Comparison between the DNA fingerprinting and the serological method used for paternity test is presented. 32 cases of disputed paternity were tested using DNA fingererints with MYO minisatellite DNA probe and blood typing. The results of DNA fingerprinting test correlated with those of blood typing. 8 alleged fathers were excluded while 24 were confirmed. The relationship between the results from two methods was discussed.

Aim To investigate the anti-tumor effect of nitidine chloride(NC)on human HepG2 hepatocellular transplanted tumor in nude mice and its effect on topoisomerase.Methods The subcutaneous transplantable tumor model of human liver cancer in nude mice was established and the anti-tumor effect of NC was calculated.The effects of NC on TopoⅠ/Ⅱ mediated-pBR322 DNA relaxation were measured by using agarose gel electrophoresis.Results NC inhibited significantly the growth of hepatoma,The inhibitory rate at the dose of 2.5,5,10 mg·kg~(-1) was 12.06%,35.63% and 60.91% respectively.At the concentration of 6.25 μmol·L~(-1),NC completely inhibited the pBR322 DNA cleavage mediated by TopoⅠ;at the concentration of 25 μmol·L~(-1),NC completely inhibited the pBR322 DNA cleavage mediated by Topo Ⅱ.Conclusion Nitidine Chloride can inhibit hepatic carcinoma growth in nude mice,The anti-tumor mechanism is probably related to the inhibitory effect on Topo.

Epithelial-mesenchymal transition (EMT) is that cells undergo a switch from epithelial phenotype to mesenchymal phenotype.Recent researches show that EMT can affect the occurrence and development of gastric cancer through a variety of mechanisms and promote the migration and invasion of tumor cells.The process of EMT involves a number of signal transduction pathways,and is related to microRNAs,Helicobacter pylori,transcription factors and so on.EMT is expected to be a novel therapeutic target of gastric cancer.

0.05);the cost-effectiveness ratios were 768.31,812.14 and 457.98,respectively.CONCLUSION:Scheme C was proved to be the best therapeutic regime.

OBJECTIVE:To observe the therapeutic effect of Breviscapine injection in treating migraine. METHODS: 81 patients with migraine were randomly divided into two groups: 42 in treatment group were treated with Breviscapine injection 20 mL added to 5% Glucose injection 300 mL iv gtt q.d, and 39 in control group with Flunarizine 10 mg po quaque nocte. The treatment lasted for 2 courses (1 course of treatment lasted 14 days) with an interval of 15 days between the two courses. The patients were followed for more than 6 months. The curative effects between the 2 groups were compared, and the variations in hemorheological indexes of the 2 groups before and after treatment were observed. RESULTS:The efficacy in the treatment was signficantly higher than in the control gorup(P

[Objective]To evaluate the safety and efficacy of the surgery,which was done by a video-assisted thoracoscope to release the anteriorroute part of spine and done by posterior instrumented fusion with severe Adolescent Idiopathic Scoliosis(AIS).Clinic date:There were 10 cases of AIS(King Ⅱ 6,King Ⅲ 3,King Ⅳ 1),in which there were 6 males and 4 females.The average age was 17.2 years(range 14~21).The average preoperative primary curve in the coronal plane was 91.3?(ranged 67?~125?)and the average pliability of thoracic spine was 28.3%(ranged 19.3%~36.5%).[Method]With general anesthesia(onelung ventilation or two-lung ventilation),the disc complexes and part of the anterior longitudinal ligament of 5~6 disc near apex vertebra were excised under endoscopic visualization.In the same operation,the posterior instrumented fusion was done.[Result]The anterior releases under endoscopic visualization needed 120~150 min with average bleeding 157ml(ranged 100~200 ml).The thoracic draining was 100~150 ml After the posterior route surgery,the average coronal Cobbs angle was 47?(ranged 26?~75?)and the rate of rectification was 51.5%.The patients had good fusion and outlook without other complications.[Conclusion]This way,which is done by a videoassisted thoracoscope to release the anterior part of spine and done by posterior instrumented fusion has a good result about safety and efficacy.

Objective:To induce experimental autoimmune uveoretinitis in Wistar for investigating the pathogenesis mechanism of uveitis.Methods:18 Wistar rats were immunized by different dosage of bovine soluble antigen(S Ag),and were detected the clinic changes dayly.The animals were anesthetized and killed when they had significant clinic EAU appearance or 3～4 weeks after immunized,the eyes were removed and followed by pathologic studies.Results:The incidence of EAU in the 3 different dosage S Ag immunized rats were 2/12 in 100 ?g S Ag group(6 rats,12 eyes),6/12 in 200 ?g S Ag group(6 rats,12 eyes) and 8/12 in 300 ?g S Ag group(6 rats,12 eyes) separately and the pathology scores were 0?16 76?11.02?17 56?9 96 separately in 3 group of rats.Conclusion:The middling sensitive Wistar rats can be used to induce EAU successly with middling purified S Ag.

AIM:To study the direct reprogramming method of mouse embryonic fibroblasts (MEFs) conver-ted into induced neural stem cells (iNSCs).METHODS:Sox2-infected MEFs were cultured in NSCs culture medium for 10 d.Subsequently , repeated suspension and adherent culture were performed for 3 times for the purification of iNSCs .The iNSCs were cultured in suspension medium .Real-time PCR was used to detect the expression of neural stem cell marker genes and pluripotent marker gene .In vivo, iNSCs were microinjected into the mouse cerebral cortex .Immunofluorescence was performed to detect the expression of neural stem cell , neuron, oligodendrocyte and astrocyte markers in vitro and vivo. RESULTS:A variety of neural stem cell marker gene expression was significantly increased in iNSCs detected by real -time PCR.Immunofluorescence confirmed that iNSCs expressed nestin and differentiated into neurons , oligodendrocytes and as-trocytes in vitro and vivo.CONCLUSION:Sox2 is sufficient to trigger the direct reprogramming from MEFs to iNSCs .iN-SCs have the ability of self-renew and 3 differentiation potentials in vivo and vitro.iNSCs are the suitable seed cells of SCI .

Objective To investigate the changes of serum cytokines in children with mycoplasma pneumoniae pneumonia (MPP).Methods A total of 239 children with MPP hospitalized in our department from Jan 2008 to Dec 2009 served as MPP group,which were redivided into mild MPP group (n =152) and severe MPP group ( n =87 ).Two hundred and sixty-three acute bronchopneumonia children without MPP infection served as non-MPP group.Fifty cases who would undergo hernia and phimosis elective surgery in pediatric surgery department served as control group.All children with pneumonia were detected mycoplasma pneumoniae antibodies and cytokines (TNF-α,IFN-γ,IL-1β,IL-4,IL-6) of serum in the first day of hospitalization and recovery period.Children in control group were detected the level of cytokines only once.Results Serum levels of TNF-α,IL-1β,IL-6 in MPP group and non-MPP group were higher than those in　control group ( P ＜ 0.01 ).No significant differences of IFN-γ and IL-4 levels were found among three groups ( P ＞ 0.05 ).Compared with non-MPP group,the levels of IFN-γ,IL-1β and IFN-γ/IL-4 were higher in MPP group ( P ＜ 0.01 ).The levels of TNF-α,IL- 1β,IL-6,and IFN-γ/IL-4 were significantly decreased in　the recovery period of MPP (P ＜0.001,P ＜ 0.05 ).The levels of TNF-α,IL-1β and IFN-γ/IL-4 in severe　MPP children were higher than those in mild MPP children ( P ＜ 0.01 ).Conclusion The immunologic　function of MPP children is unbalanced.The serum levels of TNF-α,IL-1β,IL-6,and IFN-γ/IL-4 are correlated with severity of MPP,which help to evaluate the state of MPP.

Experiments were carried out in 31 urethane anesthetized, Suxamethonium chloride paralyzed and artificially ventilated rats. The results were as follows: microinjection of γ-amino butyric acid (GABA) into parabrachial nuclei (NPB) markedly decreased the mean arterial pressure (MAP) (P＜0. 001), while the microinjection of bicuculine (Bic) into NPB caused a significant increase in MAP (P＜0. 001). The depressor effect of GABA could be blocked by preinjection of Bic in NPB. The above agents microinjected into NPB had no significant effect on heart rate (P＞0. 05). The results indicated that: (1) GABA in NPB exerts tonic depressor effect; (2) Endogenous GABA in NPB exerts tonic depressor action and takes part in maintenance of normal blood pressure level.