Laser-induced breakdown spectroscopy has become a very attractive technique in the field of chemical analysis.The technique utilizes a laser focused on a small spot to create a micro-plasma on the sample surface.In recent years, laser-induced breakdown spectroscopy (LIBS) is a frontier analysis technique in spectrometric analysis, with the advantages such as real-time, online and non-contact analysis.The research and development of various instruments based on this technology has attracted great attention of researchers.In the article, we reviewed the research progress of LIBS instrument from the components and field equipment in recent years, mainly including portable LIBS, handheld LIBS and remote LIBS.

BACKGROUND:Currently a big controversy erupts in the repair of abdominal wal defects with composite patch. OBJECTIVE:To compare the composite patch and biological patch in the repair of abdominal wal defects in dogs. METHODS:Ten dogs were selected to prepare abdominal wal defect models, and then randomized into experimental group subjected to composite patch repair and control group subjected to biological patch repair. Incision adhesion was assessed at postoperative 30, 60, 90 days; patches and defect tissues were taken and detected histologicaly at postoperative 14 days. RESULTS AND CONCLUSION:The incision adhesion in the experimental group was milder than that in the control group at different time post operation (P < 0.05). At 14 days post operation, the material structure was clear with no signs of absorption in the experimental group, and there were a large amount of infiltrated inflammatory cels around the patch material, such as neutrophils and lymphocytes, and non-phagocytic patch was found; in the control group, the material structure was fuzzy with the presence of inflammatory cel infiltration and slight signs of absorption. Moreover, inflammation and fibrosis were severer in the control group than the experimental group. These findings indicate that compared with the biological patch, the composite patch is better to effectively reduce inflammatory reactions.

Medicine, Chinese Traditional ; Reproductive Techniques, Assisted

Despite the immense achievement in the field of IVF in recent years, many patients still suffer from recurrent implantation failure. Therefore, much attention has been drawn to its etiology and treatment. Chromosomal abnormality, sperm DNA damage, zona hardening, and inappropriate culture conditions are important factors that lead to recurrent implantation failure. Results of studies suggest that preimplantation genetic screening does not improve the rate of implantation or live birth. Comparative genomic hybridization array and single nucleotide polymorphism array could offer a more comprehensive screening of chromosomes. Assisted hatching may help to solve the problem of zona hardening in some situations. Co-culture and blastocyst transfer could be conducive to the improvement of the rates of implantation and pregnancy. Cytoplasmic transfer may give a solution to ooplasmic composition anomalies.
Chromosome Aberrations ; Coculture Techniques ; Embryo Implantation ; Embryo Transfer ; Female ; Fertilization in Vitro ; Germ Cells ; Humans ; Male ; Pregnancy ; Preimplantation Diagnosis ; Treatment Failure

ObjectiveTo investigate the clinical characteristics and the way of discovery in early stage.Methods68 cases with asymptomatic pulmonary carcinoma living in Chongqing City who were discovered within health checkup were retrospectively analyzesed. ResultsThere were 62 males and 6 females,ages ranging from 28 to 78 years with an average of 52 years. In all patients,there were 56 cases (82.4%) with cigarette using history, 42 cases (61.8%) with family history of pulmonary carcinoma and 38 cases(55.9%) with cigarette using history and family history of pulmonary carcinoma. 65 cases (95.6%) were with peripheral type of lung cancer, 3 cases(4.4%) were with central type. Among these patients, 38 cases with adenocarcinoma ,18 cases with squamous carcinoma. There were 27 cases (39%) in Stage I, 38 cases(55.9%) in Stage II and 3 cases(4.4%) in Stage III. There were 43 cases (63.2%) without symptom while 25 cases(36.8%) with symptom of pulmonary carcinoma.ConclusionMost of patients with asymptomatic pulmonary carcinoma were middle-aged male and were adenocarcinoma or squamous carcinoma in cell type and had closed relationship with cigarette using history and family history.

[Objective]Discussion on TCM name and pathogenesis of hypertension. [Method]To further explore and analyze the TCM name, location as wel as pathogenssis of hypertension after learning“Neijing”. [Result]Hypertension corresponding TCM diseases should be the veins bulge from“Neijing”. The location of the disease is heart,while it relates to internal organs. The main pathogenesis is that heart commanding blood abnormality. [Conclusion ]The traditional classical dialectical mode from the liver treatment of hypertension should be changed,the clinical treatment of hypertension is due to the heart according to the theory of pathogenesis which the heart commands blood vessels.Seeking the key reason is important to differential treatment of hypertension.

0.05). Conclusion: The tongue measurement with ultrasonic imaging is reproducible.

Objective To study the expression changes of matrix metalloproteinases (MMPs), protein kinase C (PKC) and nuclear factor-?B (NF-?B ) on the injured rabbit vascular smooth muscular cells (VSMC) transfected with tissue inhibitor of metalloproteinase-2 (TIMP-2) vector. Methods Lipofectin method was used to transfect TIMP-2 vector into VSMC, Western blot analysis to detect TIMP-2 peptides and zymography assay to determine MMPs. The activities of MMPs and NF-?B were detected by electrophoretic mobility shift assay (EMSA). The mRNA and protein expressions of PKC? were determined by RT-PCR and immunocytochemistry. Results The injured VSMC showed increased enzyme activity of MMP-2. There was very lower level expressions of PKC? and NF-?B in the normal VSMC but high in the injured VSMC. However, in injured VSMC transfected with TIMP-2 vector, the activity of MMP2/9 was suppressed and the expressions of PKC? and NF-?B decreased (P

Objective To explore the effect of early neuromuscular electrical stimulation (NMES) combined with sour taste therapy on dysphagia in the oral phase of acute/subacute ischemic stroke patients. Methods From January, 2013 to June, 2015, 90 patients with oral phase dysphagia after stroke were randomly assigned into NMES group (NM group), sour taste group (ST group ) and NMES combined with sour taste group (TO group) with 30 patients in each group. The swallowing function was evaluated with Functional Oral Intake Scale (FOIS) at baseline, three weeks and twelve weeks treatment. Results The change of FOIS was better in TO group than in ST group three weeks after treatment (P<0.01), and was better in TO group than in ST group and NM group twelve weeks after treatment (P<0.05). Conclu-sion An early application of NMES combined with sour taste therapy could improve the swallowing coordination, and reduce the the poten-tial complications in oral phase of acute/subacute ischemic stroke patients with dysphagia.

Objective To research the best extracting technology of simiaoyongan decoction.Methods As the total contents of chlorogenic acid and harpagoside to be evaluation indicator, adopted L9 (34 ) orthogonal design to select out the best extraction.The main determination was by HPLC method.Results Accounted by the total contents of the two composi-tions, the influential effects of the experimental results were extraction solvent >reflux times >the volume of extraction>the circumfluence time.Conclusion The best extracting technology is adding 10-fold times of 50% alcohol, percolating 1 hours, and three times.