Objective:To explore the relationship between hypertension stage and blood pressure variability (BPV) in aged patients with hypertension ,and the relationship among target organ damage (TOD) ,age and BPV .Methods :A total of 180 aged inpatients with hypertension in our hospital received ambulatory blood pressure monitoring .Ac-cording to results of blood pressure monitoring ,patients were divided into hypertension stage 1 group (n=65) ,stage 2 group (n=61) and stage 3 group (n=54);according to their age ,they were divided into 60~70 years group (n=65) ,71~80 years group (n=68) and ≥81 years group (n=47) .BPV index was the measured standard deviation (SD) of blood pressure .Results:Along with hypertension stage rose (stage I~stage III) ,there was significant in-crease in BPV [24h mean BPV :(11.0 ± 2.5) mmHg vs .(12.2 ± 3.3) mmHg vs .(14.3 ± 3.6) mmHg , P<0.01 all] in aged patients with hypertension .For 24h systolic blood pressure (SBP) BPV /24h systolic blood pressure (DBP) BPV ,compared with those without TOD [(10.7 ± 2.3) / (8.0 ± 1.4) mmHg] ,there was significant rise in patients with heart damage [ (12.2 ± 2.4) / (9.8 ± 2.2) mmHg] ,brain damage [ (12.9 ± 3.1) / (9.6 ± 2.0) mm-Hg] and renal damage [ (15.7 ± 2.4) / (12.4 ± 1.5) mmHg]] , P< 0.05 or < 0.01. There was no significant difference in BPV among different age groups .Conclusion:Along with blood pressure rises ,blood pressure variabili-ty significantly increases in aged patients with hypertension ,and it's more significant in those with target organ dam-age .Relationship between blood pressure variability and age is not significant in these patients .

Immunoblotting(IB) technique has been frequently used to detect bullous pemphigoid (BP) antibodies in BP patient.It is important to extract the antigen proteins fully and intactly in the base membrane zone(BMZ) of normal human epidermis.The simple method of extracting antigens will make IB techniqne easy.Three different methods of extracting antigen proteins were compared.Using IB for detecting BP antibodies,it has been found that the results of epidermal extract preparations are basically similar.However,one of these methods (NO.1) is more convenient and may obtain more amounts of antigens.Another method(No.3) is identical to that of extracting antigens in dermis for de- tecting epidermolysis bullosa acquista (EBA) antibodies.Therefore,in using IB technique to distinguish BP from EBA,one can apply method No.3.

Adult ; Citric Acid ; adverse effects ; Dust ; Humans ; Male ; Middle Aged ; Occupational Exposure ; adverse effects ; Tooth Erosion ; chemically induced

The mutations and deletions of the MTS1/p16 gene are widespread in a variety of human tumor tissues.It has been shown that p16 plays an important role in the formation and development of cholangiocarcinoma as research on cholangiocarcinoma has progressed over the years, and it is thought that the abnormal expression of p16 in highly involved in cholangiocarcinoma-related diseases.All these findings indicate that p16 is closely related to cholangiocarcinoma.Early diagnosis of cholangiocarcinoma is still poor, so p16 may be a qualitative and quantitative tumor marker of high diagnostic value and may provide an opportunity for some new therapeutic methods such as targeted therapy.It can achieve the purpose of early diagnosis and early treatment to enhance the survival rate of patients.

Purpose:To evaluate multiple biomarkers of colorectal tumor and their potential usage in automated diagnosis of colorectal cancer.Methods:10 normal colonic mucosae, 30 colorectal adenomas and 53 colorectal carcinomas with complete follow-up data were examined for multiple biomarkers(DNA contents, AgNOR, PCNA and nuclear morphometry) with quantitative computed processed imaging analysis.Results:The values of most of the parameters analyzed increased in accordance with the normal mucosa-adenoma-adenocarcinoma sequence, indicating a marked tendency for progression of colorectal malignancy. Among those parameters, DNA index,positive rates, counts, density and aspect factor of AgNOR, positive rates and density of PCNA, shape factor, width and density of nucleus were demonstrated to be relatively valuable indices.Conclusions:The quantitative evaluation of some indices of colorectal tumor can provide reproducible data for differential diagnostic of this tumor.

Aim To establish the dementia mice models induced by intracerebroventricular infusion of ?-Amyloid peptide and related measuring index. Methods The mice models were made by intracerebroventricular infusion of long fragment of soluble?-Amyloid peptide with micropump, intracerebroventricular injection of long soluble?-Amyloid peptide fragment and short insoluble ?-Amyloid peptide fragment,and intracerebroventricular injection of ?-Amyloid peptide combining with transforming growth factor. Results Compared with normal control, the learning and memory ability was decreased in all above model mice. the ChAT activity in both hippocampus and cortex of model mice decreased, the hippocampal neuron were depleted and the content of apoptosis associated protein Bal-2?BAX and p53 were increased. Conclusions All above mice models could be choose as the dementia models which were simulating the pathological charactoristics of the Alzheimer's disease.

Catheter-Related Infections ; drug therapy ; prevention & control ; Catheters, Indwelling ; adverse effects ; Humans ; Intensive Care Units, Neonatal ; Thrombosis ; prevention & control

Objective The rapid latex scattering immune turbidimetric method detecting urine retinol binding protein, and evaluate methodology.MethodsIn accordance with the commission of American clinical laboratory standards (NCCLS) requirements, evaluation of the research methods of reagent sensitivity, precision, accuracy, stability, linear range, interference analysis, specific degrees.ResultsWith rapid latex scattering immune turbidity method to detect the urine retinol binding protein, for instance, the minimum detection limit of 0.0381 mg/L;Repeatability precision is 1.198%, intermediate precision is 5.541%, reproducibility precision is 6.662%;The linear range is within 0~10 mg/L;Recovery rate were 99.00% and 104.00% respectively;When TBIL<100mg/L and Hb<10mg/L, the detection of Tbil, Hb, RBP interference;With the urine RBP than kit in automatic analyzer compared the test results of analysis showed that r2=0.9720, correlation can be;50 cases of clinical specimens using two methods showed no statistically significant difference positive rate (χ2=0.948, P=0.948).ConclusionRapid latex scattering immune turbidimetric method were used to detect the urine retinol binding protein, has high precision, high accuracy, the advantages of high sensitivity, stability and anti-jamming is strong, good.

Cardiac involvement related to iron overload caused by long-term blood transfusion is a major cause of death for patients with thalassemia major. Patients' survival will be improved greatly if cardiomyopathy resulted from transfusional iron overload could be detected timely and effective iron chelation be initiated promptly. In the present article, various evaluation Methods currently used in clinical practice are reviewed, with particular focus on cardiac MRI for the monitoring of myocardial involvement related to iron overload.

CENP-Fisacell-cycledependentkinetochoreprotein,whoseexpressionandlocalization patterns are regulated in a cell cycle-dependent manner.CENP-F plays an important role in the development and progress of malignant tumor.The expression of CENP-F is up-regulated in many tumors.It serves as an important marker in cell proliferation.Studies have demonstrated that CENP-F is involved in tumor genesis, progress,invasiveness,relapse and prognosis.CENP-F might be a valuable prognostic marker and potential therapy target in cancer treatment.