50 caese of gastric carcinoma and 24 caese of superficial gastritis were studied by PAP immunohistochemical staining,with monoclonal antibody MG_7,MGd_1 for determining the associated antigen of gastric carcinoma. Among the 50 cases of gastric carcinoma,the positive rates of MG_7,MGd_1 were 92%,90%;whereas in the cases of superficial gastritis,those were 0% and 4% respectively.These results suggest a high specificity of MG7,MGd1 for gastric carcinoma.When the two monoclonal antibodies were used together,the positive rate was as high as 96%.So the combined use would be of more diagnostic value.

BACKGROUND: Induced response signal is blocked by the time of stimulation, showing some individual differences by special stimulation. Extracting induced response from magnetoencephalographic (MEG) data is important for understanding the function of human brain.OBJECTIVE: To apply independent component analysis (ICA) for overlapping multi-channel MEG signals so as to put forward a simple and effective method to analyze MEG data.DESIGN: A single sample analysis.SETTING: Electronic Engineering Department and Brain Scientific Research Center, Fudan University.PARTICIPANTS: The experiment was completed at Kansai Advanced Research Center of Japanese Communications Research Laboratory(CRL) in September 2002. One m ale healthy volunteer aged 23 years was selected from Tokyo Medical University of Japan, and other testees participated voluntarily.ed to process the 148-channel MEG data, especially for the extraction of eed independent components.MAIN OUTCOME MEASURES: Results of MEG data with ICA method.set. Most of the signal energy could be compressed in the first 30 principal components. In other words, the artifacts and evoked activations were exartifacts were detected and isolated to independent component 1, and the bursts should be detected in components 2, 3, 7 and 9. Beta bursts (13-30voked activation was obviously concentrated in component 5, which appeared a periodical waveform in response to the auditory stimulus.CONCLUSION: Interference source is separated from multi-channel MEG signals with ICA, then purified MEG data can be obtained. According to ICA, it is possible for research on cerebral nervous action to provide a new method by separating alpha wave, beta wave, eye movement artifacts and blinking. The auditory evoked response was successfully extracted from the multi-channel MEG signals using ICA.

There are Many gaps of medical psychology on course plans, teaching methods and examination methods in Chinese medicine institutions. Therefore, the teaching of medical psychology must be reformed. By improving course plans, strengthening multiple teaching methods and examination methods used, quality of teaching on medical psychology can be enhanced, and many persons with ability of traditional Chinese medical science can be educated to be abreast of bio-psycho-social medical model.

Hypofractionated conformal radiotherapy is capable to deliver much higher doses to the cancer than is possible with standard techniques. Recently there is data suggesting that the early stage nonsmall cell lung cancer ( NSCLC) which is not suitable to surgery is likely to benefit from this regimen, with low lung toxicity. Manyphase Ⅰ-Ⅱ studies showed that the patients with locally advanced NSCLC are well-tolerated to hypofractionated conformal radiotherapy. The model of radio-physic and relative clinical studies suggest that hy-pofractionation would not increase the risk of radiation pneumonitis compared to standard therapy.

Objective To set up assessment indexes of nursing process quality in hospitals that are scientific, practical and in keeping with the times so as to provide hospital departments in charge of nursing with the basis for reasonable assessment and scientific decision-making. Methods Typical investigations were conducted to understand the content and methods of the current assessments of nursing process quality in hospitals; the basic scope of the assessment indexes was determined through meeting discussions; the indexes were selected, verified, and established by means of brainstorming and the Delphi method; field surveys were conducted to assess and analyze the nursing process quality in 25 departments. Results A system of comprehensive assessment of nursing process quality in hospitals was established. It covered four categories, viz. nursing services, nursing control, nursing skills and nursing safety, and decomposed into assessment indexes of different levels, with the overall structure consisting of 1 overall goal, 4 first-level indexes, 12 second-level indexes, 5 third-level indexes and 290 subindexes. Conclusion The new system of indexes, which stresses the idea of nursing services, highlights the quality control principle of focusing on the patients,and is characterized by clear-cut structure and easy operability, provides a reliable guarantee for scientifically and rationally assessing nursing process quality.

Intervertebral disc (IVD) degeneration is considered to be the main cause of low back pain (LBP),however,there are lack of long-lasting and effective methods of clinical treatment.Tissue engineering technique based on stem cells becomes an essential research direction on repair of IVD degeneration at present,and its effectiveness and feasibility have been confirmed,but it is difficult to maintain the sufficiency and vitality of stem cells in IVD.Previous studies showed that stem cells existed naturally in IVD,and stem cells from stem cell niche could migrate to IVD physiologically to maintain the IVD environment balance under the adverse microenvironment.Unfortunately,these behaviors cannot preclude IVD degeneration.Therefore,theoretical basis for the regeneration of nucleus pulposus (NP) in situ can be obtained from studying the mechanism that the endogenous repair failure during IVD degeneration,the cell death and the migration of stem cells in IVD,and the key regulatory targets to sustain the quantity and quality of the stem cells.Although there have been few researches to study the mechanism of the cell death and the migration of stem cells in IVD so far,studies demonstrated that the major inducing factors of IVD degeneration (pressure and hypoxia) could decrease the number of NP cells by autophagy,apoptosis and necroptosis,and chemokines and their receptors played a critical role in the migration of mesenchymal stem cells.These researches provide a clue for studying the mechanism of endogenous repair failure during IVD degeneration.We reviewed the current research situation and progress of the mechanism that endogenous repair failure during IVD degeneration in the following articles.First,we exhibited the potential of IVD stem cells in IVD degeneration repair.Second,the effect of the adverse microenvironment (pressure,hypoxia,etc) on the migration of IVD stem cells was discussed.Third,the mechanism of the stem cell death,autophagy,apoptosis and necroptosis under the adverse (pressure,hypoxia,etc.) microenvironment,and the correlation between the IVD stem cells migration and autophagy,apoptosis and necroptosis was studied.And then tissue engineering of NP was also discussed to achieve the endogenous repair of IVD degeneration.These studies will provide an innovative research direction on endogenous repair and a new strategy of early therapy for IVD degeneration.

ObjectiveIn order to understand changes of immunological response under different psychic state. MethodsMice were divided into two groups,male and female group, 1: 2, were feeded together for together group, anther was that mice, male, were feeded solely for thesolely group. Male mice in two groups were immunized with sheep red blood cell(SRBC). Titer of antiboty against SRBC and IL -2 in serum wasdetected. Results The titer of antibody against SRBC in together group was higher than that in solely group. The concentration of IL - 2 was lowerin the solely group comparing with the together group. ConclusionIt was suggested that psyxhological status had impact on immune system.

The studies on pharmacoki-netics of Quercetin in rabbits were carried out by UV-spectrophotometry. The experimental results were shown that after 10mg ? kg-1 of Quercetin iv administration in rabbits, the curve of plasma concentration - time was shown to fit an open two compartment model. The pharmacokinetic parameters were as follow:T1/2 (?) = 2. 91 ? 1. 36min,T1/2(?) = 183. 78?82. 67min,Vd=0. 624?0. 225L ???kg-1,CL = 3. 15 ?2. llml ? kg-1 ? min-1. The bioavailability (F) of Quercetin after 10mg ? kg-1 ig in rabbits was 42.7% and Cpk was 10. 9mg ? L, tpk was 60min.After 10mg ? kg-1 iv administration in rabbits,Quercetin was rapidly eliminated from the blood. The compound and its metabolites were exereten by kidney and bile.

Objective To explore the effect of compound sophora flavescens injection on intraperitoneal chemotherapy of cisplatin in malignant ascites of gastric cancer patients and its influence on immune function.Methods 90 cases with malignant ascites of gastric cancer failures were researched,who from May 2014 to May 2016.According to random number table, they were divided into observation group and control group, 45 cases in each group.The control group was treated with intraperitoneal chemotherapy of cisplatin alone, and the treatment group was treated by intraperitoneal chemotherapy with compound Kushen injection combined with cisplatin.The two groups of patients before and after treatment, compared the level of immune function indicators, while observing the two groups of patients after treatment, short-term efficacy, quality of life improvement and adverse reactions.Results Observation group of patients with efficient and control rates were higher than the control group patients, but no obvious difference between groups.The stability factor of 55.55%of patients with observation group was obviously higher than that of control group 22.22%of the patients, the clear difference between groups (P<0.05).Before the treatment, two groups of patients of CD3 +, CD4 +, CD8 +and CD4 +/CD8 +T cell subsets and NK cells was no significant difference.After treatment, two groups of patients of CD3 +, CD4 +, CD4 +/CD8 + T cell subsets and NK cells were significantly increased than before treatment ( P<0.05 ) , and CD8 + than before treatment significantly reduced ( P <0.05 ) , while the observation group of CD3 +, CD4 +, CD4 +/CD8 +T cell subsets and NK cells was significantly higher than that of control group ( P <0.05 ) , and CD8 + was significantly lower than control group (P <0.05).The control group in the improvement of quality of life of patients with effective rate of 35.55%compared with 80.00% of the observation group of patients, significantly lower, more obvious differences between groups (P <0.05).Observation group of patients the incidence of gastrointestinal reaction and liver function abnormalities were 0.00%, 8.88%, significantly lower than the control group patients were 11.11%, 22.22%, there exists significant difference between groups (P <0.05).Fatigue, nausea, vomiting and 2 groups of patients compared the incidence rate of no significant difference.Conclusion The combined use of compound Kushen injection combined with cisplatin intraperitoneal perfusion chemotherapy in patients with malignant ascites of gastric cancer for treatment,effect is remarkable,Can effectively improve the patient's T cell subsets and NK cells,Improve the patient's immune function.

Objective To investigate the expression of CD133 protein in primary lesions of hepatocellular carcinoma (HCC) and its clinical significance.Methods The expression of CD133 protein in 190 patients with HCC was detected by immunohistochemical staining ABC method.The correlation of CD133 protein expression with the clinicopathologic parameters and features after operation was analyzed.Results The expression positive rate of CD133 protein in cancer tissues was 22.1％ (42/190).There was significant correlation between the expression of CD133 protein and tumor differentiation (P ＜ 0.001),micro vessel invasion (P =0.016) and hepatitis B virus infection (P =0.024).Univariate analysis of factors demonstrated that differentiation level,lymph node,macro vessel invasion,micro vessel invasion,TNM stage and CD133 expression were correlated with disease-free survival after surgery (all P ＜ 0.05).Multivariate analysis of factors demonstrated that both CD133 expression and micro vessel invasion were independent prognostic factors of disease-free survival after surgery.Prognostic analysis demonstrated that the disease-free survival of CD133 positive group was significantly lower than that of CD133 negative group.Conclusion The CD133 protein expression in HCC tissues is related with development,metastasis and prognosis of HCC.