OBJECTIVETo study the compatibility between human adipose-derived mesenchymal stem cells and porcine bone scaffolds.

METHODSPorcine bone tissues were co-cultured with adipose-derived mesenchymal stem cells, and the complex was observed under scanning electron microscope. The viability and alkaline phosphatase (ALP) activity of the cells were examined with the cells co-cultured with human bone scaffold as the control.

RESULTSAt 4 and 10 days after the co-culture, the adipose-derived mesenchymal stem cells were observed to extend pseudopodia to adhere to the two scaffold materials. MTT assay showed that the cell proliferation on both of the materials increased with time, and the two cell complexes exhibited similar pattern of changes in ALP activity.

CONCLUSIONAs the seed cells, human adipose-derived mesenchymal stem cells exhibit good comparability with porcine bone scaffold, suggesting their potential of constructing tissue-engineered bone graft.

Adipose Tissue ; cytology ; Adult ; Alkaline Phosphatase ; metabolism ; Animals ; Bone and Bones ; cytology ; metabolism ; Cell Proliferation ; Coculture Techniques ; Humans ; Materials Testing ; Mesenchymal Stromal Cells ; cytology ; enzymology ; metabolism ; ultrastructure ; Microscopy, Electron, Scanning ; Swine ; Time Factors ; Tissue Scaffolds