The anatomical and functional details of the anterior cruciate ligament (ACL) of the knee joint were studied on 55 normal cadaver knees of adults.ACL is made up of multiple fascicles, the basic unit of which is collagen. It is attached to the medial surface of the lateral femoral condyle and front of the anterior tibial spine. ACL is narrow in the middle and fans out at the attachments. The fascicles of ACL have been summarily divided into two groups: the anterior medial portion (AMP) and the posterior lateral portion(PLP). The tension of AMP is different from that of PLP during movement of the knee. ACL restricts the anterior drawer sign, and the internal and external rotation of the knee. AMP acts with the knee in flexion and PLP in extension. ACL restricts hyperextension, too. The function of ACL is related to its morpholcgical structure and the anatomy of the attachments. Rupture of ACL may result in instability of the knee, and injuries to the different components of ACL may produce different signs.

By comparing three cultivation techniques of Astragalus mongholicus, i. e. direct seeding, furrow erectplanting and furrow flat planting, it was found that furrow flat planting is the optimum technique. Its yield onplain ground was 1. 26kg/m' and 0. 96kg/m_2 on mountainous area- Root of the plant was longer, thicker andheavier than that produced hy other cultivation techniqlles. Its input/output ratio was l s l2' 94 on plaingrounfl and l: 9. 95 on n1ollntainous area. Therefore,it can be considered as a scientifically justifiable,economically technique for the cultivation of A. mongholicus on both plain ground and mountainous regions.

Objective To investigate the relationship between pulse pressure (PP) and coronary stenosis in hypertension patients. Methods Parameters of blood pressure, clinical features and coronary angiographic findings were analyzed retrospectively in 336 patients with essential hypertension under standard antihypertensive treatment. Results ①coronary stenosis were increasing with PP raising( P

OBJECTIVE:To provide reference for the construction and management of hospital clinical drug trial institution. METHODS: The practice of our hospital in the construction and management of hospital clinical drug trial institution in accordance with the Good Clinical Practice and the Trial Provisions for Drug Clinical Trial Institution Qualification was summarized. RESULTS: Hospital attached great importance by taking effective measures,strengthening the construction of software and hardware and emphasizing reforming was the key to pass the qualification confirmation,and the key points in the management are to tighten control on the clinical trial process and establish complete management regulations. CONCLUSION: The construction and management of hospital clinical drug trial institution is conducive to the improvement of clinical trial level and it serves as a scientific,accurate and reliable basis for the evaluation and marketing approval of new drugs.

Objective:To promote hospital medicalmetrology and quality control management. by using information management mode.Methods: Through the analysis of our hospital medical equipment measurement and quality control management present situation, put forward the necessity of metrology and quality control management of Xinxi sanitary equipment,introduces the application situation of our hospital dynamic health equipment metrology and quality control management system.Results: The medical equipment metrology and quality control management system perfect the medical equipment measurement and quality control of management process, improve the qualified rate of equipment.Conclusion:Application of sanitary equipment measurement and quality control management platform to improve the delivery rate of equipment, ensure the equipment unqualified clinical use.

[ ABSTRACT] AIM: To observe the effect of microRNA-19a ( miR-19a) on the lipid catabolism of hepatocyte LO2, and to explore the potential mechanism.METHODS: miR-19a was over-expressed or silenced by transfection of miR-19a mimics or miR-19a inhibitor into LO2 cells, then the mRNA level of miR-19a was detected by real-time PCR.The potential target of miR-19a was found by the method of bioinformatics through internet website.The effect of miR-19a on the 3’ UTR of peroxisome proliferator-activated receptorα(PPARα) was measured by dual luciferase reporter assay, and the protein level of PPARαand its 2 major downstream rate-limiting enzymes involved in lipid catabolism, acyl-coenzyme a dehydrogenase (ACADM) and carnitine palmitoyltransferase 1A ( CPT1A), were detected by Western blotting.Mean-while, the effect of miR-19a on the generation of ketone body was measured by beta-hydroxybutyric acid (β-OHB) detec-tion assay.RESULTS:The mRNA level of miR-19a was dramatically elevated by the transfection of miR-19a mimics, and sharply decreased by the transfection of miR-19a inhibitor (P<0.05).PPARαwas found as a potential target of miR-19a, and dual luciferase reporter assay and Western blotting confirmed the regulatory effect of miR-19a on the expression of PPARα, with the protein level changes of ACADM and CPT1A.miR-19a mimics down-regulated, while miR-19a inhibitor up-regulated the concentration ofβ-OHB in LO2 cells (P<0.05).CONCLUSION:miR-19a regulates the lipid catabo-lism of hepatocytes by targeting the PPARαand its 2 downstream rate-limiting enzymes.

By setting up the medical affairs office and updating the hardware equipment,we improved the working mode of the triage nurses and optimized the triage procedure in the outpatient department,which resulted in a better order,a more convenient access to medical care and increased satisfaction of patients with outpatient services.

Background Rosuvastatin has been unanimously recognized as an very highly efficacious statin. Rosuvastatin significantly reduced microalbuminuria and its powerful anti-inflammatory effect confer anti-atherosclerosis in patients with essential hypertension. Objective To study the influence of rosuvastatin on microalbuminuria and arteriosclerosis plaque of carotid and coronary arteries in hypertensive patients. Methods Seventy-six hypertensive patients were randomly to receive conventional anti-hypertensive drugs (amlodipine 5 mg/d and telmisartan 80 mg/d,n=37) or rosuvastatin (10 mg QN) on the top of conventional anti-hypertensive drugs (n=39). The blood pressure,levels of microalbuminuria,echocardiography and helical computerized tomography were examined before and 8 months after treatment in all patients. Results After 8 months of treatment,SBP and DBP were decreased in both group [conventional treatment from (166.3?11.2)/(92.4?8.2) to (133.6?9.8)/(85.5?6.1)mmHg,combined group from (165.6?10.5)/(91.5?6.7) to (128.1?9.2)/(81.1?5.9)mmHg]. Combined treatment significantly reduced the levels of microalbuminuria [(31.6?21.8) to (23.2?19.8)mg/g,P

Objective To observe the changes of plasma Von Willebrand factor(vWF) content in chronic obstructive pulmonary disease(COPD) and late lung cancer(LLC) patients with Qi deficiency.Methods The changes of vWF content in 26 COPD patients with Qi deficiency and in 15 LLC patients with Qi deficiency were observed before and after treatment,and were compared with those in 30 healthy volunteers.Results Plasma vWF content before treatment was increased in COPD and LLC patients with Qi deficiency(P0.05).Conclusion Abnormal platelet activation exists in COPD and LLC patients with Qi deficiency,the function of vascular endothelial cells decreases,and the blood is in hypercoagulable state,which accords with the traditional Chinese medical theory of Qi failing in promoting blood circulation.

Objective To explore the effects of Sinomenine (Sin) on phenotype transdifferentiation in renal tubular epithelial cells. Methods Mice primary tubular epithelial cells and cell line (MCT) were cultured in vitro. Model group cells were stimulated by IL-1?(10 ng/ml). The cells of Sin group were co-incubated with Sin(10, 100 ,500?mol/L) simultaneously. The cells without IL-1?and Sin treatment were regarded as control group. The protein expression of?-smooth muscle actin (SMA) and Vimentin was assessed by immunohistochemistry and Western blotting. The mRNA expression of?-SMA and Vimentin was examined by RT-PCR. Secretion of matrix metalloproteinases was determined by zymography. Results The mRNA expression and protein level of?-SMA and Vimentin, and the enzyme activity of MMP-2 and MMP-9 in tubular epithelial cells stimulated with IL-1?were significantly higher than those in the controls. Sinomenine significantly reduced the expression levels of protein and mRNA of?-SMA and Vimentin. At the same time, the MMP-2 and MMP-9 activities were reduced markedly. Conclusion Sinomenine can decrease the transdifferentiation in renal tubular epithelial cells stimulated by inflammatory factor IL-1?and prevent renal interstitial fibrosis.