The authors reported the results of anatomicat study of the scapular region (ninety-four sides) and the use of the parascapular composite flap in the repair of skin defects of the hand and foot.The parascapular flap is supplied by the circumflex scapular vessels. For exposure of the vascular pedicle an incision is made 7cm below the mid-point of the scapular spine, extending from the posterior axillary fold to the mid-point of the spine. Muscles are retracted and the circumflex scapular vessels are seen winding over the axillary border of the teres minor. Then the incision is extended from both ends of the above incision toward the inferior angle of the scapula to form an elliptical incision. The flap is raised by subfascial dissection from the mid-point of the spine towards the axilla, and ended by dividing the pedicle at the base of the circumflex scapular vssels. Since October 1982, the authors have applied flaps of such kind to repairing massive skin delects in the hand and the foot in 7 patients with success.

Objective To establish the method of content determination of Berberine in Zhenjing San. Method The analysis was carried out on C18 column (4.6 mm?250 mm, 5 ?m) by HPLC. The mobile please was acetonitrile-0.033 mol/L KH2PO4 (22∶78), the flow rate was 0.800 mL/min and detective wave length was 345 nm. Result The cabibration curves showed good linearity in the range of 12.012～600.600 ?g. The average recovery was 98.85% (RSD=1.09%). Conclusion The method is good sensitive, accurate and reliable.

The feasibility and mechanism study on intravenously injected bone marrow mesenchymal stem cells (MSCs) improving bladder function after spinal cord injury (SCI) were discussed by review the biological characteristics of MSCs, the changes and mechanism of the lower urinary tracts after SCI, and the study on the MSCs promoting recovery of bladder function.

Objective To investigate the value of radionuclide bone imaging in the diagnosis and localization of osteoporotic vertebral compressive fracture.Methods Percutaneous vertebroplasty was applied to 40 patients suffering from osteoporotic vertebral compressive fracture.Responsible vertebral bodys located according to the radionuclide bone imaging was involved as bone scan group,and responsible vertebral bodys located according to the physical examination,X-ray,CT and MRI as standard group.Then the Kappa test was carried out.Result In bone scan group,56 vertebrae were located by radionuclide bone imaging,and in standard group,58 vertebrae were located by X-ray,54 vertebrae by CT and 52 vertebrae by MRI.The Kappa value was 0.76,indicating essentially excellent agreement and statistical significance.Conclusion Radionuclide bone imaging was another good choice for the diagnosis of osteoporotic vertebral compressive fracture.Especially for cases with contraindication of MRI,it offers a reliable choice.

Objective To investigate the association between a missense mutation (T/G, Ser 1369 Ala) in exon 33 of sulfonylurea receptor 1(SUR1) gene in type 2 diabetes patients and the glucose-lowering effect of Gliclazide. Methods One hundred and four type 2 diabetes patients were selected and orally administered with Gliclazide for 56 days. Venous fasting plasma glucose levels (FPG), plasma glucose levels half an hour after taking 75 g glucose (OGH) and two hours (OGT) after taking Gliclazide were measured. Ser1369Ala polymorphism genotypes(TT, TG, GG) of SUR1 gene were determined by Taqman method. The glucose-lowering effect of Gliclazide was compared among different groups. If FPG was reduced more than 20%, we define it as effective. People whose blood sugar level was effectively reduced and not significantly reduced were compared to find whether their genotype groups were different.Results The change of FPG,OGH and OGT on day 1 and day 57 among TT,TG and GG genotype groups had no statistically significant difference. There was no statistically significant difference of the efficacy of Gliclazide in recessive model (TT+TG vs GG). The combination of TG and GG was more effective in reducing blood sugar level than TT. Conclusion Patients carrying one or two copies of the G allele were more sensitive to Gliclazide than patients carrying T allele.

Objective To evaluate the effects of two different short-term intensive therapies on ?-cell function in newly diagnosed type 2 diabetic patients.Methods Twenty newly diagnosed type 2 diabetic patients were divided into two groups of 10 patients each and randomly treated with glargine or glimepiride plus metformin for 4 weeks.The improvement of ?-cell function,FPG,PPG,HbA1c were measured before and after intensive therapy in each group.The difference in the improvement of ?-cell function,blood glucose and the frequency of hypoglycemia were compared between groups.Results After the treatment,FPG(12.25 vs.6.05 and 11.83 vs.6.35mmol/L),2hPG(18.67 vs.8.11 and 16.95 vs.8.73mmol/L),HbA1c(8.98% vs.7.38% and 9.17% vs.7.71%) were significantly decreased(all P

BACKGROUND:Percutaneous vertebroplasty is mainly used to treat senile osteoporotic compressive fractures,while for young patients,pedicle screw internal fixation is usually applied,but heavy trauma,nail broken,secondary surgery and other problems can be found occasionally.OBJECTIVE:To investigate the clinical efficacy of percutaneous vertebroplasty combined with granulated allogeneic bone grafting for thoracolumbar compressive fractures.METHODS:Twenty-one patients suffering thoracolumbar compressive fractures were recruited,and underwent percutaneous vertebroplasty combined with granulated allogeneic bone grafting.The Visual Analogue Scale scores,height ratio of fractured vertebrae and Cobb angle were detected before and after surgery.RESULTS AND CONCLUSION:(1) Compared with baseline,the Visual Analogue Scale scores were improved significantly at 1 day and 6 months postoperatively (P ＜ 0.05).(2) The height ratio of fractured vertebrae at 1 day and 6 months postoperatively was significantly higher than that before treatment (P ＜ 0.05).(3) The Cobb angle of fractured vertebrae at 1 day and 6 months postoperatively was significantly less than that before treatment (P ＜ 0.05).(4) The mean operation time was (52±12.3) minutes,and the mean blood loss was (11.5±1.5) mL.(5) These results suggest that percutaneous vertebroplasty combined with granulated allogeneic bone grafting can effectively restore the anterior fractured vertebral height,eliminate the cavity in the fractured vertebrae,renew the completeness and stabilization of the fractured vertebrae,which keep the spinal range of motion;therefore,it is a minimal invasive method in the treatment of thoracolumbar fractures.

The Experiments were performed on 63 urethane anesthetized, suxamethonium chloride paralyzed and artificially ventilated rats. The results were as follows: microinjection of L-Arg, NO precursor, into parabrachial nuclei (NPB) resulted in a marked decrease in the mean arterial pressure (MAP), but the heart rate had no significant change. Microinjection of N-nitro-L-arginine (L-NNA), NO synthase inhibitor, into NPB caused a significant increase in MAP. Preinjection of L-NNA and methylene blue into NPB separately blocked the depressor effect elicited by L-Arg. Preinjection of bicuculine, γ-aminobutyric acid (GABA) receptor antagonist, into NPB significantly reduced or abolished the depressor effect elicited by L-Arg. The above results indicated that: (1) NO has depressor effect in NPB by activating guanylate cyclase; (2) Endogenous NO in NPB has tonic depressor effect and takes part in maintenance of normal blood pressure level; (3) The depressor effect evoked by NO in NPB is at least partly mediated by local GABA receptor.

An endogenous element-label plus exogenous element-tag strategy was proposed for inductively coupled plasma mass spectrometry ( ICP-MS) to screen and discriminate a family of ultratrace but biological important biomolecules. The feasibility of this novel idea has been demonstrated when setting seleno ( SeCys) and Se-containing ( SeMet) proteins ( peptides) as an example. Se-label naturally occurring in the biomole-cules acts an identifier for picking them up out of large amount of various coexisting proteins ( peptides) , and CH3 Hg-tag that can bind to SeCys but not SeMet fulfills the task of discriminating seleno and Se-containing ones based on the Se and Hg signals on ICP-MS. After confirmed using SeCys and GPx1, the Se-Hg dual-element labeling strategy together with ICP-MS was applied to screen and discriminate seleno and Se-contai-ning proteins ( peptides) in the water-soluble extracts of Se-enriched yeast, and seven selenoproteins ( pep-tides) were detected with both 202 Hg and 82 Se signals out of fifteen Se-containing species using RPLC/ICP-MS, providing valuable information for further identification using a high-resolution structure-selective mass spectrometer. This endogenous element-label plus exogenous element-tag dual-element approach implies that ICP-MS is not only able to quantify targeted proteins ( peptides) but also helpful to recognize targeted proteins during a discovery-based proteomic study.