The research carried out a questionnaire regarding education of medical morality at four medical colleges in Fujian province, it found some problems existed, for example, the education of medical morality is ful-filled without knowing medical classical works, being short of systematicness, having no reviewing and encouraging mechanism. The article discusses the cause of the problem. ,with the reality of theInternet +, the characteris-tics of the medical students′education pattern and the characteristics of the medical ethics education.

The goal of medical education is to train good doctors.Only by understanding the trend of medical education development,call medical schools effectively promote educational reform and improve the quality of medical education.In this paper,we analyze the trend of course plans,teaching content,teaching strategies and the evaluation mode of the domestic and international medical education reform and development,introduce the ideas and practices in reform of management system,training pattern,course plans and assessment methods based on the education platform of"mutual promotion of teaching and research,mutual integration of medicine and humanities and simultaneous improvement of knowledge and ability"in Chongqing Medical University.

Objective To explore the diagnostic value of electroneurophisology in patients with charcot-Ma-rie-Tooth disease(CMT).Methods Electromyogram(EMG),Motor nerve conduction velocity(MCV),Sensory nenre conduction velocity(SCV) and F wave were measured in 31 cases with Charcot-Marie-Tooth disease(CMT).Resuits The abnormality rate of EMG was 90%,MCV was 100%,SCV was 90%,and F wave was 34%.Conclu-sion The electroneurophysiology is very practical and valuable in diagnosing Charcot-Marie-Tooth disease.

Objective To explore the diagnostic value of electroneurophisology in patients with charcot-Ma-rie-Tooth disease(CMT).Methods Electromyogram(EMG),Motor nerve conduction velocity(MCV),Sensory nenre conduction velocity(SCV) and F wave were measured in 31 cases with Charcot-Marie-Tooth disease(CMT).Resuits The abnormality rate of EMG was 90%,MCV was 100%,SCV was 90%,and F wave was 34%.Conclu-sion The electroneurophysiology is very practical and valuable in diagnosing Charcot-Marie-Tooth disease.

Objective: To screen the receiving solution used to study the transdermal absorption of strychnine in Jiufen spray. Methods: With recovery,Q-T equation and transdermal speed constant as the parameters, the improved Franz-cell and the skin of SD rats were used to screen the receiving solution from saline, saline-alcohol (7∶3,v∶v), pH phosphate buffer solution-alcohol (88∶2,v∶v), pH 7.4 phosphate buffer containing 25% alcohol. Results: The comparison of the inter-day and intra-day recoveries,Q-T equation and transdermal speed constant showed that saline-alcohol(7∶3) was the best for the study. Conclusion: It is indicated that saline-alcohol(7∶3) was the best receiving solution for the transdermal absorption study of Jiufen spray.

The practical teaching system for information management and information system science in higher medical institutions was constructed with job skill training as its target by intruding the concept of competence-based education model into it in order to improve the practical performance of undergraduates majoring in informa-tion management and information system science. The system was designed by taking into consideration the teaching contents, teaching methods and teaching practice, and implemented in the undergraduates of Chengdu Medical College, and the implementation experiences were summarized.

Objective: To study the transdermal releasing rule of the preparation of Jiufen Spray.Methods: Improved Franz diffuser was applied to transdermal experiment with TLC scanning method.Results: Q T (quantum time) equations of Jiufen Spray are strychnine:Q=220.941t- 486.006 , brucine:Q=208.146t-454.629, ephedrine:Q=177.691t-247.826. The study on releasing rule suggests the accumulative amount of transdermal drug increases with time, but the releasing speed is roughly stable, the total releasing ratio of 12 hours is about 50%. Conclusion: Jiufen spray could surmise to maintain relatively stable plasma drug concentration.

Objective This study was carried out to investigate the effect of myocardial insulin resistance on expression of p38 mitogen-activated protein kinase (MAPK) in ischemic heart failure in rat.Methods Male sprague-dawley rats were subjected either to ligation of the left anterior descending artery (LAD) (n =24) or to sham operation (n =24).After two weeks,cardiac size and function were determined by echocardiography.Glucose and fatty acid (FAO) oxidation rates as well as insulin response were measured in the isolated working heart.The protein expression of p38MAPK was evaluated by Western blotting.Results The infarcted hearts were dilated and had a reduced ejection fraction (ejection fraction ＜0.50).The basal glucose oxidation was preserved,but the fatty acid oxidation was significantly reduced.Insulin effect on substrate oxidation was significantly impaired for both the decrease in fatty acid oxidation and the increase in glucose oxidation.The protein expression of p38MAPK in infracted hearts wasfisigni cantly reduced(P＜0.05).Condusion Myocardial infarction in rats caused partial insulin resistance at the level of substrate oxidation,which was associated with cardiac contractile dysfunction and the expression of p38MAPK.

Objective To observe tree shrews gastrointestinal glucagon like peptide 1 ( glp-1 ) immune response positive cells ( EG cells) distribution and the difference of the digestive tract of rats.Methods Immunohisto chemical SABC method to detect tree shrews diges-tive tract (except for oral cavity, pharynx) glp-1 cells positive content.Results EG cells distributed in tree shrews duodenum,ileum and colon,glandular epithelial cells of jejunum,positive material is distributed in the cytoplasm.Tree shrews stomach,rectum did not see EG cell distribution.Ileal immune dyeing results show positive,EG cells of the digestive tract,only the ileum and colon distribution glp-1 more posi-tive, tree shrews compared with the rats in the digestive tract glp-1 significant difference (P<0.01).Conclusion Gastrointestinal tree shrew in duodenum,ileum and colon,jejunum have glucagon positive cells.The rat only have positive cells in the ileum and colon.According to the mechanism of the glp-1 and the distribution of tree shrews gastrointestinal glp-1,tree shrews atherosclerosis ( atherosclerosis,AS) AS the model is not easy to make may be associated with the role of glp-1.

BACKGROUND:Human-mammal chimeric liver chimera has been a vital significance for the proliferation and differentiation of bone marrow mesenchymal stem cells.
OBJECTIVE:To establish an animal model of human-rhesus chimeric liver using adult bone marrow mesenchymal stem cells.
METHODS:Adult bone marrow mesenchymal stem cells were isolated, purified and cultured for the sixth generation. The number of bone marrow mesenchymal stem cells was no less than 5×108. Bone marrow mesenchymal stem cells labeled with green fluorescent protein were transplanted into the liver of the embryo rhesus with pregnancy of 10 weeks under guided by type-B ultrasound. At the 1st and 3rd months of birth, the liver tissue of the infant rhesus was taken for biopsy. After routine pathological section, histological specimens were observed under fluorescence microscope to confirm if there were adult bone marrow mesenchymal stem cells positive for green fluorescent protein and their distribution, and detected by immunohistochemical staining to identify if human albumin expressed in the liver of infant rhesus.
RESULTS AND CONCLUSION:Fluorescence microscope observation indicated that at the 1st and 3rd months after birth, there were surviving bone marrow mesenchymal stem cells derived from human with green fluorescence in the liver of infant rhesus, and these cells migrated to form more concentrated distribution. The immunohistochemical results demonstrated that functional liver cells expressing human albumin were observed in the liver of infant rhesus at the 1st and 3rd months after birth, and their distribution was in accordance with bone marrow mesenchymal stem cells with green fluorescence. Human-rhesus chimeric liver can be established using adult bone marrow mesenchymal stem cells, which can generate functional liver cells in the liver of infant rhesus.