Objective:To explore the application value of multislice spiral CT scanning on abdomen for the detection of complication of acute pancreatitis.Methods: 120 patients with acute pancreatitis were divided into observation group (70 cases) and control group (50 cases) according to different detection method. The patients of control group were applied basic clinical detections included X-ray radiography and ultrasound imaging, while patients of observation group were supplemented CT detection besides these detections of control group. And situation of complication of patients in two groups were observed.Results: The positive rate of complications, which involved pancreatic pseudocyst, cellulitis, complications of gastrointestinal tract, pancreas hemorrhage and pancreatic ascites, in observation group was 97.14%, that was significant higher than that (62.0%) of control group (x2=24.74,P<0.05). Among 20 patients with pancreatic pseudocysts, there were 9 patients located at tails of pancreases, 6 patients located in bodies of pancreases, 3 patients located in small omental bursas, 2 patients located at heads of pancreases, and the CT images of them showed quasi-circular liquid form density lesions with clear boundaries appeared in lesion field. The CT images of cellulitis showed the volume of pancreas increased and the boundaries were fuzzy, and a larger range of density lesions with irregular soft tissue shape appeared in its periphery. CT detetions of gastrointestinal complications showed fuzzy point-shaped, strip-shaped and flake-shaped soft tissue-styled density shadow appeared in gastrointestinal tracts. The CT image of pancreatic ascites showed intraperitoneal fluid density shadow.Conclusion: Abdominal CT scan applied on patients with acute pancreatitis can increase the positive rate of complications and may be used as preferred detection method for clinical evaluation on the complications of acute pancreatitis.

AIM: To study the inhibitiory effects of Fuerkang Injection (sweet worm wood oil, glycyrrhetate and Vitamin A acetate). and its ingredients on SMMC-7721 and NB4 cell lines, in order to understand its antitumour spectrum and reasonability of its composition. METHODS: SMMC-7721 and NB4 cell lines were treated with Fuerkang Injection and its ingredients under the same concentration for 48 h, adfiamycin was used as standard com-parison,inhibitiory effect on growth of two cell lines was detected by MTT method. RESULTS: At 1.260 μg/mL, the inhibition rate of Fuerkang Injection and its decomposed recipes preparations 1,2,3 to SMMC-7721 cell lines were 54.78%, 55.49%, 53.18%, 53.79%, hemi-inhibitory concentration IC_(50) were 0.415,0.376,0.715 and 0.636 μg/mL,respectively. The inhibition rate of NB4 cell lines were 88.6% ,82.5% ,77.9% and 76.9% ,hemi-inhibitory concentration IC_(50) were 0.091,0.108 1,0.084 and 0.120 μg/mL. CONCLUSION: Fuerkang Injection and its ingredients have significant inhibitory action on SMMC-7721 and NB4 cell lines,each component herb in the compound preparation has synergetic effects.

AIM: To study the inhibitiory effects of Fuerkang Injection (sweet worm wood oil,glycyrrhetate and Vitamin A acetate) and its ingredients on SMMC-7721 and NB4 cell lines,in order to understand its antitumour spectrum and reasonability of its composition. METHODS: SMMC-7721 and NB4 cell lines were treated with Fuerkang Injection and its ingredients under the same concentration for 48 h,adriamycin was used as standard comparison,inhibitiory effect on growth of two cell lines was detected by MTT method. RESULTS: At 1.260 ?g/mL,the inhibition rate of Fuerkang Injection and its decomposed recipes preparations 1,2,3 to SMMC-7721 cell lines were 54.78%,55.49%,53.18%,53.79%,hemi-inhibitory concentration IC_50 were 0.415,0.376,0.715 and 0.636 ?g/mL,respectively.The inhibition rate of NB4 cell lines were 88.6%,82.5%,77.9% and 76.9%,hemi-inhibitory concentration IC_50 were 0.091,0.108 1,0.084 and 0.120 ?g/mL. CONCLUSION: Fuerkang Injection and its ingredients have significant inhibitory action on SMMC-7721 and NB4 cell lines,each component herb in the compound preparation has synergetic effects.

The effect of maltol to prevent erythrocyte from auto-oxidation is presented. When erythrocytes were incubated at 37 ℃ for 24 h in vitro, oxyhemoglobin was decreased, methemoglobin, superoxide freeradical, lipofuscin and Heinz's body were increased as well as membrane proteins were changed. These reactions could be inhibited by maltol.

Objective To evaluate the performance of artificial neural network(ANN) models for predicting ovarian malignancy in the patients with adnexal masses by using B-mode and color Doppler ultrasonography.Methods The data of 180 patients with adnexal masses were randomly divided into training and testing subsets.The training subsets were used to screen out significant single parameters and to compute the optimum statistic equations and to train the ANN.The testing subsets were used to estimate the performance of each of the two models in predicting ovarian malignancy.Results Thirteen of seventeen parameters were significant to diagnose malignancy in adnexal masses by single analysis(P

Objective To explore effects of monitored anesthesia care (MAC) on gastrointestinal endoscopy.Methods All patients who underwent gastrointestinal endoscopy in Peking University People's Hospital from September 2010 to September 2015 were analyzed retrospectively and divided into two groups:the MAC group and control group by usage of MAC.Success rates of removal of foreign bodies by upper gastrointestinal endoscopy were compared between two groups during this period.Detection rates of ectopia of gastric mucosa in upper esophagus were compared between two groups from September 2014 to September 2015 when esophagogastroduodenoscopy was performed.The monthly cases of gastrointestinal endoscopies were compared between the two groups,which were separately collected from one MAC operating table and one routine operating table from September 2014 to September 2015.Results The success rate of removal of foreign bodies in the MAC group was higher than that in the control group (100.0％ VS 89.7％,x2 =11.737,P=0.001).The detection rate of ectopia of gastric mucosa in upper esophagus in the MAC group was higher than that in the control group(4.3％ VS 1.7％,x2 =58.751,P＜0.001).The monthly cases of gastrointestinal endoscopies in the MAC group was higher than that in the control group(350.2±36.2 cases VS 213.2±27.9 cases,t =17.591,P＜0.001).Conclusion The use of MAC for gastrointestinal endoscopy improves operating success rate,accuracy and operating time.MAC in gastrointestinal endoscopy can be recommended.

Objective To realize the status in the implementation of three industry standards of central sterile sup-ply department (CSSD),and provide a scientific basis for carrying out of the standards. Methods According to three standards,investigation forms were designed by specialists,written survey on 365 hospitals in 9 provinces and field investigation on 1 5 hospitals in 3 provinces were performed,the implementation of three standards were investi-gated.Results Of 365 hospitals,the number of provincial and ministerial level,municipal level,and county level hospitals were 90,87 and 188 respectively. More than 94% of hospitals established CSSD management system and regulations,>90% of hospitals met the requirements of CSSD layout;All hospitals were equipped with pressure steam sterilizer,all levels of hospitals basically equipped with the necessary equipments and facilities;CSSD respon-sible officers of 94.52% (345/365)of hospitals participated in training on standards;69.61% (252/362)of hospi-tals were using or developing CSSD information systems;>92% of the CSSD responsible officers considered that three standards played an important role in facilitating centralized management,and improving the quality of clean-ing,disinfection and sterilization.Conclusion Hospitals need to strengthen the management and training on stand-ards of CSSD,management of loaner instruments and development of information system need to be standardized.

Objective: To investigate the mechanism that glutamine downregulates the cytokine expression in lipopolysaccharide-stimulated human peripheral blood mononuclear cells.Methods:PMBC were extracted from healthy volunteer by density gradient centrifugation,and stimulated by lipopolysaccharide.The release of TNF-α and IL-10 was analyzed via enzyme-linked immunosorbent assay and p-P38 via western blot. Results:Gln led to an increase in HSP70 expression,and decreased TNF-α、IL-10 release at 4 h after LPS stimulation.The expressions level of TNF-α、IL-10 and P-P38 were decreased after using SB203580.Conclusion:These results indicate that the effect of Gln could attenuate cytokine release in PBMC and might may be related to the inhibition of P38 MAPK.

BACKGROUND:Long-term use of corticosteroids (hereinafter referred to as hormone) after renal transplantation could obviously lead to adverse reactions. Immunosuppressive regimen with less and no hormone has been a hot focus in the study of renal transplantation al over the world. However, reduction or withdrawal of hormones has a certain risk. At present, there is no unified scheme. Because urine protein can be immediately detected after tubular injury, to monitor urine protein can find the renal dysfunction after transplantation in recipients undergoing renal transplantation, which can gain time for clinical therapy. OBJECTIVE:To discuss the influence of hormone (prednisone) removal on the occurrence of urine protein in recipients undergoing renal transplantation. METHODS:A total of 35 recipients undergoing renal transplantation after removal of prednisone received immunosuppressive regimen of cyclosporine A or tacrolimus+mycophenolate mofetil bivalent. Initial dose of prednisone was 30 mg/d, and then gradual y reduced by 5 mg per week, and withdrawn at 1 month after renal transplantation. There were 16 cases in cyclosporine A group and 19 cases in tacrolimus group. Urine protein was measured and quantified at 3, 6, 12 and 24 months after renal transplantation and 3, 6 and 12 months after addition of prednisone in both groups. Simultaneously, serum creatinine, fasting glucose, body mass increases, the rate of acute rejection, infection, patient/graft survival at 2 years after renal transplantation and urine protein at 24 hours before and after adding hormone were recorded. RESULTS AND CONCLUSION:For the two groups, urineα1-microglobulin started to rise after 6 months of removal of prednisone. Urinary microalbumin, urinaryα1-microglobulin, and urinary transferrin ascended obviously at 12 months. Urinary protein was positive in five cases of cyclosporine A group and in three cases of tacrolimus group. At 24 months, urinary microalbumin, urinaryα1-microglobulin, urinary transferrin and urinary IgG ascended obviously. Urinary protein was positive in cyclosporine A group with 11 cases and in tacrolimus group with 10 cases. 24-hour urinary protein quantity was more than 1 g in every case. On this base, we made the patients to take more prednisone for 6 months, so urineα1-microglobulin and urinary microalbumin began to descend. Each group had one case of positive urinary protein turning to negative. Twelve months after the adjustment of the prednisone, urinary microalbumin, urinaryα1-microglobulin, and urinary transferrin descended respectively. Positive urinary protein turned into negative:in cyclosporine A group with two cases and in tacrolimus group with three cases. 24-hour urinary protein quantity was around 0.7 g. Two years after renal transplantation, serum creatinine and acute rejection rates were higher in the cyclosporine A group than in the tacrolimus group (P<0.05). No significant difference in fasting glucose, body mass increase, infections, and patient/graft survival was detectable between both groups. Results suggested that removal of prednisone greatly affected urine protein in recipients undergoing renal transplantation. In particular, at 2 years after renal transplantation, urinary microalbumin, urinaryα1-microglobulin, urinary transferrin and urinary IgG ascended obviously, and the security needs further research.

ObjectiveTo explore the role of autofluorescence bronchoscopy in lung cancer operation and the meaning of choice lung cancer operation mode.MethodsTo retrospectively analyze 32 non-small cell lung cancer patients.Before lung cancer operation,white light bronchoscopy (WLB) and autofluorescence bronchoscopy (AFB) had been done routinely.Compared the different invasive tumor conditions by WLB and AFB,operation modes were decided by the edge of the tumor which were proved by biopsy pathology.ResultsIn 32 cases,19 cases underwent pulmonary lobectomy.One case underwent carinal resection and reconstruction.8 cases underwent sleeve lobectomy.4 cases underwent other therapy because of tumor airway metastasis.In 8 cases which underwent sleeve lobectomy,3 ca ses were found by WLB and AFB together,5 cases were found only by AFB.In 4 cases who had no operation chance,2 cases were found by WLB and AFB together,2 cases were only by AFB.The sensitivity for the detection of bronchial premalignant lesions was extremelyhigher withthe addition of AFB than WLBalone ( P ＜ 0.05 ).ConclusionAutofluorescence bronchoscopy is a safe and efficient technique which could improve the sensitivity of diagnosis in lung cancer than WLB.It is important to select operation mode.