OBJECTIVE:To observe the protective effect of Zhuyu annao pill on model rats against cerebral hemorrhage. METHODS:The experimental rats were randomized to 6 groups:blank group,model group,Angong liuhuang pill group,Zhuyu annao pill high,medium and low dosage groups. Cephalophyma model of rats were duplicated with rats' autoblood clot; the neurologic impairment change was recorded by integration method; the water content of the brain tissue was measured by dry-wet specific density method; meanwhile,the changes in hemorheology,blood coagulation (4 parameters),the blood coagulation time and the histology of the morphology of brain tissue were observed. RESULTS:In rats treated with Zhuyu annao pill (high,medium or low dosage) as compared with model group,no significant change was noted in the water content and neurological impairment; however,the pro-time (PT) was significantly prolonged(P

Gastric cancer stem cells are defined as a subset of gastric cancer cells that possess stem cell properties,including self-renewal and multiplex differentiation potential,and have crucial roles in tumor initiation,progression,invasion,metastasis and recurrence.Epithelial-mesenchymal transition (EMT) can facilitate the invasion and metastasis of cancer cells to distant tissues,and confer the metastatic cancer cells some abilities of stem cells.Several transcription factors of EMT,signaling pathways and microRNAs (miRNAs) are involved in the process of regulating gastric cancer stem cells.

Objective: To analyze the problems of large medical equipment allocation in our country, put forward the method and basis of scientific configuration. Methods: The current situation of large medical equipment allocation was analyzed by the methods of literature, statistics and analysis, in order to understand the operating costs and benefits and provide a scientific configuration tool when bringing in medical technology. Results: The configuration of large medical equipment has some problems, such as the uneven distribution of medical resources, equipment shortage, blind purchase of high-end equipment, low localization rate and so on. These problems will directly lead to the unreasonable allocation and the waste of medical resources. Conclusion:The configuration of large medical equipment must comply with the relevant provisions of the state by the HTA and other scientific methods, so as to improve the level of investment decision-making. It will make the use of medical resources efficiently and reasonably.

Objective To investigate the importance and method of early treatment of pelvic fracture combine with hemorrhagic shock.Methods Review the experience comes from emergent treatment of 74 cases of pelvic fracture combine with hemorrhagic shock in the department of emergent surgery.To study the characteristics,meaningful therapy and the affects to course of pelvic fracture.Results By effective early anti-shock treatment.Conclusion All 74 patients of pelvic fracture combine with hemorrhagic shock were safety recover from shock phase and get further treatment.

Objective To investigate the change of serum resistin levels in patients with obstructive sleep apnea hypopnea syndrome(OSAHS).MethodsPolysomnography(PSG)was performed in 58 patients with OSAHS and in 26 controls without differences in age and BMI.Serum resistin levels were measured by ELISA.Results(1)Serum resistin levels were significantly higher in patients with OSAHS than those in control group.(2)FPG levels were significantly higher in patients with OSAHS than those in control group.(3)TG levels were significantly higher in patients with OSAHS than those in control group.(4)Serum resistin levels were correlated positively with FPG in obesity patients with OSAHS、nonobesity patients with OSAHS and nonbesity controls group.(5)Serum resistin levels were correlated positively with TG in obesity patients with OSAHS、nonobesity patients with OSAHS and nonbesity controls group.(6)Serum resistin levels were correlated positively with BMI in OSAHS.(7)Serum resistin levels were correlated negatively with LSaO2 in OSAHS.(8)Serum resistin levels were correlated positively with AHI in OSAHS.ConclusionDetermination of serum resistin levels may be the predictors of the severity of OSAHS and it also contributes a lot taking measures to prevent and control the OSAHS complication occurrence and reduce the mortality.

Objective To analyze the development status worldwide of nutriology,so as to provide an orientation of its development in the future.Methods The professional articles published were retrieved by information research methods to predict the focal problem,and to provide the demand and presumption of nutriology development.Results In the past 5 years,some innovative concepts and technics were widely used in nutrition studies,such as nutritional genomics,nutritional proteomics and systematic biology,etc.These technologies made it possible to study the influences of nutrients on gene and protein expression,and reversely,the influences of gene changes in nutrient related disturbances in metabolism.Advances in clinical nutritional therapy provides patients with a variety of clinical treatment and the possibility of earty rehabilitation.At present,there are some problems in the fields of nutritional therapy in our country,such as a lack of resource of professional staff,imperfect education system,deficiency in understanding the importance of nutriology,and the lag of standardization and normalization of nutrition information.Conclusions The progress of nutriology in coming five years should lay stress on the parallel development of individual and nationwide health level.A series of studies in molecular biology and systematic biology will upgrade the national physical quality and the basic health condition of population nationwide.

[Objective]To study the proliferation inhibition effect of decetaxel combined with cisplatin on osteosarcoma cells,and the effect of the drugs during the course of chemotherapy for osteosarcoma.[Method]The effect of docetaxel and cisplatin alone or combined with osteosarcoma cell line HOS-8603 was examined by cell count,morphologic observation,flow cytometry(FCM).The animal model of human osteosarcoma was established in nude mice.They were given docetaxel or cisplatin alone and combined,and a blank control group was set up.Observations on the growth and pathologic markers of osteosarcoma were carried out and pathological examinations were made.[Result]Docetaxel and cisplatin either alone or combined inhibited significantlythe proliferation of osteosarcoma cells and induced obviously apoptosis,and the effect of the drags combined was more effective than that of the drugs alone,Growth inhibition ratio ≥59.34%(P

Objective: To establish a rapid approach to the detection and identification of Mycobacteria from lesions of patients with suspected Mycobacterial infections. Methods: Specimens were obtained from five patients suspected to have Mycobacterial infections. DNA extracted from clinical samples was amplified by nested PCR. The PCR products were digested with Hha Ⅰ, MboⅠ, and BstUⅠ restriction enzymes and applied to PAGE. The species of Mycobacteria were determined by restriction fragment length polymorphism (RFLP) analysis. Identification of Mycobacteria culture was also performed in 3 patients. Results: M. marinum was found in two patients diagnosed as swimming pool granuloma. M. tuberculosis was found in one patient diagnosed as infectious skin granuloma. All these 3 Mycobacteria were confirmed by Mycobacteria culture. A strain of M. tuberculosis and a strain of M. fortuitum were detected in remain two patients. Conclusion: The results above indicate that PCR-RFLP analysis is rapid and reliable in detection and identification of different Mycobacteria species from skin tissues. Application of this method will be helpful for early diagnosis and treatment of Mycobacteria skin infections.

Objective To investigate the possible role of mycobacterial infection in the pathogenesis of sarcoidosis.Methods DNA fragments of mycobacterial16S rRNA gene and the complex-specific insertion sequence IS6110of M.tuberculosis were detected by polymerase chain reaction(PCR)in formalin-fixed,paraffin-embeded skin biopsy specimens from12patients with sarcoidosis.Results Mycobacterial common sequence16S rRNA gene was found in6patients,among which5were further confirmed as M.tuberculosis,the other one was atypical mycobacteria.The IS6110DNA fragment amplified by PCR was confirmed by DNA sequencing.Conclusion The above findings suggest an aetiological role of mycobacterial infection,especially M.tuberculosis,in some patients with sarcoidosis.

ObjectiveTo investigate the effect of down-regulating epidermal growth factor receptor (EGFR) expression on Bleomycin induced pulmonary fibrosis in mice.MethodsForty 4 ～6 week aged C57BL/c male mice were randomly divided into control,bleomycin,bleomycin plus EGFR RNAi groups and RNAi negative control group.Bleomycin group were treated with bleomycin (3 mg/kg) by endotracheal injection on day 0,control group were treated with PBS.And bleomycin plus EGFR RNAi group were received EGRF siRNA plus bleomycin intratracheal administration.RNAi negative control group received negative EGRF siRNA plus bleomycin intratracheal administration.Mice were sacrificed 10 days after the treatments.Hydroxyproline (HYP) assay was performed in the lung tissue.The lung tissue slides were examined pathologically with H.E.staining,and EGFR mRNA expression was detected by reverse transcriptasepolymerase chain reaction (RT-PCR) technique.Western blot were performed to identify the protein level.of total EGFR and phosphorylated EGFR.ResultsHistological examination of lung specimens demonstrated that EGFR siRNA administration lessened lung fibrosis induced by bleomycin and significantly reduced HYP content (543.00±25.89 vs 900.73±31.77,P＜0.01).EGFR mRNA (0.31±0.05 vs 0.75±0.08,P ＜0.01) and protein expression(1.53±0.47 vs 2.56±0.37,P ＜0.01) in EGFR siRNA-treated mice was significantly decreased.The expression of phosphorylation of EGFR protein (1.78±0.35 vs 2.84±0.51,P ＜0.01) and EGFR protein in RNAi group was less than in bleomycin group.Conclusions EGFR RNAi reduced the BLM-induced lung fibrosis by inhabiting EGFR activation.