Objective: To observe effect of salvia miltiorrhiza and ligustrazine injection on patients with acute episode of chronic pulmonary heart disease (CPHD) complicated pulmonary artery hypertension. Methods: A total of 40 CPHD patients with pulmonary artery hypertension were randomly divided into routine treatment group (n=20, received routine treatment) and observation group (n=20, received salvia miltiorrhiza and ligustrazine based on routine treatment). Another 20 healthy subjects were regard as healthy control group. The pulmonary artery pressure (PAP), partial pressure of oxygen in artery (PaO2) , left ventricular ejection fraction (LVEF) and levels of C-reactive protein (CRP) and endothelin-1 (ET-1) were examined and analyzed in all groups before and after treatment. Results: After treatment, compared with routine treatment group, there were significant increase in total effective rate (75% vs. 90%) and PaO2 [（83.87±14.53）mmHg vs.（92.95±13.54）mmHg], and significant decrease in mean PAP [mPAP, （55.43±9.65）mmHg vs.（45.52±8.89）mmHg], CRP [(6.37±2.12) mg/L vs. (3.29±0.84) mg/L] and ET-1 [(52.37±20.79) ng/L vs. (40.29±16.04) ng/L] in observation group (P<0.05~0.01). There was no significant difference in LVEF between the two groups after treatment (P>0.05). Conclusion: For patients with acute episode of chronic pulmonary heart disease complicated pulmonary artery hypertension salvia miltiorrhiza and ligustrazine injection can significantly improve clinical effects，right heart function, decrease mean pulmonary artery pressure, improve endothelial function and inhibit inflammatory reactions.

OBJECTIVE:To evaluate the stability of cefoxitin sodium for injection compatible with metronidazole injection fluid.METHODS:Cefoxitin sodium for injection was compatible with metronidazole injection fluid and the solution was stored ordinary temperature for4hours,the maximum absorption peak and absorbance of the solution were determined by UV,and the color of the solution was observed,and its pH value was determined.RESULTS:There were no significant changes in absorp-tion peak,absorbance,color and pH value of the compatible solution.CONCLUSION:Cefoxitin sodium for injection is com-patible with metronidazole injection fluid,and the compatible solution remains stable at ordinary temperature for4hours.

The Experiments were performed on 63 urethane anesthetized, suxamethonium chloride paralyzed and artificially ventilated rats. The results were as follows: microinjection of L-Arg, NO precursor, into parabrachial nuclei (NPB) resulted in a marked decrease in the mean arterial pressure (MAP), but the heart rate had no significant change. Microinjection of N-nitro-L-arginine (L-NNA), NO synthase inhibitor, into NPB caused a significant increase in MAP. Preinjection of L-NNA and methylene blue into NPB separately blocked the depressor effect elicited by L-Arg. Preinjection of bicuculine, γ-aminobutyric acid (GABA) receptor antagonist, into NPB significantly reduced or abolished the depressor effect elicited by L-Arg. The above results indicated that: (1) NO has depressor effect in NPB by activating guanylate cyclase; (2) Endogenous NO in NPB has tonic depressor effect and takes part in maintenance of normal blood pressure level; (3) The depressor effect evoked by NO in NPB is at least partly mediated by local GABA receptor.

Objective: To investigate the effects of Shen-Mai injection(SM) on perfusion and oxygenation of intestinaltract during repeffusion in shocked rabbits. Methods:Twenty-one rabbits were divided into control group (Ⅰ,n = 6), shock-repeffusion group ( Ⅱ, n = 9) and SM group ( Ⅲ, n = 6). Intestinal intramucosal pH (pHi) of the sigrnoid colon and portalvein blood gas was observed before shock, at 1 hour after shock, 1 hour and 2 hours of reperfusion. Results: pHi and portal vein pH in group Ⅱ were significantly lower than those in group Ⅰ (P < 0.01), but portal vein increased obviously during reperfusion. There was a good linear positive correlation between pHi and portal vein pH. Portal vein in group Ⅱ was greater than that in group Ⅰ and had anegative correlation with pHi. pHi and portal vein pH of group Ⅲ increased signifi- cantly compared with those of goup Ⅱ ( P < 0.05), while of group Ⅲ returned to the level of group Ⅰ . MAP and CO of group m were higher than those of group Ⅱ after 1 hour or 2 hours reperfusion (P<0.05).CO of group m remained at a high level during reperfusion. But SVR of group Ⅲ was lower than that of group Ⅱ during reperfusion. Conclusion: SM im-proves perfusion and oxygenation of intestinal tract during reperfusion.

Experiments were carried out in 31 urethane anesthetized, Suxamethonium chloride paralyzed and artificially ventilated rats. The results were as follows: microinjection of γ-amino butyric acid (GABA) into parabrachial nuclei (NPB) markedly decreased the mean arterial pressure (MAP) (P＜0. 001), while the microinjection of bicuculine (Bic) into NPB caused a significant increase in MAP (P＜0. 001). The depressor effect of GABA could be blocked by preinjection of Bic in NPB. The above agents microinjected into NPB had no significant effect on heart rate (P＞0. 05). The results indicated that: (1) GABA in NPB exerts tonic depressor effect; (2) Endogenous GABA in NPB exerts tonic depressor action and takes part in maintenance of normal blood pressure level.

Objective To investigate the effect of ulnar nerve with or without transposition during open reduction and internal fixation of distal humerus fractures.Methods A retrospective case control study was made on 74 cases receiving open reduction and internal fixation of distal humerus fractures between January 2008 and December 2015.There were 28 males and 46 females,with a mean age of 63.2 years (range,32-83 years).AO fracture classification was A2 type in seven cases,A3 type in four,C1 type in 14,C2 type in 28 and C3 type in 21.According to the intraoperative management of the ulnar nerve,the patients were divided into transposition group (n =43) and non-transposition group (n =31).Two groups were compared with respect to operation time,Mayo elbow performance score (MEPS),incidence of ulnar neuritis and postoperative complications.Results Follow-up lasted for 6-24 months (mean,11.7 months).Operation time differed significantly between transposition group [(110.9 ± 20.8) minutes] and non-transposition group [(95.6 ± 18.9) minutes] (P ＜ 0.05).MEPS in transposition group was excellent in 15 cases,good in 20,fair in six and poor in two.MEPS in non-transposition group was excellent in 10 cases,good in 15,fair in five and poor in one,with the same excellence rate of 81％ in comparison with transposition group (P ＞ 0.05).Incidence of ulnar neuritis showed no significant difference between transposition group (9％,4/43) and non-transposition group (7％,2/31) (P ＞0.05).Postoperatively,one case of superficial wound infection and two delayed healing were noted in transposition group;one case of superficial wound infection and one delayed healing were noted in non-transposition group.There was no significant difference in incidence of postoperative complications between groups (P ＞0.05).Conclusions Ulnar nerve with or without transposition can both achieve satisfactory clinical effect during open reduction and internal fixation of distal humerus fractures.But non-transposition group is associated with shorter operation time without increasing the incidence of ulnar neuritis.

We use PBL teaching mode in experimental teaching of clinical hematologic laboratory.By this method,the knowledge can be mastered by the students more efficiently and the analyzing ability was also improved.We hope that these experiences can be useful in our country's clinical laboratory teaching.

Objective To investigate prevalence of associated diseases in patients with gout as well as their diagnoses and treatment. Methods Two hundreds out-patients diagnosed with gout from April to October 2008 were investigated at Peking University People's Hospital, and information collected included their general characteristics, associated diseases, diagnoses and treatment, as well as blood lipid profiles, serum creatinine, uric acid, results of routine urine tests and glomerular filtration rate estimated by MDRD formula in the past three months. Results Among patients with gout, prevalence of associated hypertension, obesity, renal calculi, coronary heart disease, cerebral infarction and diabetes were 54.5% (109/200), 23.2% (42/181), 20.0% (40/200), 12.0% (24/200), 8.0% (16/200) and 7.0% (14/200), respectively, and 53.7% (101/188) of them associated with hypertriglyceridemia, 63.7% (114/179) with impaired renal function and 15.1% (27/179) with chronic kidney disease. In acute attack of gout, 124 (62.0%) of them were treated with non-steroidal anti-inflammatory drug (NASID), 72 (36.0%) with colchicines, 12 (6.0%) with corticosteroid and 30 (15.0%) with urate-lowering drugs, respectively, and during its intermittent period, 69. 8% (81/116) of them received urate-lowering therapy with indications generally accepted internationally, but serum level of uric acid could be maintained below 0.06 g/L in only 8.6% (10/116) of them. And 73.8% (48/65) of the patients with no therapy indications also were treated with urate-lowering drugs. Conclusions The most commonly associated diseases in gout patients are hypertension, hyperlipidemia and obesity, followed by renal calculi, chronic kidney disease and coronary heart disease, and so on. At present, oral NSAID is the first choice of drugs for its acute attack. Indications for urate-lowering therapy in this hospital usually are not consistent with those by generally international acceptance, with lower therapeutic effectiveness achieved.

Aim To explore effects of acidic fibroblast growth factor(aFGF)on the growth of renal tubular epithelial cells cultured in vitro. Methods Renal tubular epithelial cells in rats were gained through digestion by pamcreatin. aFGF was added into cultures of renal tubular epithelial cells, as experimental groups. The number of cells was counted and the shape of cells was observed at 12,24,48 and 72 h. Results Renal tubular epithelial cells were successfully obtained from kidneys. After treated for 72 h,the renal tubular epithelial cells showed different proliferation in experimental and control groups. There was no obvious difference at 12 h, but there was statistical difference between the two groups at 24 h,48h and 72 h(P