Objective To approach the clinical manifestations of childhood cough variant asthma, and summarize the dingnosis and treatment experience. Methods Retroepective analyzed the clinical data of 49 children with cough variant asthma. Results In 49 children patients,male 27 cases,female 22 cases;Age:1 ～3 years old( 11 cases) ,4 ～6 years old(25 cases) ,7 ～ 12 years old( 13 cases) ;Duration:1 ～24 months;Incidence season:wintry spring (31 cases) ,summer(7 cases) ,autumn(11 cases). Diagnoeis:CAV was 8 cases,and 24 cases were misdiagnoeed as bronchitis, 11 cases were misdiagnosed as upper respiratory tract infection,3 cases were misdiagnosed as chronic pharyngitis,2 cases were misdiagnosed as sinusitis,1 case was misdiagnosed as bronchial foreign body. In 49 children patients,32 cases were significantly effective, 15 cases effective and 2 cases noneffective. Conclusion The clinical manifestations of CAV was noepecific,and miscdiagnosis rate was high. Effect of treatment with expansion bronchical and anti-infection methods was good.

Objective To explore the effects of mindful- based stress reduction exercise on the sleep and negative emotion in Primary glaucoma patients. Methods By using random sampling,primary glaucoma patients at the hospital of between September in 2015 and September in 2016. A total of 93 patients were divided into intervention group and control group according to random number table.The control group was given conventional nursing, intervention group joined in mindful-based stress reduction exercise on the basis of routine nursing. Baseline levels of anxiety, depression and status of sleep were measured in the two groups after admission, evaluated and comparison at the end of formal intervention. Results At the end of intervention, the score of Hamilton Anxiety Scale (HAMA) in intervention group was decreased from (13.42 ± 3.56) points to (8.23 ± 2.11) points (t=7.832, P=0.000), the score of Hamilton Depression Scale (HAMD) was decreased from (18.42 ± 4.55) points to (10.31 ± 2.76) points (t=9.517, P=0.000), the effect was better than the control group (t=8.117, 7.671, all P=0.000). And the score of Self-Rating Scale of Sleep (SRSS) was decreased from (30.81±8.35) points to(25.52±6.13) points (t=3.189, P=0.002), the effect was better than the control group (t=2.253, P=0.027). Conclusions Mindful-based stress reduction exercise can reduce negative emotions such as anxiety and depression and promote the status of sleep effectively.

An improved method for the purification of 1,25-(OH)2D3-26,23-lactone was established by using one step of highperformance liquid chromatography (HPLC). The analytic column was Shim-pack NH2 (straight-phase type). The mobile phase was Methanol: Dichloromethane: Hexane = 4:26:70 and the flow rate was 2ml/min. An ultraviolet detector with 254nm was used. The retention time of 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1, 24, 25-(OH)3D3 werel2.10, 17.06 and 22,00 minutes respectively. This method can separate 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1,24,25-(OH)3D3 only by one step of HPLC and it is better than the old one which need two steps of HPLC for the separation of these substances.

Objective To investigate the relationship between glucose variability and severity of coronary artery stenosis in patients with type 2 diabetes mellitus (T2DM).Methods One hundred hospitalized patients with T2DM(55 males and 45 females,39-81 years old) who had undergone angiography were included in the study from February 2012 to October 2012.They were divided into 2 groups:(1) 60 patients with coronary heart disease(CHD)as experimental group and (2) 40 patients without coronary heart disease as control group.All participants underwent selective coronary angiography to evaluate the severity of coronary artery stenosis (Gensini score) and continuous glucose monitoring(CGM) for 72 h.The correlation was analyzed between biochemical findings,and Gensini scores between the 2 groups.The comparison between 2 groups was performed with LSD test,correlation analysis with Pearson correlation and multiple stepwise regression analysis.Result Compared with the levels of largest amplitude of blood glucose excursion (LAGE),standard deviation of blood glucose (SDBG),mean amplitude of glycemic excursion (MAGE),postprandial glucose excursion (MPPGE),and absolute means of daily differences (MODD) in control group [(8.1 ± 2.1),(1.9 ± 0.5),(4.8 ± 1.3),(4.5 ± 1.4),and (2.4 ± 0.8) respectively],the levels in the experimental group[(11.3 ± 3.5),(3.2 ± 1.2),(7.0 ± 2.6),(6.4 ± 1.9),and (3.1 ± 1.3)] were higher respectively.MPPGE,MODD,LAGE,and C-reactive protein(CRP) were positively related to Gensini score(r were 0.498,0.246,and 0.227,all P＜0.05),while left ventricular ejection fraction (LVEF) was negatively related to Gensini score(r=-0.261,P＜0.05).Multiple stepwise regression analysis indicated that MPPGE and LVEF were independent risk factors of Gensini score (Y =59.727 + 11.779 × MPPGE-1.437 × LVEF).Conclusion Glucose variability in patients with T2DM and CHD was markedly enhanced.MPPGE was positively correlated with the severity of coronary artery stenosis in patients with T2DM and CHD.

Objective To detect the expression of HER2 and TOPOⅡ? in human breast cancer to explore the relationship between them and the potential value in the chemotherapy of breast cancer. Method 24 breast cancer tissues and paired normal tissues were investigated. RT-PCR and imumohistochemical staining were used to detect the expressions of HER2 and TOPOⅡ? at the transcription and protein level respectively. Results HER2 mRNA expressed positively in 29% (7/24) breast cancer tissues vs 4% (1/24) in paired normal tissues. The positive expression of TOPOⅡ? mRNA in breast cancer tissues and paired normal tissues were 92% (22/24) and 21%(5/24) respectively. The result of the immunohistochemical staining showed that the positive rates of HER2 and TOPOⅡ? in breast cancer tissues were 30%(6/20) and 94%(16/17) respectively, which was consistent with RT-PCR. The 6 cancer samples with HER2 positive expression coexpressed TOPOⅡ?. The expression of these two proteins was correlated(R=0.524,P

0.05). The mortality in group A was obviously higher than that in group AI ( P

Objective To explore the feasibility of laparoscopic operation as treatment for early malignant uterine tumors Method Six patients with endometrial carcinoma underwent laparoscopic extensive total hysterectomy and bilateral adnexectomy, and 2 with cervical cancer underwent additional pelvic lymphadenectomy Results The mean operating time of the 6 laparoscopic extensive total hysterectomy and bilateral adnexectomy was 220 min, and mean blood loss was 200 ml The mean operating time of 2 cases underwent additional pelvic lymphadenectomy was 240 min No organ trauma occurred, and the average post operative hospital stay was 8 days Conclusion These results suggest that laparoscopic operation is safe and feasible in treating early stage malignant uterine tumors

Objective To evaluate the application of Endoscopic ultrasonography (EUS)in preoperative diagnosis of gastric cancer and staging. Method 78patients were assigned to EUS and gastroscope examination before operation,the staging result were compared with pathological staging result. Result Compared with pathological staging,the accurate rate of EUS in T staging and N staging before gastric cancer was 85.9% and 80.77%. Conclusion EUS can be used in judgment of gastric cancer staging accurately,may be of significance in the decision of operation plan.

Objective To assess the effect and safety of applying Diver CE thrombus-aspirating device during direct percutaneous coronary intervention(PCI)in pations with ST-segment elevation myocardial infarction(STEMI). Methods Select 46 patients with STEMI who underwent direct PCI between July,2006 and June,2007 in Fushun Central Hospital. Dividing these patients into thrombus-aspirating group and direct PCI group,comparing the difference in chest pain relieving.magnitude of ST-segment declining in 2h,TIMI flow grade 3,the maximum value of CK and CK-MB,EF and MACE outcome(death、myocardial infarction and targert vessel revascularization).Results The percent of chest pain relieving、magnitude of ST-segment declining 、TIMI flow grade 3、the maximum value of CK and CK-MB,and EF in thrombus-aspirating group are piror to the direct PCI group(P0.05).Conclusion applying Diver CE thrombus-aspirating device during PCI can obviously reduce thrombus in coronaty artery and distal embolization,improve myocardial reperfusion more effectively.

To clarify the difference between benign and malignant tumours of tongue on the PC-NA expression and AgNOR c0unts. Methods: Immunohistochemical and AgNOR stainings were carriedout to detect the PCNA expressi0n and to count AgNOR particles in 14 cases of papillomas and 36 cases ofsquamous cell carcinomas of tongue. Results: The positive index(PI) of PCNA was 7. 15 ? 1. 32 in papillo-mas and 24. 28? 2. 47 in squamous cell carcinomas, and there was a significant difference between them. Asignificant difference between low 19. 45? 15. 88, medium 22. 5 ?18. 79 and high grade carcin0mas 65. 3 ?17. 39 was also observed. The AgNOR counts were higher in squamous cell carcinomas 4. 76 ?1. 59 than inpapillomas 2. 16 ? 0. 33. There was no difference of AgNOR counts in high, medium and low grade ofsquamous cell carcinomas. Conclusion:The PCNA expression and AgNOR counts may be related to activi-ty of cell proliferation and be helpful for differentiating benign from malignant tumours of tongue. In addi-tion, the former may be helpful for grading malignancy.