Humans ; Psychological Tests ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; psychology

Synergistic antitumor effects of HB (berberine alpha-hydroxy-beta-decanoylethyl sulfonate, houttuyn berberine) with HCPT (hydroxycamptothecine), and its correlative mechanism were studied in vitro. MTT assay was employed to determine the cytotoxicity of HB combined with HCPT in tumor cells culture in vitro, IC50 and combination index (CI value) were used to evaluate the synergistic effects. The supercoiled DNA relaxation mediated by topoisomerase I & II was measured by agarose gel electrophoresis assay, and influence of HB was detected. The results showed that HB could inhibit the proliferation of tumor cells (SGC-7901, SW1116 and SW480) in vitro, and the inhibition ratio was increased, IC50 was reduced when combining with HCPT. CI value of the two drugs was less than 1 in HepG2, SW480, SGC-7901 and SW1116 cells. The lowest value was 0.447, 0.626, 0.161 and 0.178 in these tumor cells, respectively, further indicating HB has synergistic action with HCPT on suppressing tumor proliferation. The agarose gel electrophoresis assay showed HB can inhibit topoisomerase I & II activity of SW480 cells at the concentration of 2.0-8.0 mg x L(-1). HCPT is a typical inhibitor of topoisomerase I , the synergistic action between HCPT and HB on suppressing tumor proliferation is perhaps related to the congenerous inhibition of topoisomerase.

BACKGROUND:Studies have shown that the changes of extracellular matrix in degenerative intervertebral disc tissues mainly present as the decrease of col agen type Ⅱ and proteoglycan contents and the increase of col agen type Ⅰ content. OBJECTIVE:To explore the effect of adeno-associated virus-mediated bone morphogenetic protein 2 gene on nucleus pulposus Ⅰ and Ⅱ col agen levels in rabbit degenerative intervertebral disc tissues. METHODS:L 2-3 , L 3-4 , L 4-5 and L 5-6 lumbar discs of 12 New Zealand white rabbits were punctured to establish interverbral disc degeneration model. Subsequently, 12 rabbits were randomly divided into three groups, four rabbits in each group. The intervertebral discs in the adeno-associated virus-mediated bone morphogenetic protein 2 group were injected with the adeno-associated virus-mediated bone morphogenetic protein 2 gene, the intervertebral discs in the adeno-associated virus group were injected with adeno-associated virus only, while the discs in the normal saline group were injected with normal saline. Al rabbits were sacrificed after injected for 8 weeks, and the L 2-3 , L 3-4 , L 4-5 and L 5-6 lumbar discs of each rabbit were col ected, paraffin-embedded and sliced. The histological changes of nucleus pulposus were observed with hematoxylin-eosin staining, and the immunohistochemistry was used to detect the col agen type Ⅰ and Ⅱ expressions in nucleus pulposus. Semi-quantitative analysis was performed. RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that the nucleus pulposus in the intervertebral disc tissues was less in the adeno-associated virus-mediated bone morphogenetic protein 2 group, the nucleus pulposus was in single or clustered distribution with clear nucleus structure and without fibrous tissue fil ing. The tissue structures of nucleus pulposus were the same in the adeno-associated virus group and normal saline group, the cellnumber in nucleus pulposus was smal , the nucleus pulposus was shrunken and shriveled, and the cells were fil ed with fibrous tissue and arranged disorderly. Immunohistochemistry staining showed the expression of col age type Ⅰ in the intervertebral disc nucleus pulposus of adeno-associated virus-mediated bone morphogenetic protein 2 group was higher than that of the adeno-associated virus group and normal saline group (P<0.05);the expression of col age typeⅠ in the intervertebral disc nucleus pulposus of adeno-associated virus-mediated bone morphogenetic protein 2 group was lower than that of the adeno-associated virus group and normal saline group (P<0.05). The results indicate that adeno-associated virus-mediated bone morphogenetic protein 2 can inhibit the expression of col agen type Ⅰ in the intervertebral disc nucleus pulposus, promote the expression of col agen type Ⅱ. Maintaining the content of col agen in intervertebral disc can keep the histological structure and morphology of intervertebral disc, stabilize the environment for nucleus pulposus cellgrowth, and delay the intervertebral disc degeneration.

Biomedical Research ; Drugs, Chinese Herbal ; therapeutic use ; Eczema ; drug therapy ; Humans ; Integrative Medicine

Objective To evaluate the feasibility of combined application of helicobacter pylori stool antigen(HpSA)-and traditional serology in epidemiologic study of helicobacter pylori(Hp) infection in children. Methods Hp serum IgG antibodies were examined among 480 healthy children. Heticobacter pylori stool antigen(HpSA) were measured among children who were seropositive. Hp status was defined as positive when stool antigen test was positive, Hp status was defined as negative when seronegative. A case - control study was conducted to identify risk factors of Hp infection in preschool children. Results Among 480 preschool children, 172 (35 8 %) were sero- positive, 64(13.3 %) with positive result of HpSA test were diagnosed as current Hp infection. Univariate factor analysis demonstrated that Hp infection clustered in families and Hp infection was often acquired in childhood by oral-oral and fecal - oral person to person transmission. Conclusions HpSA and serology screening are two nomnvasive tests, simple not costly, effective and accurate. The combination of these 2 noninvasive methods can be applied in epidemiologic study of Hp infection in childrenJAppl Clin Pediatr,2004,19(11):948-950

Hemangioma ; Humans ; Vascular Malformations

Objective To study the effect of action‐oriented health education in the patients with permanent urinary bladder fistula .Methods From January 2012 to March 2015 ,78 patients with permanent urinary bladder fistula were randomly divided into two groups ,39 cases in the control group were given routine health education ;39 cases in the in the study group were given action‐oriented health education ,at the 12‐month follow‐up ,the QOLI and self‐care ability of the patients were used to evaluate life self‐care abilityof patient quality .Results After 12 months ,the self‐care skills ,sense of responsibility ,health knowledge score and total score of the study group were higher than those of the control group (P< 0 .05) .The self‐care level of the study group was 94 .87% ,5 .13% and 0% respectively ,the control group were 23 .08% ,58 .97% ,17 .95% ,there was significant difference between the two groups(P< 0 .05) .The complication rate(17 .95% ) in the study group was significantly lower than that in the control group(61 .54% ) ,and the difference was statistically significant (P< 0 .05) .The psychological function ,physical function ,social function and total score of the study group were higher than those of the control group(P<0 .05) .There was no statistically signifi‐cant difference between the two groups in material life scoring (P>0 .05) .Conclusion Action‐oriented health education method has a positive effect on improving self‐care ability and quality of life ,and reducing the incidence of complications in patients with perma‐nent bladder stoma .

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Earthquakes ; Female ; Humans ; Male ; Middle Aged ; Pain Management ; Young Adult

Objective To explore the factors affecting the circadian rhythm of blood pressure in treated hypertensive patients. Methods Based on the clinical data, ambulatory blood pressure, 24-hour mean blood pressure (24hMBP) and circadian rhythm, 125 consecutive patients receiving antihypertensive treatment were divided into the following groups: normal 24hMBP and dipper type (type I), abnormal 24hMBP and dipper type(type E), abnormal 24hMBP and non-dipper type (type I), and normal 24hMBP and non-dipper type (type IT). The clinical data and ambulatory blood pressure of the latter three groups were compared. Non-conditional Logistic regression was used to analyze the factors for ambulatory blood pressure. Results Ninety patients had abnormal 24hMBP (72. 0%), and 106 (84. 8%) had abnormal circadian rhythm. Among these cases, 2 (1.6%) patients had type I, 17 (13.6%) had type TJ, 73 (58.4%) had type I, and 33 (26.4%) had type IT. The incidence of kidney disease in patients with abnormal 24hMBP was significantly higher than those with normal 24hMBP (P< 0. 05). The incidence of diabetes mellitus in patients with abnormal circadian rhythm (non-dipper type) was higher than those with normal circadian rhythm (dipper type), with the highest incidence seen in patients of type IT (P<0. 05); the rates of calcium channel blocker (CCB) use and a daily morning dose administration in type TV patients were higher than those in type II and type III patients (P<0. 05). Type IT group had the most severe abnormal ambulatory blood pressure among the latter three groups. Logistic regression analysis showed that complication with renal diseases (OR=0. 301, 95%CI:0. 124-0. 729, P = 0. 008), use of CCB (OR = 2. 191,95%CI: 0. 967-4. 966,P = 0. 048), and administration of a morning dose (OR = 2. 384,95% CI: 1. 017-5. 591,P = 0. 046) were the factors of ambulatory blood pressure. Conclusion Abnormal circadian rhythm of blood pressure is high in patients receiving antihypertensive treatment. Complication with kidney diseases, use of CCB, and a daily morning dose are the factors for ambulatory blood pressure, indicating it is be reasonable to analyze factors for circadian rhythm by combining the 24hMBP and the components of circadian rhythm.

Female ; Humans ; Hyperkeratosis, Epidermolytic ; pathology ; Infant, Newborn