Objective:To induce experimental autoimmune uveoretinitis in Wistar for investigating the pathogenesis mechanism of uveitis.Methods:18 Wistar rats were immunized by different dosage of bovine soluble antigen(S Ag),and were detected the clinic changes dayly.The animals were anesthetized and killed when they had significant clinic EAU appearance or 3～4 weeks after immunized,the eyes were removed and followed by pathologic studies.Results:The incidence of EAU in the 3 different dosage S Ag immunized rats were 2/12 in 100 ?g S Ag group(6 rats,12 eyes),6/12 in 200 ?g S Ag group(6 rats,12 eyes) and 8/12 in 300 ?g S Ag group(6 rats,12 eyes) separately and the pathology scores were 0?16 76?11.02?17 56?9 96 separately in 3 group of rats.Conclusion:The middling sensitive Wistar rats can be used to induce EAU successly with middling purified S Ag.

Chronic Disease ; Graft vs Host Disease ; therapy ; Hematopoietic Stem Cell Transplantation ; Humans

Objective:To study if L-arginine cardioplegia can protect myocardium from ischemia/reperfusion injury. Method:Fourteen patients undergoing valve replacement were randomly divided into two groups:control group (crystal cardioplegia,C group),and test group(crystal cardioplegia+L-arginine,E group). Before aortic-clamping and after aortic unclamping,blood samples were taken to measure the concentrations of NO_2~-/NO_3~-, lactic acid (LA), malonylaldehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XOD). Immediately after aortic clamping and before aortic-unclamping, tissues were taken from the left atrial and examed with electron microscopy. Result: In C group NO_2~-/NO_3~- level decreased at aortic unclamping, 30 minutes later decreased significantly compared with that before aortic-clamping(P

OBJECTIVE To analyze the causes of unqualified medical appliance packs before sterilization and to find countermeasures in order to assure their quality,and to avoid emergence of hospital infection and medical tangles. METHODS Totally 320 packs were checked before sterilization from supply room and clinical departments from Jun 1,2004 to Mar 30,2005. RESULTS The rate of unqualified packs checked before sterilization was 14.12% including 460 medical apparatuses unqualified(21.70%). CONCLUSIONS To avoid the emergence of hospital infection and medical tangles,we should strengthen the work of supply room to realize the centralization of sterilization and supply,and make much of personnel′s professional training,normalize job′s process and quality criterion,and intensify professional and systematizing management of medical appliance purged and packed.

Objective:To research the autoimmune mechanism of prolactin(PRL) in uveitis by observing prolaictin′s effects on peripheral blood mononuclear cells(PBMC) proliferation and activation at different concentration.Methods:1.The serum prolactin levels of uveitis and control were checked by chemical spectrometer. 2.To research the effects of different concentration PRL on uveitis PBMC′s proliferation and activation under the soluble antigen(S-Ag) stimulation. PBMC separated from uveitis and control,were cultured with S-Ag and different concentration PRL,and then were assayed the percentage of CD3~+CD25~+ positive cell through double-stain immunofluorescence flow cytometry after 108 h. After cultured 96 h, ~3H-TdR was mixed and counts per minute were assayed after 12 h.Results:1.There was no significance between the uveitis and control refer to the serum PRL level(t=1.936,P=0.051). 2.When uveitis′ PBMC was positive to S-Ag, there had promote proliferation and activation of PBMC with PRL concentration at 12.5-1 000 ?g/L,with the media concentration at 200 ?g/L,while it became weakened at 1 000 ?g/L(activation t=2.736,P=0.012;proliferation t=2.547,P=0.036).When uveitis′ PBMC was negative to S-Ag,there had no action as before.Conclusion:1.There is no significance between the uveitis and control refer to the serum PRL level. 2.Media concentration PRL(200 ?g/L) and S-Ag have costimulation to promote PBMC proliferation and activation in uveitis,though high concentration PRL loses the function.

Objective To elucidates the effects of HPV18 E6 siRNA targeting at human papillomavirus(HPV)18 E6 gene on the proliferative activity of HeLa cells and chemotherapy sensitivity.Methods HPV18 E6 expression of HeLa cells was inhibited by siRNA interference,the change of P53 and P21 proteins expression level was measured by Western blot.MTT assay was used to detected proliferative activity and sensitivity to paclitaxel liposome of HeLa cells.Results After inhibition of E6 expression,P53 and P21 proteins increased and the growth of HeLa cells was decreased(P ＜0.01).The inhibition rate of HeLa was markedly increased after transfection of HPV18 E6 siRNA and paclitaxel liposome.Conclusion HPV18 E6 siRNA can effectively silence gene expression of E6 and inhibit proliferation of HeLa cells.HeLa cells are more sensitive to combine HPV18 E6 siRNA with paclitaxel liposome than that of control groups.

The standardization of data transfer plays an important role in precise radiotherapy,not only boosting the speed but also ensuring the treatment quality.In this article,a method for beam data generation and transfer is introduced based on DICOM RT Standard.Firstly,the realization procedure of this module is presented.Secondly,the realization means of two different IMRT methods including SMLC and DMLC are explained respectively.Finally,the system's validity is proved with instances.

Objective To investigate the therapeutic effect of different wavelength krypton lasers on diabetic retinopathy. Methods A total of 55 eyes (35 cases) with diabetic retinopathy underwent different wavelength krypton lasers photocoagulation treatment, according to the different manifestation of the affected eyes. The visual acuity, intraocular pressure,visual field,visual evoked potential were examined, and slit lamp, ophthalmoscopy, B ultrasonography, and fundus fluorescein angiography were performed preoperatively. The patients were followed up for at least 12 months after krypton laser treatment. Results The resulting effect on visual acuity after 12 months of photocoagulation in this series revealed that, 20 eyes (36.4%) improved, 34 eyes (61.8%) remained no change, and one eye (1.8%) decreased. Conclusions Different wavelength krypton lasers photocoagulation can be used in treatment of diabetic retinopathy and can improve the visual acuity at certain extent.

Objective To investigate the effect of bromocriptine on rats with experimental autoimmune uveoretinitis. Methods Tweenty four Wistar rats were immunized by bovine soluble antigen and randomly divided into treatment and control group. The rats in treatment group took bromocriptine orally with the dosage of 5 mg/(kg?d), which could inhibit prolactin (PRL) deliverance, while the rats in control group took glucose solution orally with the dosage of 50 g/(L?d). The clinical changes of all the rats and the delayed type hypersensitivity (DTH) response were detected. The rats were anesthetized and killed after immunized for 21 days, and the eyes were removed and examined histologically. Results The occurrence of EAU and histology scores of rats in treatment group were lower than the controls ( P 0.05). Conclusions Bromocriptine can generally inhibit PRL deliverance, and may also inhibit the occurrence of EAU in rats through neuroendocrine immune regulating network.

Objective This paper aims to find a best position of ephedrine hydrochloride nasal drops by comparing earache occurred in hyperbaric oxygen(HBO )therapy while using ephedrine hydrochloride nasal drops in two positions: laying in lateral position of 45°with head leaning back; laying down flat, so as to supply reference for prevention of earache. Methods We selected 300 cases of HBO patients who were firstly treated by HBO and randomly divided into the observation group and the control group with 150 cases in each group. All these patients were treated by large medical hyperbaric oxygen tank. On the first to third day, ephedrine hydrochloride nasal drops were put into the patients before they were sent into the oxygen tank. Patients in the observation group lay in lateral position of 45°with their head leaning back, and those in the control group were in horizontal position. We put three drops of 1% of ephedrine hydrochloride nasal drops respectively into their nasal cavity. When they came out of the oxygen tank, we asked them if they felt earache and checked their tympanic membrane so as to make a comparison. Results It was found that earache, rate of tympanic membrane injury and the extent of such injury in the observation group was obviously less than that in the control group, there was difference in statistics between these two groups. Conclusions Before HBO treatment, ephedrine hydrochloride nasal drops that put in lateral position of 45°with patients head leaning back can reduce the possibilities of earache occurred in HBO. It was also observed that such position was an ideal one which had anatomical characteristics of Eustachian tube and was safer than laying down flat, so such position is more practical.