In order to improve the optimum temperature of lipases, The Penicillum expansum lipase (PEL) gene was mutated by site-directed mutagenesis using overlap extension PCR technique. The recombinant plasmid pPIC3.5K-lip-E83V containing matant gene was expressed in Pichia pastoris GS115. The comparison experiments of the mutant PEL-E83V-GS with the wild-type PEL-GS showed that: the optimum temperature of the mutant (45℃) was higher by 5℃ than that of the wild type. The thermostability of the mutant was similar to the wild type. The enzymatic activity of the mutant was 188 U/mL at 37 ℃, which was 80% that of the wild type at the same conditions. The hydrophobic interaction may be enhanced in the surface region by the hydrophilic amino acid, Glu substituted with the hydrophobic amino acid, Val, and be responsible for the improvement of optimum temperature.

Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; Carcinoma, Hepatocellular ; therapy ; Chemoembolization, Therapeutic ; Combined Modality Therapy ; Cyclopropanes ; administration & dosage ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Fluorouracil ; administration & dosage ; Humans ; Infusions, Intra-Arterial ; Liver Neoplasms ; therapy ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Mitomycin ; administration & dosage ; Phytotherapy

AIM: To study the effect of estrogen on injury induced by ?-amyloid protein (A?) in primary cultures of rat cortical neurons. METHODS: The effect of 17?-E_2 on A?(25-35)-induced cell death in primary rat cortical neurons was observed by phase contrast light microscopy, Giemsa staining and determination of lactate dehydrohenase (LDH) release rate. RESULTS: A?(25-35) induced cell death in rat primary cortical neurons. Forty eight hours pretreatment with 17?-E_2 protected rat primary cortical neurons from A?(25-35)-induced injury. CONCLUSION: A? evokes toxicity in rat primary cortical neurons. Estrogen can protect the rat primary cortical neurons against injury induced by A? (25-35).

Amblyopia is a developmental abnormality that results from physiological alterations in the visual cortex and impairs form vision. Based on multimedia, visual perceptual learning was widely applied in amblyopia treatment. Perceptual learning provided an impor-tant new method for treating amblyopia both in children and adults, and it reflects alterations of neural responses in the visual pathway. The therapeutic efficacy of perceptual learning was related to age, amblyopic types and degrees, training time and programs closely.

Humans ; Medical Staff ; Occupational Exposure ; prevention & control ; Occupational Health

Objective:To forecast the indicators on maternal and child health of China in 2020.Methods: Based on Surveillance data of the indicators on the maternal and child health in China since the 1990s,forecasting models were found using auto-regressive method,and the indicators on maternal and child health in China in 2020 were forecasted using the models after they had been tested and valued.Results: Auto-regressive models on infant mortality rate(IMR),under-5 mortality rate(U5MR) and maternal mortality(MMR) were found.The models and their parameters passed statistical tests,and their mean absolute error was 5% or so and determination coefficients were all more than 90%.Conclusion: The IMR of China in 2020 was forecasted to be 6.35‰,the U5MR 7.37‰ and the MMR 22.21/100 000.

Glycoprotein N is encoded by glycoprotein N (gN) gene of herpesviruses. The amino acid composition and expression level of this protein vary among difference species of herpesviruses. According to present studies, gN protein is expressed in cytoplasm of host cells, mainly in endoplasmic reticulum. The gN forms a complex with glycoprotein M in host cells. The complex is involved in the processes of viral replication and inter-cellular infection. Moreover, this protein plays a role in immune evasion from host immune system. The study will provide a theoretical basis for further study of herpesvirus gN gene and its encoded protein.
Animals ; Herpesviridae ; genetics ; metabolism ; Herpesviridae Infections ; virology ; Humans ; Viral Envelope Proteins ; genetics ; metabolism

Anxiety Disorders ; etiology ; therapy ; Breast Neoplasms ; complications ; therapy ; Humans ; Medicine, Chinese Traditional ; Qi

Objective To explore different techniques for treat me nt of complicated distal femoral fractures in order to obtain the best curative effects. Methods A retrospective study was done on the cli nical data of 62 patients (64 sides) with complicated distal femoral fractures a dmitted to our hospital from January 1999 to June 2002. According to AO classifi cation: 20 cases (21 sides) belonged to type A and 42 (43 sides) to type C. The supporting steel of AO femoral condyles were applied in 18 cases (19 sides), loc ked double-plating (steel plate and polyethylene plate) in 20 (21 sides) and th e retrograde intramedullary nail in 24. All cases received scientific and ration al rehabilitative treatment post-operatively. Results The 4-38 months follow up showed that 62 cases go t fracture healing with average healing period of 4.2 months (from 10 weeks to 1 6 months). According to criteria of Kolmert and Wulff, 31 sides were excellent, 28 good but 5 poor with an excellence rate of 75%. Conclusions A majority of treatment results of complicate d distal femoral fractures are satisfactory through rational and reliable intern al fixation. The supporting steel plate is suitable for all distal femoral fract ures; and the retrograde intramedullary nail is the treatment of choice for type A and type C1 fractures. As for types C2 and C3 fractures, the locked double-p lating combined with polyethylene is more effective than the supporting steel pl ate or the retrograde intramedullary nail for it has advantages of reasonable de sign, convenient operation and firm fixation.

0.05).But the detection rates of them were all higher than that of AFP alone(P