Hepatocellular carcinoma (HCC) is one of the most common malignant tumor with increasing incidence worldwid.Most of patients with HCC are diagnosed at a late stage.Threrfore,the prognosis of HCC patients is generally poor with a 5-year survival rate of 20% if withoutoperration. Screening strategies including α-fetoprotein (AFP) and ultrasound every 6 months in patients with liver cirrhosis,the major risk factor for HCC development, have been recommended to detect HCC at earlier stages amenable to effective treatment strateges.AFP, however,is a marker with poor sensitivity and specificity and the ultrasound is highly dependent on the operator's experience.Apart from AFP, lens culinaris agglutinin-reactive AFP and des-gamma carbexyprothrombin and several other biomarkers(e.g., glypican-3,human hepatocyte growth factor,and insulin-like growth factor) have been proposed as markers for HCC detection.In addition,with recently employed techniques,such as gene-expressing microarrays and proteomics,it is to be expected that new HCC-specific markers will become available in the near future.For all such proposed markers,however,the clinical usefulness has to be carefully evaluated and validated.

Antineoplastic Agents ; therapeutic use ; Benzamides ; Chemotherapy, Adjuvant ; Gastrointestinal Neoplasms ; drug therapy ; metabolism ; pathology ; surgery ; Gastrointestinal Stromal Tumors ; drug therapy ; metabolism ; pathology ; surgery ; Humans ; Imatinib Mesylate ; Indoles ; therapeutic use ; Mutation ; Neoplasm Staging ; Piperazines ; therapeutic use ; Proto-Oncogene Proteins c-kit ; metabolism ; Pyrimidines ; therapeutic use ; Pyrroles ; therapeutic use ; Receptor, Epidermal Growth Factor ; genetics ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism

The latest advances on target therapies of cancer were presented on the 44th Annual Meeting of American Society of Clinical Oncology(ASCO)in 2008.One of the most important results was the outstanding efficacy of Cetuximab in Metastatic Colorectal Cancer fIncao patients with k-ras"wild-type".Besides,detection of k-ras statuswouldmakeitpossibleforthetailoredtargettherapy of mCRC.Itwasestimatedthat k-ras gene status would become a predictive molecular marker for Cetuximab treatment in mCRC.

The opening rate, information update and menu of official WeChat in 11 Hangzhou municipal public hospitals were analyzed in order to understand their running, which showed that of the 11 public hospitals that opened their official WeChat,36. 36% opened their service account and 63. 64% opened their subscription ac-count. The information update frequency of WeChat varied and the online service function needed to be further deepened. Suggestions were put forward for the WeChat running in hospitals, such as normalizing its information push, deepening its online service, diversifying its communication between physicians and patients, and intensifying its popularization.

Objective:We try to examine the hypothesis that perceptual reversal is function of frontoparietal cortex. Methods:We employed block design in functional MRI, extracted event signal of endogenous reversal of Necker cube and compared with that of exogenous reversal. Results:Functional MRI revealed that spatial varieties were significant in bilateral superior and middle frontal lobe. Conclusion:Perceptual reversal may be the function of frontal cortex.

Objective To study the relationships between serum matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metal]oproteinase-2 (TIMP-2) with bone metabolic markers and bone mineral density (BMD) in postmenopausal Chinese women.Methods In this study,192 postmenopausal Chinese females aged 48-65 were selected.Serum MMP-2,TIMP-2,bone alkaline phosphatase (BAP),osteocalcin,bone cross-linked C-telopeptides of typeⅠcollagen (CTx) and urine bone cross-linked N-telopeptides of typeⅠcollagen (NTx) were measured by ELISA.And the ratio of MMP-2/TIMP-2 was calculated.BMD was measured by dual energy X- ray abserptiometry.According to the criteria of WHO,these women were assigned to 3 groups : the normal,the low BMD and the osteoporosis groups.Results (1) Serum MMP-2 level was significantly higher in postmenopausal women with osteoporosis (1388?121)?g/L than that in age-matched normal controls (1126?141)?g/L (P0.05).(2) Notable negative relations were found between MMP-2,ratio of MMP-2/TIMP-2 with BMD of lumbar spine and Ward's triangle,serum BAP and osteocalcin (all P0.05).(3) In osteoporosis women,notable negative correlations between MMP-2,ratio of MMP-2/TIMP-2 with BMD of lumbar spine,femoral neck and Ward's triangle,serum BAP and osteocalcin were found (all P

Objective To explore the relation between single umbilical artery with other malformations and pregnancy outcome,and to provide evidence for pregnancy counseling and diagnosis.Methods The outcomes of 92 fetuses with single umbilical artery diagnosed and delivered in Beijing Obstetrics and Gynecology Hospital Affiliated to Capital Medical University from September 2007 to July 2009 were followed up and retrospectively analyzed with Chi-square test.Results Among 25 292 pregnant women,92 cases were finally diagnosed as single umbilical artery giving the incidence of 0.36％,among which 57.6％ (53/92) of newborns survived without malformations,15.2％(14/92) survived with malformations and 27.2％ (25/92) died during perinatal period.There were 36 fetuses (39.1％,36/92) with single umbilical artery complicated with other malformations,among which 29 had single malformation (80.6 ％ ),and seven ( 19.4 ％ ) had multiple malformations.The incidence of heart defects was the highest among the complicated malformations,and then followed by central nervous system,digestive system,motor system and urinary system.Thirty-three fetuses with single umbilical artery accepted karyotype analysis.Chromosome abnormality was diagnosed in three subjects (9.1％,3/33).Twenty-three fetus (25.0％,23/92) associated with small for gestational age,and among which 18 fetuses (78.3％,18/23) died with a higher mortality than that (4.0％,24/597) of small for gestational age patients without single umbilical artery (x2=181.71,P＜0.01).Conclusions Single umbilical artery is likely to be complicated with congenital anomalies.After single umbilical artery was diagnosed by routine ultrasound,fetal ultrasonography,echocardiography and amniocentesis for karyotype analysis is suggested.Fetal growth restriction is an important indicator of fetal adverse outcomes.

Differentiated cells can be induced into pluripotent stem cell by reprogramming with defined fators. The new method of stem cell technology marks a major breathrough. This paper reviews the establishment, identification and research progress about induction of pluripotent stem cell.

Objective To review the pathogenesis and research prospects in AA.[Methods] In this paper, we summarize the imbalance mechanism of Th1/Th2, and the relationship of fol icular helper T cells(Tfh),Thl7, Th9 ,Th22 with aplastic anemia. [Results]The imbalance of Th1/Th2 cells leads to bone marrow failure. Immunosuppressive therapy can inhibit Th1 cell, restore the balance. The pathogenesis of Tfh, Thl7, Th9 and Th22 is closely correlated with AA. [Conclusion] AA pathogenesis is complex, CD4+cellsubsets is related to the occurrence and development of AA. Detect the levels of immune cells in the serum of patients is beneficial for diagnosis and treatment of AA.

Objective To explore the efficacy of zolpidem in improvement of sleep quality of patients received methadone maintenance treatment (MMT).Methods 80 cases of MMT with insomnia were randomly divided into the study group (administered with zolpidem)and the control group (administered with placebo), 40 cases in each group.Pittsburgh sleep quality index (PSQI)and treatment emergent symptoms scale (TESS)were assessed before treatment and at the weekend of 4th,8th after treatment.Results The scores of PSQI in the study group were much lower than those in the control group (t =-2.517,P =0.014;t =-3.611,P =0.000),while the effective rate in the study group was significantly higher than that in the control group (χ2 =4.949,P =0.026). Conclusion Zolpidem could improve the sleep quality of patients with MMT.