Objective To observe the effect of acupuncture plus transcutaneous electrical acupoint stimulation (TEAS) on the range of motion and pain in stagnation-induced periarthritis of shoulder.Method A hundred patients with stagnation-induced periarthritis of shoulder were randomized into a treatment group and a control group, 50 cases each. The treatment group was intervened by acupuncture plus TEAS, while the control group was intervened by acupuncture alone. The interventions were given once every other day, 10 sessions as a treatment course, for 1 course in total. Before and after the intervention, the Visual Analogue Scale (VAS) was adopted for evaluating pain before and after the treatment, and the motor function scale of shoulder was used to measure the range of motion of shoulder joint. The clinical efficacies were also compared between the two groups.Result After the intervention, the VAS scores and shoulder motor function scores were significantly changed in both groups (P<0.05); the VAS score in the treatment group was significantly different from that in the control group after the treatment (P<0.05). The markedly effective rate was 74.0% in the treatment group versus 50.0% in the control group, and the between-group difference was statistically significant (P<0.05).Conclusion Acupuncture plus TEAS can produce a significant efficacy in treating periarthritis of shoulder, as it can obviously improve the range of motion and pain.

Application research on human amniotic membrane has been carried out for nearly a hundred years and people found that there were more than dozens of kinds bioactive substances in the amniotic membrane. It has been proved that the amniotic membrane has a lot of functions, such as anti-inflammatory, anti-bacterial, anti-virus, anti-angiogenic and promoting cell apoptosis, and soon. As effective treatments, amniotic membrane has been used for adjunctive therapy of burns, trauma, ophthalmic damage, dermatopathya. Recent advances of amniotic membrane and amniotic membrane-derived cells research have led to enormous progress in skin tissue engineering, vascular tis- sue engineering, biological scaffold material, and biological sustained-release materials. Amniotic membrane and amniotic membrane derived cells have a significant advantage and unique charm in medical field. Therefore, they have higher research value and broad prospects in the applications.
Amnion ; Biomedical Research ; trends ; Humans ; Tissue Engineering ; Treatment Outcome

ObjectiveTo investigate effects of the arterial blood collection from radial artery,brachial artery,femoral artery and orsalis pedis artery for gas analysis of infants.Methods Three hundred and fifty two infants receiving blood collection were divided into four groups radial artery (n=92),brachial artery (n=94),femoral artery (n=73) and dorsal artery (n=53).Comparisons were done between them in terms of one-time success rate of puncturing and rate of complications from puncturing.Result The radial artery group had lowest rates of hematoma and mistaken puncturing into veins (P<0.05) and higher rate of one-time successful puncturing (P<0.001).Conclusion The radial artery is the first choice for infants undergoing arterial puncturing.

Objective To analyze the outcome of reoperation in drug resistant epilepsy after failed surgery or recurrence in children,and to identify the predictors of the outcome of reoperation and inspiration of epilepsy resection surgery.Methods Analysis was done on data of 4 patients with drug resistant epilepsy diagnosed in Department of Neurosurgery Epileptic Center,Fuzhou General Hospital of Nanjing Military Command from Sep.2011 to Sep.2012.Clinical manifestation,preoperative evaluation,postoperative follow-up of the children were analyzed.Results After first epilepsy resection surgery,3 patients had little decrease in seizure rate(Engel class Ⅳ),the other one had seizure freedom (Engel class Ⅰ),but got recurrence after 2 years.Four cases were all underwent extended resection reoperation du-ring the study period.Types resection included temporoparietooccipital (3/4 cases) and frontal (1/4 cases),mean follow-up 11.25 months.One of the 4 patients had seizure freedom (Engel class Ⅰ),and 3 cases of the 4 patients had seizure reduction (Engel class Ⅱ) at last follow-up,in addition,none of them had significant or unacceptable complication.Conclusions Patients with recurrent drug resistant epilepsy after an initial resection surgery can regain seizure freedom outcome when they underwent repeat resection surgery after comprehensive reevaluation.Predictors with likelihood of seizure freedom after reoperation are:(1) retained medial temporal structures or relevant magnetic resonance imaging structural lesion;(2) original surgery suboptimal; (3) preceding evaluation suggests solitary identified focus;(4) semiology relate to preoperative seizures.

OBJECTIVE:To investigate the application situation and tendency of antihypertensive drugs in our hospital. METHODS: By a retrospective study,a total of 952 prescriptions of outpatients with hypertension were sampled from our hospital from Nov. 18 to Dec. 17 in 2007 for statistical analysis regarding the utilization of antihypertensive drugs,the treatment regimens,sales amount,DDDs and DDC,etc. RESULTS: The antihypertensive drugs were mainly used in single or two kinds concomitantly. Calcium-channel blocker (CCB) and angiotensin receptor Ⅱ binders(ARB) took the lead,accounting for 30.67% and 28.45%,respectively. Leading the first three places on the list of DDDs were Telmisartan,Amlodipine and Benidipine,and leading the first three places on the list of sales amount were Telmisartan,Valsartan and Amlodipine. CONCLUSION:The use of antihypertensive drugs in our hospital conforms to China Guidelines on Prevention and Management of Hypertension and the drug use criteria recommended by WHO.

Objective To investigate the biological properties of human amniotic mesenchymal stem cells (hAMSCs) which were preconditioned with phosphodiesterase-5 inhibitor (Vardenfil). Methods hAMSCs were in vitro isolated and cultured, hAMSCs were pre-treated with vardenfil in final concentration of 10 μmol/L. The morphology of Vard-hAMSCs was observed, and the immunological characteristics, proliferative capacity, and ability of anti-oxidative damage of hAMSCs and Vard-hAMSCs were analyzed by flow cytometry. Double labeling immunofluorescent staining was used to count the differences of differential potential between neural cells of hAMSCs and Vard-hAMSCs. Results (1)Flow cytometry revealed that both hAMSCs and Vard-hAMSCs positively expressed CD90、CD105 and CD73, and negatively expressed CD34、CD45、CD11b and HLA-DR. The SPF and PI in Vard-hAMSCs group were (0.57 ± 0.40)% and (2.20 ± 1.60)% respectively, there was no statistical significance compared with hAMSCs group; (2)After 4 hours treated by H2O2, the apoptosis rate in Vard-hAMSCs group were (7.67 ± 0.82)%,which were markedly lower than that in the hAMSCs group and specific blocker group; (3)Under the same induction condition, positive rates of MAP-2 and GFAP in Vard-hAMSCs group were (49.8 ± 6.42)%and (55.2 ± 6.10)% respectively detected by double labeling immunofluorescent staining, which were significantly higher than the control group. Conclusion The strategy that hAMSCs are treated with vandenfil can enrich the ability of anti-oxidative damage and the differential potential for neural cells in a certain time, and the morphology, immunological characteristics, proliferative capacity of Vard-hAMSCs have no significant change. It suggests that pre-treatment with vandenfil may provide a optimized experimental strategey for hAMSCs which were used to treat nervous system disease.

Objective:To develop an HPLC-MS/MS method for the determination of rosuvastatin in plasma and study the relative bioavailability and bioequivalence of the capsules and tablets in Chinese healthy volunteers. Methods: A single oral dose (20 mg of the test or reference preparation) was given to 24 male healthy volunteers in a randomized crossover study. The plasma concentration of rosuvastatin was determined by HPLC-MS/MS. The pharmacokinetic parameters were calculated and the bioavailability and bioequiva-lence of the two preparations were evaluated by DAS 3. 0 software. Results:After a single dose, the pharmacokinetic parameters of ro-suvastatin capsules and tablets were as follows:Tmax was (3. 56 ± 1. 68) h and (3. 63 ± 1. 56) h, Cmax was (21. 17 ± 13. 74) ng· ml-1 and (26.33 ±23.22) ng·ml-1, t1/2 was (10.68 ±5.50) h and (9.04 ±6.00) h, AUC0-t was (219.31 ±146.09) ng·h· ml-1 and (252. 43 ± 194. 96) ng·h·ml-1 , AUC0-∞ was (225. 32 ± 146. 76) ng·h·ml-1 and (257. 24 ± 194. 61) ng·h·ml-1 , respectively. The 90% confidential interval of AUC0-t, AUC0-∞ and Cmax was 81. 1%-106% , 81. 8%-105. 4% and 77. 9%-104. 5%, respectively. The mean relative bioavailability of the test preparation(the capsules) to the reference preparation(the tablets) was (100. 7 ± 54. 1)%. Conclusion:The test and reference preparations are bioequivalent.

Objective To investigate the significance of microsatellite instability(MSI) and expressions of cyclin D1 and P53 in 7,12 dimethylbenz(a)anthracene (DMBA) induced breast carcinoma in rats. Methods Animal model of rat breast carcinoma was duplicated in 30 female Wistar rats by gastric irrigation of DMBA at the dose of 200 mg/kg. Eight microsatellite loci(D1Mitl4, D1Mgh2, D1Mgh6, D3Mit3, D3Mgh9, D5Mitll, D5Mgh5 and D7Mgh1) were selected for the examination of MSI of breast carcinoma by PCR analysis. S P immunohistochemical staining was used to study the expressions of cyclin D1 and P53 in DMBA induced breast carcinoma in rats. Results Of the 27 cases of breast carcinoma, there were 2 cases of MSI at 8 loci, 9 cases with P53 overexpression and 12 cases with cyclin D1 overexpression. All these were associated with histological grading. Conclusion MSI is infrequent in breast carcinogenesis in rats. Cyclin D1 overexpression may be the early event but P53 protein overexpression may be the late event in breast carcinoma.

OBJECTIVE:To discuss the way for clinical pharmacists to carry out pharmaceutical care.METHODS:We summarized the content,method and experiences of carrying out pharmaceutical care based on our clinical practice in Cardiology department.RESULTS & CONCLUSION:To provide all-round high quality pharmaceutical care for patients,clinical pharmacists should improve their own qualities by continuous learning knowledge and theories related to their own profession as well as improving their practical skills.

Objective To establish a simple and reliable experimental rodent model sensitive to coxsackievirus A16 ( CVA16) .Methods Mongolian gerbils with different age were selected and inoculated intraperitoneally with live CVA16, and the gerbils were observed daily until 14 days postinoculation to screen for the most optimal ages sensitive to the virus.The dose-dependent symptoms were evaluated and the 50% lethal dose (LD50) was determined.The virus titers were measured in blood and various tissues of CVA 16-infected Mongolian gerbils 3 days post-infecton.Finally, the gerbils were immunized twice with inactivated CVA 16 vaccine at day 1 and day 11, respectively, followed by challenge with the virus with a dose of LD50 at day 14.The gerbils were then observed for another 2 weeks to record their body weight , symptom and mortality rate .Their blood samples were collected from the eyes , and CVA16-specific neutralizing antibodytiters and total antibody titers was checked by microneutralization test and ELISA , respectively .Results Various clinical symptoms, such as inactivity, hind limb weakness, paralysis and even death occurred in gerbils following CAV 16 infection. 7-day-old and 14-day-old gerbils are susceptible to CVA 16 infection whereas 28-day-old gerbils are resistant .The most sensitive and appropriate age is 14-day-old.The 50%lethal dose was determined to be 1×104.5 CCID50.High titers of the virus were confirmed in blood and various tissues of Mongolian gerbils contracted CAV 163 days post-infecton.The survival rate is 87.5%for 14-day-old gerbils preimmunized with two doses of inactivated CVA 16 vaccine and challenged with the virus.The geometric mean titers ( GMTs) of neutralizing antibody was 28.14, and the seroprevalence was 87.5%.Conclusions Mongolian gerbils is sensitive to CVA16 and the virus reproduces actively in Vivo.Thus, it can be used as a reliable small animal model for studies of CVA 16 pathogenesis , vaccine development and drug evaluation .