Objective To investigate the possible mechanisms of ox-LDL on vascular smooth muscle cells (VSMC) with and without exogenous zinc finger protein A20 gene. Methods VSMC were trans-fected with plamid containing A20 gene and incubated with ox-LDL. TLR4 and LOX-1 mRNA expression were measured by RT-PCR. Results After VSMC incubated with ox-LDL, the expression of LOX-1 and TLR-4 mRNA reached a higher level. However, when VSMC were transfected with plasmid containing A20 gene, the NF-kB nuclear translocation and the ability of migration reduced to normal level. Conclusions ox-LDL can significantly activate NF-kB signaling system and enhance migration in VSMC. The activation may induce the inflammatory response in arterial wall, the migration of VSMC to the intima, and triggering the process of atherosclerosis. Transfecting plasmid containing A20 gene significantly inhibited the activation of NF-kB and the ability of migration of VSMC. A20 effects might be through inhibiting NF-kB signaling. Meanwhile, A20 blocked the development of atherosclerosis.

Hyperthyroidism with periodic paralysis is common in clinic,the curative effect of peroral drugs on it is not satisfactory. In order to evaluate the treatment of 131 I for periodic paralysis in patients with hyperthyroidism, fouty two patients with hyperthyroidism complicated with periodic paralysis were put on p.o. 5.0 9.0 mCi 131 I Na treatment and the remission rate of periodic paralysis was observed.The total effective rate was over 95%. It can be concluded that 131 I could be used as a routine therapy for periodic paralysis in most patients with hyperthyroidism for its safety and efficiency.

Ultrasonic scalpels,including incision type and aspiration type,are introduced from aspects of the principles,key technologies and clinical application.Uitrasonic scalpel has the advantages of less bleeding,exact incision,,safety,shortened time of operation and less damage to surrounding tissue.Some technology problems of ultrasonic scalpel are also summarized.It is pointed out that ultrasonic scalpel is the tendency and direction of clinical surgery development,which has extensive application prospects.

OBJECTIVE: As a new method of altitude training, stimulated living high-training low training (HiLo), can solve the problems in traditional altitude training. In this paper, we review the effect of simulated living high-training low on the related indexes of red blood cells and the expression of erythropoietin and hypoxia inducible factor-1mRNA to improve the development and application of HiLo, and make it better service for our athletes. DATA SOURCES: A computer-based online search of PubMed was undertaken for the articles published between January 1972 and December 2005, among which partial articles were retrieved according to relevant references with the keywords of "simulated living high-training low training (HiLo), indices of red blood cell, effect; erythropoietin, hypoxia inducible factor-1; gene". Meanwhile, we searched http://cnki.hunnu.edu.cn for the relevant articles published between 1996 and 2006 with the same key words in Chinese. STUDY SELECTION: The articles that involved the effect on the indices of red blood cell and the expression of erythropoietin and hypoxia inducible factor-1 mRNA in the model of stimulated living high-training low training through the first trial sport were chosen after the first selection. Then, the full texts of these articles were looked up to identify whether they were the relevant studies. Inclusive criteria: ①randomised controlled experiments; ②the experiment including control and intervention groups. Exclusive criteria: ①review articles; ②repetitive studies. DATA EXTRACTION: Totally 90 relevant articles were collected, of which 38 were accordant with the inclusive criteria and the 52 repetitive studies or review articles were excluded. DATA SYNTHESIS: ①Levine discovered that in HiLo the maximal O2 consumption (VO2max) in the training group was increased 5% in the mean and red cell 9% and the ability of exercise was largely improved, while the results of the control group had no significant changes. ②HiLo training can induce the production of hypoxia inducible factor-1, which regulates erythropoietin, and its production. The increase in erythropoietin triggers an increase in red cell mass and Hb concentration. This hematological acclimatization response facilitates the restoration of blood O2 content and improves tissue oxygenation. Continuous residence at moderate heights (over 2 000-2 500 m) could improve the oxygen transport capacity, augment VO2 max and enhance exercise performance by increasing the content of erythropoietin and hemoglobin concentration. ③HiLo can induce the expression of hypoxia inducible factor-1, but the problems that how it regulates the expression of erythropoietin and transport the signal, and HiLo can alert the portion of blood, which affects the ability of exercise still need further study. Then we will more clearly learn the mechanism of the effect of HiLo on the ability of athletes, and help to enhance their performance. CONCLUSION: HiLo can increase the expression of erythropoietin and hypoxia inducible factor-1mRNA and the generation of red blood cells, which leads to an increase in hemoglobincon concentration and hematocrit, and effectively improves the ability of athletes.

Humans ; Methyl Ethers ; toxicity ; Occupational Exposure

Objective To observe the CT features of mycoplasma pneumoniae pneumonia. Methods CT features of 40 adult patients with serologically proven mycoplasma pneumoniae pneumonia were retrospectively reviewed. Results The most common CT findings of mycoplasma pneumoniae pneumonia were ground-glass opacity (34/40, 85.00%) and consolidation (28/40, 70.00%). The other findings included nodules (18/40, 45.00%), thickening of the bronchovascular bundles (6/40, 15.00%), interlobular septal thickening (6/40, 15.00%), mediastinal lymphadenopathy (6/40, 15.00%) and pleural effusion (4/40, 10.00%). Most of the ground-glass opacity and consolidation showed tendency of lobular distribution and involving the lower lobes. In some patients, the absorption of the lesions was slower than the relief of symptoms. Conclusion CT findings of mycoplasma pneumoniae pneumonia have some features, and can provide help for the diagnosis of mycoplasma pneumoniae pneumonia.

GC retention index of volatilc component of Cyperus rotundus was studied by capillary Gas Chromatography, and the standard Gas Chromatographic diagram and GC retention in dex spectrum, which provide a new identification method for traditional Chinese medicine was established. This method may be used to difierentiate the genuine C. rotundus from other false or confusion products. The average CV (% ) of RI from 37 sets of C. rotundus from three differcnt producing areas were 0~0.24 The method posscsses well accuracy, comparability and good reproducibility

0.05).Conclusions:Facial nerve excited threshold detection was a simple and effective method of conjecturing TCM syndrome and severity and prognosis of facial paralysis patients.

Objective To investigate the effect of transcranial direct current stimulation combined with motor relearning program on the recovery of upper limb motor function after stroke.Methods From September 2015 to June 2016,40 patients with first-ever stroke received rehabilitation therapy at the Department of Rehabilitation Medicine,Xuanwu Hospital,Capital Medical University were enrolled prospectively.Their course of disease was 1 to 6 months.They were divided into either a combined treatment group or a control group according to the odd and even numbers in hospital (n =20 in each group).The patients of both groups received traditional rehabilitation training and motor relearning program for upper lindb recovery,2 times a day,once for 30 min,5 days a week for 6 weeks.The combined treatment group was also given transcranial direct current stimulation,2 times a day,once for 20 min,5 days a week,while the control group was only treated with sham stimulation treatment.The patients were assessed before treatment and at 6 weeks after treatment.Fugl-Meyer Assessment (FMA) scores and Action Research Arm Test (ARAT) were used to assess upper limb motor function.The modified Barthel index (MBI) was used to evaluate the activities of daily living.Results There was no significant difference in the observation indices before treatment between the 2 groups (P ＞ 0.05).Six weeks after treatment,the differences of the upper limb FMA score and ARAT score before treatment were superior to the control group,they were 13 ± 3,10 ± 3 and 10 ± 2,8 ± 2,respectively (t =3.503 and 3.244 respectively,all P ＜ 0.01).There was no significant difference in the MBI score between the 2 groups (P ＞ 0.05).Conclusion Transcranial direct current stimulation combined with motor relearning program may effectively improve the upper limb motor function in patients with stroke and promote the improvement of the activities of daily living.

OBJECTIVE:To establish the quality standard for Maikang mixture. METHODS:TLC was used for the qualitative identification of Paeonia lactiflora,Angelica sinensis,Ligusticum chuanxiong,Astragalus membranaceus,Schisandra chinensis and Ophiopogon japonicus. HPLC was used for the content determination of schisandrin:the column was Kromasil C18 with mobile phase of methanol-water (60∶40,V/V) at a flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 40 ℃,and the infection volume was 20 μl. RESULTS:P. lactiflora,A. sinensis,L. chuanxiong,A. membranaceus,S. chinensis and O. japonicus showed clear spots and well separated. The linear range of schisandrin was 1-50 μg/ml (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 97.01%-98.58%(RSD=0.56%,n=6). CONCLU-SIONS:The established standard can be used for the quality control of Maikang mixture.