Objective To investigate the expression differences of β‐catenin ,Her‐2/Neu and ki‐67 in different pathological features of gastric cancer and their role in the occurrence ,progression ,invasion and metastasis of gastric cancer invasion and metas‐tasis .Methods The expression of β‐catenin ,ki‐67 and Her‐2/Neu in 150 gastric cancer tissue samples(observation group) and 70 normal gastric mucosal samples were detected by adopting the immunohistochemistry method ,and the relationship between the ex‐pression of β‐catenin ,ki‐67 and Her‐2/Neu with the pathological features of gastric cancer was analyzed .Results Theβ‐catenin ,ki‐67 and Her‐2/Neu positive rate in the observation group was significantly higher than that in the control group (χ2 =30 .51 ,63 .74 , 11 .38 ,P<0 .01);the differentiation degree affected the expression of β‐catenin ,ki‐67 and Her‐2/Neu expression (χ2 =15 .82 ,8 .61 , 8 .11 ,P<0 .01);the lymph node metastasis affected the expression of ki‐67 and β‐catenin (χ2 = 6 .26 ,P< 0 .05;χ2 = 7 .36 ,P<0 .01);the invasion depth affected the expression of β‐catenin and ki‐67(χ2 =11 .34 ,P<0 .01);TMN affected the expression of ki‐67 and Her‐2/Neu(χ2 = 10 .85 ,14 .24 ,P< 0 .01);the β‐catenin and ki‐67 expression showed positive correlation .Conclusion β‐catenin ,ki‐67 and Her‐2/Neu may reflect the proliferation ,invasion and metastasis situation of tumor cells and their detection con‐duces to the judgment of prognosis .

HL7 is a widely-applied international standard for medical information exchange. With a HL7 data interface applied to a mainstream fully automatic biochemical instrument, this paper implements the information exchange between biochemical instruments and LIS following HL7 standard. This paper can be referred to when HL7 applied in other instruments.

[Objective]To investigate the endogenous erythropoietin expression of neural stem cells(NSCs) of rats after hypoxia.[Method]We used a rat NSCs culture model.EPO expression after 4 hours of hypoxia was studied.It was examined again following 12 hours of hypoxic lethal insult which was applied various times after hypoxic preconditioning.EPO expression was studied by western blotting.[Result]Following the preconditioning,expression of EPO increased immediately and reached a peak 4 hours later.It lasted till 8 hours later.While applied the lethal hypoxia,EPO expression of cells in preconditioning 24 hours group were was significantly increased than that in preconditioning 4 hours group.[Conclusion]The hypoxic preconditioning can lead an increased endogenous EPO expression and decrease the threshold of EPO expression against subsequent lethal stimulation during a certain period.

[Objective]To observe the growth status and osteogenic potential of gene transferred mesenchymal stem cells with Ad-BMP-2 planted on allogenetic acellular and decalcified bone matrix served as tissue engineering scaffold.[Method]Rabbit mesanchymal stem cells transferred with Ad-BMP-2 were seeded on allogenetic acellular and decalcified bone matrix,the growth status of gene transferred MSCs on the scaffold was observed by scanning electron microscopy and the ostengenic potential was observed by testing contents of ALP and BGP and secretion of Collagen I in the culture supermatant.[Result]The MSCs transfected with Ad-BMP-2 grew well on the tissue engineering scaffold,the osteogenic ability of the gene transfected MSCs showed great significant difference compared with that of the control group.[Conclusion]The MSCs transfected with Ad-BMP-2 can grow well on the acellular and decalcified bone matrix and the osteogenic potential can be great improved by gene transfection.

Objective To analysis the flash electroretinogram (FERG) of diabeic retionpatby patients. Method 90 cases of DR patients were selected. According to the partial signs and the overall symptoms, the patients can be divided into three syndromes - dry heat by yin deficiency, deficiency of both qi and yin, deficiency of yin and yang. FERG were detected. Result There was close relation between the occurrence and development of DR and the evolution of the syndrome from yin deficiency to yang involved by deficient yin. Retina actional potential will change along with the change of syndrome. Conclusion The retina actional potential which recorded by FERG can be used as an objective criterion of syndrome differentiation, thus we can establish a diagnosis and treatment system of DR.

Objective To evaluate the efficacy of target controlled infusion(TCI) of remifentanil and propofol in patients at different ages undergoing gynecologic laparoscopic surgery.MethodsA total of 60 patients(ASA Ⅰ-Ⅲ) undergoing elective gynecologic laparoscopic surgery were divided into two groups according to their age(Group Ⅰ:

Objective To study the association between the CTLA-4 gene polymorphism and Graves disease.Methods A total of 120 unrelated patients with Graves disease southern Han nationality of China were recruited from clinics in the Department of Endocrinology and Metabolism,the First Affiliated Hospital of Sun Yat-sen University from 2001 to 2003.Ethnically matched 123 control subjects with no history of autoimmune disease were from Guangdong.We investigated the polymorphism of the cytotoxic T lymphocyte antigen 4 gene on chromosome 2q33.1 microsatellite markers of CTLA4 were chosen,this was done by PCR amplification of marker sequences using fluorescently labeled primers and subsequent analysis of the PCR products on ABI prism 377.Results Twenty alleles were observed in the population,with sizes ranging from 84 to 144 bp.There was no significant difference when compared with controls in the distribution of the genotypes(?2=21.428,P=0.208).This investigation indicated that the gene of CTLA4 was not susceptible gene for Graves disease.Conclusion This study suggests that CTLA4 gene may not be susceptible gene to Graves disease for the Chinese southern Han population.

Objective To study the mechanism of adaptive immunity against Rhizopus arrihizus (R. arrihizus) infections. Methods Bone marrow derived dendritic cells (BMDCs) were separated from C57BL/6 mice and Card9-/- mice and then were cultured in vitro. Resting spores and swollen spores of R. arrihizus were in vitro co-cultured with BMDCs with or without Syk inhibition. Secretion of cytokines ( IL-23, IL-1βand IL-12) was analyzed by ELISA after 24 hours of culture. Na?ve T cells derived from C57BL/6 mice were in vitro co-cultured with spore-stimulated BMDCs for four days. Levels of IL-17A and IFN-γ in supernatants of cell culture were analyzed by ELISA. Flow cytometry was performed to analyze T cell differ-entiation. Confocal microscopy was used to observe the images of stained β-glucan on the surface of resting and swollen spores. Swollen spores were co-cultured with Dectin-1, Dectin-2, TLR2 and mannose receptor ( MMR) , and the binding results were analyzed by flow cytometry. Results Swollen spores but resting spores could induce the maturation of BMDCs and promote the secretion of cytokines (IL-23, IL-1βand IL-12). Co-culturing T cells with swollen spore-stimulated BMDCs enhanced their differentiation to Th17 and Th1. In addition, swollen spores promoted the secretion of Th1-related cytokine ( IFN-γ) and Th17-related cytokine (IL-17A). Adding Syk inhibitor to Card9-/-BMDCs or wild type BMDCs significantly inhibited the secretion of cytokines and T cell differentiation, especially in the Card9-/- group. β-glucan was overserved on the surface of swollen spores, but not on resting spores. On the surface of swollen spores existed pathogen associated molecular patterns ( PAMPs) that could bind with Dectin-1 and TLR2. Conclusion Swollen spores of R. arrihizus could active BMDCs to secrete cytokines of IL-23, IL-1β and IL-12 and trigger T cell responses in vitro. The possible mechanism might be associated with β-glucan exposed on the surface of swollen spores that binds with Dectin-1. The responses between BMDCs and R. arrihizus are Syk-Card9-dependent.

OBJECTIVE To evaluate the efficacy and side effect during target controlled infusion of remifentanil and propofol in elderly patient undergoing nasal-endoscopy surgery.METHODS 60 patients of nasal-endoscopy surgery were devided into two groups based on age(non-elderly group age

OBJECTIVE:To establish the method to control the quality of Qilian suppository.METHODS:TLC was per?formed to identify Rhizoma coptidis and Radix Notoginseng.A reverse phase HPLC-ELSD method was adopted to determine the content of ginsenoside Rg 1 and ginsenoside Rb 1 in Radix Notoginseng.RESULTS:The study on the quality control showed that the characteristic of identification by TLC was distinct and highly specific.In this quantification method,the linear ranges of ginsenoside Rg 1 and Rb 1 were1.56?g～3.74?g(r=0.9993,RSD=1.6%)and1.44?g～3.46?g(r=0.9981,RSD=1.2%)respectively.The average recovery rate of ginsenoside Rg 1 was99.10%,and RSD=1.6%(n=3);the average recovery rate of ginsenoside Rb 1 was102.13%,and RSD=1.2%(n=3).CONCLUSION:The method is simple,sensitive,accurate and re?peatable.It can be used for the quality control of Qilian suppository.