In the perspective of new media , has carried on the review to the current situation of medical students′life consciousness , and puts forward the nationwide medical students life education of subjectivity , and open features , explore “four in one” mode of medical students′life education , namely:the use of new media inte-gration of curriculum resources , consolidate the first classroom; using new media to expand audience , strengthen education;using new media foil emotional rendering , rich campus culture;using new media development hot spec-ulative , vowed to cultural practice .

0.05).CONCLUSION:Chitosan gynecologic suppository is safe,effective and more receptive for the patients,and the resistance is not easily produced for treatment of candidal vaginitis and bacteroidal vagina disease.Its advantage is that the patients can be treated without complete laboratory examination.

With the intensification of population floating in China, there has been a growing demand for ecdemic medical care. Because basic medical insurance in China conduct localized management, the differentiation between reimbursement policy and level in different areas result in the difficulty of immediate settlement. The great quantity of floating population are faced with rather heavy personal burden when need medical care at different locations, which to some extent restrict in-time healthcare for floating population. On the basis of analyzing the features of endemic medical care for floating population, it described three mode of typical immediate settlement on ecdemic medical care, discussed the main problems in restricting the flowing population get immediate settlement at different locations and put forward suggestions on improving the immediate settlement at different locations.

In order to improve the optimum temperature of lipases, The Penicillum expansum lipase (PEL) gene was mutated by site-directed mutagenesis using overlap extension PCR technique. The recombinant plasmid pPIC3.5K-lip-E83V containing matant gene was expressed in Pichia pastoris GS115. The comparison experiments of the mutant PEL-E83V-GS with the wild-type PEL-GS showed that: the optimum temperature of the mutant (45℃) was higher by 5℃ than that of the wild type. The thermostability of the mutant was similar to the wild type. The enzymatic activity of the mutant was 188 U/mL at 37 ℃, which was 80% that of the wild type at the same conditions. The hydrophobic interaction may be enhanced in the surface region by the hydrophilic amino acid, Glu substituted with the hydrophobic amino acid, Val, and be responsible for the improvement of optimum temperature.

· Mesenchymal stem cells ( MSCs ) are a population of multipotent stem cells with various neurotrophins from bone marrow which are widely used in tissue, cell repair and alternative research.Recently, some researches have shown that MSCs could enhance the viability of neurons under a pathological circumstance by secreting some neurotrophins. So the neuroprotection of MSCs can provide a new method of the treatment on retinopathy that it possible to promote cells survial and functional recovery.Here we make a brief review on the secretory function of neurotrophin and neuroprotective effects of MSCs on retinal cells and its application in the treatment of retinal disease.

China ; epidemiology ; Diagnostic Techniques and Procedures ; Epidemiologic Studies ; Hematologic Diseases ; diagnosis ; epidemiology ; Humans ; Incidence ; Meta-Analysis as Topic ; Multicenter Studies as Topic ; Prevalence ; Randomized Controlled Trials as Topic

On one hand this paper used co-culture method of rat normal or injuried sciatic nerves with newborn rat superior cervical ganglia (SCG) to observe that the neurite outgrowth of SCG induced by the nerve, on the other hand we made normal or injuried sciatic nerve extracts in order to observe their neurite-promoting effects on PC12 cells to various dilutions of nerve extracts. The results show that normal and injuried sciatic nerves contain neurite-promoting factors (NPFs) for SCG sympathetic neurons. However, the NPF activity of injuried nerve is higher than that of normal nerve and has no significant differences from 1-41 days postlesion. We have also observed the neurite-promoting effects of normal or injuried sciatic nerve extracts on PC12 cells. There is a maximal effect with the injuried nerve extract prepared at 4 days post-lesion. The neurite-promoting activity of injuried (12 hours to 42 days post-lesion) nerve extracts remain constant. The rate of neurite elongation in PC12 cells relate to the dilution of nerve extracts. Experimental analysis suggests that the nerve NPFs may be not the single but two or more factors which may be produced and released by Schwann cells.

OBJECTIVE:To prepare Shuansaitong capsules and establish a method for its quality control.METHODS: Distillation method was applied to extract volatile oil from volatile oil-containing medical material and water decoction method was used to extract physic liquor from other medical material. Suitable quantity of excipients was added to prepare Shuansaitong capsules.Qualitative identifications of Ligusticum chuanxiong,Panax quinquefolium,Salvia miltiorrhiza,Angelica sisensis were performed by TLC,and the content of Gastrodin was determined by HPLC.RESULTS:The quality of Shuansaitong capsules was completely in conformity with the related requirements of China Pharmacopeia.The TLC spots were clear,well-separated,specific and free from interferonce of negative sample.The linear range of gastrodin was 0.1～0.5 ?g with a correlation coefficient of 0.999 5.The average recovery was 96.59% with RSD=0.47% (n=6).CONCLUSION:The preparation technology of Shuansaitong capsules is simple and the determination result was accurate,thus suitable for the preparation and quality control of Shuansaitong capsules.

Background Many researches have reported the high proliferation ability of pterygium tissue.However,the origin of cells in pterygium tissue and their role in pathogenesis and recurrence of pterygium are below understand up to now.Objective This study is to explore the role of bone marrow derived stem cells and progenitor cells in the pathogenesis of pterygium.Methods Informed consent was obtained from 12 patients with the pterygium stretching the edge of the pupil prior to this trail.Pterygium specimen was obtained from 8 patients with primary pterygium and 4 patients with recurrent pterygium during the operation.The normal conjunctival tissue was obtained from the location of 10 mm from the pterygium in the operation eye.The expression levels of marker of the primitive haematopoietic progenitors(AC133),marker of the haematopoietic progenitor cells and endothelium (CD_(34)),marker of haematopoietic and stromal progenitor cells (C KIT ),and differentiation antigen of bone marrow fibroblast cells and nonhaematopoietic progenitor cells 1 (STRO 1) were detected by using immunohistochemistry.Results Various progenitor cell markers originated from bone marrow,including CD_(34),AC133,C KIT,STRO 1,were positively expressed in pterygium specimen rather than normal conjunctival specimen mainly in the basal epithelium or stroma of pterygia tissue.Majority of the positive cells were seen in the head of pterygium.C KIT positive cells were observed mainly in the basal epithelium and stroma of pterygium.The morphology of positive cells varied upon different distribution.Fibroblast like and spindle shaped positive cells were seen in the stroma layer of pterygium,and round or ovoid shaped cells were in basal epithelial layer.The distribution and shape of positive cells for AC133 and CD_(34) were similar to ones of C KIT but numbers of cells were varied between them.C KIT,AC133 and CD_(34) positive cells were coexpressed in basal epithelium and vascular endothelium.Expression of STRO 1 was found in the stroma layer not in the basal epithelium of pterygium.Similar distribution,numbers and shape of positive cells were seen between recurrent and primary pterygium.Conclusion The cells with stem cells markers and progenitor cells markers originated from bone marrow may play a role in the pathogenesis of pterygium.