Objective To study the relationship between the therapeutic principle and prescription of traditional Chinese medicine and the experimental pharmacologic effects in relation to the treatment of eczema. Methods Murine DNFB allergic contact dermatitis was used as an animal model, and was treated with 4 traditional Chinese medicinal compounds which were composed of Chinese materia medica with activities to suppress type Ⅳ allergic reaction. Results The results showed that the effect of compound Ⅲ composed of Paeoniae obovata Maxim(赤芍), Fructus Gardeniae(栀子), Herba Schizonepetae(荆芥), Herba Spirodelae(浮萍) and Poria(茯苓), possessing multiple potencies of cooling and promoting blood, clearing heat, expelling wind and eliminating dampness, was strongest among the compounds tested, and the compound up regulated the serum level of calcitonin gene related peptide which was lowered in the mice with DNFB induced dermatitis. Conclusion It is suggested that the compounds with suppressive effect on type Ⅳ allergic reaction might improve the therapeutic effect in clinical practice.

OBJECTIVE: To establish the UPLC fingerprint of Gardeniae Fructus for providing reference for effective quality control. METHODS: The samples were analyzed on a Waters ACQUITY UPLC BEH C18(2.1 mm × 50 mm, 1.7 μm) column maintained at 30℃ and eluted with methanol and water containing 0.02% phosphoric acid as mobile phases in a linear gradient mode. The flow rate was set at 0.4 mL·min-1 and the injection volume was 0.2 μL. The detection wavelengths were set at 238 nm (0-5.4 min) and 440 nm (5.4-10 min). RESULTS: The fingerprints of 16 batches of samples were analyzed with similarity evaluation, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The common mode of the fingerprint was established with 24 common peaks, and four of them were identified, which were genipin 1-gentiobioside (4), gardenoside (6), crocin I (15), and crocin II (17). The similarity degrees of the 16 batches of samples were between 0.986 0 and 0.995 0.The samples were divided into two groups, ZheJiang (S1-S6) and other areas (S7-S16), analyzed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). Eleven significantly different components were found, including genipin1-gentiobioside, gardenoside, crocin I, crocin II and some others. CONCLUSION: The establishment of UPLC fingerprint of Gardeniae Fructus from different producing areas and the application of chemical pattern recognition can provide more comprehensive references for the quality control and evaluation of genuine Gardeniae Fructus from Zhejiang Province.

The new rural cooperative medical system(NRCMS)is a significant breakthrough for people's wellbeiug as emphasized by the government in the new century.To better the system and further the social and economic development,the instant reimbursement practice came into being under the NCRMS.The article introduced the background and implementation of the practice at provincial and municipal level at designated medical institutions in Fujian province.It also analyzed the benefits and initial outcomes of the practice for peasants at large,and proposed remedies for setbacks found in the practice of NRCMS.

Objective To study the X-ray and CT features of costal eosinophilic granuloma for a better understanding.Methods Eight patients with costal eosinophilic granuloma proved by surgery or biopsy were analyzed retrospectively.All patients had X-ray plain film, 6 patients had CT examination,including a case of enhanced CT scan.Results All 8 lesions were solitary.Six lesions were in the anterior rib and 2 in the posterior rib.On X-ray, all case showed single cavity and oval lesion with clear boundary.On CT images, 5 lesions demonstrated expansile destruction of bone with cortical bone thinning, and 3 were osteolystic destruction with soft tissue mass around.On the patient with enhanced CT scan, the lesions showed a moderate and uniform enhancement.Conclusion The X-ray and CT findings of costal eosinophilic granuloma are characteristic.

Purpose:To study the expression and clinical significance of p27 in esophageal carcinoma. Methods:Immunohistochemical staining was used to determine the expression of p27 in 32 cases of normal esophageal issues and 88 cases of esophageal carcinoma.Results:The positive incidences of P27 expression in esophageal carcinoma was 40.9% while in normal esophageal issues it was 75.0%. The expression of p27 was correlated to degree of differentiation of carcinoma and lymph node metastasis, but not to length, depth of invasion and TNM stage. The p27 negative group was seen to have the worst survival rate among all groups.Conclusions:The results suggest that p27 gene plays an important role in carcinogenesis and progress of esophageal carcinoma. Expression of P27 may prove to be valuable in prognosis assessment of esophageal carcinoma.

Objective To explore the mechanism of siRNA targeted hTERT gene to inhibit bladder cancer T24 cell growth by decreasing c-myc and TGF-?1 expression. Methods shRNA-hTERT-pTZU6+1 vectors were constructed by RNAi-DNA vector technique, then the vectors were transfected into bladder cancer T24 cells,and the most effective vector and its optimal concentration were screened using RT-PCR to detect hTERT expression in T24 cells.The T24 cell growth, the alternative of cell phase,the expression of hTERT,c-myc and TGF-?1 were detected by flow cytometry,RT-PCR and immunohistochemistry. Results Three shRNA-hTERT-pTZU6+1 vectors were successfully constructed.The most effective vector was ph2-shRNA vector,and its optimal concentration was 1.0 ?g.This vector decreased the cell growth and the cell number of S phase from 65.2% to 38.6%,increased the cell number of G0/G1 phase from 32.0% to 57.9%,and attenuated both mRNA and protein expressions of hTERT,c-myc and TGF-?1 in T24 cells. Conclusions Targeted hTERT gene with siRNA may inhibit the cell proliferation of bladder cancer;down-regulating hTERT expression by attenuating the expression of c-myc and TGF-?1 is probably involved in the mechanism.

In order to study the possible role of soluble interleukin-2 receptor (slL-2R) in the pathogenesis of vitiligo, we measured the levels of slL-2R in the sera and the tissue fluids from skin lesions and uninvolved skin from 41 patients with vitiligo, using the method of sandwich ELISA with monoclonal and polyclonal antibodies. The results showed that the overall level of serum sIL-2R from patients with vitiligo was significantly higher, than that from normal control group (P0.05). The level of serum sIL-2R from patients with vitiligo in progressive stage was significantly higher than that in stable stage (P

Objective To investigate the relationship between procoagulation of HFGL 2 and abnormality of coagulation in patients with systemic lupus erythematosus (SLE) by detecting the expression of HFGL2/fibroleukin in peripheral blood mononuclear cell(PBMC) of SLE patients. Methods A polyclonal antibody against HFGL2 was applied to detecting the expression of HFGL2 protein in 32 SLE patients and 15 healthy volunteers by immunohistochemistry. Semi-quantitative measurement of HFGL2 expression in the blood specimen was done with high multiple image analytical system(HMIAS). Results The expression of HFGL2 in PBMC from 23 active SLE patients was significantly higher than that in the controls, which showed a positive correlation with the disease activity. Conclusion The expression of HFGL2 in PBMC is probably correlated with the pathogenesis and disease activity of SLE.

AIM: To investigate the transcription regulation of the promoter of human telomerase reverse transcriptase (hTERT) by transcription factors c-myc and [STBX]mad1. METHODS: The various plasmids including wild type hTERT (Tw) or mutant type hTERT (Td) which both harboring luciferase gene, the expression plasmids of c-myc and [STBX]mad1, and their control vectors were constructed. The plasmids were co-trans fected into bladder cancer cell lines T24, EJ and control cells COS-7 or fibrocytes by DOTAP liposome in various combining manner, respectively. The reporter gene luciferase activities in various groups were measured 48 h after transfection. RESULTS: The luciferase activities in T24 and EJ cells treated with Tw were much higher than that in COS-7 and fibrocytes cells treated with Tw, as well as higher than that in T24 and EJ cells treated with Td, respectively. In bladder cancer T24 and EJ cells, transcription factor c-myc and [STBX]mad1 positively and negatively regulated Tw expression in a dose-dependent manner. However, the effects of c-myc and [STBX]mad1 on Td were completely opposite to Tw. Combined with c-myc and [STBX]mad1, down-regulation of Tw expression was observed. CONCLUSION: c-myc and [STBX]mad1 regulates the transcriptional activity of hTERT promoter in bladder cancer cells, and the effects might highly depend on the conservative E-box sequence CACGTG.

Objective To explore the feasibility of laparoscopic subtotal cholecystectomy without using titanium clips.Methods Laparoscopic subtotal cholecystectomy was performed without using titanium clips on 48 patients,who had serious adhesions at the Calot's triangle.The cystic duct was ligated by silk.Results The operations were completed uneventfully in all the cases without converting to open surgery.No massive hemorrhage or biliary leakage occurred during the operation.The patients were followed up for 3 months to 3 years(mean,18 months),during which no one developed abdominal pain,fever,or jaundice.Conclusions Laparoscopic subtotal cholecystectomy without using titanium clips is a safe and simple method for patients with serious adhesions at the Calot's triangle.The rate of postoperative complications is low.