Objective To investigate the value of radionuclide bone imaging in the diagnosis and localization of osteoporotic vertebral compressive fracture.Methods Percutaneous vertebroplasty was applied to 40 patients suffering from osteoporotic vertebral compressive fracture.Responsible vertebral bodys located according to the radionuclide bone imaging was involved as bone scan group,and responsible vertebral bodys located according to the physical examination,X-ray,CT and MRI as standard group.Then the Kappa test was carried out.Result In bone scan group,56 vertebrae were located by radionuclide bone imaging,and in standard group,58 vertebrae were located by X-ray,54 vertebrae by CT and 52 vertebrae by MRI.The Kappa value was 0.76,indicating essentially excellent agreement and statistical significance.Conclusion Radionuclide bone imaging was another good choice for the diagnosis of osteoporotic vertebral compressive fracture.Especially for cases with contraindication of MRI,it offers a reliable choice.

BACKGROUND:Poly(L-lactic acid) (PLLA) scaffold is a kind of widely used biomaterial in tissue engineering. However, low hydrophilicity and lack of surface cell recognition site of PLLA hinder its further application. OBJECTIVE:To study the biocompatibility of multi-walled carbon nanotubes/PLLA (MWCNTs/PLLA) nanofiber scaffolds with mouse neural stem cells in vitro. METHODS:Mouse neural stem cells were isolated. Then we used electrospinning to fabricate PLLA nanofibers and modified them with multi-walled carbon nanotubes. We assessed their biocompatibility with passage 3 mouse neural stem cells in vitro. RESULTS AND CONCLUSION:Scanning electron microscope showed that the neural stem cells could survive on both scaffolds. No cytotoxic effects were detected on both scaffolds by Cell Counting Kit-8 detection. The adhesion and proliferation abilities of neural stem cells on the MWCNTs/PLLA scaffold were significantly greater than those on the PLLA scaffold. Neural stem cells were found grow well and have normal morphology on both scaffolds under scanning electron microscope and by Hoechst 33342 staining. Besides, immunofluorescence staining showed MWCNTs/PLLA could promote neural stem cells to differentiate into mature neurons and neurites grew along with the nanofiber scaffold. In conclusion, the MWCNTs/PLLA nanofiber scaffold has better properties than the PLLA for transplanted cells and provides a good growth carrier for neural stem cells to be induced to differentiate into neurons, which is expected to have a great potential of applications in nerve tissue engineering.

Maltol is a chemical derived from Chinese herb Panax Ginseng. The effect of maltol on hemolysis and hydroperoxidation of erythro-cytes induced by active oxygen radical is presented. Maltol could protect hemolysis induced by H2O2 in vitro. When erythrocyte membrane protein was exposed to butylhydroperoxide in vitro, membrane protein was polymerized, and membrane lipids were oxidized to hydroperoxide compound. These oxidative reactions could inhibited by maltol. The results showed that maltol might act as a scavenger of superoxide of hydroxy radical as demonstrated by biochemical method and ESR spin method。

ObjectiveTo study the possibility of differentiation of human umbilical cord mesenchymal stem cells(HUCMSCs) into human male germ cells,and to explore a new source of cells for the treatement of male infertility.MethodsHUCMSCs were transplanted into the seminiferous tubules of the testis of infertile mice by microinjection.The survival rate,migration and germ cell markers of HUCMSCs in the mice testis were detected via immunohistochemistry,immunofluorescence and confocal laser scanning. ResultsHUCMSCs can survive in the mice testis for at least 120 days,and they can migrate from the lumens to the basement membrane.Immunofluorescence showed that HUCMSCs can further differentiate in the mice testicular environment,and express the germ cell marker.ConclusionsHUCMSCs can survive,migrate and differentiate into early male germ cell-like cells in the infertility mice testis after transplantation.

BACKGROUND:Percutaneous vertebroplasty is mainly used to treat senile osteoporotic compressive fractures,while for young patients,pedicle screw internal fixation is usually applied,but heavy trauma,nail broken,secondary surgery and other problems can be found occasionally.OBJECTIVE:To investigate the clinical efficacy of percutaneous vertebroplasty combined with granulated allogeneic bone grafting for thoracolumbar compressive fractures.METHODS:Twenty-one patients suffering thoracolumbar compressive fractures were recruited,and underwent percutaneous vertebroplasty combined with granulated allogeneic bone grafting.The Visual Analogue Scale scores,height ratio of fractured vertebrae and Cobb angle were detected before and after surgery.RESULTS AND CONCLUSION:(1) Compared with baseline,the Visual Analogue Scale scores were improved significantly at 1 day and 6 months postoperatively (P ＜ 0.05).(2) The height ratio of fractured vertebrae at 1 day and 6 months postoperatively was significantly higher than that before treatment (P ＜ 0.05).(3) The Cobb angle of fractured vertebrae at 1 day and 6 months postoperatively was significantly less than that before treatment (P ＜ 0.05).(4) The mean operation time was (52±12.3) minutes,and the mean blood loss was (11.5±1.5) mL.(5) These results suggest that percutaneous vertebroplasty combined with granulated allogeneic bone grafting can effectively restore the anterior fractured vertebral height,eliminate the cavity in the fractured vertebrae,renew the completeness and stabilization of the fractured vertebrae,which keep the spinal range of motion;therefore,it is a minimal invasive method in the treatment of thoracolumbar fractures.

Objective To explore the value of diffusion weighted imagine(DWI) and magnetic resonance angiography(MRA) for the clinical significance in acute cerebral infarction in the early stage.Method The results of DWI and MRA and traditional CT and routine MRI in 30 patients with acute cerebral infarction were analyzed.Result Ultra-acute and acute cerebral infarction could be shown on DWI,which may not be shown on CT and T 2.WI.The focus that were shown on T 2.WI were more clearly shown on DWI.Focus and degree of intracranial vascular lesion could be detected rapidly.Conclusion DWI and MRA are sensitive for diagnosis in the early stage of acute cerebral infarction.Cerebral constitution and cerebral vessels can be shown by DWI and MRA,thus offering reliable imaging data for thrombolytic therapy.

Objective To compare the severity of sensorineural hearing impairment resulted from radiotherapy (RT) and radiochemotberapy (CRT) in patients with nasopharyngeal carcinoma (NPC). Methods Between March 2002 and May 2007, 100 initially diagnosed NPC patients in Shandong Tumor Hospital and Qi Lu Hospital were randomized to RT group and CRT group. All patients underwent intensity modulated radiation therapy. In CRT group, concurrent and adjuvant CDDP were administered (CDDP 25 mg/m2/d for 3 days to 4 cycles). Pure tone auditory threshold examination was performed 1 week ,6 months, 1 year and 2 years after the completion of radiotherapy. Statistical analyses were performed using Mann-Whit-ney U test,chi-square test and Fisber's exact probability test. Results The high-frequency threshold was significantly increased in CRT group comparing with RT group at 1- and 2-year after the treatment. In RT group, the hearing threshold was impaired immediately after the treatment, partially recovered within the first year but impaired again after 2 years. In CRT group, hearing threshold was impaired at the same time and kept getting worse until 1 year after radiotherapy, which could not be recovered. Conclusions Patients with NPC treated with radiotherapy and concurrent/adjuvant chemotherapy have more severe sensorineural hearing impairment comparing with those with radiotherapy alone, especially to the high frequency sound in the speech range. Inner ear tissue tolerance should be redefined for patients receiving radiochemotherapy.

AIM: To observe the therupeatic effects of Shemai Zhusheye vs vitamine C injection in treating dilated cardiomyopathy (DCM).　METHODS: Fifty-two patients with DCM were divided into 2 groups, 32 patients in treatment group (M 20, F 12; age 46 a± s 13 a), 20 patients in control group (M 11, F 9; age 47 a±14 a). Besides the patients in two groups were treated with conventional therapy, the patients in treatment group were given Shenmai Zhusheye 30 mL and 5% glucose injection 100 mL, iv, gtt, qd for 2 wk. The patients in control group were given vitamine C 5.0 and 5% glucose injection 100 mL, iv, gtt, qd for 2 wk. 　RESULTS: Treatment group total effective rate was 84%. Control group total effective rate was 65% (P>0.05).　CONCLUSION: Shenmai zhusheye has better effect in treating DCM. Symptom improving rate of Shenmai Zhusheye has better than vitamin C.

Objective To analyze the clinical features and factors affecting prognosis for intracerebral hemorrhage (ICH) during pregnancy and postpartum.Methods A study of ICH was performed on 61 women in Beijing Tiantan Hospital,Capital Medical University between January 1997 and December 2014,and all cases were diagnosed with cerebral hemorrhage or subarachnoid hemorrhage during pregnancy or six weeks after delivery with CT or MRI after exclusion of ICH due to craniocerebral trauma.The subjects were divided into surgery (n=26) and conservative treatment (n=35) groups according to different ways of treatment;pregnancy associated problems (n=11) and cerebrovascular diseases groups according to the aetiology of ICH;low (n=13) and high score group (n=48) according their Glasgow score at the first visit;and short clinical onset to diagnosis time (O-D time) group (≤ 24 h,n=33) and long O-D time (＞24 h) group (n=28).We compared the maternal clinical features and prognosis between different groups with t,Mann-Whitney U or Chi-square tests.A stratified logistic regression was used to assess the effect of factors affecting the prognosis.Results The average gestational age at the onset of ICH of the 61 cases was (28.8±8.3) weeks (6-40 weeks),the Glasgow score was (11.3±4.8),the median O-D time was 24.0 h,the modified Rankin scale (mRS) was 2.7,and 14 maternal deaths were reported (23.0％).Among the 61 women,three were terminated in early trimester,12 terminated in second trimester,and the rest 46 delivered in late term among which two fetal deaths,44 live births,and four neonatal deaths.Thus the perinatal infant death rate was 13.0％ (6/46).The difference of maternal clinical features and prognosis between the surgery and conservative treatment group was not significant (all P＞0.05).However,comparison between the cerebrovascular disease and pregnancy associated diseases group showed the latter had a lower Glasgow score and Apgar score [12.2(3.0) vs 7.5(12.0),(8.9±1.9) vs (7.2±2.6)],the higher mRS [2.4(2.0) vs 3.9(5.0)] and gestational age [(27.7±8.4) vs (34.9±4.1)],maternal mortality rate [14.0％ (7/50) vs 7/11] and perinatal death rate [5.4％ (2/37) vs 4/11] (t or x2=-3.09,-2.34,1.93,1.17,2.12 and 1.78,all P＜0.05).For women with low Glasgow score,the median O-D time was shorter than that of higer Glasgow score group (8.0 vs 48.0 h),the mRs and maternal mortality rate were higher 4.9(2.5) vs 3.1(2.0);9/13 vs 10.4％(5/48),t,U or x2=426.00,5.77 and 19.14,all P＜0.05].The short O-D time group showed lower Glasgow score and average Apgar score of the newborns than the long O-D time group [9.8(11.3) vs 13.2(2.0),(7.9±2.7) vs (9.2±0.9);t,U or x2=-2.91 and-2.23,both P＜0.05].The Glasgow scores was negatively associated with the mRs (OR=-0.26,95％CI:-0.16 to-0.05).In particular,O-D time (OR=0.03,95％CI:0.00-0.66) and pre-eclampsia (OR=0.33,95％CI:0.12-0.26) were both positively related to maternal mRs.However,the Glasgow scores,surgical treatment,O-D time and concomitant pre-eclampsia were irrelevant to the death ofperinatal infants (all P＞0.05).Conclusions The prognosis is poor in women with ICH during pregnancy or postnatal period whose Glasgow score was low or O-D time was long,or the ICH occurred due to pre-eclampsia.Antenatal care should be strengthened and early identification and diagnosis might improve the prognosis.

AIM: To study the protective effects of n on -mitogenic human fibroblast growth factor (nm-haFGF) on cardiomyocytes injured b y reactive oxygen free radicals. METHODS: The cardiomyocytes were isolated from neonatal SD mouse by trypsin digestion. The cardiomyocytes injury model was established by expos ing the cells to hydrogen peroxide (H 2O 2), and the injury status in cardiomy ocytes were evaluated by examining the cellular viability, measuring cell apopto sis and observing the change of cellular morphology. nm-haFGF was added to the c ulture medium, and the changes of cellular viability, superoxide dismutase (SOD) , malondialdehyde (MDA) and celluar apoptosis were observed. RESULTS: A dose-dependence relation between the concentration of H 2O 2 and the cardiomyocytes injury was observed. 10-80 ?g/L nm-haFGF dose -dependently increased cardiomyocyte viability and the general SOD activity, as well as decreased the content of MDA and the quantity of cardiomyocyte apoptosis . CONCLUSION: The higher the concentration of H 2O 2, the mor e serious the cardiomyocyte injury. nm-haFGF may have a good protective effects on cardiomyocytes treated with H 2O 2.