Objective:To investigate the pharmacokinetics of probucol(PB)self-microemulsifying drug delivery system(PB-SMEDDS) in rats.Methods:Rats were used as the models.With PB suspension as control,double cycle crossover with single oral dose was performed to investigate the pharmacokinetics of PB-SMEDDS.The HPLC method was established to detect drug concentration in the rat plasma,and the obtained data were processed with 3P97 software.Results:The plasma concentration-time profiles of PB-SMEDDS conformed to a two-compartment model(the coefficient weight is 1/C2).Cmax,tmax and AUC0～96h were 6.72?g/ml,8.58 h and 253.98(?g.h)/ml,respectively.The AUC0～96h of PB-SMEDDS was 19.08 times of PB suspension.Conclusion:PB-SMEDDS could improve the oral bioavailability of PB significantly.

Agonists of peroxisome proliferator-activated receptor gamma (PPARgamma) are of interest as a treatment of type II diabetes, and indenone derivatives are a new class of non-TZD PPARgamma agonists. Based on existing indenone derivatives, a series of novel ones have been designed and synthesized. Meanwhile the structures have been comfirmed with 1H NMR and MS. Among them, 17b and 19 showed higher agonistic activities than rosiglitazone.

Objective To explore the mechanism of combined treatment with methotrexate (MTX) and Ga-Al-As laser irradiation for rheumatoid arthritis (RA) and to assess the effectiveness of Ga-Al-As laser therapy for RA. Methods Twenty-two patients with RA were randomly and evenly divided into two groups: the treatment group treated with Ga-Al-As laser irradiation combined with MTX and the control group treated with MTX only. Ten age-matched normal subjects were observed as normal controls. The amount of CD4 + CD25 + regulatory T cells in peripheral blood (PB) of the normal controls and that in the PB and synovial fluid (SF) of the 22 patients before and after therapy were counted by flow cytometry. Meanwhile, the amount of prostaglandin E2 (PGE2) in synovial fluid of the patients was measured before and after treatment by enzyme-linked immunosorbent assay(ELISA). Results After combined treatment the clinical symptoms of the patients were improved significantly, and the amount of PGE2in SF decreased significantly. The count of CD4 + CD25 + regulatory T cells in PB of RA patients was ( 3.84 ±3.20) % , compared to ( 10.05 ± 7.04) % in healthy individuals. The count of CD4 + CD25 + regulatory T cells in SF of RA patients was ( 14.89 ± 12.30) % , much higher than that in PB. The count of CD4 + CD25 + regulatory T cells in SF decreased significantly in treatment group compared to control group (P ＜0.05). Conclusion Ga-Al-As laser irradiation eombined with MTX can effectively improve the clinical symptoms of RA patients. It may be related to the decrease of amount of PGE2 and count of CD4 + CD25 + regulatory T cell in PB and SF.

Prevention of infection by the human papillomavirus (HPV) has become a hot research topic since the relationship between the HPV and cervical cancer was confirmed. Persistent infection with HPV and early expression of proteins has an important role in the pathogenesis of cervical cancer. Vaccines that protect against four high-risk types of HPV (-6, -11, -16, -18) have been used worldwide. A bivalent vaccine (HPV-16 and -18) developed by Walvax is in clinical trials. This study reviews progress in ascertainment of the structure and function of the HPV genome, the molecular mechanism of carcinogenesis, and vaccines based on virus-like particles.
Animals ; Carcinogenesis ; Female ; Humans ; Papillomaviridae ; genetics ; immunology ; metabolism ; Papillomavirus Infections ; pathology ; prevention & control ; virology ; Papillomavirus Vaccines ; genetics ; immunology ; Uterine Cervical Neoplasms ; pathology ; prevention & control ; virology ; Viral Proteins ; genetics ; immunology ; metabolism

Objective To describe the impact of administrative penalties for medical disputes on the medical staff, including their perception on the penalty and its impact on their mind and body, professional behavior, career development, etc, and to provide a theoretical basis for health policy making and hospital management. Methods The phenomenological research method was adopted to conduct in-depth interview to 10 hospital staff in Taian who were involved in medical disputes and got punished, and the Giorgi qualitative data analysis was used in the research. Results The interviewees′perception and impacts of the punishment were concluded into five aspects:physiologically, psychologically, cognitively, behaviorally and professional development. Conclusions It is concluded that the current punishment to individuals has caused quite negative effects, and a new mechanism should be established to systematically reconstruct the procedure and reduce the medical disputes to the minimum.

Objective To establish a three-dimensional digitized visible model to meet the clinical and research needs.Methods The head of the volunteer was scanned by a cone beam CT (CBCT).The bone tissue,the facial soft tissue and the teeth were extracted from the cone beam CT raw data according to the different Houns field values.The 3D digital craniofacial model was reconstructed from the three tissues extracted from the CBCT original data.The 3D dental model with high resolution was acquired by scanning the dental cast with the laser scanner.The 3D facial model with texture was acquired by scanning the volunteer's face directly by other laser scanner.With the regis tration algorithm in 3D graphics,the 3D facial model with texture and the 3D dental model with high resolution were transformed to the same coordinate which was of the 3D digital craniofacial model.Results We acquired a complicated 3D craniofacial digital model which combined the teeth model with high resolution,the bone model and the face model with texture.Conclusions The method is able to accurately establish the craniofacial digital model.

Objective To study the changes and roles of serum anticardiolipin antibodies (ACA) and plasma TXB_2, 6-K-PGF_ 1? in acute cerebral infarction (ACI).Methods Serum ACA and plasma TXB_2, 6-K-PGF_ 1? levels were measured by enzyme linked immunosorbent assay (ELISA) in 143 patients with ACI and were compared with 59 healthy controls. The patients with ACA positive in ACI group were further divided into subgroupⅠwith stroke risk factors and subgroup Ⅱ without stroke risk factors. The levels of TXB_2 and 6-K-PGF_ 1? in the two groups were compared.Results The positive rate of ACA in ACI group (46.85%) was significantly higher than that in control group (11.63%)( P

Objective To study the efficacy and safety of intravenous and intra-arterial thrombolytic therapy in acute cerebral infarction(ACI).Methods 65 patients with ACI were randomly divided in two groups:intra-arterial thrombolytic group (n=21) were intra-arterially administed with 250 000-500 000 units urokinase through femoral artery and intravenous thrombolytic group (n=44) were intravenously administed with 1.5 million units urokinase.Before and after treating, their neurological deficit scales were evaluated. The thrombin time (TT), prothrombin time (PT) and fibrinogen were detected before and after thrombolysis 6 h,3 d and 7 d.The CT scan was rechecked after thrombolysis 24 h.Results (1)ESS scales were higher in the intra-arterial infusion group than those in the intravenous infusion group at 2h, 6h, 24h, and 3d (all P

OBJECTIVE To explore the rational antibiotics usage in hepato-biliary department.METHODS The antibiotics used in liver surgery department were investigated and evaluated according to the standards.RESULTS Totally 235 patients (88.01%) among 267 patients from 2005 to 2007 were used antibiotics,of which 18.30% for therapy,81.70% for prophylaxis and from them 83.40% were for systemic use. The specimens from 35 patients among 235 patients with liver disease,were sent to do antibiotic susceptibility test. Most of the patiants were given therapy by experience.CONCLUSIONS The administration department in the hospital should enhance its work by forming institutions and standards,and inspecting regularly to guarantee the safety of antibiotics usage.

Objective]Observe clinical efficacy of herpes zoster treated by the compound radix scutellariae solution wet application. [Method]50 patients were randomly divided into 25 cases of the compound radix scutellariae group and 25 cases of the nitrofurazone group, respectively given wet application of the compound radix scute-llariae solution and nitrofurazone solution, at the same time given intravenousganciclovir 0.3g/time,1 time/d, oral vitamin B1 20mg/time, 3 times/d, oral cobalt amine 0.5mg/time, 3 times/d, one week as a period. Record all patients' pain, skin lesion scores before the treatment and in the course of the third, fifth, seventh day and record the time of herpes stopping hair, blister drying up, blister beginning to scab and the scabby area not less than 50%.[Result] Pain, skin lesion scores of the compound radix scutellariae group were signifycantly lower than those of nitrofurazone group, the time of herpes stopping hair, blister drying up, blister beginning to scab and the scabby area not less than 50% of the compound radix scutellariae group compared with nitrofurazone group in advance, the difference was statistically significant(P<0.05). [Conclusion]Treatment of the compound radix scutellariae solution to herpes zoster had distinct clinical efficacy, having significant advantages in terms of alleviating pain and promoting skin lesions subsided.