1.Impact of RelA antisense oligonucleotides on laryngeal carcinoma Hep-2 cell proliferation.
Song PAN ; Jingzhi WAN ; Lilian WU ; Ji ZHAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2010;24(24):1135-1137
OBJECTIVE:
To study the impacts of RelA antisense oligonucleotides on proliferation in laryngeal carcinoma Hep-2 cell.
METHOD:
RelA antisense oligonucleotides was designed, which was transferred into laryngeal carcinoma Hep-2 cell. MTT was used to detect the growth-inhibiting ratio at different transferred timepoints. Hep-2 cell which was transferred 48 h was used to do colony assay, and expression of RelA was detected by Reverse Transcription PCR and Western blot.
RESULT:
MTT results showed that RelA antisense oligonucleotides could significantly suppress the proliferation of Hep-2 cell, and the suppression-ratio elevated with time. There were statistical difference compared with control groups. The number of cells colony was reduced in RelA antisense oligonucleotides group compared with control groups, which had statistic significance. RT-PCR and Western blot results demonstrated that RelA antisense oligonucleotides could significantly inhibit the expression of messenger RNA and protein in Hep-2 cell.
CONCLUSION
RelA antisense oligonucleotides can inhibit the expression of messenger RNA and protein, and induce the cell proliferation and increase the number of cells colony in Hep-2 cell.
Cell Line, Tumor
;
Cell Proliferation
;
drug effects
;
Humans
;
Laryngeal Neoplasms
;
genetics
;
pathology
;
Oligonucleotides, Antisense
;
genetics
;
pharmacology
;
Transcription Factor RelA
;
genetics
2.Teaching discussion on improving post competence of medical imaging major students based on non-standardized assessment teaching mode
Guangming HE ; Xiaohong BI ; Hui WU ; Huai CHEN ; Lilian TAN ; Xi LI
Modern Hospital 2023;23(12):1936-1938
With the great development of medical education reform,clinical competency has become the requirements and goal of talent training in Medical Imaging in medical universities within and beyond China.The aim of this article is to review the role of non-standardized assessment at raising students'clinical competency in the course of Diagnostic Medical Imaging in our university.To achieve the goal of reinforcing student's thought of medical imaging and their clinical competency,building up high-quality educational evaluation system,strengthening the teaching faculty,facilitating the combination of theory teaching and practice teaching,improving the practice ability and the awareness of services after their formal induction,and therefore cultiva-ting effective and practical medical imaging talents for our nation.
3. Neutrophil to lymphocyte ratio in peripheral blood: a novel independent prognostic factor in patients with head and neck squamous cell carcinoma
Feng WU ; Lilian WU ; Lixin ZHU
Chinese Journal of Oncology 2017;39(1):29-32
Objective:
To investigate whether neutrophil to lymphocyte ratio (NLR) in peripheral blood can be an independent prognostic factor in patients with head and neck squamous cell carcinoma (HNSCC).
Methods:
Clinical data of 97 HNSCC patients who received surgical treatment in our department between January 2008 and January 2012 were analyzed retrospectively. The 97 patients were divided into low NLR group (NLR≤5,