Objective To investigate the clinical efficacy of acupuncture plus Chinese herbal medicine in treating allergic rhinitis. Methods One hundred and thirty-five patients with allergic rhinitis were randomly allocated to combination, acupuncture and Chinese herbal medicine groups, 45 cases each. The combination group received treatment with acupuncture plus Chinese herbal medicine; the acupuncture group, treatment with acupuncture alone; the Chinese herbal medicine group, treatment with Chinese herbal medicine alone. IgE, IL-4, IL-5 and TNF-αlevels and clinical symptom and sign total score were observed in the three groups before and after treatment. The clinical therapeutic effects were compared between the three groups. Results There were statistically significant pre-/post-treatment differences in IL-4, IL-5, TNF-αand IgE levels in the three groups (P<0.05). There were statistically significant post-treatment differences in IL-4, IL-5 and IgE levels between the combination group and the Chinese herbal medicine or acupuncture group (P<0.05). There was a statistically significant pre-/post-treatment difference in clinical symptom and sign total score in the three groups (P<0.05). There was a statistically significant post-treatment difference in clinical symptom and sign total score between the combination group and the Chinese herbal medicine or acupuncture group (P<0.05). The total efficacy rate was 91.1%in the combination group, 71.1%in the Chinese herbal medicine group and 66.7%in the acupuncture group. There was a statistically significant difference in the total efficacy rate between the combination group and the Chinese herbal medicine or acupuncture group (P<0.05). Conclusions Acupuncture plus Chinese herbal medicine has a better therapeutic effect on allergic rhinitis. It can effectively relieve the clinical symptoms and signs and reduce IgE, IL-4 and IL-5 levels in the patients.

Objective To study on extraction and characteristics of ?-glucosidase inhibitor from Chlorella vulgaris.Methods The extracts were prepared by drying,degreasing and silica gel columniation washing with different organic solvents,and its pure degree and components were identified.The activities of the inhibitor and its effects on ?-glucosidase under different conditions were studied.Results The results showed that ?-glucosidase inhibitor from Chlorella vilgaris may be a kind of substance of hydroxybenzene.Conclusion The inhibitor showed potent inhibitive effection on ?-glucosidase,and belong to a mechanism of noncompetitive inhibition

Objective Macrophages are important innate immune cells,and are also the main target cells of Mycobacterium tuberculosis.Macrophages express many kinds of Toll like receptors.TLR2 is a structural component of macrophages in the identification of Mycobacterium tuberculosis.Activation of TLR2 signaling pathway can activate cell signal transduction,induce specific gene expression and secretion of cytokines.Immune function decline in old people and the function of macrophages was also changed.In this article,The role of immunity mediated by TLR2 in macrophages against tuberculosis in the elderly is reviewed.

AIM: To optimize the parameters of processing for Arctium Lappa.METHODS: The contents of arctiin and arctigenin in Arctium Lappa were determined with HPLC,and the total yields of them were chosen as in-dex.The orthogonal test was adopted to acquire the optimum technology for processing Arctium Lappa.RESULTS: The study showed that the best processing parameters for Arctium Lappa consisted of 300 ℃ of the drying tempera-ture and 4-5 minutes of processing time.CONCLUSION: The processing method is reasonable,stable and reliable,and can be used in commercial production.

AIM:To establish an HPLC fingerprint spectrum of Red Peony Root ( Radix paeonia rubra). METHODS:Ten batches of samples were extracted by 50% ethanol and extract liquor was evaporated to dryness,resolved with methanol. The HPLC analysis was performed on a Diamonsil C18 column with the eluting system of gradient acetonitrile and water. MS was applied to the determination of possible chemical structure. RESULTS:Nine main characteristic peaks were selected from the fingerprint spectrum of 10 batches of samples. Five constituents of which were identified as gallic acid,peoniflorin albiflorin,benzoylpaeoniflorin and paeonol based on the MS spectra. CONCLUSION:The method is valuable for quality conthol of Red Peony Root.

Objective To investigate correlation study of serum hs-CRP,UA level and the correlation of dis-ease in elderly acute myocardial infarction patients , and provide a reference basis for clinical judgment for patients with illness development and prognosis prediction .Methods 40 aged patients with acute myocardial infarction pa-tients were selected as the research object ,and protionally random according to time admission the same period ran-domly selected were 40 cases of elderly patients with unstable angina pectoris ,40 cases of elderly patients with stable angina,40 cases of clinic medical normal healthy old people for controlled object .The levels of serum hs-CRP,UA and correlation was analyzed.Results The serum hsCRP,UA in the myocardial infarction group were (34.67 ±12.18)mg/L, (412.46 ±68.93)μmol/L,which in the unstable angina pectoris group were (17.21 ±8.39) mg/L,(274.19 ± 46.62)μmol/L,which in stable angina pectoris group were (5.25 ±1.40) mg/L,(297.41 ±37.43)μmol/L,which in the health group(4.96 ±1.24)mg/L,(279.64 ±34.78)μmol/L,the differences among the myocardial infarction group,unstable angina pectoris,stable angina pectoris group had statistical significance (P<0.05), the differences between myocardial infarction group and health group had statistical significance (P<0.05);Myocardial infarction patients prognosis group hs-CRP,UA group was obviously higher than that of the prognosis was good ,compared with statistical significance difference ( P <0.05 );In myocardial infarction group , unstable angina pectoris group , the hsCRP was associated with positive correlation exists between UA (P<0.05),stable angina pectoris group no correla-tion(P>0.05);Different prognosis in patients with myocardial infarction hsCRP is associated with UA has positive correlation between ( P<0.05) .Conclusion Elderly acute myocardial infarction patients serum hs-CRP,UA obviously increased ,and illness development correlation ,a high level of hs-CRP,UA patients often indicates poor prognosis .

Objective:To compare and analyze the volatile components in different medicinal parts of silktree Albizzia julibrissin and their relative contents .Methods:Chromatography-mass spectrometry using headspace solid phase microextraction combined with gas method ( HS-SPME-GC-MS) was used to analyze the volatile components in the bark and flowers of silktree Albizzia julibrissin and the relative contents were calculated by a peak area normalization method .Results:In the bark and flowers of silktree Albizzia julibris-sin, totally 30 and 33 compounds were respectively identified , which respectively accounted for 73.20%and 88.29%of the total vola-tile.A total of 15 components, including alkanes, alkenes, aldehydes, furans, alcohols and acids were in the bark and flowers of silk-tree Albizzia julibrissin, among them, the content of cineole was the highest (bark:15.02%and flowers:21.9%).Conclusion:The HS-SPME technique can be used to analyze and compare the volatile components in the bark and flowers of silktree Albizzia julibrissin, which provides scientific basis for the reasonable development and application of the bark and flowers of silktree Albizzia julibrissin.

Objective To establish a method for the determination of arctiin in Pifu Zhiyang Tablets by SPE-HPLC . Methods SPE-HPLC method was performed on a Kromasil C18 column(150 mm?4.6 mm ,5 ?m),the mobile phase consisted of methanol -1 %acetic acid (35 ∶65) ,the wavelength was 283nm . Results The linear range of arctiin was 1.5～4.5 ?g(r= 0. 999 8) ,the average recovery was 98.18 %with RSD of 2.08 %. Conclusion This method is accurate ,reliable and reproducible. It can be used for the determination of arctiin in Pifu Zhiyang Tablets .

Objective To optimize extraction technology for volatile oil of Pogostemon cablin in the second traditional veterinary drugs anti-stress granule.Methods The single factor was used to investigate the concentration and amount of the ethanol before inclusion of volatile oil.The L9(34) orthogonal design was used to optimize the factors in the process of inclusion.Results The optimal of technology method was that volatile oil was dissolved with 95% EtOH by 1:20(mL/mL),the ratio of oil and ?-CD was 1:8,?-CD was prepared the concentration of 10%,ultrasound pool was at 40 ℃,2.0 h.Conclusion The optimized process is stable and adjustable quality.

6,7-dicho- methoxyl-8-Hydroxycoumarin. Fraxinus rhynchophylla Hance extract had obvious bacteriostasis to 9 kinds of bacterium;monomer mixture and coumarin fingerprint region sample had obvious bacteriostasis to Staphylococcus aureus, Staphylococcus epidermidis, beta streptococcus, aeruginosus Bacillus, Bacillus coli, Klebsiella pneumoniae, catarrh. Unknown component fingerprint region sample had obvious bacteriostasis to Staphylococcus aureus and certain bacteriostasis to Staphylococcus epidermidis, alpha streptococcus, beta streptococcus, Bacillus coli, salmonella. Conclusion The changes of ash bark bacteriostasis were basically identical with known coumarin component content. Aglycone bacteriostasis of ash bark was superior to glycoside, fraxetin and aesculetin bacteriostasis were most powerful in 5 kinds of coumarin monomer.