OBJECTIVE:To study the toxic effect of FKA on the domestic rabbits and guinea pigs.METHODS:KFA tablets were given to the domestic rabbits through vagina as single-dose and multi-doses respectively,on which acute toxic and irritating tests were conducted;the general condition of which were observed to see if there was any abnormality or the occurrence of erythema or edema in the vagina tissues;whereas the guinea pig was given dermal allergic reaction test.RESUL_ TS:There was no significant difference in the general state of the domestic rabbit after treatment and its stimulus-responses score was0point;there was no dermal allergic reaction in the guinea pig after treatment with FKA.CONCLUSION:FKA is safe and innoxious for the domestic rabbits and guinea pigs.

OBJECTIVE:To analyze the structure of tamsulosin hydrochloride by spectral data.METHODS:The spectra of 1H NMR,DEPT,13C NMR,1H-1HCOSY,HMQC、HMBC,MS,FT-IR and UV of tamsulosin hydrochloride were determined and interpreted.RESULTS:All the 1H NMR and 13C NMR chemical shifts were assigned rationally.The peaks of functional group vibrations(FT-IR),the main fragment peaks(MS)and UV characteristic absorption were all explained reasonably.CONCLUSION:The above analytical data of all kinds of spectra coincided with the structure of tamsulosin hydrochloride.

Humans ; Psychological Tests ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; psychology

Objective:To provide reference for clinical pharmacist in the treatment of severe bone marrow suppression complicated with infection induced by chemotherapeutic drugs .Methods:The pharmaceutical care was performed by clinical pharmacist for a pa-tient with severe bone marrow suppression complicated with pulmonary infection caused by chemotherapy .The suggestions on the drug use in the evaluation of chemotherapy regimen and the treatment of bone marrow suppression and infection were provided .Results:The bone marrow inhibition was relieved , the pulmonary infection was improved , and no other severe adverse reactions were shown .Con-clusion:Clinical pharmacist can improve effectiveness and safety in the treatment of patients with severe bone marrow suppression by providing individualized drug treatment .

Objective To detect the expression of DNA repair enzyme O6-methylguanine DNA methyl-transferase(MGMT),cell proliferation-related nuclear protein(Ki-67)and P53 protein in gliomas,and inves-tigate the relationship and clinical significance among them and gloma grade. Methods 61 cases of brain glio-ma specimes and 16 cases of internal decompression of brain trauma were used to detect the expression of MGMT,Ki-67 and P53 by using immunohistochemical SP method. Results MGMT protein expression (19. 67%∶0,χ2 =3. 729,P=0. 062),Ki-67 protein expression(39. 34% ∶0,χ2 =5. 722,P=0. 016)and P53 protein expression(27. 87% ∶0,χ2 =9. 146,P=0. 002)showed significant differences in gliomas com-pared to normal brain tissues,the expression of Ki-67 was significantly higher in high-grade gliomas(Ⅲ-Ⅳ) than that in low-grade gliomas(Ⅰ-Ⅱ)(14 ∶10,χ2 =11. 718,P =0. 001). No significant difference was found between the MGMT and P53. Conclusion MGMT protein can be used as a biomarker in gliomas detec-tion;Ki-67 has a positive correlation with tumor grade,and can be used as a reference indicator of pathological grade;P53 protein expression may be used as a potential target for the treatment of gliomas.

Objective To evaluate the effect of the miracle questions in solution?focused brief therapy on memory tend and emotional stage in a non?clinical situation in college students. Methods A quasi?experimental design was adopted,and two kinds of materials for self?coaching( solution?focused,problem?focused) were devel?oped according to the previous researches. Seventy?two subjects were randomly divided into two groups,receiving the miracle questions in solution?focused brief therapy( The miracle question group,thirty?six subjects) or problem?focused coaching intervention(problem?focused group,thirty?six subjects).Exception memories number and positive and negative emotion level were evaluated between the two groups. Results The subjects receiving the miracle question group write more exception memories (3.67±2.07) than problem?focused group (1.61±1.18) ( t=5.178, P<0.01) . There was no significant difference in the positive and negative emotion level between the two groups(P>0.05) . Conclusion The miracle questions in solution?focused brief therapy can trigger the subjects to exceptional memories .This suggests that in practical application,questions order has impact on the effect of consultation. Be?fore asking exception question,miracle questions should be asked in the use of solution?focused intervention.

The‘prevention and early treatment of diseases’conception as‘Huangdi Neijing’suggested,is the highest academic realm among the Chinese medical theory,it flows through the entire process of Chinese medical theory and diseases prevention.Four aspects of the prevention conception manifested in the process of Chinese medical prevention of diseases are: Taking preventive measures before confirmed diagnosis(health cultivation),impeding diffusion after confirmed diagnosis, deterring diffusion from deterioration,and restraining deterioration from being malignant.The malignant causal chain conversion of the coexistence of asthenia and sthenia is considered as the inducing links of critical illness’occurrence,evolution and transformation.Therefore,to intercept the malignant causal chain conversion comes to the key technique of Chinese medical remedy for critical illness.The‘prevention and early treatment of diseases’conception must impenetrate the versal process of treating critical illness.The overall clue and method of Chinese medical remedy for critical illness is to identify and grasp the vital contradiction and each respect of the malignant causal chain conversion which concurs with the evolution of the critical illness,simultaneously utilize the method which combined the prevention in etiology and pathogenesis with the remedy in etiology and pathogenesis,then actuate the pathologically changed organism to extricate from critical conditions at the first opportunity, finalizing with convalescence and recuperation thereafter.

Low power laser has attached more attention of researchers at home and abroad in prevention and treatment of sports injury and sports fatigue. Based on literature and research achievements,this paper reviews and analyzes the research actuality and advancement of low power laser therapy in the fields of sports medicine,and discusses the trend of its research and application.

Objective To investigate the expression and significance of hypoxia-inducible factor 1 alpha (HIF- 1α), vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) in tissues of condy- loma acuminatum (CA) in pregnant women. Methods Tissue specimens were obtained from the lesions of 30 pregnant women with CA and 30 non-pregnant women with CA, and from the vulva of 15 normal preg- nant women. By immunohistochemical staining, the expressions of HIF -1α, VEGF and Ang-2 were detected in these specimens. Results The expression rates of HIF -1α,VEGF and Ang-2 were 86.67%, 93.33% and 83.33% in pregnant women with CA, respectively, 63.33%, 66.67% and 53.33% in non-pregnant women with CA, respectively, and 0, 6.67% and 0 in normal women, respectively. Enhanced expressions of HIF -1α,VEGF and Ang-2 were observed in pregnant women with CA compared with the latter two groups (P < 0.05). In pregnant women with CA, a significant correlation was noted between the expression of HIF -1α and VEGF (r = 0.412, P < 0.01) and between the expression of Ang-2 and VEGF (r = 0.460, P < 0.01). Further more, the expression of HIF -1α, VEGF and Ang-2 positively correlated with each other in non-preg- nant women with CA. Conclusion In tissues of CA in pregnant women, HIF-1α,Ang-2 and VEGF are over expressed, which may be related to angiogenesis.

AIM:To investigate the clinical effects of traditional Chinese medicine, Si Ling San, on acute central serous chorioretinopathy (CSC).METHODS:We retrospectively analyzed the clinical data of 48 patients (48 eyes) with CSC, among whom 24 were given oral Si Ling San (as treatment group) and the other 24 did not receive any treatment (control group).The baseline and 1 or 3mo follow-up data for the best corrected visual acuity (BCVA), central macular thickness (CMT) and maximum height of subretinal fluid (SRF) were collected and then compared between the two groups.RESULTS:BCVA of the treatment group was not statistically different from the baseline level at 1mo (P>0.05), but it was significantly improved at 3mo (P<0.05).BCVA was better at 3mo than that at 1mo(P<0.05).In the control group, however, no significant improvement of BCVA was found at 1 or 3mo compared with baseline (both P>0.05).The BCVA of the two groups was not significantly different at baseline and 1 and 3mo follow-up (all P>0.05).In the treatment group CMT and SRF at 1 and 3mo were significantly lower than baseline (both P<0.05).CMT and SRF at 3mo were significantly lower than that at 1 month (both P<0.05).In the control group, CMT and SRF at 1mo were not different from baseline level (both P>0.05), while they were significantly decreased at 3mo follow-up (both P<0.05).CMT and SRF at 3mo were significantly lower than that at 1mo (both P<0.05).In addition, CMT and SRF of the treatment group at both 1 and 3mo were statistically lower than that of the control group (all P<0.05).CONCLUSION:This study preliminary suggest that oral Si Ling San has the potential to treat acute CSC.But further trials are warranted to study its role in improving the visual function, shortening the duration and reducing the recurrence of CSC.