Objective To investigate the causes and prevetion of urethral stricture after transurethral resection of the prostate(TURP). Methods A retrospective analysis was carried out in 945 cases,who were treated by TURP in our hospital during February 2002-February 2008.Among the cases,21 patients(aged 62 to 84 years with a mean of 72.4 years) developed urethral stricture in 3 weeks to 24 months after the operation.In the cases,anterior urethral stricture was detected in 13 patients(61.9%),posterior urethral stricture was found in 5(23.8%),and bladder neck contracture was diagnosed in 3(14.3%).Results The pateints were cured by simple urethral dilation(13,61.9%),urethroplasty(2,9.5%),urethroplasty plus urethral dilation(2,9.5%),transurethral resection(1,4.8%),or electroresection of the bladder neck(3,14.3%).The patients were followed up for 6 to 36 months(mean,13 months),during which,all the 21 cases recovered without recurrence.The rate of urethral stricture after TURP was 3.7%(18/483) during February 2002 to August 2005,which was significantly higher than that between September 2005 and February 2008(0.6%,3/462;?2=10.292,P=0.001).Conclusions Unskilled operation and short indwelling time of urethral catheter after the surgery are the major reasons for urethral stricture after TURP.Regular follow-up and early treatment are the keys to cure of the disease.

Aim To investigate the cytotoxic activity of HDAC inhibitor Trichostatin A (TSA)combined with anticancer drugs targeting DNA on T24 bladder cancer cell line.Methods MTT assay was used to detect the inhibitory rate of TSA alone or combined with ADM, MMC and DDP respectively on T24 bladder cancer cell in cancer cell proliferation by administering TSA alone or combined with ADM, MMC and DDP respectively.Jin′s equation was used to evaluate the efficacy of drug combination. Results The growth inhibitory rate of TSA combined with ADM, MMC and DDP respectively was in a concentration-dependent manner. The synergism of TSA combined with MMC was most significant. When administered in lower or moderate concentration, TSA combined with ADM or DDP in lower or moderate concentration demonstrated synergic effect too.Conclusion HDAC inhibitor TSA enhances the cytotoxic activity of anticancer drugs targeting DNA on bladder cancer cells and is promising to be used in chemotherapeutic regimens for advanced bladder cancer.

Objective To investigate the effects of rosiglitazone,an agonist of proliferators activated receptor?(PPAR?),on expression of nuclear factor-kappa B(NF-?B)in peripheral blood mononuclear cells(PBMC)of multiple organ dysfunction syndrome(MODS) rats.Methods Forty male SD rats were randomly divided into four groups(n = 6 per group): vehicle control group,endotoxin(LPS) group,rosiglitazone(ROSI) pretreatment group,and PPAR-?antagonist GW9662 pretreatment group.Blood was taken 4 hours after operation,and mononuclear cells were separated.The expression of NF-?B p65in PBMC was detected by immunocytochemical method and image analysis was carried out.Results The expression of NF-?B p65 was low in vehicle control group.In LPS group,the expression of NF-?B p65 was significantly higher than that in vehicle control group(P0.05).Conclusion Rosiglitazone protected the MODS rats by inhibiting NF-?B activation.

Objective To observe the effect of psychological intervention on the anxiety of middle-aged and elderly patients with hypertension. Methods 378 middle-aged and elderly patients with hypertension accepted the intervention (health education and psychological intervention). They were assessed with the Zung Self-rating Anxiety of Scale (SAS) before and after the inertvention. Results The scores of the SAS in patients were higher than the Chinese normal. Female, younger, and higher eduction background were related to the anxiety symptom. The anxiety of the pateints were released after the intervention. Conclusion Health education and psychological intervention is effective on the anxiety of the middle-aged and elderly patients with hypertension at outpatient.

Objective To investigate the sleep disorder in the stroke inpatients for rehabilitation and research the strategy to improve the sleep disorder.Methods The sleep statuses of 168 stroke inpatients were investigated and recorded.The sleep disorder was set into 4 forms,insomnia,sleep interrupted,over-sleep and sleep reversed.Those with sleep disorder were given certain intervention,including improving the environments,eliminating body discomfort and mental disorder,medicines,and reset the sleep cycle.Their sleep statuses were evaluated again 2 weeks later.Results The incidence of sleep disorder was 60.7%,among which 32.3% for insomnia,22.5% for sleep interrupted,25.5% for over-sleep and 16.7% sleep reversed.It was 32.1% after intervention(P<0.05).Conclusion It is frequent of sleep disorder in stroke patients for rehabilitation.Comprehensive intervention can improve the sleep of them.

Objective To monitor the constituents and resistant tendency of bacterial pathogens isolated from diarrheal patients in our hospital form 1994 to 2005 to offer the basis for guiding epidemiologic study,vaccination research and clinical treatment. Methods Enteric pathogenic bacteria were cultured and identified to species,group and serotype with biochemical and serologic methods and the susceptibility of bacteria to antimicrobial agents were tested. Results Enteric pathogenic bacteria were isolated predominantly in male patients and mainly in children and youngsters. It reached a peak from July to September every year. Shigella spp.(75.11%) was the most frequendy isolated pathogens and followed by Vibrio spp.(12.7%),Salmonella spp.(6.28%),Aeromonas spp.(4.43%) and Escherichia coli(1.25%).During the period from 1994 to 2005,diarrheal pathogens had a trend of decrease especially Shigella spp.and Salmonella spp.. Of the 6329 isolates of Shigella spp., 75.62% was S. flexneri and S.soanei,S.dysenteriae and S. boydii constituted 23.98%,0.22% and 0.01% respectively.The sensitivity of different species,group or serotype to different antimicrobial agents was not the same.S.flexneri and Aeromonas spp. were highly resistant to most of antibiotics. However, S.sonnei and Vibrio spp.had good susceptibility to antibiotics tested except trimethoprim/sulfamethoxazole and ampicillin. Conclusion There are many species and serotypes of enteric pathogenic bacteria causing infective diarrhea and the distribution changes gradually in Beijing. The resistance rate of enteric pathogenic bacteria to antibiotics is not the same in different species and serotypes.so strict surveillance iS always needed.

AIM To determine the concentration of paeonol in the bulk drug and injection, a method of reversed-phase high performance liquid chromatography (RP-HPLC) was developed. METHODS Lichrospher C_ 18 column(4.6 mm?250 mm, 5 ?m)was used with methanol-acetonitrile-water(30∶40∶30)as mobile phase at a flow rate of 0.8 ml?min -1 . 20 ?l of sample was injected into the C_ 18 column where the temperature was 25℃. The detection wavelength was 274 nm. RESULTS The linear regression equation for paeonol was = 3 772.525 8 X- 0.747 4 (r = 1.000 0, n =5) under the linearity range from 0.02 mg?L -1 to 25.60 mg?L -1 . The average recovery was 99.87%. The relative standard deviations of the intra-day and inter-day were less than 4%. CONCLUSION The method is simple and rapid, which may be used for the determination of paeonol and its preparation for different purpose.

Objective To observe the curative effect of Huoxue-Tongluo-Zhitong ointment treating soft tissue injury,and the safety of application on skin.Methods The treatment group was treated with Huoxue-Tongluo-Zhitong ointment,while the comparison group was treated with Votalin Pill.Acute toxicity experiment,irritant experiment and hypersensitivity experiment of skin were performed on New Zealand white rabbits.Results Huoxue-Tongluo-Zhitong ointment decreased local edema and leukocyte effusion,accelerate the re-absorption of hematomas.On injury signs index,group A [ (0.32± 0.35),(0.29± 0.27) ] was better than group B [ (0.58±0.42),(0.91 ±0.34) ] (P＜0.05).The pathological process in group A and B was obviously reduced than group C (P＜0.05).In the neutruphil infiltration density measuring:compared with group C,group A and B both showed significant difference (P＜0.05),but there was no significant difference between group A and B (P＞0.05).Conclusion Huoxue-Tongluo-Zhitong ointment performed well on the treatment of soft issue injury,with no toxicity,irritation,and hypersensitivity of skin,as well as high safety of administration.

Objective To evaluate the effect of health education on the old male patients with primary hypertension.Methods 120 old male patients with primary hypertension were randomly selected from the whole group and educated with health knowledge related to blood hypertension, and the changes of life style and blood pressure after health education were recorded.Results Most of the patients changed their unhealthy life style. Among these patients, more than 80% were able to have a stable emotion, regular daily life, stop smoking and less drinking, there were 66% cases with a healthy diet. The systolic pressure of the patients declined significantly after health education (P<0.01).Conclusion The health education is an efficient way to control hypertension in the official-service outpatient department.

Objective To observe the vaccination reactions and immunogenicity of the application of lyophilized Vero cell rabies vaccine without adjuvant in a way of low-dose intradermal injection for post-exposure group. Methods Conducting post-exposure immunization for 256 persons with the class Ⅱ level exposure to rabies. Based on a randomized, single-blind principle, all subjects were divided into intradermal injection (ID) group (n= 128),injected 0.1 ml for each site in accordance with 0,3,7,28,90 d,2 sites,2 sites,2sites,1 site,1 site respectively, and intramuscular injection(IM) group(n= 128) in accordance with 0,3,7,14,28 d in full-volume (0.5ml) PVRV Deltoid injection. The local and systemic vaccination reactions were observed for the different injection ways. The indirect sandwich ELISA assay was used to analyze the antibody levels. Results For the intradermal injection group, the incidence rates for local redness and swelling, induration, pain, itch were 1.27%, 0.29% ,0.49% ,11.43% respectively,for the intramuscular group, the incidence rates were 1.09% ,0. 16% ,2. 81% ,1.41% respectively. From the point of systemic reactions,the incidence rates of fever,rash,headache,fatigue and weakness were 0.31 % ,0. 16% ,0. 31 % , 1.09% respectively in the intradermal injection group,and the rates were 0.31% ,0.31% ,0.63% , 1.09% respectively in intramuscular group. All the adverse effects often occurred following the 1st,2nd injection. The seroconversion rates for intradermal injection and intramuscular were 94.53% ,95.31% following 14 d immunization respectively,the rates were 96. 83% ,97.64% following 42 d immunization respectively. For the post-exposure group,no statistical difference in significance was found between the two seroconversion rates. Conclusion For the application of domestic lyophilized Vero cell rabies vaccine,its adverse reactions are mild,and immunogenicity is good.