Chronic Disease ; Cone-Beam Computed Tomography ; Humans ; Periapical Abscess ; pathology ; Periapical Granuloma ; pathology ; Periapical Periodontitis ; diagnostic imaging ; pathology ; Problem Solving ; Prognosis ; Radicular Cyst ; diagnostic imaging ; pathology

Humans ; Pulpitis ; diagnosis

Objective To investigate the clinical efficacy of auricular plaster therapy plus scalp acupuncture in treating post-stroke depression.Methods One hundred and twenty-five patients with post-stroke depression were randomly allocated to group A of 42 cases, group B of 42 cases and group C of 41 cases. Group A received scalp acupuncture alone; group B, auricular plaster therapy alone; group C, auricular plaster therapy plus scalp acupuncture. The Hamilton Depression Scale (HAMD) score was recorded in the three groups before and after treatment. The clinical therapeutic effects were compared between the three groups. Results There was a statistically significant pre-/post-treatment difference in the HAMD score in the three groups after two months of treatment (P<0.01). There was a statistically significant difference in the HAMD score between group C and group A or B after one and two months of treatment (P<0.05). The total effective rate and marked efficacy rate were respectively 95.0% and 33.3% in group A, versus 88.1% and 35.7% in group B, and 92.7% and 56.1% in group C, and the marked efficacy rate in group C were significantly different from that in group A and group B (P<0.05). There is not a statistically significant difference in the total effective rate among the three groups (P>0.05).Conclusion Auricular plaster therapy plus scalp acupuncture is an effective way to treat post-stroke depression.

Objective:To establish an in vitro root canal model infected by Enterococcus faecalis and to observe the morphology, distribution and relative position of Enterococcus faecalis in infected root canals.Methods: Ten human healthy premolars extracted for orthodontic reasons were collected. Following sterilization , a total of 5 specimens were aseptically transferred to separate Eppendorf tubes containing 1. 5 mL brain-heart infusion broth (BHI) inoculated with 0.1 mL Enterococcus faecalis suspension that had been adjusted to Mcfarland 5, and were incubated at 37℃ for 21 days. The other 5 specimens were as controls. The roots of all specimens were then split into two halves along the mesiodistal axis. One half was processed with light microscopic ( Brown & Brenn stain) to check the bacteria in dentinal tubules, and the other was observed with SEM to investigate the bacterial status in infected root canals. Results: Enterococcus faecalis could penetrate into the dentinal tubules about 330-1 000μm. A dense bacterial aggregation composed of Enterococcus faecalis and amorphous matrix was observed in the apical third of the root canals, whereas Enterococcus faecalis were seen free-floating or planktonic in the crown and middle third of the root canals . No microorganisms were found in the root canals of the controls. Conclusion:Enterococcus faecalis could form bacterial biofilm on the root canal walls and penetrate into the dentinal tubules. The in vitro model designed was simple, and had good practicability to make a further comparative evaluation of various antimicrobial methods in the reduction of intracanal bacteria.

Clinical data of 277 pregnant women attended to nutrition clinic at Haidian District Maternity and Children's Hospital,Beijing were analyzed retrospectively,including 64 cases of gestational diabetes mellitus(GDM),85 cases of gestational impaired glucose tolerance(GIGT)and 128 normal women(84 normal fasting blood glucose and 44 abnormal fasting blood glucose but normal glucose challenge test,GCT).There was a higher proportion of pregnant women with family history of diabetes among those with abnormal GCT.as compared to those with normal GCT(P＜0.05).Mean age was significantly higher in women with GDM than that in normal ones(P＜0.05),but no statistically significant difference in it was found between women with GIGT and normal GCT(P＞0.05).Body weight in women with GIGT was higher both before pregnancy and at GCT than that in normal ones(P＜0.05).Serum levels of total cholesterol and triglyceride presented an increasing trend in pregnant women of varied groups since their first prenatal care visits(P＜0.05),higher in those with GDM and/or GIGT than those in normal ones(P＜0.01).

Objective:To establish an in vitro root canal model infected by Enterococcus faecalis and to observe the morphology,distribution and relative position of Enterococcus faecalis in infected root canals.Methods: Ten human healthy premolars extracted for orthodontic reasons were collected.Following sterilization,a total of 5 specimens were aseptically transferred to separate Eppendorf tubes containing 1.5 mL brain-heart infusion broth(BHI) inoculated with 0.1 mL Enterococcus faecalis suspension that had been adjusted to Mcfarland 5,and were incubated at 37 ℃ for 21 days.The other 5 specimens were as controls.The roots of all specimens were then split into two halves along the mesiodistal axis.One half was processed with light microscopic(Brown & Brenn stain) to check the bacteria in dentinal tubules,and the other was observed with SEM to investigate the bacterial status in infected root canals.Results: Enterococcus faecalis could penetrate into the dentinal tubules about 330-1 000 ?m.A dense bacterial aggregation composed of Enterococcus faecalis and amorphous matrix was observed in the apical third of the root canals,whereas Enterococcus faecalis were seen free-floating or planktonic in the crown and middle third of the root canals.No microorganisms were found in the root canals of the controls.Conclusion: Enterococcus faecalis could form bacterial biofilm on the root canal walls and penetrate into the dentinal tubules.The in vitro model designed was simple,and had good practicability to make a further comparative evaluation of various antimicrobial methods in the reduction of intracanal bacteria.

Objective:To investigate the physical morphology of the root canal taper in human maxillary and mandibular permanent first molars.Methods: Forty maxillary and 44 mandibular extracted human permanent first molars were investigated.The roots were dissected transversely from their apical foramen vertical to the long axis of the root,and 1-mm-thick serial sections were made.The root apex side of each section was observed with a stereomicroscope at 30? magnification.The maximum and minimum canal diameters were measured at 0,1,2,and 3 mm from the apical foramen.The physical canal taper in the apical section was calculated.Results: 47.5%-60.0% of the narrowest diameter of root canals were present at the foramen,and 25.0%~42.5% located at the level of 1 mm from apical foramen.Almost all physical configurations of root canals in 3 mm apical region showed a bottleneck-like taper.Canal dia-meters increased rapidly at 1-2 mm with the biggest taper,and the taper had a bigger variance(0.07-0.35).The ring of shrunken bottleneck was formed at the level of 1 mm from apical foramen.The taper of canal in maximal diameter was larger than the one in minimal diameter.Conclusion: The physical morphology of bottleneck-like taper in apical canal was helpful to locate the canal apical stop in root canal treatment.

Dental Pulp Cavity ; Dental Pulp Diseases ; therapy ; Humans ; Root Canal Preparation ; Root Canal Therapy

Acute Disease ; Anti-Bacterial Agents ; therapeutic use ; Chronic Disease ; Disinfectants ; therapeutic use ; Humans ; Periapical Abscess ; diagnosis ; therapy ; Periapical Granuloma ; diagnosis ; therapy ; Periapical Periodontitis ; classification ; diagnosis ; pathology ; therapy ; Problem Solving ; Prognosis ; Root Canal Irrigants ; therapeutic use ; Root Canal Therapy

Dental Pulp Diseases ; therapy ; Endodontics ; methods ; trends ; Humans