OBJECTIVE:To analyze the structure of tamsulosin hydrochloride by spectral data.METHODS:The spectra of 1H NMR,DEPT,13C NMR,1H-1HCOSY,HMQC、HMBC,MS,FT-IR and UV of tamsulosin hydrochloride were determined and interpreted.RESULTS:All the 1H NMR and 13C NMR chemical shifts were assigned rationally.The peaks of functional group vibrations(FT-IR),the main fragment peaks(MS)and UV characteristic absorption were all explained reasonably.CONCLUSION:The above analytical data of all kinds of spectra coincided with the structure of tamsulosin hydrochloride.

OBJECTIVE:To study the toxic effect of FKA on the domestic rabbits and guinea pigs.METHODS:KFA tablets were given to the domestic rabbits through vagina as single-dose and multi-doses respectively,on which acute toxic and irritating tests were conducted;the general condition of which were observed to see if there was any abnormality or the occurrence of erythema or edema in the vagina tissues;whereas the guinea pig was given dermal allergic reaction test.RESUL_ TS:There was no significant difference in the general state of the domestic rabbit after treatment and its stimulus-responses score was0point;there was no dermal allergic reaction in the guinea pig after treatment with FKA.CONCLUSION:FKA is safe and innoxious for the domestic rabbits and guinea pigs.

Humans ; Psychological Tests ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; psychology

Objective To investigate the expression and significance of hypoxia-inducible factor 1 alpha (HIF- 1α), vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) in tissues of condy- loma acuminatum (CA) in pregnant women. Methods Tissue specimens were obtained from the lesions of 30 pregnant women with CA and 30 non-pregnant women with CA, and from the vulva of 15 normal preg- nant women. By immunohistochemical staining, the expressions of HIF -1α, VEGF and Ang-2 were detected in these specimens. Results The expression rates of HIF -1α,VEGF and Ang-2 were 86.67%, 93.33% and 83.33% in pregnant women with CA, respectively, 63.33%, 66.67% and 53.33% in non-pregnant women with CA, respectively, and 0, 6.67% and 0 in normal women, respectively. Enhanced expressions of HIF -1α,VEGF and Ang-2 were observed in pregnant women with CA compared with the latter two groups (P < 0.05). In pregnant women with CA, a significant correlation was noted between the expression of HIF -1α and VEGF (r = 0.412, P < 0.01) and between the expression of Ang-2 and VEGF (r = 0.460, P < 0.01). Further more, the expression of HIF -1α, VEGF and Ang-2 positively correlated with each other in non-preg- nant women with CA. Conclusion In tissues of CA in pregnant women, HIF-1α,Ang-2 and VEGF are over expressed, which may be related to angiogenesis.

Objective To detect the expression of DNA repair enzyme O6-methylguanine DNA methyl-transferase(MGMT),cell proliferation-related nuclear protein(Ki-67)and P53 protein in gliomas,and inves-tigate the relationship and clinical significance among them and gloma grade. Methods 61 cases of brain glio-ma specimes and 16 cases of internal decompression of brain trauma were used to detect the expression of MGMT,Ki-67 and P53 by using immunohistochemical SP method. Results MGMT protein expression (19. 67%∶0,χ2 =3. 729,P=0. 062),Ki-67 protein expression(39. 34% ∶0,χ2 =5. 722,P=0. 016)and P53 protein expression(27. 87% ∶0,χ2 =9. 146,P=0. 002)showed significant differences in gliomas com-pared to normal brain tissues,the expression of Ki-67 was significantly higher in high-grade gliomas(Ⅲ-Ⅳ) than that in low-grade gliomas(Ⅰ-Ⅱ)(14 ∶10,χ2 =11. 718,P =0. 001). No significant difference was found between the MGMT and P53. Conclusion MGMT protein can be used as a biomarker in gliomas detec-tion;Ki-67 has a positive correlation with tumor grade,and can be used as a reference indicator of pathological grade;P53 protein expression may be used as a potential target for the treatment of gliomas.

Objective To evaluate the effect of the miracle questions in solution?focused brief therapy on memory tend and emotional stage in a non?clinical situation in college students. Methods A quasi?experimental design was adopted,and two kinds of materials for self?coaching( solution?focused,problem?focused) were devel?oped according to the previous researches. Seventy?two subjects were randomly divided into two groups,receiving the miracle questions in solution?focused brief therapy( The miracle question group,thirty?six subjects) or problem?focused coaching intervention(problem?focused group,thirty?six subjects).Exception memories number and positive and negative emotion level were evaluated between the two groups. Results The subjects receiving the miracle question group write more exception memories (3.67±2.07) than problem?focused group (1.61±1.18) ( t=5.178, P<0.01) . There was no significant difference in the positive and negative emotion level between the two groups(P>0.05) . Conclusion The miracle questions in solution?focused brief therapy can trigger the subjects to exceptional memories .This suggests that in practical application,questions order has impact on the effect of consultation. Be?fore asking exception question,miracle questions should be asked in the use of solution?focused intervention.

Objective:To study the curative efficacy of functional training focusing on muscle group around shoulder for subacromial impingement syndrome.Method:Forty five patients with subacromial impingement syndrome (SIS) were randomly grouped into three groups (fifteen patients for each group).Patients in Group Ⅰ were trained only on muscles around scapula.Patients in Group Ⅱ were treated on rotator cuff muscles.While patients in Group Ⅲ were treated on both.The training goes every 2 days and last for 4 weeks.The indexes for evaluating curative efficacy included:the Neer-test,the Hawkins-test and the Peen Shoulder Score.These indexes were measured immediately and three months after the four week treatment.Result:After treatment of the functional training focusing on muscle group around shoulder,Neer-tests and Hawkins-tests of all of patients were changed from positive to negative,the degree of pain compared with pain before the treatment declined apparently(P＜0.01),at the same time,the degree of satisfaction and the function went up remarkablely(P＜0.01).Compared with group Ⅰ and group Ⅱ,it dropped the most notably on the degree of pain in the group Ⅲ(P＜0.01),however,the degree of satisfaction and the function rose most apparently (P＜0.01) Conclusion:The curative efficacy of functional training focusing on muscle group around shoulder for sub-acromial impingement syndrome was effective apparently.But the efficacy of treated focusing on both muscles around scapula and rotator cuff was the most remarkably.

Object To study the chemical constituents from fruiting bodies of Ganoderma lucidum (Leyss ex Fr.) Karst Methods Individual constituent was isolated and repeatedly purified on silica gel column and structurally elucidated by the NMR spectra and X ray diffraction analysis Results Four compounds were obtained from the alcohol extract and identified as 3? oxo formyl 7?, 12? dihydroxy 4, 4, 14? trimethyl 5? chol 11, 15 dioxo 8 en (E) 24 oic acid (Ⅵ); 3? oxo formyl 7?, 12? dihydroxy 5? lanost 11, 15, 23 trioxo 8 en (E) 26 oic acid (Ⅶ); ergosterol (Ⅷ) and ergosta 7, 22 dien 3? ol (Ⅸ), respectively Conclusion Compound Ⅵ and Ⅶ are new triterpenes

Objective:To provide reference for clinical pharmacist in the treatment of severe bone marrow suppression complicated with infection induced by chemotherapeutic drugs .Methods:The pharmaceutical care was performed by clinical pharmacist for a pa-tient with severe bone marrow suppression complicated with pulmonary infection caused by chemotherapy .The suggestions on the drug use in the evaluation of chemotherapy regimen and the treatment of bone marrow suppression and infection were provided .Results:The bone marrow inhibition was relieved , the pulmonary infection was improved , and no other severe adverse reactions were shown .Con-clusion:Clinical pharmacist can improve effectiveness and safety in the treatment of patients with severe bone marrow suppression by providing individualized drug treatment .

The‘prevention and early treatment of diseases’conception as‘Huangdi Neijing’suggested,is the highest academic realm among the Chinese medical theory,it flows through the entire process of Chinese medical theory and diseases prevention.Four aspects of the prevention conception manifested in the process of Chinese medical prevention of diseases are: Taking preventive measures before confirmed diagnosis(health cultivation),impeding diffusion after confirmed diagnosis, deterring diffusion from deterioration,and restraining deterioration from being malignant.The malignant causal chain conversion of the coexistence of asthenia and sthenia is considered as the inducing links of critical illness’occurrence,evolution and transformation.Therefore,to intercept the malignant causal chain conversion comes to the key technique of Chinese medical remedy for critical illness.The‘prevention and early treatment of diseases’conception must impenetrate the versal process of treating critical illness.The overall clue and method of Chinese medical remedy for critical illness is to identify and grasp the vital contradiction and each respect of the malignant causal chain conversion which concurs with the evolution of the critical illness,simultaneously utilize the method which combined the prevention in etiology and pathogenesis with the remedy in etiology and pathogenesis,then actuate the pathologically changed organism to extricate from critical conditions at the first opportunity, finalizing with convalescence and recuperation thereafter.