China ; epidemiology ; Female ; Humans ; Papillomavirus Infections ; epidemiology ; prevention & control ; Papillomavirus Vaccines ; administration & dosage ; Uterine Cervical Neoplasms ; epidemiology ; prevention & control ; virology ; Vaccination

Cancer Vaccines ; therapeutic use ; China ; epidemiology ; Female ; Human papillomavirus 16 ; Human papillomavirus 18 ; Humans ; Neoplasms ; mortality ; prevention & control ; Papillomavirus Infections ; Primary Prevention ; Risk Factors ; Uterine Cervical Neoplasms ; prevention & control ; virology

Aim To study the effects of Ganoderma polysaccharide peptide (GLPP) on the production of nitric oxide (NO) in mice peritoneal macrophages induced by LPS and its mechanism. Methods The effects of GLPP on the NO releasing from mice peritoneal macrophages induced by LPS were detected by using Griess reagent and the expression of iNOS by GLPP in mice peritoneal macrophages was detected by immunohistochemical method.Result The production of NO was increased by GLPP.(25～200 mg?kg -1) ig for five days or by GLPP(3.125～200 mg?L -1) in vitro but the effects of LPS on the production of NO was not influenced significantly. The expression of iNOS was increased by GLPP(25～200 mg?kg -1) ig for five days or GLPP(3.125～200 mg?L -1) in vitro.Conclusion GPP in vivo or in vitro could increase the production of NO in mice peritoneal macrophages. It might take effects by enhancing the synthesis of iNOS.

Aim To study the protective effects of Ganoderma lucidum polysaccharides peptide(GLPP)on ECV304 from oxidative injury.Methods Cultured ECV304 were injured by oxygen free radicals derived from tBOOH.Various concentrations of GLPP(12.5,25,50,100 mg?L-1)were added into culture medium.The survival rate of cells was measured by MTT assay.The morphological change of cells and injury of mitochondria were examined under the light and electron microscopes.The percentage of apoptosis of ECV304,labeled with AnnexinV/PI,was measured by flow cytometry.Results GLPP(12.5,25,50,100 mg?L-1)could reduce oxidative injury induced by tBOOH in ECV304 cells.The survival rate of cells treated with GLPP increased.The light microscopic examination showed that the injured cells decreased in GLPP-treated groups.Under the electron microscope it was found that GLPP(50 mg?L-1,incubated for 24 h)could protect the organelle such as mitochondria from oxidative injury and cells from apoptosis by tBOOH.The result of flow cytometry showed that the total percentage of apoptosis in control,GLPP and injury treated group was 2.24%?0.43%,24?6.4%(P

AIM: To investigate the free radical scavenging activity of Ganoderma lucidum polysaccharides peptide (GLPP) on peritoneal macrophages in mice. METHODS: Alloxan and tert butylhydroperoxide (tBOOH) were used as an oxidant to injury peritoneal macrophages in vivo in mice or in vitro, respectively. 2', 7' dichlorodihydrofluorescein diacetate (DCHF DA) was used as fluorescent probe. The fluorescence from cells was observed under the laser confocal microscope. Time series scan of conforcal microscope was used to observe the changes of fluorescence by GLPP in mice peritoneal macrophages over time. RESULTS: The results of confocal microscopy showed that GLPP (100 mg?kg -1 , ig for 5 d ) lowed fluorescence in the mice macrophages injured by alloxan (75 mg?kg -1 iv). GLPP (10 mg?L -1 ) also lowed fluorescence in the mice macrophages injured by tBOOH ( 7.76 ?10 -5 mol?L -1 ) in vitro as well. Time series scan showed that GLPP (10 mg?L -1 ) lowed fluorescence in the mice macrophages at rest state or during the respiratory burst induced by PMA (50 nmol?L -1 ). CONCLUSION: GLPP shows antioxidant effects and might have free radical scavenging effects on peritoneal macrophages in mice.

OBJECTIVETo observe the effect on female stress urinary incontinence (SUI) treated with the pudendal nerve stimulation of electroacupuncture therapy at different frequencies.

METHODSSixty patients were divided into a group treated three times every week (group A) and a group treated twice every week (group B), 30 cases in each one. The pudendal nerve stimulation of electroacupuncture therapy was adopted at Disixue (four acupoints on sacral region), 60 min in each time in the two groups, treated three times a week or twice a week respectively. SUI severity and the score of life quality were observed before and after 12 treatments separately. The efficacy was evaluated in the two groups.

RESULTSAt the end of treatment, SUI severity was relieved and the score of life quality was improved in the two groups (all P<0.01). The results in group A were superior to group B [0 (0-4.3) vs 4 (0-5), P<0.01]. At the end of treatment, the total effective rate in group A was 90.0% (27/30) and was 76.7% (23/30) in group B; the efficacy in group A was better than that in group B (P<0.05).

CONCLUSIONThe pudendal nerve stimulation of electroacupuncture therapy achieves the definite efficacy on female SUI and the results in the treatment of three times a week are superior to those in the treatment of twice a week.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Electroacupuncture ; Female ; Humans ; Middle Aged ; Quality of Life ; Treatment Outcome ; Urinary Incontinence, Stress ; psychology ; therapy

Acute Disease ; Case-Control Studies ; Cross-Over Studies ; Hand Injuries ; epidemiology ; Humans ; Occupational Diseases ; epidemiology ; Prognosis

Brain metastasis is among the most common complications of non-small celllung cancer (NSCLC) and may obviously influence the survival and quality of life in the NSCLC patients. Recently, the development in traditional treatments, the widely-used micro-molecular targeted drugs, and the combined treatment have improved the overall survival and the quality of life of these patients. In addition, the exploration of new therapeutic targets and cognitive function protective methods for current treatments also deserves at-tention. This review emphasizes the relevant clinical and basic problems and the therapeutic strategies in NSCLC with brain metastases.

[Objective]To investigate the feasibility and merits of the Smith-Peterson osteotomy for the treatment of degenerative lumbar scoliosis. [Methods]Twenty-five patients(11 males,14 females)with degenerative lumbar scoliosis malformations underwent operations from May 2004 to October 2007.The mean average age were 67 years (range,56~77 years).All the patients underwent more than three segments Smith-Peterson osteotomy and lumbar pedicle screw fixation to have the reconstruction of spinal coronal and sagittal plane balance.[Results]The mean duration of surgery were 200 mins(range,150~320 mins),and the average intraoperative blood loss were 1000 ml (range,600~2 000 ml).There were two cases of postoperative cerebrospinal fluid leakage,which were alleviated by pulling drainage tube and oppressing the wound.Postoperative pain and numbness in both lower extremities were aggravated in 2 cases,one of which was recovered completely 3 weeks later,and another 12 weeks later.The mean clinical follow-up were 36 months(range,24~60 months).JOA 29 score was recorded to evaluate the relief of the symptoms.The good-to-excellent rate was 88.2% and the poor rate was 0%.The coronal Cobb's angle was improved from the preoperative average of 23? (range,15?~ 40?) to the postoperative average of 12? (range,8?~ 20?).The sagittal Cobb's angle was improved from the preoperative average of 0 ?(range,-15?~ 15?) to the postoperative average of 33?(range,25?~ 40?).[Conclusion]Smith-Peterson osteotomy is an effective method for the decompression of lumbar spinal stenosis,and is helpful to correct the sagittal deformity of the degenerative lumbar scoliosis.It also has relatively small interference to nerve in the operation.

The effects of electro-and chemo-stimulating (ES, CS) supraoptic nucleus (SON) on analgesia and electroacupuncture (EA) analgesia were observed using potassium inotophoresis induced tail-flick in rats. The results showed that: different electrical current (12.5 ~ 50 ?A) stimulating SON elevated the PT (P