0.05) was observed. Bcl 2 expression was related with differentiation of GC(P0.05).Conclusion: p53,bcl 2,c erbB 2 may be involved in the development of GC.

Objective:To explore the influences of different sexes and ages on children static balance at the basis of quantitative comparison study of static postural balance of children aged 6-8(-year-odl).Method:A total of 499 children aged 6-8-year-old were recruited,which include 254 boys and 245 girls.Static postural balance test of these subjects was performed by using KY-1112 system for stabilometric analysis with subjects opening and closing eyes situations.Result:①There was a significant gender difference in static balance.At gender comparison,there was a significant difference on 3 indicators at the situations with eyes opening(P<0.05),and 3 indicators at the situations with eyes closing(P<0,01).②A significant difference Was shown in age comparison on 5 indicators(P<0.05).The mean of 5 indicators decreased with age growing.As compared with the value among Eve Area(with eyes opening and closing)and Track location Variance (with eyes opening).there Was a significant difference between children aged 8-year-old and 6-7-year-old(P<0.01),and no significant difference between 6-year-old and 7-year-old.There was a very significant difference on the LNG indicators at the situations with eyes opening and closing(P<0.01).③The value R indicator Was less than 1.Conclusion:To the children aged 6-8-year-old,there was significant gender difference in static balance,the balance function of girls was better than that of boys;there was significant age difference in static balance,with age growing the static balance rose also;relatively speaking,the gravity center shaking area was larger at forward and backward than that at leftward and rishtward.

Objective To discuss the safety and efficacy of gasless laparoscopy in gynecological surgery.Methods A total of 58 patients who received gasless laparoscopic surgery from October 2006 to December 2007 were enrolled in this study.Among the patients,31 underwent ovarian cystectomy,23 received myomectomy,3 laparoscopic-assisted transvaginal hysterectomy(LAVH),and 1 salpingectomy(because of ectopic pregnancy).One trocar was used in 13 cases(ovarian cystectomy in 11 and myomectomy in 2) with the assistance of hysteroscopic resectoscopy,two trocars were employed in 27 cases(ovarian cystectomy in 11 and myomectomy in 16),and three trocars in 18 cases(ovarian cystectomy in 9,myomectomy in 5,LAVH in 3,and salpingectomy in 1).The instruments employed in this study were purchased from Daoke Co.Results All of the 58 surgeries were successfully completed.In the patients received ovarian cystectomy,the mean intraoperative blood loss was(46.8?10.1) ml(ranged from 10 to 200 ml),and the mean operation time was(66.3?6.7) min(30-150).While in the patients underwent myomectomy,the mean intraoperative blood loss was(94.3?8.2) ml(10-400),and the mean operation time was(104.3?6.7) min(60-180).For the 3 cases of LAVH,intraoperative blood loss was 100,200,and 150 ml;and operation time was 255,240,and 135 min,respectively.The salpingectomy was finished within 30 min.None of the 58 patients developed complications during and after the operations.They were discharged from the hospital in(7.9?1.6) days,and were followed up for 3 to 6 months,during which no complications occurred.Conclusions Gasless laparoscopic surgery is safe,effective,and minimally invasive for gynecological diseases.The procedure can be used for eldly patients and those who cannot tolerate general anesthesia due to cardiovascular diseases.

Maltol is a chemical derived from Chinese herb Panax Ginseng. The effect of maltol on hemolysis and hydroperoxidation of erythro-cytes induced by active oxygen radical is presented. Maltol could protect hemolysis induced by H2O2 in vitro. When erythrocyte membrane protein was exposed to butylhydroperoxide in vitro, membrane protein was polymerized, and membrane lipids were oxidized to hydroperoxide compound. These oxidative reactions could inhibited by maltol. The results showed that maltol might act as a scavenger of superoxide of hydroxy radical as demonstrated by biochemical method and ESR spin method。

Aim To determine the effects of sodium tashinoneⅡA sulfonate(TSN) on monophasic action potential(MAP) and tachycardia-induced electrical remodeling of rabbit atria in vivo.Methods Twenty-four rabbits were equally divided into two groups randomly: control group and TSN group.Electrical catheters were localized in the right atrium through right internal jugular vein.Right atrial MAP was recorded by multiple channel recording. ERP of right atrial(AERP) was assessed by programmed electrical stimulation before pacing and from 0～8 hours after the onset of the pacing.Results The AERP_(200 ms) of control group was shortened from (105.9?3.8) ms to(114.7?7.2) ms and the rate-dependent of control group's atrium was lost through the pacing process compared with TSN group before pacing(P

Objective:To investigate the mechanism of the changes of the intestinal mucosal barrier in Severe acute pancreatitis.Methods:Twenty patients with Severe acute pancreatitis were admitted and 10 volunteers as the control group.The levels of serum tumor necrosis factor alpha(TNF_?)、nitric oxide(NO)、diamine oxidase(DAO) and the concentration of plasma endotoxin(ET) were measured.The ratio of lactulose to mannitol in urine was detected by HPLC with Pulsed Electrochemical Detection.Results:Compared with the control group,significantly increasing parameters could be seen in all the patients with Severe acute pancreatitis,including the ratio of lactulose to mannitol in urine (P

Objective To investigate the impact of percutaneous coronary interventional (PCI) on the inflammatory indices and postoperative vascular restenosis.Methods This study involved 90 patients undergoing PCI procedures for Coronary artery disease (CAD) compromising a single coronary artery.Fourty healthy individuals with normal findings by coronary angiography were selected as the control group.Before and after PCI or coronary angiography,plasma hs-CRP and IL-6 were measured in all the subjects by immunonephelometry and enzyme-linked immunosorbant assay (ELISA),respectively.Results (1) In the CAD patients,the plasma hs-CRP level was significantly elevated after PCI as compared with the preoperative level((18.69 ±5.14) mg/L vs (14.45 ± 4.32) mg/L,t =1.42,P ＜ 0.01),whereas in the control group,the hs-CRP level underwent no significant changes after coronary angiography((13.59 ±5.99) mg/L vs(12.46 ±5.35) mg/L,t =1.25,P ＞ 0.05).(2) PCI procedures also resulted in significant elevation of plasma IL-6 level in the CAD patients((1.87±0.45) pg/L vs (1.35 ±0.39) pg/L,t =1.33,P＜0.01),but in the control group,IL-6 showed no significant variation after coronary angiography ((1.32 ± 0.41) pg/L vs (1.21 ± 0.38)pg/L,t =1.16,P ＞ 0.05).We observed significant difference of hs-CRP and IL-6 levels between the CAD patient group and the control group (t =4.96,6.61 respectively,P ＜ 0.01).Conclusion Plasma hs-CRP and IL-6 are elevated in CAD patients following PCI procedures.But the roles of elevated hs-CRP and IL-6 in the vascular restenosis following the procedures need further investigation.

Objective To elucidates the effects of HPV18 E6 siRNA targeting at human papillomavirus(HPV)18 E6 gene on the proliferative activity of HeLa cells and chemotherapy sensitivity.Methods HPV18 E6 expression of HeLa cells was inhibited by siRNA interference,the change of P53 and P21 proteins expression level was measured by Western blot.MTT assay was used to detected proliferative activity and sensitivity to paclitaxel liposome of HeLa cells.Results After inhibition of E6 expression,P53 and P21 proteins increased and the growth of HeLa cells was decreased(P ＜0.01).The inhibition rate of HeLa was markedly increased after transfection of HPV18 E6 siRNA and paclitaxel liposome.Conclusion HPV18 E6 siRNA can effectively silence gene expression of E6 and inhibit proliferation of HeLa cells.HeLa cells are more sensitive to combine HPV18 E6 siRNA with paclitaxel liposome than that of control groups.

Objective To explore the feasibility of laparoscopically intracorporeal delta-shaped anastomosis in distal gastrectomy.Methods 82 consecutive patients with distal gastric cancer received laparoscopic distal gastrectomy, including intracorporeal delta-shaped anastomosis in 39 cases, and handassisted Billroth-Ⅰ anastomosis in 43 cases.The operative time, blood loss, anastomosis time were compared between the two groups.Results There were no differences between the two groups with regard to the operative time [(215 ± 24) min vs.(207 ± 13) min, t =0.406, P ＞ 0.05] and blood loss [(56 ±21)ml vs.(65 ± 26)ml, t =1.441, P ＞ 0.05], although the intracorporeal Delta-shaped anastomosis was more time-consuming than the extracorporeal Billroth-Ⅰ anastomosis [(17 ± 8) min vs.(12 ± 3) min, t =2.529, P ＜ 0.05].Conclusions Demanding expertise and some skills, totally laparoscopic distal gastrectomy with intracorporeal delta-shaped anastomosis is safe and feasible.

Objective: To investigate the effect of survivin and bcl-2 antisense oligodeoxynucleotides ( AsODN) combined transfection on the human gallbladder carcinoma cell line GBC-SD in vitro. Methods: Survivin and Bcl-2 protein expressions were detected by immunohistochemical method; Cultured cells were divided into 4 groups: Nomal control group, survivin antisense observed group, bcl-2 antisense observed group and combined group. After transfected for 24 h, the expression of survivin mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR). Cell morphological changes were observed under electron microscopy. Apoptosis index (AI) was examined by flow cytometry; Inhibitory rate (IR) was determined by the colorimetri MTT cell viability and proliferation assay. Results: Survivin and Bcl-2 protein were highly expressed in gall bladder carcinoma cells; The expression of survivin mRNA was decreased 47. 8%. Abnormal morphological changes of cells were observed in the three AsODN transfection groups; The AI in survivin antisense observed group,bcl-2 antisense observed group,and combined group was 11.38%?3.91% , 9.26%?4.15% , 28.45%?6.34% respectively and significantly higher than the nomal control group (P