OBJECTIVE: To validate the method for microbial limit test of Niuhuang jiedu tablet.METHODS: The recovery rates of 5 kinds of test organisms including E.coli treated by Niuhuang jiedu tablets were determined by routine method,culture medium dilution method,centrifugation and membrane-filter method,respectively.RESULTS: The centrifugation and membrane-filter method was suitable for determination of the bacterial number of Niuhuang jiedu tablets,while routine method was applicable for determination of bacterial numbers of mold and yeast and test of control bacteria in the microbial limit test.CONCLUSION: The contaminated bacteria in Niuhuang jiedu tablet can be detected accurately by the method established in this study.

Objective In order to provide a large quantity of retinal pigment epithelial cells (RPE cells) in vitro, we want to estab-lish method for culturing pig RPE cells. Methods RPE cells were separated with trypsin and cultured in vitro. The cells were identified by immunohistochemical staining with anti-human keratin and transmission electron microscope (TEM). Results Cultured RPE cells gradually presented transparent and fusiform shape. Immunohistochemical staining demonstrated that the cells were stained by anti-human keratin, and the cultured cells showed typical ultrastructure of RPE cells. Conclusion The cultured cells may be the foundation of pig RPE cells.

Objective To investigate the effect of Mongolian medicine naru-3 on the apoptosis of mouse hepatic cells induced by radiation. Methods Kunming mice were radiated with 60Coγ-irradiation, the 30-day survival rate of mice and the average life span of dead mice post-irradiation were observed. The apoptosis of hepatic cells from irradiatied mice was detected by FACS and TUNEA expression level of p53 was examined. Results Administration of the medicine resulted in increase of 30-day survival rate and prolongation of average life span of the dead mice. The apoptosis rate of spleen cells from mice treated with Mongolian medicine naru-3 decreased significantly and the ex pression level of p53 was also inhibited significantly. Conclusion The results indicated that Mongolian medicine naru-3 had radio protective function and its mechanism might be related to the suppression of apoptosis of radiationsensitive cells.

Objective To explore psychological defense mechanism and its relationship with personality in patients with hysteria(HyS). Method Both HyS patients (n=30) and normal control(NC, n=66) comple-ted Defense Style Questionnaire (DSQ) and Eysenck Personality Questionnaire(EPQ) . Results The scores of projection, isolation, somatization and the factor scores of immature defenses were significantly higher in HyS pa-tients(3.64±1.45, 5.63±2.29,5.33±2.36 and 4.74±1.27, respectively) than those in NC [(2.61±1.09,4.33±2.02, 3.86±1.60 and 3.98±0.91, respectively) (all P<0.01)]. The scores of undoing, idealization, pseudo-altruism and the factor scores of neurotic defenses were also significantly higher in HyS patients compared with NC(P<0.01). The scores of suppression, humor and the factor scores of mature defenses and the lie were found lower significantly in HyS patients compared with NC (P<0.05). The scores of the neuroticism, psychoticism and lie subscale of EPQ in HyS patients were significantly higher than that of NC(P<0.01). The scores of extro-vert subscale were not found difference significantly between HyS patients and NC. The results of correlation analy-sis showed that the scores of the psychoticism subscale of EPQ in HyS patients correlated negatively with the factor scores of mature mechanism and the scores of suppression. Conclusion The HyS patients were more likely than the normal control to endorse the immature mechanism and neurotic defense and less likely to endorse the mature mechanism, which related with unhealthy personality, especially the psychoticism.

Objective To study effect of positive liver soup on serum leptin (LEP), adiponectin (ADP) and insulin resistance (IR) in patients with hepatitis B cirrhosis.Methods 68 cases of hepatitis B patients with cirrhosis according to randomized divided into the control group (34 cases) and treatment group (34 cases).Oral treatment administered to the control group (Inosine Tablets and vitamin C),treatment group in the control group based on liver soup treatment, treatment for 3 months, serum leptin (LEP),adiponectin (ADP) and insulin resistance (IR) index before and after treatment were detected.Results Before treatment, there was no significant difference between ADP and LEP in the control group and the treatment group.After 3 months of treatment, there was no significant difference between ADP and LEP level in control group, while the level of ADP and LEP in treatment group were significantly decreased (P<0.05), and were significantly lower than that in control group (P<0.05).There was no significant difference in FPG, fasting insulin level and IRS index between the control group and the treatment group before treatment.After 3 months of treatment, the control group FPG, fasting insulin level and IRS index were not significantly different.FPG, fasting insulin and IRS index in the treatment group were significantly decreased (P<0.05), and were significantly lower than those in the control group (P<0.05).Conclusion Positive liver soup can reduce serum LEP, ADP level, and improve IR in treatment of hepatitis B liver cirrhosis.

15℃ . Conclusion The changes of density of different strains of Escherichia coli in the undernourished water samples closely associated with the strain ' characteristics, environment temperatures and preserving time, showed significantly differences.

AIM: To evaluate the value of urethra perfusion using 0.5% povidone iodine liquid (PVP-I) in treatment of patients with chronic urethritis and prostatitis. METHODS: Outpatients with chronic urethritis and prostatitis were assigned to two groups randomly. 38 cases in treatment group were received urethra perfusion with 0.5 % PVP-I liquid plus minomycin, po, for two weeks. 42 cases in control group were received minomycin only. RESULTS: The rates of cure and total efficacy were better in treatment group than in control group (P

Objective To comprehend the etiology and the clinical characteristics of hypereosinophilia.Methods The clinical data of 53 patients with hypereosinophilia admitted to Peking Union Medical College Hospital from November 1992 to February 2005 were analyzed.Results The causes of reactive hypereosinophilia included allergic diseases,infections,connective tissue diseases,malignant diseases,lung diseases,eosinophilic gastroenteritis.idiopathic hypereosinophilic syndrome had longer course of disease;more organ involved than reactive causes.The incidence of lymph node enlargement,spleen enlargement and thrombus was higher than the reactive.IHES had higher level of white blood cell count,absolute eosinophilic count,IgG and CRP.Conclusion The etiology and clinical characteristics of hypereosinophilia are various,so the differential diagnosis of hypereosinophilia is very important.

This review snmmarized and estimated the management of cataract blindness in China recently,and introduced the purport of clinic medical economics.Based on it,it was proposed that our service system of cataract blindness could use the experiences of other countries as reference and engaged in more investigations in search of Chinese way for cataract blindness.

Objective To evaluate the feasibility of combined application of helicobacter pylori stool antigen(HpSA)-and traditional serology in epidemiologic study of helicobacter pylori(Hp) infection in children. Methods Hp serum IgG antibodies were examined among 480 healthy children. Heticobacter pylori stool antigen(HpSA) were measured among children who were seropositive. Hp status was defined as positive when stool antigen test was positive, Hp status was defined as negative when seronegative. A case - control study was conducted to identify risk factors of Hp infection in preschool children. Results Among 480 preschool children, 172 (35 8 %) were sero- positive, 64(13.3 %) with positive result of HpSA test were diagnosed as current Hp infection. Univariate factor analysis demonstrated that Hp infection clustered in families and Hp infection was often acquired in childhood by oral-oral and fecal - oral person to person transmission. Conclusions HpSA and serology screening are two nomnvasive tests, simple not costly, effective and accurate. The combination of these 2 noninvasive methods can be applied in epidemiologic study of Hp infection in childrenJAppl Clin Pediatr,2004,19(11):948-950