Objective:To investigate mRNA expression of cardiac related genes of NKX2.5,TBX5 and GATA4 in patients with tetralogy of fallot(TOF). Methods:A total of 10 TOF patients(TOF group)from 4 months to 8 years with the mean age of 3.5 years were recruited in our hospital from June to December 2005.The patients were diagnosed by typical clinical manifestation and cardiac color echocardiogram, the diagnosis was confirmed by cardiac surgery.6 non-congenital heart disease children were selected as Control group, and they were from 4 months to 9 years with the mean age of 3.8 years.The myocardial total RNA was extracted,the related cDNA was obtained by RT-PCR.The product cDNA was amplified with fluorescent quantitative PCR in order to compare the differences of NKX2.5,TBX5,GATA4 and GAPDH mRNA expression between TOF group and Control group. Results:NKX2.5 mRNA expression in TOF group was statistically decreased than that in Control group,while there were no statistical changes found in TBX5 and GATA4 mRNA expression between TOF group and Control group. Conclusion:The mRNA expression of NKX2.5,TBX5 and GATA4 were found in myocardium development.TOF was possibly related to decreased NKX2.5 mRNA expression.

The role of serotonergic system in vasovagal syncope (VVS) has been more concerned.Serotonin is a kind of neurotransmitter which is widely distributed in central nervous system.There are kinds of serotonergic receptors on postsynaptic membrane,among which 5-HT1A receptors participate in the mechanism of central blood pressure depression and promote the release of hormones such as prolactin and adrenocorticotrophic hormone.Acute administration of selective serotonin reuptake inhibitors (SSRIs) during head-up tilt test (HUT) blocks the reuptake of serotonin in the synapse space and thus increases stimulation of the serotonin receptors,which induces syncope episode.Long-term administration of SSRIs downregulates the postsynaptic serotonergic receptors,thus it weakens the reaction to the rapid change of serotonin concentration, which results in rapid depression of sympathic nerves and prevention of syncope episode.These demonstrate that central serotonergic system may participate in the pathogenesis of VVS and SSRIs are important for the diagnosis and treatment of VVS.

Arginine ; therapeutic use ; Burns ; immunology ; therapy ; Humans ; Nutrition Therapy ; methods

Objective　To investigate the interference of common methods in determination of creatinine. Methods　Standardized with Fuller′s earth, Jaffe′s method, enzymatic method (ultraviolet), enzymatic method (colour), enzymatic method (electrode) were compared and evaluated. Results　In eight interference materials, Jaffe′s method was positively affected by pyruvic acid (≥0.186 mmol/L), α-ketoglutaric acid(≥0.0615 g/L), ascorbic acid(≥0.82 mmol/L) and negatively affected by bilirubin(≥165.5 μmol/L). Enzymatic method (ultraviolet) was not interfered. Enzymatic method (color) was positively affected by creatine(≥0.21 mmol/L) and negatively interfered by dopamine(≥0.28 mmol/L). At last, enzymatic method (electrode) was positively interfered by creatine(≥0.62 mmol/L). Conclusion　Enzymatic method(ultraviolet) is the best in the above methods.

Objective To investigate the effects of long-term consumption of Fallopia multiflora on mouse hematopoietic system.Methods Forty 10-month old female C57BL/6J mice were equally divided into two groups at random, the control group fed with normal food , and the experimental group , given food with added Fallopia multiflora. After 10 month, the mice were sacrificed, and the peripheral blood, spleen, thymus and bone marrow cells were examined by flow cytometry.Results In the mice fed with Fallopia multiflora, the percentage of B cells in the spleen and CD 4 +cells in the thymus were increased , and CD8 + cells in the thymus and bone marrow hematopoietic stem cells were decreased , among the bone marrow cells , G0 cells were increased , but G1 and G2/S/M cells decreased .Conclusions Long-term proper consumption of Fallopia multiflora can delay the ageing of the hematopoietic system , and sustain its stability.

Interspinous process device is a kind of non-fusion implant for posterior spinal surgery.It is classified by static system and dynamic system according to its characteristics.With rapid development of internal fixator manufacture,materials for interspinous process device become increasing,such as bone allograft,titanium,poly(ether-ether-ketone) and lactoprene composite.A large number of experimental and clinical researches reveal the advantages of this technology in degenerative spinal stenosis,intervertebral original lower back pain,facet syndrome,protrusion of intervertebral disk,and lumbar instability.However,there are still many problems such as the influence on the lumbar posterior column,the mechanism to lumbar disc and the effect on the stability of spine etc.In addition,the clinical indications need to validate by long-term follow up.

Objective To study the effect of icariin on proliferation and phenotype of osteoblasts interfered with titanium particles. Methods Calvarial osteoblasts of newborn rats were cultured in vitro and cell identification was performed by alkaline phosphatase staining. Effect of titanium particles with different concentrations on osteoblasts proliferation was detected by CCK-8 method. Meanwhile , medium lethal concentration of titanium particles was screened out. Under this concentration , icariin with concentrations of 10-5, 10-6, 10-7, 10-8, 10-9 and 10-10 mol/L was added respectively for interference. The effect of icariin on proliferation of osteoblasts interfered with titanium particles was observed so as to obtain optimum drug concentration. Icariin with optimum concentraion was added into osteoblasts interfered with titanium particles. ALP activity of each group was detected by ELISA and calcified nodules were stained by alizarin S red to observe the effect of icariin on phenotype of osteoblasts. Results Titanium particles with different concentrations can inhibit proliferation of osteoblasts (P < 0.05), 0.5 mg/mL is its medium lethal concentration. Icariin can promote proliferation of osteoblasts interfered with particles (P < 0.05), the optimum drug concentration is 10-9 mol/L. With this concentration, icariin can significantly improve ALP activities of osteoblasts (P<0.05) and calcified nodules formation. Conclusion Icariin can promote proliferation and phenotypic expression of osteoblasts interfered with titanium particles. It may probably become an effective medicine for preventing aseptic joint prosthesis loosening.

Hypertension is a global problem,affecting both developed and developing nations. The mor-bidity of hypertension in children and adolescents has increased year by year in recent years. Hypertension in children can cause target organ damages(mainly renal,cardiovascular,central neurological,or optic fundal). In clinical practice,we need to find simple and easy methods for early recognition of target organ damages,thus se-rious consequences in adult hypertension may be prevented.

AIM:To study the relationship between the genetic polymorphism of CYP3A5 gene and the blood concentration/dose(C/D) ratio of cyclosporine combined with calcium channel blockers(CCBs) in renal transplant patients.METHODS:Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis was applied to determine the genotype of CYP3A5 in 69 renal transplant patients.The blood concentration/dose(C/D) ratio of cyclosporine was compared in patients coadministrated of CCBs with different genetic polymorphism of CYP3A5.RESULTS:The cases of genotype of CYP3A51/1,CYP3A51/3 and CYP3A53/3 were 8,23 and 38,respectively.The mutation rate of CYP3A53 in renal transplant patients was 71.74%.The CsA C/D ratio of patients of CYP3A51/1 and CYP3A51/3 were 1.73 and 2.43 times higher than that in patients of CYP3A53/3.The CsA C/D ratio of patients(CYP3A51/1,CYP3A51/3) combined with CCBs had been obviously elevated(19.90?7.66 vs 36.01?8.18,34.33?7.87 vs 46.16?9.63 ng?mL-1 per mg?kg-1;P

The reversed-phase high-performance chromatography was developed for determing the content of berberine, palmatine, emodin and chlorogenic acid in Xiaoerniuhuang Powder on ODS column. The powder samples extracted with methanol were determined separatly at their particular absorption points using external standard method.This method had advantages of perfect separatin, high sensitivity, good reproducibility and simple operation.