Objective To immunoscreen one protein antigen gene from a cDNA library of cysticercus cellulosae. Methods The cDNA library of cysticercus cellulosae was immunoscreened by using the serum of patient with cerebral cysticercosis. After the insert of positive clone was amplified by PCR and subcloned into pUC18, the nucleotide sequence of the cDNA was determined by the dideoxynucleotide chain termination method using a Taq DyeDeoxy Terminator Cycle Sequencing Kit. The homological search of the nucleotide sequence was done by using BLASTN in NCBI. Results A cDNA clone (named cY1) with a length of 546 bp was isolated. The clone contained one open reading frame composed of 474 bp encoding 158 amino acids. The nucleotide sequence of cY1 showed 99% homology with one immunogenic protein gene named NC-9 of cysticercus cellulosae. Conclusion A gene encoding protein antigen of cysticercus cellulosae is cloned.

Acute lung injury ( ALI) is a type of pulmonary excessive inflammation with high morbidity and mortality.It can be induced by multiple causes.There are currently no successful therapeutic or preventive measures because the patho-genesis of ALI has not been completely clarified.Many studies have shown that the activation of complement 5a (C5a) and its receptors is necessary in the process of ALI.Drug development targeting on C5a and its receptors may bring new hope to the treatment of ALI.In this article, the experimental evidence and possible mechanisms are summarized to reveal the rela-tionship between C5a and ALI.

Objective To analyze the outcome of reoperation in drug resistant epilepsy after failed surgery or recurrence in children,and to identify the predictors of the outcome of reoperation and inspiration of epilepsy resection surgery.Methods Analysis was done on data of 4 patients with drug resistant epilepsy diagnosed in Department of Neurosurgery Epileptic Center,Fuzhou General Hospital of Nanjing Military Command from Sep.2011 to Sep.2012.Clinical manifestation,preoperative evaluation,postoperative follow-up of the children were analyzed.Results After first epilepsy resection surgery,3 patients had little decrease in seizure rate(Engel class Ⅳ),the other one had seizure freedom (Engel class Ⅰ),but got recurrence after 2 years.Four cases were all underwent extended resection reoperation du-ring the study period.Types resection included temporoparietooccipital (3/4 cases) and frontal (1/4 cases),mean follow-up 11.25 months.One of the 4 patients had seizure freedom (Engel class Ⅰ),and 3 cases of the 4 patients had seizure reduction (Engel class Ⅱ) at last follow-up,in addition,none of them had significant or unacceptable complication.Conclusions Patients with recurrent drug resistant epilepsy after an initial resection surgery can regain seizure freedom outcome when they underwent repeat resection surgery after comprehensive reevaluation.Predictors with likelihood of seizure freedom after reoperation are:(1) retained medial temporal structures or relevant magnetic resonance imaging structural lesion;(2) original surgery suboptimal; (3) preceding evaluation suggests solitary identified focus;(4) semiology relate to preoperative seizures.

OBJECTIVE To inquire into the risk factors of uremia patients with fungus infection and search for the effective methods to prevent and control it.METHODS All the cases of the 82 uremia patients with fungus infection in the clinic of our hospital were analyzed.RESULTS The sites of the fungus infection were intestines and stomach,blood vessel,respiratory tract,oral cavity,reproductive tract,skin soft tissue and urinary tract.The common fungus infection was Candida albicans,about 60.61 per cent.The risk factors of uremia patients with fungus infection were the old age,several underlying diseases,hypoimmunity,not using medicine properly during the treatment and invasive operation.CONCLUSIONS The effective methods to prevent and control fungus infection are as the follows: to improve the staff′s awareness of the danger of fungus infection,be strict to the rules of hospital isolation and sterilization,and be try to reduce invasive operation.We should strengthen the cleaning of the wards and the crew's hands,strengthen the monitoring of quality and pay more attention to the primary treatment.We should use the medicine properly,especially the use of antibiotics,immune suppressants and hormones,and pay attention to result of culture from the microbiological laboratory.We should cure the underlying diseases actively and improve the suffers′ immunity function.

Objective To assess pathological features of muscles as well as microvascular changes between muscle fibers in patients with dermatomyositis (DM),and to analyze pathological differences in muscles between DM and polymyositis (PM).Methods Specimens were obtained from involved muscles of 16 patients with DM,5 patients with PM,and from normal muscles of 9 patients with bone trauma (controls).Routine histopathological examination and immunohistochemical staining for CD34 and CD61 were conducted.Results Of the 16 patients with DM,6 (37.5％) had perifascicular atrophy,3 ( 18.8 ％) had an obvious inflammatory cell infiltration around microvessels between muscle fibers.Perifascicular atrophy was absent in muscle specimens from patients with PM or bone trauma.The number of CD34-positive microvessels between muscle fibers was reduced in patients with DM,but normal in those with PM.CD61 was positive in perifascicular area of 10 patients (5 moderately positive and 5 weakly positive) with DM,with an expression rate of 62.5％,however,only 1 case of PM was weakly positive for CD61,and all the controls were negative.Conclusions There is a decrease in the number of microvessels but an enhancement of neovascularization between muscle fibers in involved muscles of patients with DM,which may serve as a pathological marker to distinguish DM from PM.

Objective To examine the role of c-Jun NH2-terminal kinase(JNK) 1 in cytolytic T lymphocyte (CTL) responses against virus infection.Methods Wild-type (WT) and JNK1-knockout (JNK1-/-) mice were infected with ectromelia virus(ECTV) through hind footpads.Survival and virus titers in the target organs (liver and spleen) were analyzed.Effector T cells in the spleen and popliteal lymph nodes were determined on day 3 and 7 post-infection.Proliferation and INF-γ production of CTL were also detected.Results JNK1 deficiency caused an increased susceptibility to ECTV infection in mice,indicated by higher case fatality and viral burden in target organs.The decrease in CTL response correlated with a defect in CTL proliferation and INF-γproduction.Conclusion The data suggest that JNK1 is involved in expansion of activated CTL during ECTV infection,and plays an important antiviral role in regulating the proliferation and effector function of CTL.

Objective To explore the mechanism underlying the effects of gypenosides (GP) against photodamage. Methods Eighty BALB/c mice were equally divided into 8 groups, i.e., blank control group (untreated), UVB model group (irradiated with UVB), GP I group (irradiated with UVB before topical application of GP), GPⅡ group (irradiated with UVB followed by topical application of GP), VitE I group (irradiated with UVB after topical application of Vitamine E cream), VitE Ⅱ group (irradiated with UVB followed by topical application of Vitamine E cream), Vehicle group Ⅰ (irradiated with UVB after application of the drug vehicle),and Vehicle group Ⅱ (irradiated with UVB before application of the drug vehicle). UVB irradiation was performed once every other day for 14 days. Mice were sacrificed after the last irradiation and skin specimens were obtained from the irradiated sites, and the levels of p53 and p21 protein were measured by Western blot in the specimens. Results The expression level of p53 protein was significantly lower in the blank control group than in the UVB model group (0.11 ± 0.08 vs. 0.22 ± 0.12) and GP Ⅰ group (0.44 ± 0.23, P < 0.01),in the blank control group and UVB model group than in the GP Ⅱ group (0.48 ± 0.24, P < 0.01, 0.05). VitE Ⅰ group (0.49 ± 0.29) and VitE II group (0.50 ± 0.27) were similar to the GP groups in the expression of p53 protein. No statistical difference was observed in the expression of p21 protein between the eight groups. Conclusion The upregulation of p53 protein expression in epidermal cells may be related to the mechanisms underlying the protective effect of 1.5% GP cream against photodamage.

Objective To observe the effects of gypenosides (GP) on nuclear factor κB (NF-κB) and p38 mitogen activated protein kinase (p38MAPK) signaling pathways in photodamaged skin of mice,and to explore the mechanisms underlying the protective effects of GP against photodamage.Methods Eighty Balb/C female mice were randomly divided into 8 groups: blank control group receiving no treatment,ultraviolet B (UVB) model group receiving UVB irradiation for 60 seconds,GP group Ⅰ receiving topical GP treatment followed by UVB irradiation,GP group Ⅱ receiving UVB irradiation followed by topical GP treatment,VitE group Ⅰ receiving topical VitE treatment followed by UVB irradiation,VitE group Ⅱ receiving UVB irradiation followed by topical VitE treatment,matrix group Ⅰ receiving topical matrix treatment followed by UVB irradiation,matrix group Ⅱ receiving UVB irradiation followed by topical matrix treatment.UVB irradiation lasted 60 seconds at one time,and was given once every other day for 7 times to establish a skin model of photodamage.The interval between irradiation and topical treatment was 30 minutes in all the groups except the control group and UVB model group.After the last treatment,mice were sacrificed.Western blot was performed to measure the protein expressions of inhibitor of NF-κB(IκB),inhibitor of NF-κB kinase (IKK),p38MAPK as well as phosphorylated p38MAPK (pp38) in skin tissue from the mice.Results No expressions of IκB or IKK were observed in the blank control group.The expression level of IκB was 0.40 ± 0.07 in UVB model group,significantly lower than that in GP group Ⅰ (1.63 ± 0.85,P ＜ 0.05) and GP group Ⅱ (0.90 ± 0.40,P ＜ 0.05),whereas the level of IKK protein was higher in UVB model group than in the GP group Ⅰ and Ⅱ (2.01 ± 1.75vs.0.23 ± 0.12 and 0.45 ± 0.29,both P ＜ 0.05).No significant difference was observed in the expression of IκB or IKK proteins between the GP group Ⅰ,Ⅱ,VitE group Ⅰ and Ⅱ or in the expression of p38MAPK between the 8 groups.The phosphorylated p38MAPK expression in UVB model group was significantly higher than that in GP group Ⅰ and Ⅱ (0.835 ± 0.049 vs.0.425 ± 0.054 and 0.571 ± 0.090,both P＜ 0.05),but similar to that in VitE groups.Conclusions UVB can activate NF-κB and phosphorylated p38MAPK signaling pathways; GP 1.5％ cream can inhibit UVB-induced activation of NF-κB and p38MAPK pathways,which may be one of the mechanisms underlying its protective effects against inflammation and photodamage.

Objective To investigate the most appropriate low radiation dose in multi-slice CT (MSCT) scans for orbital trauma patients. Methods Thirty trauma patients with suspected orbital fractures who underwent helical CT scans with a 64-MSCT using regular dose were selected. Noise was artificially introduced to the axial images using an image space noise addition tool to simulate 6 sets of lower dose scans with tube current of 30, 70, 100, 140, 170 and 200 mA, respectively. The lowest tube current with adequate image quality for confident diagnosis was determined based on the evaluation of the overall image quality and fracture detection on images at different dose levels. The determined lowest tube current was then validated using clinical scans. Radiation dose related parameters CTDIvol, DLP, ED were also recorded.Image quality was evaluated according to its low-density resolution, noise and structure clarity and characterized into 5-grades of excellent, good, fair, worse and worst Rank sum test and X2 test were wsed for statistics. Results In 30 trauma patients with regular dose of 300 mA, there were 30 cases of orbital fracture, 19 cases of intraorbital emphysema, 12 cases of ocular muscle injury and 1 case of intraorbital foreign body. These changes could still be clearly observed and correctly diagnosed when the tube current was reduced to as low as 70 mA. However, the overall image quality was mostly fair. At the simulated dose of 100 mA, the majority of images were characterized as excellent or good, and there was no statistical difference compared with that of regular dose scans (P > 0.05 ) . In the clinical evaluation for 20 orbital trauma patients with the reduced tube current of 100 mA, the majority of images were judged to be excellent (9 cases) or good(17 cases) and fair (4 cases). The radiation dose(0. 29 mSv) was reduced by 70% compared with that of regular tube current of 300 mA(0. 86 mSv). Conclusion The tube current of MSCT may be used as low as 100 mA in orbital trauma patients to reduce radiation dose and keep the image quality diagnosable.

Objective: To evaluate dissolution properties of 2 kinds of ambroxol hydrochloride tablets.Methods: A HPLC method was used.The column was Hypersil C 18 (10 ?m,150 mm?4.6 mm).The mobile phase was methnol water tetrahydrofuran triethylamine (71∶29∶2.5∶0.25).The wavelength of detector was 242 nm.Data were analyzed by Weibull's distribution model.Results: Both tablets dissolved rapidly and their dissolution rate reached 85% in the first 8 min.But there was significant statistic differences between the 2 tablets as for Weibull's parameters.Conclusion: Although the dissolution rate of 2 tablets is similar,there are still some differences between their dissolution parameters.The dissolution of the imported tablets is more rapid than the home made ones.