Objective:To investigate whether lanthanum(La 3+)can enter into ossification induced bone marrow stromal cells(BMSCs)cultured in vitro and its influences on the ultrastructure of BMSCs.Methods:Dog BMSCs were ossification induced and the cells of the third passage were cultuerd with the presence of lanthanum chloride(LaCl_3)at 5.564?102,5.564 and 5.564?10-2 ?g/ml respectively for 5 days,the same passage cells without LaCl_3 treatment were used as the controls.Transmission electron microscope(TEM),electron microprobe with wave spectrum(EMWS)and high resolution TEM with energy spectrum(HRTEM)were used to observe the change of BMSCs ultrastructure and the distribution of lanthanum ions in the cells.Results:Electron-dense granules in lysosome were observed in BMSCs treated with LaCl_3 but not in control BMSCs under TEM.Slight denaturation of some organelles was found in both LaCl_3 treated and control cells.EMWS examination showed that there was La element in BMSCs treated with 5.564?102 ?g/ml LaCl_3.La element was not found in all the treated cells under HRTEM.Conclusion:La 3+ at a certain concentration might enter into the ossification induced BMSCs cultured in vitro.

Objective:To determine the prevalence and compare the related factors of depressive symptoms among urban primary school students in Hefei and Shenzhen.Methods:Participants from Hefei and Shenzhen were asked to fill out the questionnaires including Children's Depression Inventory(CDI)and items of Social Support and demography.Results:The prevalence of depressive symptoms was significantly higher among the participants in Hefei(11.9%)than that in Shenzhen(8.2%)(?2 =10.37,P=0.001).The students with depressive symptoms had significant lower social support scores than those with no depressive symptoms in Hefei and Shenzheng cities(Parents support score was 3.6?1.6 vs 5.0?0.9,school support score was 2.1?0.9 vs 2.6?0.8,peer support score was 3.2?1.5 vs 4.4?1.0,total support score was 8.9?2.9 vs 12.0?1.8 in Hefei.Parents support score was 3.4?1.8 vs 4.9?0.7,school support score was 2.2?1.2 vs 2.9?0.8,peer support score was 3.4?1.5 vs 4.4?0.9,total support score was 9.0?2.7/12.3?1.6 in Shenzheng,all P

Adolescent ; Adult ; Aged ; Aspartate Aminotransferases ; metabolism ; Case-Control Studies ; Electrocardiography ; Endosulfan ; poisoning ; Female ; Humans ; Lactate Dehydrogenases ; metabolism ; Male ; Middle Aged ; Young Adult

Objective To investigate the HBV- positive pregnant women about the knowledge of the route of hepatitis B transmission, hepatitis B hazards, health care during pregnancy, announcements of child- care to explore targeted and effective health education model with self- designed question-naires. Methods Nurses in obstetrics department carried out this questionnaire investigation in the outpa-tients of HBV- positive pregnant women, after that the results underwent statistic analysis. Results The overall knowledge of HBV- positive pregnant women in announcements of child- care, health care during pregnancy and hepatitis B hazards was not very well, and the announcements of child- care was the lowest, followed by health care during pregnancy and hepatitis B hazards. The route of hepatitis B transmission was better, but still not satisfactory. The education should highlight these areas. Well- educated women were better than those who lack education. The age between 20 to 30 years old was better. Conclusions The HBV-positive pregnant women is lack of heath knowledge, so we should gave health intervention in a multi- level, multi- stage standardized and a variety of ways.

Objective To evaluate the strictness and self-consistency of the charge nurses in the nurses examination using venipunctnre trocar operation as research object.Methods FACETS,the polyhedral Rasch software was used in the examination.Results The strictness and self-consistency of charge nurses were evidently different.Conclusions The variable strictness and self-consistency of the charge nurses in the examination leads to unreliability of the results.Developing a scientific and reliable evaluation system is essential to improve the ability of the teaching nurses and the student nurses as well as the hospital nursing quality.

The viable but noncuturable (VBNC) state is a survival strategy when bacteria are exposed to en- vironment stress. The VBNC state is part of the life cycle of non-differentiating bacteria, and it has a far-reaching impact on traditional bacteriology. Cells in the VBNC state fail to grow on the routine bacterio- logical media, here its significance in human health and environment science are detailed. Cells entering the VBNC state exhibit dwarfing and a number of metabolic changes in respiration rates and macromolecular synthesis. This paper summarized the variations in DNA and protein comparing to the culturable cells. We also discussed the ability of cells to resuscitate from the VBNC state and return to an actively metabolizing and culturable form. Some new methods for monitoring the VBNC state were listed. Finally the future was suggested.

Abortion, Induced ; adverse effects ; Adult ; Female ; Humans ; Medicine, Chinese Traditional ; Pain, Postoperative ; therapy ; Pregnancy

Humans ; Occupational Exposure ; Risk Assessment ; Workplace

Objective To explore the clinic effect of intravascular stent angioplasty for extracranial internal carotid artery dissection(ICAD).Methods From July 2015 to December 2016, 25 cases of patients with extracranial ICAD were admitted into our hospital.Among them, there were 15 cases of head and neck pain,12 cases with transient ischemic or cerebral infarction symptoms (limb hemiplegia.etc.),6 cases of Horner syndrome,1 case with pulsatile tinnitus and syncope,2 cases with no obvious clinical symptoms which were found by the neck vascular ultrasound accidentally.All patients were treated with intravascular stent angioplasty.Calculated the degree of stenosis through digital subtraction angiography(DSA),measured the blood flow velocity with transcranial doppler(TCD),and observed the clinical manifestations.Reviewed those patients after six months.Results Totally 24 patients with ICAD fulfilled the intravascular stent angioplasty with no complications such as lining damage,bleeding and embolism.And one patient with ICAD abandoned intravascular stent angioplasty for the vascular is too circuity.DSA showed the average artery stenosis decreased from (76.70±10.57)% preoperatively to (11.07±4.18)% immediately after operation,and the difference was statistically significant(P<0.01).TCD measured the peak systolic velocity (PSV) of internal carotid artery stenosis segment,and the PSV changed from (189.55±54.27)cm/s averagely before operation to (88.41±17.06)cm/s on average one week after operation.The difference was statistically significant (P<0.01).Reviewed the head and neck CT angiography (CTA) after 6 months.There were 23 cases without new stenosis and 1 case had stent restenosis compared with CTA immediately after operation.Symptoms of 19 cases obviously remitted or disappeared,4 cases partially remitted,and recurrence occured in 1 patient.There was no appearance of infarction and other serious complications.Conclusion The intravascular stent angioplasty is relatively safe and effective for extracranial ICAD, which can remit the degree of stenosis,slow down the blood flow and relieve the clinical symptoms with low recent recurrence rate and adverse reaction.But it does not apply to patients with severe vascular circuity.