the general information of the VRQL in patients with AMD.