AIM: To investigate the effects of compound salvia miltiorrhiza(SM) injection on lung injury after cardiopulmonary bypass(CPB).METHODS: Twenty-four rats were randomly divided into control group,CPB group and SM group.Cardiopulmonary bypass was made in rats of CPB group and SM group.Lung tissues of all rats were taken 60 min after the weaning of CPB for the assay of malondialdehyde(MDA),myeloperoxidase(MPO),water content of tissue, the observation of pathological changes were made under optical microscope.RESULTS: Remarkable pathohistological and biochemical changes were detected 1 h after CPB.Compared with control group,MDA in lung tissue were increased in CPB group and SM group(P

Objective:To find the influence of the immuno-suppressive drug on the incidence and genotype of oral Candida albicans.Methods:51 patients using immuno-suppressive drug for 1 to 3 months were enrolled,the controls were 26 health subjects.Oral rinse samples from the patients and the controls were assessed for the growth of yeast.The liquid samples were plated on CHROMagar Candida.The isolates were defined to the species level and the genotypic subgroups by PCR method.PCR results were proved by sequencing.Results:The incidence of Candida albican in the patients and controls was 20% and 7.69% respectively.The genotype of the Candida albican in the controls is A subgroup,that in the patients included A,B and C subgroups.Conclusion:Long term of immuno-suppressive drug use can influence the genotype and incidence of oral Candida albican.

Objective To study cervical human papillomavirus (HPV) infection and genotype distribution characteristics in patients with vaginal disease.Methods The PCR dot blot was used to carry out HPV detection of cervical exfoliated cells in 816 women with cervical lesions in the hospital, and 23 kinds of HPV genotypes were identified.Results Among the 816 cases receiving HPV type detection, HPV of 532 cases (65.20%) was positive, including 371 cases (69.74%) with low-risk HPV (HR-HPV) infection, 161 cases (30.26%) with low-risk HPV infection (LR-HPV) (P<0.05).The HPV infection rate below 45 years old was relatively higher.The HPV infection rate in patients with cervical cancer (97.10%) was higher than that in patients with cervical intraepithelial neoplasia (CIN) II -III (80.54%), patients with CIN Ⅰ(41.55%) and patients with cervicitis (17.20%) (P<0.05).The top five types of HPV infection were HPV16, 58, 18, 11 and 56 type, respectively.The single infection rate (79.70%) was higher than multiple infection rate (20.30%) (P<0.05); HR-HPV infection was the main infection in single infection, and double infection was the main infection in multiple infection.Conclusion Below 45 years old women are the high-risk groups of HPV infection.16, 58, 18, 11 and 56 are the main types of HPV infection.The infection rates of patients with cervical cancer and precancerous lesions are relatively higher.Paying attention to the screening of HPV infection has positive significance in the prevention and treatment of cervical cancer.

Objective To observe the expression of P2X receptors in the hippocampus of telomerase gene knockout mice after acupuncturing the bilateral ST36 acupoints.Methods Eighteen telomerase gene knockout homozygous mice were randomly divided into a blank control group (group A),a manual acupuncture group (group B) and an electric acupuncture group (group C),each of 6.Group A was not given any intervention,while groups B and C were provided with manual or electric acupuncture at point ST36 for 7 days.The expression of P2X receptors and cAMP response element bound protein (CREB) in the hippocampus were observed and compared among the 3 groups.Results The expression of P2X4 in the hippocampus was not significantly different among the 3 groups in groups B and C decreased significantly compared with group A,but there were no significant differences between group Bs and C.The expression of CREB in group C decreased significantly compared with groups A and B.Conclusion EA inhibits the expression of P2X4 receptor and CREB the hippocampus.The signaling pathway may play an important role in the mechanism of action of acupuncture in neurodegenerative diseases.

Objective To discusse the effect of edaravone for brain tissue oxidative damage after cerebral hemorrhage through observing the hemorrhage rats back edema and the change of lipid peroxidation.Methods 150 healthy and clean male SD rats, were randomly divided to sham operation group,control group,treatment group,50 cases in each group,each group choose five time points,post operative 0.5 day,1 day,2 days,3 days, 4 days,observed different 10 rats at each time point.The intracerebral hemorrhage model was made by using the method of stereotactic autologous blood injection.The changes of water content, the content of MDA and the activity of superoxide dismutase were monitored in three groups at different time points.Results Surrounding the hematoma in the brain tissue around the hematoma after intracerebral hemorrhage in sham operation group,treatment group of postoperative day 1-4 days each time hematoma surrounding brain tissue water content was significantly lower than that in control group(P<0.05);Control group after 2 days of cerebral edema and reached the highest value;Sham operation group and treatment group of postoperative half of the day to 4 days each time the content of MDA were significantly lower than in control group ( P <0.05 );Sham operation group and treatment group postoperative half of the day to 4 days each time the brain tissue SOD activity was significantly higher than control group(P<0.05).Conclusion The effect is obvious that edaravone for cerebral hemorrhage rats brain tissue around hematoma edema, reduce cerebral hemorrhage rat tissue oxidative damage.

Angiogenesis plays a very important role in the development and metastasis of breast cancer. Low-dose metronomic chemotherapy based on the traditional chemotherapy drugs has antiangiogenic effect. As far as its mechanism, low toxicity or low incidence of drug resistance is concerned, it is feasible for breast cancer therapy. It displayed the validity and feasibility in the pre-clinical studies and clinical trials, which provided a new treatment strategy for the breast cancer.

Objective To explore the diagnostic value of electroneurophisology in patients with charcot-Ma-rie-Tooth disease(CMT).Methods Electromyogram(EMG),Motor nerve conduction velocity(MCV),Sensory nenre conduction velocity(SCV) and F wave were measured in 31 cases with Charcot-Marie-Tooth disease(CMT).Resuits The abnormality rate of EMG was 90%,MCV was 100%,SCV was 90%,and F wave was 34%.Conclu-sion The electroneurophysiology is very practical and valuable in diagnosing Charcot-Marie-Tooth disease.

Objective To explore the diagnostic value of electroneurophisology in patients with charcot-Ma-rie-Tooth disease(CMT).Methods Electromyogram(EMG),Motor nerve conduction velocity(MCV),Sensory nenre conduction velocity(SCV) and F wave were measured in 31 cases with Charcot-Marie-Tooth disease(CMT).Resuits The abnormality rate of EMG was 90%,MCV was 100%,SCV was 90%,and F wave was 34%.Conclu-sion The electroneurophysiology is very practical and valuable in diagnosing Charcot-Marie-Tooth disease.

[Objective]We summarized Professor Zhang Weihua’s clinical experience in treating constipation and diarrhea alternating bowel dysfunction by Chinese medicine. [Method] This paper revealed the professor Zhang Weihua ’s experience of curing constipation and diarrhea alternating bowel dysfunc-tion by analyzing the etiology and pathology ,therapeutic principles and relevant cases .[Results] The etiology and pathology of constipation and diarrhea alternating bowel dysfunction is root deficiency and tip excess ,and cold-heat complex ,the disorders of E. conduction,and the treatment should focus on using warming and heat-clearing simultaneously, tonifying spleen and kidney. [Conclusions] The experience of Professor Zhang Weihua in treating con-stipation and diarrhea alternating bowel dysfunction is effective and thought-provoking in treatment.

Objective:To study the long-term stability of 6MV photon beam on TrueBeam, which is a new linear accelerator introduced by Varian Medical System. Methods:QA BeamChecker Plus (QABC+, Standard Imaging Inc., Middleton, USA) was used to measure 6MV X-ray on TrueBeam linac as daily quality assurance (Daily QA). Dosimetric parameter, including output Constancy, flatness and symmetry, were employed to evaluate the dose delivery stability of TrueBeam linac. Results: Daily QA results of 18 months showed the dose output delta was (0.0%±0.7%), only once out of tolerance of 2%. The constancy of Flatness was excellent with the delta of (0.1%±0.1%). The symmetric of axial and transverse varied within 1%. Conclusion:The output constancy of TrueBeam with 6MV is very stable, as well as flatness and symmetry. It’s stable and reliable to implement Truebeam linear accelerator in clinical.