Purpose:To evaluate the accuracy of diagnosing the gastrointestinal vascular malformation and the value of treatment of the hemorrhage of the interventional radiology.Materials and Methods:The superior mesenteric arteriography or/and the inferior mesenteric arteriography or arteriography of coeliac axis were performed by applying Seldinger's techniques in 22 cases with the gastrointestinal bleeding which were suspicous of the gastrointestinal vascular malformation,otherwise were negative or equivocal in di- agnosis by performing with non-interventional study.Results:Focci were detected in 31 arteriographies (22 cases)with the positive gastrointestinal vascular malformation.Operative treatment were performed in 14 of 22 cases,with confimation of the vascular malformation pathologically.Conclusion:The se- lective coeliac arteriography may be a reliably unique method in detection of the gastrointestinal vascular malformation.

The fluorescence characteristics of reaction products of sparfloxacin with halide (F, Cl, Br, I) have been studied. It was found that sparfloxacin was oxidized by nitrous acid then reacted with halide further to formstrong fluorescence substances in acid media. Their fluorescence intensitites enhanced with aggrandizing of atom radii of halides, which could emit the strong fluorescence 58～151 fold more than that of sparfloxacin itself. Thus a new sensitive method for the direct determination of sparfloxacin in human urine by derivative-synchronous fluorescence was presented. A plausible mchanism was proposed to explin this behavior.

Objective:To investigate the recognition degree and attitude to adverse drug reaction (ADR) reports among the healthcare professionals in our hospital.Method:Through random cluster sampling,the doctors,nurses and pharmacists in our hospital were interviewed on the spot.The questionnaires were input by EPI6.04 and analyzed by soft- ware SPSS11.5.Result:1077 eligible questionnaires were obtained and the recovery was 89.8%.Our bealthcare profes- sionals had a good recognition of basic ADR knowledge.The pharmacists had a better recognition degree than doctors and nurses.The better academic background the healthcare professionals had,the higher their score of recognition was,and there was a significant difference among them.There was no significant difference among the Scores of the healthcare profes- sionals with different working years,and nor was there among the scores of them with different professional titles.The atti- tude to ADR reporting was entirely right,but there were some wrong ideas.Conclusion:It is urgent to provide more train- ing courses for healthcare professionals so as to raise their consciousness of ADR reporting,ability of discrimination and lev- el of monitoring and avoid serious consequences caused by ADR.

To ensure the sooth progression of resident standardized training, the Nineth Peo-ple's Hospital affiliated to Shanghai Jiao Tong University School of Medicine made the following explo-rations for structure organization and rule and regulation establishment: teaching staff construction , student recruiting procedures, training time, training plan and content, examination management and training quality control. It has played a promoting role for the construction and development of resident standardized training base.

0,most symptoms of ADR were reported for the first time with the homogeneous literature ratio being 0.The predominant outcome of severe ADR were hospitalization or delayed hospitalization course,followed by increased risk of death or patients' permanent or distinct disabling.CONCLUSION:New and severe ADR should be given close attention in the clinic to guard against severe ADR.

Background Acute lymphoblastic leukemia (ALL) and chemotherapy can cause immune imbalance,and gaseous molecule hydrogen sulfide (H2S) can participate in the process of immune response.This study aimed to investigate the immune regulation of H2S in pediatric ALL.Methods Children (n=78) with ALL admitted during 2010-2013 were included in this study.Two blood samples were collected in period of before chemotherapy,bone marrow remission and two days after chemotherapy,respectively.Serum contents of H2S and cytokines,including interleukin-1β (IL-13),interleukin-2 (IL-2),interferon-γ (IFN-γ),tumor necrosis factor (TNF-α),interleukin-4 (IL-4),interleukin-6 (IL-6),interleukin-10 (IL-10) and macrophage inflammatory protein-1α (MIP-1a),were detected using ELISA method.Stepwise regression was used to analyze the correlation between H2S and cytokines.Furthermore,human Jurkat cells were cultured in vitro,and nucleoprotein of Jurkat cells and peripheral blood mononuclear cells (PBMCs) were collected,contents of cystathionine γ-lyase (CSE) and certain cytokines were measured by Western blotting.Results Serum concentrations of H2S,IL-13,IL-6,IL-10 and MIP-1α in children with ALL were increased significantly (P ＜0.01),while concentrations of IL-2,TNF-α,IFN-γ and IL-4 decreased obviously (P ＜0.01).In patients after chemotherapy,concentrations of H2S and IL-10 were decreased significantly (P ＜0.05),but IL-4 and IFN-γ concentrations increased markedly (P ＜0.05).At remission stage,H2S,IL-13,IL-4,IL-6,IL-10 and MIP-1α concentrations were further decreased markedly (P ＜0.05),but concentrations of IL-2,TNF-α and IFN-γ increased again (P ＜0.05).Protein contents of CSE,IL-10,IL-4 and IL-2 of PBMCs also increased markedly in children with ALL.Moreover,changes of CSE protein contents of PBMCs were consistent with serum H2S contents,and there were significant correlation between H2S and certain cytokines based on stepwise regression analysis.Furthermore,compared with those of PBMCs group,in vitro study indicated that Jurkat cells of H2S group expressed IFN-γ,IL-10,IL-4 and IL-2 protein increased obviously (P ＜0.05),while IL-4,IL-2 and CSE expression of PPG group decreased markedly (P ＜0.05).Conclusion Gaseous molecule H2S might participate in the process of immune regulation in pediatric ALL through modulating transcription and expression of cytokines.

Humans ; Serum ; chemistry ; Spectrophotometry, Ultraviolet ; methods ; Sulpiride ; blood

Objective To study the difference of expression of proteins in pancreatic cancer and adjacent fissue.Methods Proteomes of eight pairs of pancreatic ductal adenocarcinoma tissue samples and adjacent tissue samples were obtained by high resolution two-dimensional gel electrophoresis(2-DE).Comprehensive analyses of proteins were focused on total protein spots exhibiting statistical alternations between the two groups.Protein identification was done by peptide mass fingerprinting with tandem mass spectrometry(MS/MS).In addition,Western blotting and immunohistochemistry were performed to verify the expression of certain candidate protein.Results A total of 28 protein spot-features were found to be significantly increased and 17 significantly decreased in tumor tissues.Thirty of these protein spots were identified,which included enzymes,antioxidant proteins,signal transduction proteins,calcium-binding protein,structural proteins,chaperones and others.Western blotting and IHC further validated up-regulated expressions of one candidate protein(annexin II)in tumor tissues.Conclusions The analysis of proteomics with 2-DE on human tissue is a useful method for discovering valuable cancer marker candidates.These differential expressed proteins may serve as biomarkers for early detection and therapeutic targets to pancreatic cancer.

Objective To explore the feasibility of building a new model of free superficial epigastric artery perforator flap with New Zealand white rabbits.Methods Twenty-five healthy New Zealand white rabbits were used.Five rabbits were dissected to assess the vascular territory,the source and dimension of the superficial epigastric.According to the anatomic results,the superficial epigastric artery perforator flap were designed and harvested with 6 cm× 3 cm in the abdominal,anastomosis had been done between femoral artery/vein and carotid artery,jugular venous to build an animal model of transplantation of epigastric artery perforator flap.The donor sites were directly closured.Results Superficial epigastric artery originated from the proximal femoral artery,and the diameter of superficial epigastric artery was (0.15 ±0.02) mm,2-3 perforators support the areas of the skin of the lower abdominal wall.A rabbit died in the surgery because of anesthesia,the other animals were alive.The results showed that all flaps were survived in the experiment except a flap lost due to venous crisis.Conclusion The experimental model of free superficial epigastric artery perforator flap can be built in New Zealand white rabbits.It is a simple,practical and reliable model for future physiologic and pharmacologic studies.

Aim To observe mesenchymal stem cell differentiation into osteoblast induced by TSPG, and explore how TSPG enhances the promotion of hemato-poiesis of osteoblast differentiation from mesenchymal stem cell. Methods MSCs were cultured by TSPG combined with osteoinductive medium. The cellular vi-ability of proliferation was detected with MTT assay. The content of alkaline phosphatase in the cultural su-pernatant was tested with pNPP assay. The ability of MSCs to form calcium nodes was also observed after a-lizarin red stain. The protein expression of RUNX2 was analyzed with Western blot. The content of cytokines associated with hematopoiesis was tested with Elisa as-say. The ability of promoting hematopoiesis was detec-ted with hematopoietic colony forming assay. Results Both MTT and pNPP assay showed that optical den-sity ( OD) values were increased in response to TSPG treatment in a dose-dependent manner. The mineraliza-tion formation ability was enhanced with TSPG-treat-ment. Meanwhile, the expression of RUNX2 protein was up-regulated in TSPG-treated cell. Moreover, the content of cytokines associated with hematopoiesis and the number of hematopoietic progenitor colony were in-creased by TSPG-treatment compared with the control group. Conclusion TSPG could induce MSCs differ-entiation in to osteoblast and enhance the effect of oste-oblast differentiation from MSCs on promoting hemato-poiesis.