Brain Neoplasms ; pathology ; Humans ; Pituitary Neoplasms ; pathology

Objective To isolate and cultivate hepatic stem cells(HSCs) from rat fetal liver in vitro and identify their biological features.Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.The cell surface antigen expression of HSCs was observed with immunocytochemical method under confocal laser scanning microscope.Results The isolated HSCs from rat fetal liver grew to monolayer 5 d after cultivation in vitro.They presented pykno-round cells and distinct borderline under the light microscope.After 8 d the cells grew like epithelium.These cells expressed AFP antigen,CK 18 and CK19.Conclusion The cultivated cells are proved to be HSCs and can proliferate quickly in vitro.

95% purity were treated for 24 h with 1,5,10 and 20 ?mol/L 5-azacytidine to induce cellular differentiation.Expression of the cardiac-specific marker-troponin Ⅰ and desmin,identified by immunohistochemistry,was used to identify cardiac muscle differentiation.Results:hMSCs expressed a high level of CD44(hMSCs 93.26%?2.48% vs 3.42%?1.09% in a control group,P

Objective To detect the genetic amplification and protein expression characteristics of aurora-A in ovarian cancer and to interpret the role of aurora-A gene in course of onset,progression and regression phases of ovarian cancer.Methods The amplification of aurora-A gene was detected by quantitative PCR in 6 normal ovarian tissues and 8 ovarian cancer samples,and its protein expression was examined by immunohistochemistry in 6 normal ovarian tissues and 40 ovarian cancer samples,furthermore,the relationships between over-expression of aurora-A protein in ovarian cancer tissue and its pathologic classification,tissue differentiation,clinical phase,tumor proliferation trait and prognosis were analyzed.Results Quantitive PCR showed that aurora-A mRNA was significantly higher in 8 ovarian cancer samples than that in normal ovarian tissues(P0.05).Conclusion There are abnormal amplification and protein over-expression of aurora-A in ovarian cancer tissue,aurora-A probably play an important role in the onset and progression of ovarian cancer,and the novel biological treatment concerning aurora-A gene and its protein is probably a useful route for curing tumor.

Bone Neoplasms ; pathology ; Cerebellum ; pathology ; Humans ; Neurocytoma ; pathology ; Petrous Bone ; pathology

Humans ; Lymphoma, Large B-Cell, Diffuse ; pathology ; Pituitary Gland ; pathology

Abdominal Pain ; etiology ; Acute Pain ; etiology ; Child ; Humans ; Lymphoma ; complications

Objective:To explore the correlation between serum soluble T cell immunoglobulin-mucin molecule-3 (sTim-3), galactose lectin-9 (Galectin-9) and inflammatory factors interferon-γ (IFN-γ), interleukin-4 (IL-4) in patients with brucellosis.Methods:Ninety patients with brucellosis admitted to Yantai Yeda Hospital from January 2016 to December 2018 were selected as the observation group, of whom 49 cases in acute stage and 41 cases in chronic stage. Another 50 healthy subjects who underwent health examination during the same period were selected as the control group. Five milliliter of fasting venous blood samples were collected from patients with brucellosis and healthy subjects, and enzyme-linked immunosorbent assay (ELISA) was used to detect sTim-3 and Galectin-9 levels, and cytometric bead array (CBA) to detect IFN-γ and IL-4 levels; Pearson correlation test was used to correlate serum levels of sTim-3, Galectin-9, sTim-3/Galectin-9 and inflammatory factors IFN-γ, IL-4 in patients with brucellosis.Results:The levels of sTim-3 [(18.28 ± 5.38), (7.01 ± 1.97) ng/L], Galectin-9 [(2.79 ± 0.94), (1.67 ± 0.23) ng/L], sTim-3/Galectin-9 (6.55 ± 2.96, 4.20 ± 0.67), IFN-γ [(5.99 ± 1.94), (10.09 ± 2.03) ng/L], IL-4 [(7.34 ± 3.13), (3.71 ± 1.76) ng/L] were significantly different between the observation group and control group ( t = 14.271, 8.276, 5.527, 11.785, 7.556, P < 0.05). In the observation group, the levels of sTim-3 [(22.09 ± 3.13), (14.21 ± 3.42) ng/L], Galectin-9 [(2.98 ± 0.67), (2.41 ± 0.74) ng/L], sTim-3/Galectin-9 (7.41 ± 1.07, 5.90 ± 1.72), IFN-γ [(3.78 ± 1.65), (7.02 ± 1.73) ng/L], and IL-4 [(9.17 ± 2.04), (5.67 ± 1.54) ng/L] were statistically significant difference between patients with acute and chronic brucellosis ( t = 11.402, 3.833, 5.084, 9.075, 9.037, P < 0.05). Pearson correlation test showed that the levels of sTim-3, Galectin-9, and sTim-3/Galectin-9 in patients with brucellosis were negatively correlated with IFN-γ level ( r = - 0.568, - 0.531, - 0.519, P < 0.05), and positively correlated with IL-4 level ( r = 0.517, 0.524, 0.536, P < 0.05). Conclusion:The expressions of sTim-3 and Galectin-9 in serum of brucellosis patients are high, and they are significantly correlated with IFN-γ and IL-4 levels, suggesting that sTim-3 and Galectin-9 maybe related to the occurrence and development of brucellosis.

Objective: To explore the distribution and intensity of kitchen waste treatment plant, providing scientific basis for occupational diseases prevention and treatment in that industry. Methods: 30 operating personnel in a kitchen waste disposal enterprise were selected as the research group and 16 non-operating personnel as the control group. The epidemiological survey was carried out in June 2015 and July 2016, and the results of occupational hazards and occupational health monitoring during 2015 to 2017 were analyzed. Results: The results of various occupational hazards factors during 2015 to 2017 were all in accordance with the occupational health limit, but the results of occupational health examination were abnormal. The occupational correlation could not be ruled out by the abnormality of electrical audiometry, electrocardiogram and Department of Dermatology examination. Conclusion Workers in kitchen waste treatment plant are likely to suffer low-intensity, continuous health damage. There is occupational health risk in that industry. The accumulation of basic information and the improvement of relevant standards and methods are needed for further study.

Purpose To investigate the clinicopathological features,immunophenotypes,molecular characteristics,treat-ment and prognosis of nodal follicular helper T cell lymphoma,angioimmunoblastic type(nTFHL-AI)with B cell clonal hyper-plasia.Methods The clinicopathological data of 10 nTFHL-AI patients with B cell clonal hyperplasia were collected from medi-cal records,with HE and immunohistochemical staining and gene rearrangement analysis.Related literature was also re-viewed.Results The included 10 patients were 5 males and 5 females with a median age of 73 years.The clinical manifesta-tions were mainly systemic lymphadenopathy,splenomegaly and B symptoms.8 patients were categorized as stage Ⅳ and 2 pa-tients were staged as Ⅰ+Ⅱ according to Ann Arbor staging cri-teria.Major laboratory results were increased β2 microglobulin,lactate dehydrogenase and decreased hemoglobin,erythrocytes and thrombocytes.Plasma Epstein-Barr virus(EBV)nucleic acid quantification was positive in 8 cases.Microscopically,the morphological patterns were nodular aggregation or scattered clear cells,branched high endothelial vessels and disorderly"wind-blown"like follicular dendritic cells(FDC).The num-ber of infiltrated eosinophils was 0-5/HPF in 7 cases,5-10/HPF in 2 cases,and＞50/HPF in 1 case.Plasma cell count was≤5％in 6 cases,10％in 1 case,20％in 1 case,and the rest 2 had relative higher count of 30％.The tumor cells of 7 ca-ses coexisted with marked hyperplasia of histiocytes,and in only one case Reed-Sternberg(RS)-like large cells were found 5 ca-ses showed abundant background B cells while the other 5 cases had limited background B cells.All tumor cells expressed T cell markers,6 were positive for CD10,BCL6,CXCL13 and PD-1 simultaneously and all of 10 were positive for BCL6,CXCL13 and PD-1.8 cases were positive for EBV-encoded small RNA(EBER)by in situ hybridization.Clonal TCR gene rearrange-ments and IG gene rearrangement were detected in all 10 pa-tients.After diagnosis,all patients were treated with chemother-apy,and three of them died due to disease progression.Conclu-sion B cell clonal hyperplasia,the status of EBV infection and the number of EBV-positive cells may be related with disease progression,individualized treatment and prognosis of patients with nTFHL-AI.