Approxmately 97% of all transcriptional output of the human being is noncoding RNA(ncRNA),which has been found to play roles in a great variety of processes,and is becoming more andmore attractive.This review is a general intruduction to the concept,classification and functions ofncRNA.

The expression and function of NIS are the prerequisites of radioactive iodine ( RAI ) treatment for DTC, which in turn determine the iodine uptake and outcome in DTC patients. Studies for the factors that might influence the function of NIS for the development of redifferentiation therapy should be conducted in conjunction with the individualized course of treatment in DTC patients having poor iodine up?take in their thyroid tumors. This review summarizes the factors that influence the function and expression of NIS in these patients.

Objective To discuss the influence of hydrotherapy on jaundice of newborn. Methods We divided 400 newborn into the hydrotherapy group and the control group with 200 cases in each group randomly. The newborn in the hydrotherapy group received hydrotherapy from 24 hours to 30 days after born,one time per day and 15 minutes per time.The control group was given pure shower, the time of first defecation ,the time of meconinm turning yellow, jaundice index and incidence of hyperbilirubinemia were compared at the 1st,5th,10th and 30th days after born. Results The time of first defecation and the time of meconium turning yellow were advancer in the observation group than those in the control group(P<0.01 ).The jaundice index at the 5th,10th andf 30th days after born and the incidence of hyperbilirubinemia also decreased in the observation group compared with those of the control group (P<0.01 ). Conclusion Hydrotherapy could decrease the jaundice of newborn and prevent the incidence of kernicterus.

Cochlear Implantation ; adverse effects ; methods ; rehabilitation ; Hearing Tests ; Humans

AIM To investigate antitumor effects of curcumin (Cur) combining with adriamycin(ADM) on human tumor cell lines in vitro . METHODS The antitumor effects of the drugs on tumor cell lines in vitro were determined with MTT method. The Jin's formula was used to analyse the effect of drug combination. RESULTS In simultaneous adiministration Cur 2 04 ?mol?L -1 ～16 29 ?mol?L -1 combining with ADM 0 70 ?mol?L -1 ～5 52 ?mol?L -1 produced a simple addition or potentiation effect. In sequential adiministration the first adiministration of ADM followed by Cur resulted in an antagonistic effect, while the change of the order of adiministration produced an simple addition effect. CONCLUSION Simultaneous adiministration of Cur and ADM produces synergistic effect but sequential adimistration of the drugs only produce a unidirectional synergistic effect.

Objective To investigate the etiologic factors of chronic prostatitis following sexually transmitted urethritis. Methods The pathogens have been detected in 86 patients with chronic prostatitis following sexually transmitted urethritis. Results 57 strains (66.3%) of bacteria were cultured in expressed prostatic secretions(EPS) from 86 patients,in which gonococcus was the majority of cultures,accounting for 38.4%. Chlamydia trachomatis (CT), ureaplasma urialyticum (UU) and gonococcus were also detected by polymerase chain reaction (PCR) in expressed prostatic secretions,25.6%(22/86), 22.0%(19/86),and 43.0%(37/86) showed positive results respectively. Conclusions Gonococcus,chlamydia trachomatis and ureaplasma urialyticum were the main pathogens of chronic prostatitis.The results indicate that pathogen examination plays an important role in treatment of chronic prostatitis following sexually transmitted urethritis.

Objective To evaluate the role of 3 D spiral computerized tomographic angiography (SCTA) in microsurgery of separating craniopagus twins Methods A case of craniopagus twins underwent spiral CTA Image reconstruction was made by shaded surface display Result SCTA demonstrated osseous fusion in twins skull Parts of bone and dura mater were found to be absent in the fused area Two parts of brain tissues were linked together, and some area were fused Twins' one third of posterior superior sagittal sinuses were fused and converged into a common sinus One side of their transverse sinuses were fused Conclusion SCTA can clearlly reveal the abnormalities of skull, cerebrum, intracranial blood vessels, especially the stereostruction between intracranial blood vessels and skull SCTA is useful for intraoperative bone design and decision of surgical route and has important value in formulating surgical plans

Protein protein binding is the basis of virus and host cell interactions. With the application of technology of studying of protein interactions, more knowledge of replication and pathogenesis of hepatitis C virus (HCV) could be acquired. Non structure protein NS5A is one of the important regulatory factors in virus replication , transcription and signal transduction, but there are controversy in effect on HCV pathogenesis and resistance to interferon ?(IFN?). In order to describe the relationship between NS5A and host proteins, we use yeast two hybrid system 3 to screen the gene encoding proteins that could interact with NS5A from human hepatocyte library. Thirty five clones were obtained including apo A1, apo A2, apo B100, haplotype mitochondrion complete genome, phosphatidic acid phosphatase type 2B, albumin similar to tumor endothelial marker 5 precursor, matrix metalloproteinase 14, and three of the Homo sapiens hypothetical proteins. The study paved a way for further studies on the pathogenesis of HCV NS5A

To clone the genes of hepatocyte protein interacting with hepatitis C virus NS3 protein, "bait" plasmids of hepatitis C virus NS3 were constructed. After verifying that hepatitis C virus NS3 protein could be steadily expressed in AH109 yeast strain, yeast two hybrid assay was berformed by mating AH109 with Y187 that pre transformed with liver cDNA library plasmids pACT2, and the diploidy yeast cells were plated on quadruple dropout (QDO) medium and assayed for X ? gal activity. Nineteen yeast colonies that could grow on QDO and had ? gal activity were obtained, then the library plasmids were extracted and sequenced. The gene sequences from the 19 positive colonies were aligned with the genes deposited in GenBank. It was found hepatitis C virus NS3 protein could interact with some proteins which have different functions.

The nonstructural protein 5A (NS5A) of the hepatitis C virus (HCV) has been shown to interact with a variety of cellular proteins and implicated in the regulation of cell growth, interferon resistance, and other cellular signaling pathways. Using the yeast-two hybrid method, we have isolated a clone that encodes a novel NS5A--associated binding protein: NS5ABP37. Reverse transcription polymerase chain reaction (RT-PCR) method was employed to amplify the full fragment,and the plasmid pGADT7-NS5ABP37 with the Saccharomyces cerevisiae vector pGADT7 was constructed. To prove the interaction, yeast cell Y187 transformed with pGADT7-NS5ABP37 was mated with yeast cell AH109 containing pGBKT7-NS5A to verify the interaction between the novel protein coded by the new gene NS5ABP37 and NS5A.